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P07989

- T1M_SALPO

UniProt

P07989 - T1M_SALPO

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Protein

Type I restriction enzyme StySPI M protein

Gene
hsdM, hsdT
Organism
Salmonella potsdam
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Methylation of specific adenine residues; required for both restriction and modification activities By similarity.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei216 – 2161S-adenosyl-L-methionine By similarity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3518. M.StySPI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type I restriction enzyme StySPI M protein (EC:2.1.1.72)
Short name:
M.StySPI
Gene namesi
Name:hsdM
Synonyms:hsdT
OrganismiSalmonella potsdam
Taxonomic identifieri597 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 529529Type I restriction enzyme StySPI M proteinPRO_0000088025Add
BLAST

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Structurei

3D structure databases

ProteinModelPortaliP07989.
SMRiP07989. Positions 4-527.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1536S-adenosyl-L-methionine binding By similarity
Regioni178 – 1803S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR022749. D12N6_MeTrfase_N.
IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF12161. HsdM_N. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07989-1 [UniParc]FASTAAdd to Basket

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MNNNDLVAKL WKLCDNLRDG GVSYQNYVNE LASLLFLKMC KETGQEADYL    50
PEGYRWDDLK SRIGQEQLQF YRNLLVHLGA DEKKLVQAVF QNVNTTITQP 100
KQLTELVSSM DSLDWYNGDH GKSRDDFGDM YEGLLQKNAN ETKSGAGQYF 150
TPRPLIKTII HLLKPQPREV VQDPAAGTAG FLIEADRYVK SQTNDLDDLD 200
GDAQDFQIKK AFVGLELVPG TRRLALMNCL LHDIEGNLDH GGAIRLGNTL 250
GSDGENLPQA DIVATNPPFG SAAGTNITRT FVHPTSNKQL CFMQHIIETL 300
PPGGRAAAVV PDNVLFEGGK GTDIRRDLMD KCHLHTILRL PTGIFYAQGV 350
KTNVLFFTKG TVANPNQDKN CTDDVWVYDL RTNMPSFGKR TPFTEQHLQP 400
FETVYGEDPH GLSPRTEGEW SFNAEESEVA DSEENKNADQ HQATSRWRKF 450
SREWIRTAKS DSLDISWLKD KDSIDADSLP EPDVLAAEAM GELVQALGEL 500
DALMRELGAG DEADAQRQLL EEAFGGVKA 529
Length:529
Mass (Da):58,906
Last modified:February 1, 1995 - v2
Checksum:i07895A9ADA4FA249
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L02507 Genomic DNA. Translation: AAA27143.1.
M14984 Genomic DNA. Translation: AAA27144.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L02507 Genomic DNA. Translation: AAA27143.1 .
M14984 Genomic DNA. Translation: AAA27144.1 .

3D structure databases

ProteinModelPortali P07989.
SMRi P07989. Positions 4-527.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3518. M.StySPI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR022749. D12N6_MeTrfase_N.
IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF12161. HsdM_N. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Roles of selection and recombination in the evolution of type I restriction-modification systems in enterobacteria."
    Sharp P.M., Kelleher J.E., Daniel A.S., Cowan G.M., Murray N.E.
    Proc. Natl. Acad. Sci. U.S.A. 89:9836-9840(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Two DNA recognition domains of the specificity polypeptides of a family of type I restriction enzymes."
    Fuller-Pace F.V., Murray N.E.
    Proc. Natl. Acad. Sci. U.S.A. 83:9368-9372(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 455-529.

Entry informationi

Entry nameiT1M_SALPO
AccessioniPrimary (citable) accession number: P07989
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg2+ as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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