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P07970

- HEMA_INCMI

UniProt

P07970 - HEMA_INCMI

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Protein

Hemagglutinin-esterase-fusion glycoprotein

Gene
HE
Organism
Influenza C virus (strain C/Mississippi/1980)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell By similarity.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei58 – 581Nucleophile By similarity
Active sitei352 – 3521Charge relay system By similarity
Active sitei355 – 3551Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. fusion of virus membrane with host plasma membrane Source: InterPro
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase-fusion glycoprotein (EC:3.1.1.53)
Short name:
HEF
Cleaved into the following 2 chains:
Gene namesi
Name:HE
OrganismiInfluenza C virus (strain C/Mississippi/1980)
Taxonomic identifieri203229 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus C
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 616615Extracellular Reviewed predictionAdd
BLAST
Transmembranei617 – 63721Helical; Reviewed predictionAdd
BLAST
Topological domaini638 – 6414Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 1›1
Chaini2 – 432431Hemagglutinin-esterase-fusion glycoprotein chain 1PRO_0000039156Add
BLAST
Chaini433 – 641209Hemagglutinin-esterase-fusion glycoprotein chain 2PRO_0000039157Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi7 ↔ 569Interchain (between HEF1 and HEF2 chains) By similarity
Glycosylationi13 – 131N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi48 – 481N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi107 ↔ 152 By similarity
Disulfide bondi127 ↔ 175 By similarity
Glycosylationi131 – 1311N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi196 ↔ 238 By similarity
Disulfide bondi215 ↔ 302 By similarity
Disulfide bondi223 ↔ 275 By similarity
Disulfide bondi332 ↔ 338 By similarity
Glycosylationi381 – 3811N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HEF1-HEF2 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP07970.
SMRiP07970. Positions 2-427, 436-597.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 2726Fusion domain-1 By similarityAdd
BLAST
Regioni28 – 138111Esterase domain-1 By similarityAdd
BLAST
Regioni138 – 296159N-acetyl-9-O-acetylneuraminic acid binding By similarityAdd
BLAST
Regioni297 – 35155Esterase domain-2 By similarityAdd
BLAST
Regioni352 – 637286Fusion domain-2 By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.20.10. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07970-1 [UniParc]FASTAAdd to Basket

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AEKIKICLQK QVNSSFSLHN GFGGNLYATE EKRMFELVKP KAGASVLNQS    50
TWIGFGDSRT DKSNPNFPRS ADVSVKTANK FRSLTGGSLM LSMFGPPGKV 100
DYLYQGCGKH KVFYEGVNWS PHAAIDCYRK NWTDIKLNFQ KNIYELASQS 150
HCMSLVNALD KTIPLQATAG VAGNCNNSFL KNPALYTQEV TPPEKCGKEN 200
LAFFTLPTQF GTYECKLHLV ASCYFIYDSK EVYNKRGCDN YFQVIYDSSG 250
KVVGGLDNRV SPYTGNTGDT PTMQCDMLQL KPGRYSVRSS PRFLLMPERS 300
YCFDMKEKGL VTAVQSIWGK DRKSDYAVDQ ACLSTPGCML IQKQKPYTGE 350
ADDHHGDQEM RELLSGLDYE ARCISQSGWV NETSPFTEEY LLPPKFGRCP 400
LAAKEESIPK IPDGLLIPTS GTDTTVTKPK SRIFGIDDLI IGLLFVAIVE 450
AGIGGYLLGS RKESGGGVTK ESAEKGFEKI GNDIQILRSS TNIAIEKLND 500
RITHDEQAIR DLTLEIENAR SEALLGELGI IRALLVGNIS IGLQESLWEL 550
ASEITNRAGD LAVEISPGCW IIDNNICDQS CQNFIFKFNE TAPVPTIPPL 600
DTKIDLQSDP FYWGSSLGLA ITTPISLAAL VISGIAICRT K 641
Length:641
Mass (Da):70,610
Last modified:August 1, 1988 - v1
Checksum:iA86446DB393A36B3
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11640 Genomic RNA. Translation: AAA43785.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11640 Genomic RNA. Translation: AAA43785.1 .

3D structure databases

ProteinModelPortali P07970.
SMRi P07970. Positions 2-427, 436-597.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.90.20.10. 1 hit.
InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Noncumulative sequence changes in the hemagglutinin genes of influenza C virus isolates."
    Buonagurio D.A., Nakada S., Desselberger U., Krystal M., Palese P.
    Virology 146:221-232(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_INCMI
AccessioniPrimary (citable) accession number: P07970
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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