HE - Hemagglutinin-esterase-fusion glycoprotein precursor - Influenza C virus (strain C/Pig/Beijing/115/1981)

Functionⁱ

Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell.UniRule annotation

Catalytic activityⁱ

N-acetyl-O-acetylneuraminate + H₂O = N-acetylneuraminate + acetate.UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	353	Charge relay systemUniRule annotation		1
Active siteⁱ	356	Charge relay systemUniRule annotation		1

GO - Molecular functionⁱ

host cell surface receptor binding Source: InterPro
sialate O-acetylesterase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

endocytosis involved in viral entry into host cell Source: UniProtKB-KW
fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
fusion of virus membrane with host plasma membrane Source: InterPro
virion attachment to host cell Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hemagglutinin, Hydrolase
Biological process	Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Hemagglutinin-esterase-fusion glycoproteinUniRule annotation (EC:3.1.1.53UniRule annotation) Short name: HEFUniRule annotation Cleaved into the following 2 chains: Hemagglutinin-esterase-fusion glycoprotein chain 1UniRule annotation Short name: HEF1UniRule annotation Hemagglutinin-esterase-fusion glycoprotein chain 2UniRule annotation Short name: HEF2UniRule annotation
Gene namesⁱ	Name:HEUniRule annotation
Organismⁱ	Influenza C virus (strain C/Pig/Beijing/115/1981)
Taxonomic identifierⁱ	203231 [NCBI]
Taxonomic lineageⁱ	Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus C › Influenza C virus
Virus hostⁱ	Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationⁱ

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 617	ExtracellularUniRule annotationAdd BLAST	617
Transmembraneⁱ	618 – 638	HelicalUniRule annotationAdd BLAST	21
Topological domainⁱ	639 – 642	CytoplasmicUniRule annotation	4

GO - Cellular componentⁱ

host cell plasma membrane Source: UniProtKB-SubCell
integral component of membrane Source: UniProtKB-KW
viral envelope Source: UniProtKB-KW
virion membrane Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000440765	1 – 433	Hemagglutinin-esterase-fusion glycoprotein chain 1UniRule annotationAdd BLAST		433
ChainⁱPRO_0000039165	434 – 642	Hemagglutinin-esterase-fusion glycoprotein chain 2UniRule annotationAdd BLAST		209

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	13	N-linked (GlcNAc...) asparagine; by hostUniRule annotation	1
Glycosylationⁱ	48	N-linked (GlcNAc...) asparagine; by hostUniRule annotation	1
Glycosylationⁱ	131	N-linked (GlcNAc...) asparagine; by hostUniRule annotation	1
Glycosylationⁱ	176	N-linked (GlcNAc...) asparagine; by hostUniRule annotation	1
Disulfide bondⁱ	197 ↔ 239	UniRule annotation
Disulfide bondⁱ	216 ↔ 303	UniRule annotation
Disulfide bondⁱ	224 ↔ 276	UniRule annotation
Glycosylationⁱ	382	N-linked (GlcNAc...) asparagine; by hostUniRule annotation	1
Glycosylationⁱ	539	N-linked (GlcNAc...) asparagine; by hostUniRule annotation	1
Glycosylationⁱ	590	N-linked (GlcNAc...) asparagine; by hostUniRule annotation	1

Post-translational modificationⁱ

In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease.UniRule annotation

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Interactionⁱ

Subunit structureⁱ

Homotrimer of disulfide-linked HEF1-HEF2.UniRule annotation

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P07967.
SMRⁱ	P07967.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	83 – 297	N-acetyl-9-O-acetylneuraminic acid bindingUniRule annotationAdd BLAST	215
Regionⁱ	297 – 351	Esterase domain-2UniRule annotationAdd BLAST	55
Regionⁱ	352 – 637	Fusion domain-2UniRule annotationAdd BLAST	286

Sequence similaritiesⁱ

Belongs to the influenza viruses hemagglutinin family.UniRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Family and domain databases

HAMAPⁱ	MF_04072. INFV_HEMA. 1 hit.
InterProⁱ	View protein in InterPro IPR008980. Capsid_hemagglutn. IPR007142. Hemagglutn-estrase_core. IPR003860. Hemagglutn-estrase_hemagglutn. IPR001364. Hemagglutn_influenz_A/B. IPR014831. Hemagglutn_stalk_influenz-C.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03996. Hema_esterase. 1 hit. PF02710. Hema_HEFG. 1 hit. PF08720. Hema_stalk. 1 hit.
SUPFAMⁱ	SSF49818. SSF49818. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Fragment.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P07967-1 [UniParc]FASTA Add to basket

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        10         20         30         40         50
AEKIKICLQK QVNSSFSLHN GFGGNLYATE EKRMFELVKP KAGASVLNQS 
        60         70         80         90        100
TWICFGDSRT DQSNSAFPRS ADVSAKTAEK FRSLSGGSLM LSMFGPPGKV 
       110        120        130        140        150
DYLYQGCGKH KVFYEGVNWS PHTAIDCYRK NWTDIKLNFQ KSIYELASQS 
       160        170        180        190        200
HCMSLVNALD KTIPLQATKG VAKNCNNSFL KNPALYTQEV KPLEQICGEE 
       210        220        230        240        250
NLAFFTLPTQ FGTYECKLHL VASCYFIYDS KEVYNKRGCG NYFQVIYDSS 
       260        270        280        290        300
GKVVGGLDNR VSPYTGNSGD TPTMQCDMLQ LKPGRYSVRS SPRFLLMPER 
       310        320        330        340        350
SYCFDMKEKG LVTAVQSIWG KGRKSDYAVD QACLSTPGCM LIQKQKPYIG 
       360        370        380        390        400
EADDHHGDQE MRELLSGLDY EARCISQSGW VNETSPFTEE YLLPPKFGRC 
       410        420        430        440        450
PLAAKEESIP KIPDGLLIPT SGTDTTVTKP KSRIFGIDDL IIGLLFVAIV 
       460        470        480        490        500
EAGIGGYLLG SRKESGGGVT KESAEKGFEK IGNDIQILRS STNIAIEKLN 
       510        520        530        540        550
DRISHDEQAI RDLTLEIENA RSEALLGELG IIRALLVGNI SIGLQESLWE 
       560        570        580        590        600
LASEITNRAG DLAVEVSPGC WIIDNNICDQ SCQNFIFKFN ETAPVPTIPP 
       610        620        630        640 
LDTKIDLQSD PFYWGSSLGL AITTPISLAA LVISGIAICR TK

Length:642

Mass (Da):70,719

Last modified:August 1, 1988 - v1

Checksum:ⁱ4F4CFA6E9D71EAC0

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Non-terminal residueⁱ	1			1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M11644 Genomic RNA. Translation: AAA43793.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

M11644 Genomic RNA. Translation: AAA43793.1.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P07967	P07975 UPI00001116B4 UPI00001116B3 P68762 A0A223FLF1 A0A223FKR9 A0A223FKW2 A0A223FKX3 A0A0E4AX34 A0A0E4AX33 +296	Influenza C virus (strain C/Johannesburg/1/1966) Influenza C virus (strain C/Ann Arbor/1/1950) Influenza C virus (C/Minnesota/24/2015) Influenza C virus (C/Minnesota/25/2015) Influenza C virus (C/Minnesota/26/2015) Influenza C virus (C/Minnesota/03/2014) Influenza C virus (C/Yamagata/35/2014) Influenza C virus (C/Yamagata/14/2014) Influenza C virus (C/Yamagata/13/2014) Influenza C virus (C/Miyagi/2/2014) And more	655	UniRef90_P07975	Cluster: Hemagglutinin-esterase-fusion glycoprotein	307

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P07967	P07975 UPI00001116B4 UPI00001116B3 A0A223FKV0 A0A223FLF1 P68762 A0A223FKN3 E0WL39 A0A223FKR0 A0A223FLG6 +343	Influenza C virus (strain C/Johannesburg/1/1966) Influenza C virus (C/Minnesota/33/2015) Influenza C virus (C/Minnesota/24/2015) Influenza C virus (strain C/Ann Arbor/1/1950) Influenza C virus (C/Minnesota/08/2015) Influenza C virus (strain C/JJ/1950) Influenza C virus (C/Minnesota/21/2015) Influenza C virus (C/Minnesota/31/2015) Influenza C virus (C/Minnesota/30/2015) Influenza C virus (C/Minnesota/04/2014) And more	655	UniRef50_P07975	Cluster: Hemagglutinin-esterase-fusion glycoprotein	354

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M11644 Genomic RNA. Translation: AAA43793.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

M11644 Genomic RNA. Translation: AAA43793.1.

3D structure databases

ProteinModelPortalⁱ	P07967.
SMRⁱ	P07967.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Family and domain databases

HAMAPⁱ	MF_04072. INFV_HEMA. 1 hit.
InterProⁱ	View protein in InterPro IPR008980. Capsid_hemagglutn. IPR007142. Hemagglutn-estrase_core. IPR003860. Hemagglutn-estrase_hemagglutn. IPR001364. Hemagglutn_influenz_A/B. IPR014831. Hemagglutn_stalk_influenz-C.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03996. Hema_esterase. 1 hit. PF02710. Hema_HEFG. 1 hit. PF08720. Hema_stalk. 1 hit.
SUPFAMⁱ	SSF49818. SSF49818. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HEMA_INCP2
Accessionⁱ	P07967Primary (citable) accession number: P07967
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
	Last sequence update:	August 1, 1988
	Last modified:	February 28, 2018
	This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB - P07967 (HEMA_INCP2)

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Hemagglutinin-esterase-fusion glycoprotein

HE

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Functionⁱ

Catalytic activityⁱ

Sites

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Topology

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Molecule processing

Amino acid modifications

Post-translational modificationⁱ

Keywords - PTMⁱ

Interactionⁱ

Subunit structureⁱ

Structureⁱ

3D structure databases

Family & Domainsⁱ

Region

Sequence similaritiesⁱ

Keywords - Domainⁱ

Family and domain databases

Sequenceⁱ

Experimental Info

Sequence databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

Entry informationⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P07967 (HEMA_INCP2)

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Hemagglutinin-esterase-fusion glycoprotein

HE

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Experimental Info

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ