Reviewed, UniProtKB/Swiss-Prot P07961 (MCRA_METVA)

Names and origin

Protein names

Recommended name:
    Methyl-coenzyme M reductase subunit alpha
    EC=2.8.4.1
Alternative name(s):
    Coenzyme-B sulfoethylthiotransferase alpha

Gene names

Name:

mcrA

Organism

Methanococcus vannielii

Taxonomic identifier

2187 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide.
Catalytic activity	2-(methylthio)ethanesulfonate (methyl-CoM) + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains.

Ontologies

Keywords
Biological process	Methanogenesis
Ligand	Metal-binding Nickel
Molecular function	Transferase
PTM	Methylation
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	Methyl-coenzyme M reductase subunit alpha		PRO_0000147460
Sites
	Metal binding	151	1	Nickel By similarity
Amino acid modifications
	Modified residue	261	1	Pros-methylhistidine By similarity
	Modified residue	274	1	5-methylarginine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07961-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 7B91F2DDA4B98489	FASTA	553	60,838
	10 20 30 40 50 60 MEAEKRLFLK ALKEKFEEDP KEKYTKFYTY GGWEQSVRKR EFVAANEKVL AEKRQGVPLY 70 80 90 100 110 120 NPDIGVPLGQ RKLMPYKLSG TDSYCEGDDL HFMNNAAIQQ LWDDIRRTVV VGMDTAHSVL 130 140 150 160 170 180 EKRLGVEVTP ETINEYMHTI NHALSGGAVV QEHMVEVHPS LAWDSYARIF TGDDELAAEL 190 200 210 220 230 240 DSRFLIDINK LFPAEQAEAL KKAIGKKTYQ VSRVPSLVGR VCDGGTISRW SAMQIGMSFI 250 260 270 280 290 300 TAYKLCAGEA ATADFSYASK HADVIQMGNA LPGRRARGPN EPGGIQFGIL SDVVQTTRVS 310 320 330 340 350 360 DDPVEQSLEV VAAGAALYDQ IWLGAYMSGG VGFTQYATAA YTDDILDDFS YYALDYVEKK 370 380 390 400 410 420 YGRMGTKATM DVVEDIASEV TLYSLEQYDE YPALLEDHFG GSQRAAVAAA ASGIGVCMAT 430 440 450 460 470 480 GNSNAGVNGW YLSQILHKEY HSRLGFYGYD LQDQCGASNS LAIRNDESAP LELRGPNYPN 490 500 510 520 530 540 YAMNVGHQGE YAGIAQAAHS ARGDAFAMSA LIKVAFADPM LVFDFSKPRK EFARGALREF 550 DAAGERDVIL PAK

References

[1]

"Structure and expression of the genes, mcrBDCGA, which encode the subunits of component C of methyl coenzyme M reductase in Methanococcus vannielii." Cram D.S., Sherf B.A., Libby R.T., Mattaliano R.J., Ramachandran K.L., Reeve J.N. Proc. Natl. Acad. Sci. U.S.A. 84:3992-3996(1987) [PubMed: 16593847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16893 Genomic DNA. Translation: AAA72598.1.
PIR	E27793.
3D structure databases
HSSP	HSSP built from PDB template 1HBN based on UniProtKB P11558.
SMR	P07961. Positions 6-552.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.8.4.1. 165025.
Family and domain databases
InterPro	IPR016212. Me_CoM_Rdtase_asu. IPR008924. Me_CoM_Rdtase_asu/bsu_C. IPR009047. Me_CoM_Rdtase_asu_C. IPR003183. Me_CoM_Rdtase_asu_N. IPR015811. Me_CoM_Rdtase_asu_N_sub1. IPR015823. Me_CoM_Rdtase_asu_N_sub2. [Graphical view]
Gene3D	G3DSA:1.20.840.10. MCR_a/b_chain_a-bundle. 1 hit. G3DSA:3.90.390.10. Me_CoM_Rdtase_asu_N_sub1. 1 hit. G3DSA:3.30.70.470. Me_CoM_Rdtase_asu_N_sub2. 1 hit.
Pfam	PF02249. MCR_alpha. 1 hit. PF02745. MCR_alpha_N. 1 hit. [Graphical view]
PIRSF	PIRSF000262. MCR_alpha. 1 hit.
TIGRFAMs	TIGR03256. met_CoM_red_alp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRA_METVA

Accession

Primary (citable) accession number: P07961

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	September 22, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)