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P07990

- T1S_SALPO

UniProt

P07990 - T1S_SALPO

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Protein
Type-1 restriction enzyme StySPI specificity protein
Gene
hsdS
Organism
Salmonella potsdam
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The StySPI enzyme recognizes 5'-AACN6GTRC-3'.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Restriction system

Keywords - Ligandi

DNA-binding

Protein family/group databases

REBASEi3689. S.StySPI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-1 restriction enzyme StySPI specificity protein
Short name:
S.StySPI
Alternative name(s):
Type I restriction enzyme StySPI specificity protein
Short name:
S protein
Gene namesi
Name:hsdS
OrganismiSalmonella potsdam
Taxonomic identifieri597 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Type-1 restriction enzyme StySPI specificity protein
PRO_0000198038Add
BLAST

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Family & Domainsi

Domaini

Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.

Sequence similaritiesi

Family and domain databases

InterProiIPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view]
PfamiPF01420. Methylase_S. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07990-1 [UniParc]FASTAAdd to Basket

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MNRGKLPEGW ATAPVSTVTT LIRGVTYKKE QALNYLQDDY LPIIRANNIQ    50
NGKFDTTDLV FVPKNLVKES QKISPEDIVI AMSSGSKSVV GKSAHQRLPF 100
ECSFGAFCGA LRPEKFISPN YIAHFTKSSF YRNKISSLSA GANINNIKPA 150
SFDLINIPIP SLAEQKIIAE KLDTLLAQVD STKARLEQIP QILKRFRQAV 200
LAAAVSGTLT TALRNSHSLI GWHSTNLGAL IVDSCNGLAK RQGLNGNEIT 250
ILRLADFKDA QRIIGNERKI KLDSKEENKY SLENDDILVI RVNGSADLAG 300
RFIEYKSNGD IEGFCDHFIR LRLDSNKIMS RFLTYIANEG EGRFYLRNSL 350
STSAGQNTIN QTSIKGLSFL LPPLKEQAEI VRRVEQLFAY ADTIEKQVNN 400
ALTRVNSLTQ SILAKAFRGE LTAQWRAENP DLISGKNSAA ALLEKIKAER 450
AVSGGKKTSR KKA 463
Length:463
Mass (Da):51,190
Last modified:August 1, 1988 - v1
Checksum:i1C42A1717F1E85CF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14984 Genomic DNA. Translation: AAA27145.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14984 Genomic DNA. Translation: AAA27145.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3689. S.StySPI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view ]
Pfami PF01420. Methylase_S. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Two DNA recognition domains of the specificity polypeptides of a family of type I restriction enzymes."
    Fuller-Pace F.V., Murray N.E.
    Proc. Natl. Acad. Sci. U.S.A. 83:9368-9372(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiT1S_SALPO
AccessioniPrimary (citable) accession number: P07990
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi