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Protein

Lichenase

Gene

GN1

Organism
Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Pathway: beta-D-glucan degradation

This protein is involved in the pathway beta-D-glucan degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway beta-D-glucan degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei271 – 2711NucleophileBy similarity
Active sitei328 – 3281Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

UniPathwayiUPA00350.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Lichenase (EC:3.2.1.73)
Alternative name(s):
Endo-beta-1,3-1,4 glucanase
Gene namesi
Name:GN1
OrganismiNicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Taxonomic identifieri4092 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 370340LichenasePRO_0000011898Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP07979.
SMRiP07979. Positions 33-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTSHKHIAL QMAAIILLGL LVSSTEIVGA QSVGVCYGML GNNLPPASQV
60 70 80 90 100
VQLYKSKNIR RMRLYDPNQA ALQALRGSNI EVMLGVPNSD LQNIAANPSN
110 120 130 140 150
ANNWVQRNVR NFWPAVKFRY IAVGNEVSPV TGTSSLTRYL LPAMRNIRNA
160 170 180 190 200
ISSAGLQNNI KVSSSVDMTL IGNSFPPSQG SFRNDVRSFI DPIIGFVRRI
210 220 230 240 250
NSPLLVNIYP YFSYAGNPRD ISLPYALFTA PNVVVQDGSL GYRNLFDAMS
260 270 280 290 300
DAVYAALSRA GGGSIEIVVS ESGWPSAGAF AATTNNAATY YKNLIQHVKR
310 320 330 340 350
GSPRRPNKVI ETYLFAMFDE NNKNPELEKH FGLFSPNKQP KYPLSFGFSD
360 370
RYWDISAENN ATAASLISEM
Length:370
Mass (Da):40,599
Last modified:November 1, 1997 - v3
Checksum:iCB359DFD188F84C1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641S → T in AAA34078 (PubMed:2152158).Curated
Sequence conflicti199 – 1991R → G in AAA34078 (PubMed:2152158).Curated
Sequence conflicti250 – 2501S → L in AAA34078 (PubMed:2152158).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07280 mRNA. Translation: CAA30261.1.
M63634 Genomic DNA. Translation: AAA34078.1.
PIRiJQ0982.
S03209.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07280 mRNA. Translation: CAA30261.1.
M63634 Genomic DNA. Translation: AAA34078.1.
PIRiJQ0982.
S03209.

3D structure databases

ProteinModelPortaliP07979.
SMRiP07979. Positions 33-346.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00350.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of a hormonally regulated beta-glucanase of Nicotiana plumbaginifolia."
    de Loose M., Alliotte T., Gheysen G., Genetello C., Gielen J., Soetaert P., van Montagu M., Inze D.
    Gene 70:13-23(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. P2.
  2. "Tissue-specific and pathogen-induced regulation of a Nicotiana plumbaginifolia beta-1,3-glucanase gene."
    Castresana C., de Carvalho F., Gheysen G., Habets M., Inze D., van Montagu M.
    Plant Cell 2:1131-1143(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGUB_NICPL
AccessioniPrimary (citable) accession number: P07979
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1997
Last modified: October 1, 2014
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The expression of beta-glucanase is influenced by auxins and cytokinins.

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.