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P07964

- MCRG_METBF

UniProt

P07964 - MCRG_METBF

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Protein

Methyl-coenzyme M reductase subunit gamma

Gene

mcrG

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.

Catalytic activityi

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Pathwayi

GO - Molecular functioni

  1. coenzyme-B sulfoethylthiotransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. methanogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Methanogenesis

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-908-MONOMER.
UniPathwayiUPA00646; UER00699.

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-coenzyme M reductase subunit gamma (EC:2.8.4.1)
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase gamma
Gene namesi
Name:mcrG
Ordered Locus Names:Mbar_A0894
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000008156: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 248247Methyl-coenzyme M reductase subunit gammaPRO_0000147473Add
BLAST

Proteomic databases

PRIDEiP07964.

Interactioni

Subunit structurei

Hexamer of two alpha, two beta, and two gamma chains.1 Publication

Protein-protein interaction databases

STRINGi269797.Mbar_A0894.

Structurei

Secondary structure

1
248
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2311
Helixi34 – 418
Beta strandi52 – 543
Helixi57 – 604
Helixi66 – 705
Helixi75 – 795
Beta strandi83 – 908
Turni92 – 943
Helixi99 – 10911
Beta strandi110 – 1123
Beta strandi114 – 1174
Beta strandi122 – 1276
Helixi128 – 14013
Turni146 – 1483
Beta strandi149 – 1513
Beta strandi176 – 1794
Beta strandi182 – 1876
Beta strandi193 – 1997
Helixi206 – 2127
Helixi223 – 2253
Helixi227 – 24519

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E6YX-ray1.60C/F2-248[»]
ProteinModelPortaliP07964.
SMRiP07964. Positions 2-248.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07964.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG4057.
HOGENOMiHOG000225820.
KOiK00402.
OMAiKRTTIYR.

Family and domain databases

Gene3Di3.90.320.20. 1 hit.
InterProiIPR009024. Me_CoM_Rdtase_Fd-like_fold.
IPR003178. Me_CoM_Rdtase_gsu.
[Graphical view]
PfamiPF02240. MCR_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDomiPD005845. Me_CoM_Rdtase_gsu. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55088. SSF55088. 1 hit.
TIGRFAMsiTIGR03259. met_CoM_red_gam. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07964-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYERQYYPG ATSVAANRRK HMSGKLEKLR EISDEDLTAV LGHRAPGSDY
60 70 80 90 100
PSTHPPLAEM GEPACSTREN VAATPGAAAG DRVRYIQFAD SMYNAPATPY
110 120 130 140 150
FRSYFAAINF RGVDPGTLSG RQIVEARERD MEQCAKVQME TEITDHALAG
160 170 180 190 200
VRGATVHGHS VRLQEDGVMF DMLDRRRLEN GTIIMDKDQV AIPLDRKVDL
210 220 230 240
GKPMSSEEAA KRTTIYRVDN VAFRDDAEVV EWVHRIFDQR TKFGFQPK
Length:248
Mass (Da):27,811
Last modified:January 23, 2007 - v3
Checksum:iAB23870B4BD7160B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00158 Genomic DNA. Translation: CAA68356.1.
CP000099 Genomic DNA. Translation: AAZ69868.1.
PIRiD29525.
RefSeqiWP_011305917.1. NC_007355.1.
YP_304448.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ69868; AAZ69868; Mbar_A0894.
GeneIDi3625939.
KEGGimba:Mbar_A0894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00158 Genomic DNA. Translation: CAA68356.1 .
CP000099 Genomic DNA. Translation: AAZ69868.1 .
PIRi D29525.
RefSeqi WP_011305917.1. NC_007355.1.
YP_304448.1. NC_007355.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E6Y X-ray 1.60 C/F 2-248 [» ]
ProteinModelPortali P07964.
SMRi P07964. Positions 2-248.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269797.Mbar_A0894.

Proteomic databases

PRIDEi P07964.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ69868 ; AAZ69868 ; Mbar_A0894 .
GeneIDi 3625939.
KEGGi mba:Mbar_A0894.

Phylogenomic databases

eggNOGi COG4057.
HOGENOMi HOG000225820.
KOi K00402.
OMAi KRTTIYR.

Enzyme and pathway databases

UniPathwayi UPA00646 ; UER00699 .
BioCyci MBAR269797:GHUW-908-MONOMER.

Miscellaneous databases

EvolutionaryTracei P07964.

Family and domain databases

Gene3Di 3.90.320.20. 1 hit.
InterProi IPR009024. Me_CoM_Rdtase_Fd-like_fold.
IPR003178. Me_CoM_Rdtase_gsu.
[Graphical view ]
Pfami PF02240. MCR_gamma. 1 hit.
[Graphical view ]
PIRSFi PIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDomi PD005845. Me_CoM_Rdtase_gsu. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF55088. SSF55088. 1 hit.
TIGRFAMsi TIGR03259. met_CoM_red_gam. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the methyl coenzyme M reductase gene cluster from Methanosarcina barkeri."
    Bokranz M., Klein A.
    Nucleic Acids Res. 15:4350-4351(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.
  3. "Comparison of three methyl-coenzyme M reductases from phylogenetically distant organisms: unusual amino acid modification, conservation and adaptation."
    Grabarse W., Mahlert F., Shima S., Thauer R.K., Ermler U.
    J. Mol. Biol. 303:329-344(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiMCRG_METBF
AccessioniPrimary (citable) accession number: P07964
Secondary accession number(s): Q46E24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3