Reviewed, UniProtKB/Swiss-Prot P07980 (GUB_BACAM)

Names and origin

Protein names

Recommended name:
    Beta-glucanase
    EC=3.2.1.73
Alternative name(s):
    Endo-beta-1,3-1,4 glucanase
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Lichenase

Gene names

Name:

bglA

Organism

Bacillus amyloliquefaciens

Taxonomic identifier

1390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.
Miscellaneous	Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	licheninase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25
	Chain	26 – 239	214	Beta-glucanase		PRO_0000011786
Sites
	Active site	134	1	Nucleophile By similarity
Amino acid modifications
	Disulfide bond	57 ↔ 86		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07980-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: A76A64268A7AAA0B	FASTA	239	26,928
	10 20 30 40 50 60 MKRVLLILVT GLFMSLCGIT SSVSAQTGGS FFEPFNSYNS GLWQKADGYS NGDMFNCTWR 70 80 90 100 110 120 ANNVSMTSLG EMRLALTSPS YNKFDCGENR SVQTYGYGLY EVRMKPAKNT GIVSSFFTYT 130 140 150 160 170 180 GPTEGTPWDE IDIEFLGKDT TKVQFNYYTN GAGNHEKFAD LGFDAANAYH TYAFDWQPNS 190 200 210 220 230 IKWYVDGQLK HTATTQIPAA PGKIMMNLWN GTGVDDWLGS YNGVNPIYAH YDWMRYRKK

References

[1]	"The beta-glucanase gene from Bacillus amyloliquefaciens shows extensive homology with that of Bacillus subtilis." Hofemeister J., Kurtz A., Borriss R., Knowles J. Gene 49:177-187(1986) [PubMed: 3106158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BE 20/78.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M15674 Genomic DNA. Translation: AAA87323.1.
PIR	A29091.
3D structure databases
HSSP	HSSP built from PDB template 1GBG based on UniProtKB P27051.
SMR	P07980. Positions 26-239.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Enzyme and pathway databases
BRENDA	3.2.1.73. 1130.
Family and domain databases
InterPro	IPR008264. Beta_glucanase. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
PRINTS	PR00737. GLHYDRLASE16.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUB_BACAM

Accession

Primary (citable) accession number: P07980

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)