UniProtKB - P07967 (HEMA_INCP2)
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Protein
Hemagglutinin-esterase-fusion glycoprotein
Gene
HE
Organism
Influenza C virus (strain C/Pig/Beijing/115/1981)
Status
Functioni
Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell.UniRule annotation
Catalytic activityi
N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 353 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 356 | Charge relay systemUniRule annotation | 1 |
GO - Molecular functioni
- host cell surface receptor binding Source: InterPro
- sialate O-acetylesterase activity Source: UniProtKB-EC
GO - Biological processi
- endocytosis involved in viral entry into host cell Source: UniProtKB-KW
- fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
- fusion of virus membrane with host plasma membrane Source: InterPro
- virion attachment to host cell Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Hemagglutinin-esterase-fusion glycoproteinUniRule annotation (EC:3.1.1.53UniRule annotation)Short name: HEFUniRule annotation Cleaved into the following 2 chains: Hemagglutinin-esterase-fusion glycoprotein chain 1UniRule annotation Short name: HEF1UniRule annotation Hemagglutinin-esterase-fusion glycoprotein chain 2UniRule annotation Short name: HEF2UniRule annotation |
Gene namesi | Name:HEUniRule annotation |
Organismi | Influenza C virus (strain C/Pig/Beijing/115/1981) |
Taxonomic identifieri | 203231 [NCBI] |
Taxonomic lineagei | Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus C › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823] |
Subcellular locationi
- Virion membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
- Host cell membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 617 | ExtracellularUniRule annotationAdd BLAST | 617 | |
Transmembranei | 618 – 638 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 639 – 642 | CytoplasmicUniRule annotation | 4 |
GO - Cellular componenti
- host cell plasma membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
- viral envelope Source: UniProtKB-KW
- virion membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host cell membrane, Host membrane, Membrane, Viral envelope protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000440765 | 1 – 433 | Hemagglutinin-esterase-fusion glycoprotein chain 1UniRule annotationAdd BLAST | 433 | |
ChainiPRO_0000039165 | 434 – 642 | Hemagglutinin-esterase-fusion glycoprotein chain 2UniRule annotationAdd BLAST | 209 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 13 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Glycosylationi | 48 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Glycosylationi | 131 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Glycosylationi | 176 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Disulfide bondi | 197 ↔ 239 | UniRule annotation | ||
Disulfide bondi | 216 ↔ 303 | UniRule annotation | ||
Disulfide bondi | 224 ↔ 276 | UniRule annotation | ||
Glycosylationi | 382 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Glycosylationi | 539 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Glycosylationi | 590 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 |
Post-translational modificationi
In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease.UniRule annotation
Keywords - PTMi
Disulfide bond, GlycoproteinInteractioni
Subunit structurei
Homotrimer of disulfide-linked HEF1-HEF2.UniRule annotation
Structurei
3D structure databases
ProteinModelPortali | P07967. |
SMRi | P07967. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 83 – 297 | N-acetyl-9-O-acetylneuraminic acid bindingUniRule annotationAdd BLAST | 215 | |
Regioni | 297 – 351 | Esterase domain-2UniRule annotationAdd BLAST | 55 | |
Regioni | 352 – 637 | Fusion domain-2UniRule annotationAdd BLAST | 286 |
Sequence similaritiesi
Belongs to the influenza viruses hemagglutinin family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
HAMAPi | MF_04072. INFV_HEMA. 1 hit. |
InterProi | View protein in InterPro IPR008980. Capsid_hemagglutn. IPR007142. Hemagglutn-estrase_core. IPR003860. Hemagglutn-estrase_hemagglutn. IPR001364. Hemagglutn_influenz_A/B. IPR014831. Hemagglutn_stalk_influenz-C. |
Pfami | View protein in Pfam PF03996. Hema_esterase. 1 hit. PF02710. Hema_HEFG. 1 hit. PF08720. Hema_stalk. 1 hit. |
SUPFAMi | SSF49818. SSF49818. 1 hit. |
i Sequence
Sequence statusi: Fragment.
: The displayed sequence is further processed into a mature form. Sequence processingi
P07967-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
AEKIKICLQK QVNSSFSLHN GFGGNLYATE EKRMFELVKP KAGASVLNQS
60 70 80 90 100
TWICFGDSRT DQSNSAFPRS ADVSAKTAEK FRSLSGGSLM LSMFGPPGKV
110 120 130 140 150
DYLYQGCGKH KVFYEGVNWS PHTAIDCYRK NWTDIKLNFQ KSIYELASQS
160 170 180 190 200
HCMSLVNALD KTIPLQATKG VAKNCNNSFL KNPALYTQEV KPLEQICGEE
210 220 230 240 250
NLAFFTLPTQ FGTYECKLHL VASCYFIYDS KEVYNKRGCG NYFQVIYDSS
260 270 280 290 300
GKVVGGLDNR VSPYTGNSGD TPTMQCDMLQ LKPGRYSVRS SPRFLLMPER
310 320 330 340 350
SYCFDMKEKG LVTAVQSIWG KGRKSDYAVD QACLSTPGCM LIQKQKPYIG
360 370 380 390 400
EADDHHGDQE MRELLSGLDY EARCISQSGW VNETSPFTEE YLLPPKFGRC
410 420 430 440 450
PLAAKEESIP KIPDGLLIPT SGTDTTVTKP KSRIFGIDDL IIGLLFVAIV
460 470 480 490 500
EAGIGGYLLG SRKESGGGVT KESAEKGFEK IGNDIQILRS STNIAIEKLN
510 520 530 540 550
DRISHDEQAI RDLTLEIENA RSEALLGELG IIRALLVGNI SIGLQESLWE
560 570 580 590 600
LASEITNRAG DLAVEVSPGC WIIDNNICDQ SCQNFIFKFN ETAPVPTIPP
610 620 630 640
LDTKIDLQSD PFYWGSSLGL AITTPISLAA LVISGIAICR TK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11644 Genomic RNA. Translation: AAA43793.1. |
Similar proteinsi
Entry informationi
Entry namei | HEMA_INCP2 | |
Accessioni | P07967Primary (citable) accession number: P07967 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | February 28, 2018 | |
This is version 102 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |