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P07971

- HEMA_INCGL

UniProt

P07971 - HEMA_INCGL

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Protein

Hemagglutinin-esterase-fusion glycoprotein

Gene

HE

Organism
Influenza C virus (strain C/Great lakes/1167/1954)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell (By similarity).By similarity

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei58 – 581NucleophileBy similarity
Active sitei353 – 3531Charge relay systemBy similarity
Active sitei356 – 3561Charge relay systemBy similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. fusion of virus membrane with host plasma membrane Source: InterPro
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase-fusion glycoprotein (EC:3.1.1.53)
Short name:
HEF
Cleaved into the following 2 chains:
Gene namesi
Name:HE
OrganismiInfluenza C virus (strain C/Great lakes/1167/1954)
Taxonomic identifieri11557 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus C
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 1›1
Chaini2 – 433432Hemagglutinin-esterase-fusion glycoprotein chain 1PRO_0000039148Add
BLAST
Chaini434 – 642209Hemagglutinin-esterase-fusion glycoprotein chain 2PRO_0000039149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi7 ↔ 570Interchain (between HEF1 and HEF2 chains)By similarity
Glycosylationi13 – 131N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi48 – 481N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi107 ↔ 152By similarity
Disulfide bondi127 ↔ 175By similarity
Glycosylationi131 – 1311N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi197 ↔ 239By similarity
Disulfide bondi216 ↔ 303By similarity
Disulfide bondi224 ↔ 276By similarity
Disulfide bondi333 ↔ 339By similarity
Glycosylationi382 – 3821N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HEF1-HEF2.By similarity

Structurei

3D structure databases

ProteinModelPortaliP07971.
SMRiP07971. Positions 2-428, 438-598.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 617616ExtracellularSequence AnalysisAdd
BLAST
Topological domaini639 – 6424CytoplasmicSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei618 – 63821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 2726Fusion domain-1By similarityAdd
BLAST
Regioni28 – 138111Esterase domain-1By similarityAdd
BLAST
Regioni138 – 297160N-acetyl-9-O-acetylneuraminic acid bindingBy similarityAdd
BLAST
Regioni298 – 35255Esterase domain-2By similarityAdd
BLAST
Regioni353 – 638286Fusion domain-2By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.20.10. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07971 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
AEKIKICLQK QANSSFSLHN GFGGNLYATE EKRMFELVKP KAGASVLNQS
60 70 80 90 100
TWIGFGDSRT DKSNSAFPRS ADVSAKTADK FRSLSGGSLM LSMFGPPGKV
110 120 130 140 150
DYLYQGCGKH KVFYEGVNWS PHAAIDCYRK NWTDIKLNFQ KNIYELASQS
160 170 180 190 200
HCMSLVNALD KTIPLQVTAE VAKNCNNSFL KNPALYTQEV NPSKQICGEE
210 220 230 240 250
NLAFFTLPTQ FGTYECKLHL VASCYFIYDS KEVYNKRGCD NYFQVIYDSS
260 270 280 290 300
GKVVGGLDNR VSPYTGNTGD TPTMQCDMLQ LKPGRYSVRS SPRFLLMPER
310 320 330 340 350
SYCFDMKEKG LVTAVQSVWG KGRKSDYAVD QACLSTPGCM LIQKQKPYIG
360 370 380 390 400
EADDHHGDQE MRELLSGLDY EARCISQSGW VNETSPFTEE YLLPPKFGRC
410 420 430 440 450
PLAAKEESIP KIPDGLLIPT SGTDTTVTKP KSRIFGNDDL IIGLLFVAIV
460 470 480 490 500
ETGIGGYLLG SRKESGGGVT KESAEKGFEK IGNDIQILRS STNIAIEKLN
510 520 530 540 550
DRITHDEQAI RDLTLEIENA RSEALLGELG IIRALLVGNI SIGLQESLWE
560 570 580 590 600
LASEITNRAG DLAVEVSPGC WIIDNNICDQ SCQNFIFKFN ETAPVPTIPP
610 620 630 640
LDTKIDLQSD PFYWGSSLGL AITTPISLAA LAISGIAICR TK
Length:642
Mass (Da):70,704
Last modified:August 1, 1988 - v1
Checksum:i2A5C80CB229C48C1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11639 Genomic RNA. Translation: AAA43784.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11639 Genomic RNA. Translation: AAA43784.1 .

3D structure databases

ProteinModelPortali P07971.
SMRi P07971. Positions 2-428, 438-598.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.90.20.10. 1 hit.
InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Noncumulative sequence changes in the hemagglutinin genes of influenza C virus isolates."
    Buonagurio D.A., Nakada S., Desselberger U., Krystal M., Palese P.
    Virology 146:221-232(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_INCGL
AccessioniPrimary (citable) accession number: P07971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3