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P07970

- HEMA_INCMI

UniProt

P07970 - HEMA_INCMI

Protein

Hemagglutinin-esterase-fusion glycoprotein

Gene

HE

Organism
Influenza C virus (strain C/Mississippi/1980)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell By similarity.By similarity

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei58 – 581NucleophileBy similarity
    Active sitei352 – 3521Charge relay systemBy similarity
    Active sitei355 – 3551Charge relay systemBy similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
    2. fusion of virus membrane with host plasma membrane Source: InterPro
    3. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Keywords - Biological processi

    Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase-fusion glycoprotein (EC:3.1.1.53)
    Short name:
    HEF
    Cleaved into the following 2 chains:
    Gene namesi
    Name:HE
    OrganismiInfluenza C virus (strain C/Mississippi/1980)
    Taxonomic identifieri203229 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus C
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei‹1 – 1›1
    Chaini2 – 432431Hemagglutinin-esterase-fusion glycoprotein chain 1PRO_0000039156Add
    BLAST
    Chaini433 – 641209Hemagglutinin-esterase-fusion glycoprotein chain 2PRO_0000039157Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi7 ↔ 569Interchain (between HEF1 and HEF2 chains)By similarity
    Glycosylationi13 – 131N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi48 – 481N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi107 ↔ 152By similarity
    Disulfide bondi127 ↔ 175By similarity
    Glycosylationi131 – 1311N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi196 ↔ 238By similarity
    Disulfide bondi215 ↔ 302By similarity
    Disulfide bondi223 ↔ 275By similarity
    Disulfide bondi332 ↔ 338By similarity
    Glycosylationi381 – 3811N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homotrimer of disulfide-linked HEF1-HEF2.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP07970.
    SMRiP07970. Positions 2-427, 436-597.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 616615ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini638 – 6414CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei617 – 63721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 2726Fusion domain-1By similarityAdd
    BLAST
    Regioni28 – 138111Esterase domain-1By similarityAdd
    BLAST
    Regioni138 – 296159N-acetyl-9-O-acetylneuraminic acid bindingBy similarityAdd
    BLAST
    Regioni297 – 35155Esterase domain-2By similarityAdd
    BLAST
    Regioni352 – 637286Fusion domain-2By similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di3.90.20.10. 1 hit.
    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    IPR013829. Hemagglutn_stalk.
    IPR014831. Hemagglutn_stalk_influenz-C.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    PF08720. Hema_stalk. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07970-1 [UniParc]FASTAAdd to Basket

    « Hide

    AEKIKICLQK QVNSSFSLHN GFGGNLYATE EKRMFELVKP KAGASVLNQS    50
    TWIGFGDSRT DKSNPNFPRS ADVSVKTANK FRSLTGGSLM LSMFGPPGKV 100
    DYLYQGCGKH KVFYEGVNWS PHAAIDCYRK NWTDIKLNFQ KNIYELASQS 150
    HCMSLVNALD KTIPLQATAG VAGNCNNSFL KNPALYTQEV TPPEKCGKEN 200
    LAFFTLPTQF GTYECKLHLV ASCYFIYDSK EVYNKRGCDN YFQVIYDSSG 250
    KVVGGLDNRV SPYTGNTGDT PTMQCDMLQL KPGRYSVRSS PRFLLMPERS 300
    YCFDMKEKGL VTAVQSIWGK DRKSDYAVDQ ACLSTPGCML IQKQKPYTGE 350
    ADDHHGDQEM RELLSGLDYE ARCISQSGWV NETSPFTEEY LLPPKFGRCP 400
    LAAKEESIPK IPDGLLIPTS GTDTTVTKPK SRIFGIDDLI IGLLFVAIVE 450
    AGIGGYLLGS RKESGGGVTK ESAEKGFEKI GNDIQILRSS TNIAIEKLND 500
    RITHDEQAIR DLTLEIENAR SEALLGELGI IRALLVGNIS IGLQESLWEL 550
    ASEITNRAGD LAVEISPGCW IIDNNICDQS CQNFIFKFNE TAPVPTIPPL 600
    DTKIDLQSDP FYWGSSLGLA ITTPISLAAL VISGIAICRT K 641
    Length:641
    Mass (Da):70,610
    Last modified:August 1, 1988 - v1
    Checksum:iA86446DB393A36B3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11640 Genomic RNA. Translation: AAA43785.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11640 Genomic RNA. Translation: AAA43785.1 .

    3D structure databases

    ProteinModelPortali P07970.
    SMRi P07970. Positions 2-427, 436-597.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.90.20.10. 1 hit.
    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    IPR013829. Hemagglutn_stalk.
    IPR014831. Hemagglutn_stalk_influenz-C.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    PF08720. Hema_stalk. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Noncumulative sequence changes in the hemagglutinin genes of influenza C virus isolates."
      Buonagurio D.A., Nakada S., Desselberger U., Krystal M., Palese P.
      Virology 146:221-232(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiHEMA_INCMI
    AccessioniPrimary (citable) accession number: P07970
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3