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P07964

- MCRG_METBF

UniProt

P07964 - MCRG_METBF

Protein

Methyl-coenzyme M reductase subunit gamma

Gene

mcrG

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.

    Catalytic activityi

    Methyl-CoM + CoB = CoM-S-S-CoB + methane.

    Pathwayi

    GO - Molecular functioni

    1. coenzyme-B sulfoethylthiotransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. methanogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Methanogenesis

    Enzyme and pathway databases

    BioCyciMBAR269797:GHUW-908-MONOMER.
    UniPathwayiUPA00646; UER00699.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methyl-coenzyme M reductase subunit gamma (EC:2.8.4.1)
    Alternative name(s):
    Coenzyme-B sulfoethylthiotransferase gamma
    Gene namesi
    Name:mcrG
    Ordered Locus Names:Mbar_A0894
    OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
    Taxonomic identifieri269797 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000008156: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 248247Methyl-coenzyme M reductase subunit gammaPRO_0000147473Add
    BLAST

    Proteomic databases

    PRIDEiP07964.

    Interactioni

    Subunit structurei

    Hexamer of two alpha, two beta, and two gamma chains.1 Publication

    Protein-protein interaction databases

    STRINGi269797.Mbar_A0894.

    Structurei

    Secondary structure

    1
    248
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi13 – 2311
    Helixi34 – 418
    Beta strandi52 – 543
    Helixi57 – 604
    Helixi66 – 705
    Helixi75 – 795
    Beta strandi83 – 908
    Turni92 – 943
    Helixi99 – 10911
    Beta strandi110 – 1123
    Beta strandi114 – 1174
    Beta strandi122 – 1276
    Helixi128 – 14013
    Turni146 – 1483
    Beta strandi149 – 1513
    Beta strandi176 – 1794
    Beta strandi182 – 1876
    Beta strandi193 – 1997
    Helixi206 – 2127
    Helixi223 – 2253
    Helixi227 – 24519

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E6YX-ray1.60C/F2-248[»]
    ProteinModelPortaliP07964.
    SMRiP07964. Positions 2-248.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07964.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG4057.
    HOGENOMiHOG000225820.
    KOiK00402.
    OMAiKRTTIYR.

    Family and domain databases

    Gene3Di3.90.320.20. 1 hit.
    InterProiIPR009024. Me_CoM_Rdtase_Fd-like_fold.
    IPR003178. Me_CoM_Rdtase_gsu.
    [Graphical view]
    PfamiPF02240. MCR_gamma. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000264. Meth_CoM_rd_gama. 1 hit.
    ProDomiPD005845. Me_CoM_Rdtase_gsu. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF55088. SSF55088. 1 hit.
    TIGRFAMsiTIGR03259. met_CoM_red_gam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07964-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYERQYYPG ATSVAANRRK HMSGKLEKLR EISDEDLTAV LGHRAPGSDY    50
    PSTHPPLAEM GEPACSTREN VAATPGAAAG DRVRYIQFAD SMYNAPATPY 100
    FRSYFAAINF RGVDPGTLSG RQIVEARERD MEQCAKVQME TEITDHALAG 150
    VRGATVHGHS VRLQEDGVMF DMLDRRRLEN GTIIMDKDQV AIPLDRKVDL 200
    GKPMSSEEAA KRTTIYRVDN VAFRDDAEVV EWVHRIFDQR TKFGFQPK 248
    Length:248
    Mass (Da):27,811
    Last modified:January 23, 2007 - v3
    Checksum:iAB23870B4BD7160B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00158 Genomic DNA. Translation: CAA68356.1.
    CP000099 Genomic DNA. Translation: AAZ69868.1.
    PIRiD29525.
    RefSeqiWP_011305917.1. NC_007355.1.
    YP_304448.1. NC_007355.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ69868; AAZ69868; Mbar_A0894.
    GeneIDi3625939.
    KEGGimba:Mbar_A0894.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00158 Genomic DNA. Translation: CAA68356.1 .
    CP000099 Genomic DNA. Translation: AAZ69868.1 .
    PIRi D29525.
    RefSeqi WP_011305917.1. NC_007355.1.
    YP_304448.1. NC_007355.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E6Y X-ray 1.60 C/F 2-248 [» ]
    ProteinModelPortali P07964.
    SMRi P07964. Positions 2-248.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269797.Mbar_A0894.

    Proteomic databases

    PRIDEi P07964.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ69868 ; AAZ69868 ; Mbar_A0894 .
    GeneIDi 3625939.
    KEGGi mba:Mbar_A0894.

    Phylogenomic databases

    eggNOGi COG4057.
    HOGENOMi HOG000225820.
    KOi K00402.
    OMAi KRTTIYR.

    Enzyme and pathway databases

    UniPathwayi UPA00646 ; UER00699 .
    BioCyci MBAR269797:GHUW-908-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P07964.

    Family and domain databases

    Gene3Di 3.90.320.20. 1 hit.
    InterProi IPR009024. Me_CoM_Rdtase_Fd-like_fold.
    IPR003178. Me_CoM_Rdtase_gsu.
    [Graphical view ]
    Pfami PF02240. MCR_gamma. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000264. Meth_CoM_rd_gama. 1 hit.
    ProDomi PD005845. Me_CoM_Rdtase_gsu. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF55088. SSF55088. 1 hit.
    TIGRFAMsi TIGR03259. met_CoM_red_gam. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the methyl coenzyme M reductase gene cluster from Methanosarcina barkeri."
      Bokranz M., Klein A.
      Nucleic Acids Res. 15:4350-4351(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
      Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
      J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Fusaro / DSM 804.
    3. "Comparison of three methyl-coenzyme M reductases from phylogenetically distant organisms: unusual amino acid modification, conservation and adaptation."
      Grabarse W., Mahlert F., Shima S., Thauer R.K., Ermler U.
      J. Mol. Biol. 303:329-344(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), SUBUNIT.

    Entry informationi

    Entry nameiMCRG_METBF
    AccessioniPrimary (citable) accession number: P07964
    Secondary accession number(s): Q46E24
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3