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Protein

Ornithine aminotransferase

Gene

CAR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 72000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid.

Cofactori

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-ornithine.
Proteins known to be involved in this subpathway in this organism are:
  1. Ornithine aminotransferase (CAR2)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-ornithine, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • identical protein binding Source: GO_Central
  • ornithine-oxo-acid transaminase activity Source: SGD
  • pyridoxal phosphate binding Source: GO_Central
  • zinc ion binding Source: EnsemblPlants

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YLR438W-MONOMER
YEAST:YLR438W-MONOMER
ReactomeiR-SCE-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayiUPA00098; UER00358

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine aminotransferase (EC:2.6.1.13)
Alternative name(s):
Ornithine--oxo-acid aminotransferase
Gene namesi
Name:CAR2
Synonyms:CARGB
Ordered Locus Names:YLR438W
ORF Names:L9753.2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR438W
SGDiS000004430 CAR2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001204991 – 424Ornithine aminotransferaseAdd BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei272N6-(pyridoxal phosphate)lysineBy similarity1
Cross-linki390Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP07991
PaxDbiP07991
PRIDEiP07991

PTM databases

iPTMnetiP07991

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SSA1P105912EBI-12430,EBI-8591

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31696, 67 interactors
DIPiDIP-1225N
IntActiP07991, 28 interactors
MINTiP07991
STRINGi4932.YLR438W

Structurei

3D structure databases

ProteinModelPortaliP07991
SMRiP07991
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00630000089895
HOGENOMiHOG000020206
InParanoidiP07991
KOiK00819
OMAiRTAWDLC
OrthoDBiEOG092C2RJZ

Family and domain databases

CDDicd00610 OAT_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR005814 Aminotrans_3
IPR010164 Orn_aminotrans
IPR034758 Orn_aminotrans_mito
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11986:SF18 PTHR11986:SF18, 1 hit
PfamiView protein in Pfam
PF00202 Aminotran_3, 1 hit
PIRSFiPIRSF000521 Transaminase_4ab_Lys_Orn, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01885 Orn_aminotrans, 1 hit
PROSITEiView protein in PROSITE
PS00600 AA_TRANSFER_CLASS_3, 1 hit

Sequencei

Sequence statusi: Complete.

P07991-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEATLSSKQ TIEWENKYSA HNYHPLPVVF HKAKGAHVWD PEGKLYLDFL
60 70 80 90 100
SAYSAVNQGH CHPHIIKALT EQAQTLTLSS RAFHNDVYAQ FAKFVTEFFG
110 120 130 140 150
FETVLPMNTG AEAVETALKL ARRWGYMKKN IPQDKAIILG AEGNFHGRTF
160 170 180 190 200
GAISLSTDYE DSKLHFGPFV PNVASGHSVH KIRYGHAEDF VPILESPEGK
210 220 230 240 250
NVAAIILEPI QGEAGIVVPP ADYFPKVSAL CRKHNVLLIV DEIQTGIGRT
260 270 280 290 300
GELLCYDHYK AEAKPDIVLL GKALSGGVLP VSCVLSSHDI MSCFTPGSHG
310 320 330 340 350
STFGGNPLAS RVAIAALEVI RDEKLCQRAA QLGSSFIAQL KALQAKSNGI
360 370 380 390 400
ISEVRGMGLL TAIVIDPSKA NGKTAWDLCL LMKDHGLLAK PTHDHIIRLA
410 420
PPLVISEEDL QTGVETIAKC IDLL
Length:424
Mass (Da):46,086
Last modified:October 1, 1996 - v2
Checksum:iA0B6BE492FC5C1B8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8Missing in CAA29947 (PubMed:2824201).Curated1
Sequence conflicti8Missing in CAA29081 (PubMed:3036506).Curated1
Sequence conflicti38V → L in CAA29947 (PubMed:2824201).Curated1
Sequence conflicti38V → L in CAA29081 (PubMed:3036506).Curated1
Sequence conflicti99F → S in CAA29947 (PubMed:2824201).Curated1
Sequence conflicti212G → R in CAA29947 (PubMed:2824201).Curated1
Sequence conflicti385H → Q in CAA29947 (PubMed:2824201).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06790 Genomic DNA Translation: CAA29947.1
U21094 Genomic DNA Translation: AAB67514.1
X05571 Genomic DNA Translation: CAA29081.1
BK006945 Genomic DNA Translation: DAA09738.1
PIRiS59406 XNBYO
RefSeqiNP_013542.1, NM_001182326.1

Genome annotation databases

EnsemblFungiiYLR438W; YLR438W; YLR438W
GeneIDi851158
KEGGisce:YLR438W

Similar proteinsi

Entry informationi

Entry nameiOAT_YEAST
AccessioniPrimary (citable) accession number: P07991
Secondary accession number(s): D6VZ72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health