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P07990 (T1S_SALPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-1 restriction enzyme StySPI specificity protein

Short name=S.StySPI
Alternative name(s):
Type I restriction enzyme StySPI specificity protein
Short name=S protein
Gene names
Name:hsdS
OrganismSalmonella potsdam
Taxonomic identifier597 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The StySPI enzyme recognizes 5'-AACN6GTRC-3'.

Subunit structure

The type I restriction/modification system is composed of three polypeptides R, M and S.

Domain

Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.

Sequence similarities

Belongs to the type-I restriction system S methylase family.

Ontologies

Keywords
   Biological processRestriction system
   LigandDNA-binding
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 463463Type-1 restriction enzyme StySPI specificity protein
PRO_0000198038

Sequences

Sequence LengthMass (Da)Tools
P07990 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 1C42A1717F1E85CF

FASTA46351,190
        10         20         30         40         50         60 
MNRGKLPEGW ATAPVSTVTT LIRGVTYKKE QALNYLQDDY LPIIRANNIQ NGKFDTTDLV 

        70         80         90        100        110        120 
FVPKNLVKES QKISPEDIVI AMSSGSKSVV GKSAHQRLPF ECSFGAFCGA LRPEKFISPN 

       130        140        150        160        170        180 
YIAHFTKSSF YRNKISSLSA GANINNIKPA SFDLINIPIP SLAEQKIIAE KLDTLLAQVD 

       190        200        210        220        230        240 
STKARLEQIP QILKRFRQAV LAAAVSGTLT TALRNSHSLI GWHSTNLGAL IVDSCNGLAK 

       250        260        270        280        290        300 
RQGLNGNEIT ILRLADFKDA QRIIGNERKI KLDSKEENKY SLENDDILVI RVNGSADLAG 

       310        320        330        340        350        360 
RFIEYKSNGD IEGFCDHFIR LRLDSNKIMS RFLTYIANEG EGRFYLRNSL STSAGQNTIN 

       370        380        390        400        410        420 
QTSIKGLSFL LPPLKEQAEI VRRVEQLFAY ADTIEKQVNN ALTRVNSLTQ SILAKAFRGE 

       430        440        450        460 
LTAQWRAENP DLISGKNSAA ALLEKIKAER AVSGGKKTSR KKA 

« Hide

References

[1]"Two DNA recognition domains of the specificity polypeptides of a family of type I restriction enzymes."
Fuller-Pace F.V., Murray N.E.
Proc. Natl. Acad. Sci. U.S.A. 83:9368-9372(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M14984 Genomic DNA. Translation: AAA27145.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3689. S.StySPI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view]
PfamPF01420. Methylase_S. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT1S_SALPO
AccessionPrimary (citable) accession number: P07990
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries