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P07990

- T1S_SALPO

UniProt

P07990 - T1S_SALPO

Protein

Type-1 restriction enzyme StySPI specificity protein

Gene

hsdS

Organism
Salmonella potsdam
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    • Comment

    Functioni

    The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The StySPI enzyme recognizes 5'-AACN6GTRC-3'.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    DNA-binding

    Protein family/group databases

    REBASEi3689. S.StySPI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-1 restriction enzyme StySPI specificity protein
    Short name:
    S.StySPI
    Alternative name(s):
    Type I restriction enzyme StySPI specificity protein
    Short name:
    S protein
    Gene namesi
    Name:hsdS
    OrganismiSalmonella potsdam
    Taxonomic identifieri597 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 463463Type-1 restriction enzyme StySPI specificity proteinPRO_0000198038Add
    BLAST

    Interactioni

    Subunit structurei

    The type I restriction/modification system is composed of three polypeptides R, M and S.

    Family & Domainsi

    Domaini

    Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR000055. Restrct_endonuc_typeI_HsdS.
    [Graphical view]
    PfamiPF01420. Methylase_S. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07990-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRGKLPEGW ATAPVSTVTT LIRGVTYKKE QALNYLQDDY LPIIRANNIQ    50
    NGKFDTTDLV FVPKNLVKES QKISPEDIVI AMSSGSKSVV GKSAHQRLPF 100
    ECSFGAFCGA LRPEKFISPN YIAHFTKSSF YRNKISSLSA GANINNIKPA 150
    SFDLINIPIP SLAEQKIIAE KLDTLLAQVD STKARLEQIP QILKRFRQAV 200
    LAAAVSGTLT TALRNSHSLI GWHSTNLGAL IVDSCNGLAK RQGLNGNEIT 250
    ILRLADFKDA QRIIGNERKI KLDSKEENKY SLENDDILVI RVNGSADLAG 300
    RFIEYKSNGD IEGFCDHFIR LRLDSNKIMS RFLTYIANEG EGRFYLRNSL 350
    STSAGQNTIN QTSIKGLSFL LPPLKEQAEI VRRVEQLFAY ADTIEKQVNN 400
    ALTRVNSLTQ SILAKAFRGE LTAQWRAENP DLISGKNSAA ALLEKIKAER 450
    AVSGGKKTSR KKA 463
    Length:463
    Mass (Da):51,190
    Last modified:August 1, 1988 - v1
    Checksum:i1C42A1717F1E85CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14984 Genomic DNA. Translation: AAA27145.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14984 Genomic DNA. Translation: AAA27145.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3689. S.StySPI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR000055. Restrct_endonuc_typeI_HsdS.
    [Graphical view ]
    Pfami PF01420. Methylase_S. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two DNA recognition domains of the specificity polypeptides of a family of type I restriction enzymes."
      Fuller-Pace F.V., Murray N.E.
      Proc. Natl. Acad. Sci. U.S.A. 83:9368-9372(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiT1S_SALPO
    AccessioniPrimary (citable) accession number: P07990
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3