ID   GUN1_BACSU              Reviewed;         499 AA.
AC   P07983;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 2.
DT   16-JUN-2009, entry version 69.
DE   RecName: Full=Endoglucanase;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta-glucanase;
DE   AltName: Full=Cellulase;
DE   Flags: Precursor;
GN   Name=bglC; Synonyms=gld;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DLG;
RX   MEDLINE=87194581; PubMed=3106328;
RA   Robson L.M., Chambliss G.H.;
RT   "Endo-beta-1,4-glucanase gene of Bacillus subtilis DLG.";
RL   J. Bacteriol. 169:2017-2025(1987).
CC   -!- CATALYTIC ACTIVITY: Endohydrolysis of (1->4)-beta-D-glucosidic
CC       linkages in cellulose, lichenin and cereal beta-D-glucans.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A)
CC       family.
CC   -!- SIMILARITY: Contains 1 CBM3 (carbohydrate binding type-3) domain.
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DR   EMBL; M16185; AAA22496.1; ALT_INIT; Genomic_DNA.
DR   PIR; A26874; A26874.
DR   HSSP; Q59232; 1LF1.
DR   SMR; P07983; 36-330.
DR   CAZy; CBM3; Carbohydrate-Binding Module Family 3.
DR   CAZy; GH5; Glycoside Hydrolase Family 5.
DR   BRENDA; 3.2.1.4; 150.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0043169; F:cation binding; IEA:InterPro.
DR   GO; GO:0008810; F:cellulase activity; IEA:EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001956; CBD_3.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR018087; Glyco_hydro_5_CS.
DR   InterPro; IPR013781; Glyco_hydro_sg_catalytic.
DR   Gene3D; G3DSA:2.60.40.710; CBD_3; 1.
DR   Gene3D; G3DSA:3.20.20.80; Glyco_hydro_cat; 1.
DR   Pfam; PF00942; CBM_3; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   ProDom; PD001947; CBD_3; 1.
DR   PROSITE; PS51172; CBM3; 1.
DR   PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cellulose degradation; Glycosidase;
KW   Hydrolase; Polysaccharide degradation; Signal.
FT   SIGNAL        1     29
FT   CHAIN        30    499       Endoglucanase.
FT                                /FTId=PRO_0000007839.
FT   DOMAIN      350    499       CBM3.
FT   ACT_SITE    169    169       Proton donor (By similarity).
FT   ACT_SITE    257    257       Nucleophile (By similarity).
SQ   SEQUENCE   499 AA;  55187 MW;  339D04EE95A63EE1 CRC64;
     MKRSISIFIT CLLIAVLTMG GLLPSPASAA GTKTPVAKNG QLSIKGTQLV NRDGKAVQLK
     GISSHGLQWY GDFVNKDSLK WLRDDWGITV FRAAMYTADG GYIDNPSVKN KVKEAVEAAK
     ELGIYVIIDW HILNDGNPNQ NKEKAKEFFK EMSSLYGNTP NVIYEIANEP NGDVNWKRDI
     KPYAEEVISV IRKNDPDNII IVGTGTWSQD VNDAADDQLK DANVMYALHF YAGTHGQSLR
     DKANYALSKG APIFVTEWGT SDASGNGGVF LDQSREWLNY LDSKNISWVN WNLSDKQESS
     SALKPGASKT GGWPLTDLTA SGTFVRENIR GTKDSTKDVP ETPAQDNPTQ EKGVSVQYKA
     GDGRVNSNQI RPQLHIKNNG NATVDLKDVT ARYWYNVKNK GQNFDCDYAQ MGCGNLTHKF
     VTLHKPKQGA DTYLELGFKT GTLSPGASTG NIQLRLHNDD WSNYAQSGDY SFFQSNTFKT
     TKKITLYHQG KLIWGTEPN
//