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P07956 (MCRB_METVA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-coenzyme M reductase subunit beta

EC=2.8.4.1
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase beta
Gene names
Name:mcrB
OrganismMethanococcus vannielii
Taxonomic identifier2187 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.

Catalytic activity

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Cofactor

Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.

Pathway

One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.

Subunit structure

Hexamer of two alpha, two beta, and two gamma chains.

Ontologies

Keywords
   Biological processMethanogenesis
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processmethanogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncoenzyme-B sulfoethylthiotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443Methyl-coenzyme M reductase subunit beta
PRO_0000147471

Sequences

Sequence LengthMass (Da)Tools
P07956 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 7646A0D427A98517

FASTA44346,954
        10         20         30         40         50         60 
MVKYEDKISL YDAKGNLVAE NVPLEAISPL YNPTIKSMLK NIKRTVAVNL ADIENTLATG 

        70         80         90        100        110        120 
SIGGKGCKVP GRTLDLSVVS NAQAIADEVE KILKVSKDDD TAIKLINGGK QMAVQVPSER 

       130        140        150        160        170        180 
LEVAAEYSVS MLATAMALKE AIIKTFNVDM FEGSTVHASI MGNYPQVMDY AGGNIASLLG 

       190        200        210        220        230        240 
APSNLEGLGY ALRNIPVNHA VATTKKNMMN AIAFSSVMEQ TATFEMGDAI GSFERQHLLG 

       250        260        270        280        290        300 
LAYQGLNADN LVIEFIKANG KGTVGTVVQS VVERALADGV IVVDKTMGSG FNMYKPADVN 

       310        320        330        340        350        360 
KWNAYAAAGL VAAAAVSCGA ARAAQNIASV ILYYNDILEY ETGLPGVDYG RSMGTAVGFS 

       370        380        390        400        410        420 
FFSHSIYGGG GPGIFNGNHV VTRHSKGFAI PPVCAAMCAD AGTQMFSPEH TSALVGAVYS 

       430        440 
AFDEFREPMK YVIERALNIK DKL 

« Hide

References

[1]"Structure and expression of the genes, mcrBDCGA, which encode the subunits of component C of methyl coenzyme M reductase in Methanococcus vannielii."
Cram D.S., Sherf B.A., Libby R.T., Mattaliano R.J., Ramachandran K.L., Reeve J.N.
Proc. Natl. Acad. Sci. U.S.A. 84:3992-3996(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16893 Genomic DNA. Translation: AAA72594.1.
PIRA27793.

3D structure databases

ProteinModelPortalP07956.
SMRP07956. Positions 3-443.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00646; UER00699.

Family and domain databases

Gene3D1.20.840.10. 2 hits.
3.30.70.470. 1 hit.
InterProIPR022681. MCR_a/b_chain_a-bundle.
IPR008924. Me_CoM_Rdtase_asu/bsu_C.
IPR015823. Me_CoM_Rdtase_asu_N_sub2.
IPR003179. Me_CoM_Rdtase_bsu.
IPR022679. Me_CoM_Rdtase_bsu_C.
IPR022680. Me_CoM_Rdtase_bsu_N.
IPR009024. Me_CoM_Rdtase_Fd-like_fold.
[Graphical view]
PfamPF02241. MCR_beta. 1 hit.
PF02783. MCR_beta_N. 1 hit.
[Graphical view]
PIRSFPIRSF000263. Meth_CoM_rd_beta. 1 hit.
SUPFAMSSF48081. SSF48081. 1 hit.
SSF55088. SSF55088. 1 hit.
TIGRFAMsTIGR03257. met_CoM_red_bet. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMCRB_METVA
AccessionPrimary (citable) accession number: P07956
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: October 16, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways