Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ornithine aminotransferase

Gene

CAR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid.

Cofactori

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-ornithine.
Proteins known to be involved in this subpathway in this organism are:
  1. Ornithine aminotransferase (CAR2)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-ornithine, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • identical protein binding Source: GO_Central
  • ornithine-oxo-acid transaminase activity Source: SGD
  • pyridoxal phosphate binding Source: GO_Central

GO - Biological processi

  • arginine catabolic process Source: SGD
  • arginine catabolic process to glutamate Source: GO_Central
  • arginine catabolic process to proline via ornithine Source: GO_Central
  • L-proline biosynthetic process Source: UniProtKB-UniPathway
  • ornithine metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YLR438W-MONOMER.
YEAST:YLR438W-MONOMER.
ReactomeiR-SCE-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00098; UER00358.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine aminotransferase (EC:2.6.1.13)
Alternative name(s):
Ornithine--oxo-acid aminotransferase
Gene namesi
Name:CAR2
Synonyms:CARGB
Ordered Locus Names:YLR438W
ORF Names:L9753.2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR438W.
SGDiS000004430. CAR2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001204991 – 424Ornithine aminotransferaseAdd BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei272N6-(pyridoxal phosphate)lysineBy similarity1
Cross-linki390Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP07991.
PRIDEiP07991.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31696. 43 interactors.
DIPiDIP-1225N.
IntActiP07991. 9 interactors.
MINTiMINT-403535.

Structurei

3D structure databases

ProteinModelPortaliP07991.
SMRiP07991.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00630000089895.
HOGENOMiHOG000020206.
InParanoidiP07991.
KOiK00819.
OMAiTHDHTIR.
OrthoDBiEOG092C2RJZ.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR005814. Aminotrans_3.
IPR010164. Orn_aminotrans.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PTHR11986:SF18. PTHR11986:SF18. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01885. Orn_aminotrans. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07991-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEATLSSKQ TIEWENKYSA HNYHPLPVVF HKAKGAHVWD PEGKLYLDFL
60 70 80 90 100
SAYSAVNQGH CHPHIIKALT EQAQTLTLSS RAFHNDVYAQ FAKFVTEFFG
110 120 130 140 150
FETVLPMNTG AEAVETALKL ARRWGYMKKN IPQDKAIILG AEGNFHGRTF
160 170 180 190 200
GAISLSTDYE DSKLHFGPFV PNVASGHSVH KIRYGHAEDF VPILESPEGK
210 220 230 240 250
NVAAIILEPI QGEAGIVVPP ADYFPKVSAL CRKHNVLLIV DEIQTGIGRT
260 270 280 290 300
GELLCYDHYK AEAKPDIVLL GKALSGGVLP VSCVLSSHDI MSCFTPGSHG
310 320 330 340 350
STFGGNPLAS RVAIAALEVI RDEKLCQRAA QLGSSFIAQL KALQAKSNGI
360 370 380 390 400
ISEVRGMGLL TAIVIDPSKA NGKTAWDLCL LMKDHGLLAK PTHDHIIRLA
410 420
PPLVISEEDL QTGVETIAKC IDLL
Length:424
Mass (Da):46,086
Last modified:October 1, 1996 - v2
Checksum:iA0B6BE492FC5C1B8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8Missing in CAA29947 (PubMed:2824201).Curated1
Sequence conflicti8Missing in CAA29081 (PubMed:3036506).Curated1
Sequence conflicti38V → L in CAA29947 (PubMed:2824201).Curated1
Sequence conflicti38V → L in CAA29081 (PubMed:3036506).Curated1
Sequence conflicti99F → S in CAA29947 (PubMed:2824201).Curated1
Sequence conflicti212G → R in CAA29947 (PubMed:2824201).Curated1
Sequence conflicti385H → Q in CAA29947 (PubMed:2824201).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06790 Genomic DNA. Translation: CAA29947.1.
U21094 Genomic DNA. Translation: AAB67514.1.
X05571 Genomic DNA. Translation: CAA29081.1.
BK006945 Genomic DNA. Translation: DAA09738.1.
PIRiS59406. XNBYO.
RefSeqiNP_013542.1. NM_001182326.1.

Genome annotation databases

EnsemblFungiiYLR438W; YLR438W; YLR438W.
GeneIDi851158.
KEGGisce:YLR438W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06790 Genomic DNA. Translation: CAA29947.1.
U21094 Genomic DNA. Translation: AAB67514.1.
X05571 Genomic DNA. Translation: CAA29081.1.
BK006945 Genomic DNA. Translation: DAA09738.1.
PIRiS59406. XNBYO.
RefSeqiNP_013542.1. NM_001182326.1.

3D structure databases

ProteinModelPortaliP07991.
SMRiP07991.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31696. 43 interactors.
DIPiDIP-1225N.
IntActiP07991. 9 interactors.
MINTiMINT-403535.

Proteomic databases

MaxQBiP07991.
PRIDEiP07991.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR438W; YLR438W; YLR438W.
GeneIDi851158.
KEGGisce:YLR438W.

Organism-specific databases

EuPathDBiFungiDB:YLR438W.
SGDiS000004430. CAR2.

Phylogenomic databases

GeneTreeiENSGT00630000089895.
HOGENOMiHOG000020206.
InParanoidiP07991.
KOiK00819.
OMAiTHDHTIR.
OrthoDBiEOG092C2RJZ.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00358.
BioCyciMetaCyc:YLR438W-MONOMER.
YEAST:YLR438W-MONOMER.
ReactomeiR-SCE-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

PROiP07991.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR005814. Aminotrans_3.
IPR010164. Orn_aminotrans.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PTHR11986:SF18. PTHR11986:SF18. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01885. Orn_aminotrans. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOAT_YEAST
AccessioniPrimary (citable) accession number: P07991
Secondary accession number(s): D6VZ72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 72000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.