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Protein

Methyl-coenzyme M reductase subunit gamma

Gene

mcrG

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.

Catalytic activityi

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Pathwayi: methyl-coenzyme M reduction

This protein is involved in step 1 of the subpathway that synthesizes methane from methyl-coenzyme M.
Proteins known to be involved in this subpathway in this organism are:
  1. Methyl-coenzyme M reductase subunit gamma (mcrG), Methyl-coenzyme M reductase subunit beta (mcrB), Methyl-coenzyme M reductase subunit alpha (mcrA)
This subpathway is part of the pathway methyl-coenzyme M reduction, which is itself part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes methane from methyl-coenzyme M, the pathway methyl-coenzyme M reduction and in One-carbon metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Methanogenesis

Enzyme and pathway databases

BRENDAi2.8.4.1. 3250.
UniPathwayiUPA00646; UER00699.

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-coenzyme M reductase subunit gamma (EC:2.8.4.1)
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase gamma
Gene namesi
Name:mcrG
Ordered Locus Names:Mbar_A0894
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000008156 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001474732 – 248Methyl-coenzyme M reductase subunit gammaAdd BLAST247

Proteomic databases

PRIDEiP07964.

Interactioni

Subunit structurei

Hexamer of two alpha, two beta, and two gamma chains.1 Publication

Protein-protein interaction databases

STRINGi269797.Mbar_A0894.

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 23Combined sources11
Helixi34 – 41Combined sources8
Beta strandi52 – 54Combined sources3
Helixi57 – 60Combined sources4
Helixi66 – 70Combined sources5
Helixi75 – 79Combined sources5
Beta strandi83 – 90Combined sources8
Turni92 – 94Combined sources3
Helixi99 – 109Combined sources11
Beta strandi110 – 112Combined sources3
Beta strandi114 – 117Combined sources4
Beta strandi122 – 127Combined sources6
Helixi128 – 140Combined sources13
Turni146 – 148Combined sources3
Beta strandi149 – 151Combined sources3
Beta strandi176 – 179Combined sources4
Beta strandi182 – 187Combined sources6
Beta strandi193 – 199Combined sources7
Helixi206 – 212Combined sources7
Helixi223 – 225Combined sources3
Helixi227 – 245Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E6YX-ray1.60C/F2-248[»]
ProteinModelPortaliP07964.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07964.

Family & Domainsi

Phylogenomic databases

eggNOGiarCOG04858. Archaea.
COG4057. LUCA.
HOGENOMiHOG000225820.
KOiK00402.
OMAiKRTTIYR.

Family and domain databases

CDDicd00539. MCR_gamma. 1 hit.
Gene3Di3.90.320.20. 1 hit.
InterProiIPR009024. Me_CoM_Rdtase_Fd-like_fold.
IPR003178. Me_CoM_Rdtase_gsu.
[Graphical view]
PfamiPF02240. MCR_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDomiPD005845. Me_CoM_Rdtase_gsu. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55088. SSF55088. 1 hit.
TIGRFAMsiTIGR03259. met_CoM_red_gam. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07964-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYERQYYPG ATSVAANRRK HMSGKLEKLR EISDEDLTAV LGHRAPGSDY
60 70 80 90 100
PSTHPPLAEM GEPACSTREN VAATPGAAAG DRVRYIQFAD SMYNAPATPY
110 120 130 140 150
FRSYFAAINF RGVDPGTLSG RQIVEARERD MEQCAKVQME TEITDHALAG
160 170 180 190 200
VRGATVHGHS VRLQEDGVMF DMLDRRRLEN GTIIMDKDQV AIPLDRKVDL
210 220 230 240
GKPMSSEEAA KRTTIYRVDN VAFRDDAEVV EWVHRIFDQR TKFGFQPK
Length:248
Mass (Da):27,811
Last modified:January 23, 2007 - v3
Checksum:iAB23870B4BD7160B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00158 Genomic DNA. Translation: CAA68356.1.
CP000099 Genomic DNA. Translation: AAZ69868.1.
PIRiD29525.
RefSeqiWP_011305917.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ69868; AAZ69868; Mbar_A0894.
GeneIDi3625939.
KEGGimba:Mbar_A0894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00158 Genomic DNA. Translation: CAA68356.1.
CP000099 Genomic DNA. Translation: AAZ69868.1.
PIRiD29525.
RefSeqiWP_011305917.1. NC_007355.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E6YX-ray1.60C/F2-248[»]
ProteinModelPortaliP07964.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269797.Mbar_A0894.

Proteomic databases

PRIDEiP07964.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ69868; AAZ69868; Mbar_A0894.
GeneIDi3625939.
KEGGimba:Mbar_A0894.

Phylogenomic databases

eggNOGiarCOG04858. Archaea.
COG4057. LUCA.
HOGENOMiHOG000225820.
KOiK00402.
OMAiKRTTIYR.

Enzyme and pathway databases

UniPathwayiUPA00646; UER00699.
BRENDAi2.8.4.1. 3250.

Miscellaneous databases

EvolutionaryTraceiP07964.

Family and domain databases

CDDicd00539. MCR_gamma. 1 hit.
Gene3Di3.90.320.20. 1 hit.
InterProiIPR009024. Me_CoM_Rdtase_Fd-like_fold.
IPR003178. Me_CoM_Rdtase_gsu.
[Graphical view]
PfamiPF02240. MCR_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDomiPD005845. Me_CoM_Rdtase_gsu. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55088. SSF55088. 1 hit.
TIGRFAMsiTIGR03259. met_CoM_red_gam. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMCRG_METBF
AccessioniPrimary (citable) accession number: P07964
Secondary accession number(s): Q46E24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.