P07979 (GUB_NICPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lichenase
EC=3.2.1.73

Alternative name(s):
Endo-beta-1,3-1,4 glucanase

Gene names

Name:

GN1

Organism

Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)

Taxonomic identifier

4092 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.
Pathway	Glycan metabolism; beta-D-glucan degradation.
Miscellaneous	The expression of beta-glucanase is influenced by auxins and cytokinins.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	licheninase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 370	340	Lichenase		PRO_0000011898
Sites
	Active site	271	1	Nucleophile By similarity
	Active site	328	1	Proton donor By similarity
Experimental info
	Sequence conflict	164	1	S → T in AAA34078. Ref.2
	Sequence conflict	199	1	R → G in AAA34078. Ref.2
	Sequence conflict	250	1	S → L in AAA34078. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P07979 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: CB359DFD188F84C1	FASTA	370	40,599
	10 20 30 40 50 60 MDTSHKHIAL QMAAIILLGL LVSSTEIVGA QSVGVCYGML GNNLPPASQV VQLYKSKNIR 70 80 90 100 110 120 RMRLYDPNQA ALQALRGSNI EVMLGVPNSD LQNIAANPSN ANNWVQRNVR NFWPAVKFRY 130 140 150 160 170 180 IAVGNEVSPV TGTSSLTRYL LPAMRNIRNA ISSAGLQNNI KVSSSVDMTL IGNSFPPSQG 190 200 210 220 230 240 SFRNDVRSFI DPIIGFVRRI NSPLLVNIYP YFSYAGNPRD ISLPYALFTA PNVVVQDGSL 250 260 270 280 290 300 GYRNLFDAMS DAVYAALSRA GGGSIEIVVS ESGWPSAGAF AATTNNAATY YKNLIQHVKR 310 320 330 340 350 360 GSPRRPNKVI ETYLFAMFDE NNKNPELEKH FGLFSPNKQP KYPLSFGFSD RYWDISAENN 370 ATAASLISEM

References

[1]	"Primary structure of a hormonally regulated beta-glucanase of Nicotiana plumbaginifolia." de Loose M., Alliotte T., Gheysen G., Genetello C., Gielen J., Soetaert P., van Montagu M., Inze D. Gene 70:13-23(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. P2.
[2]	"Tissue-specific and pathogen-induced regulation of a Nicotiana plumbaginifolia beta-1,3-glucanase gene." Castresana C., de Carvalho F., Gheysen G., Habets M., Inze D., van Montagu M. Plant Cell 2:1131-1143(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07280 mRNA. Translation: CAA30261.1. M63634 Genomic DNA. Translation: AAA34078.1.
PIR	JQ0982. S03209.
3D structure databases
ProteinModelPortal	P07979.
SMR	P07979. Positions 33-346.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00350.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUB_NICPL

Accession

Primary (citable) accession number: P07979

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program