P07956 (MCRB_METVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methyl-coenzyme M reductase subunit beta
EC=2.8.4.1

Alternative name(s):
Coenzyme-B sulfoethylthiotransferase beta

Gene names

Name:

mcrB

Organism

Methanococcus vannielii

Taxonomic identifier

2187 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide.
Catalytic activity	Methyl-CoM + CoB = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Methyl-coenzyme M reductase subunit beta		PRO_0000147471

Sequences

	Sequence		Length	Mass (Da)
	P07956 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 7646A0D427A98517	FASTA	443	46,954
	10 20 30 40 50 60 MVKYEDKISL YDAKGNLVAE NVPLEAISPL YNPTIKSMLK NIKRTVAVNL ADIENTLATG 70 80 90 100 110 120 SIGGKGCKVP GRTLDLSVVS NAQAIADEVE KILKVSKDDD TAIKLINGGK QMAVQVPSER 130 140 150 160 170 180 LEVAAEYSVS MLATAMALKE AIIKTFNVDM FEGSTVHASI MGNYPQVMDY AGGNIASLLG 190 200 210 220 230 240 APSNLEGLGY ALRNIPVNHA VATTKKNMMN AIAFSSVMEQ TATFEMGDAI GSFERQHLLG 250 260 270 280 290 300 LAYQGLNADN LVIEFIKANG KGTVGTVVQS VVERALADGV IVVDKTMGSG FNMYKPADVN 310 320 330 340 350 360 KWNAYAAAGL VAAAAVSCGA ARAAQNIASV ILYYNDILEY ETGLPGVDYG RSMGTAVGFS 370 380 390 400 410 420 FFSHSIYGGG GPGIFNGNHV VTRHSKGFAI PPVCAAMCAD AGTQMFSPEH TSALVGAVYS 430 440 AFDEFREPMK YVIERALNIK DKL

References

[1]	"Structure and expression of the genes, mcrBDCGA, which encode the subunits of component C of methyl coenzyme M reductase in Methanococcus vannielii." Cram D.S., Sherf B.A., Libby R.T., Mattaliano R.J., Ramachandran K.L., Reeve J.N. Proc. Natl. Acad. Sci. U.S.A. 84:3992-3996(1987) [PubMed: 16593847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16893 Genomic DNA. Translation: AAA72594.1.
PIR	A27793.
3D structure databases
ProteinModelPortal	P07956.
SMR	P07956. Positions 3-443.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022681. MCR_a/b_chain_a-bundle. IPR008924. Me_CoM_Rdtase_asu/bsu_C. IPR015823. Me_CoM_Rdtase_asu_N_sub2. IPR003179. Me_CoM_Rdtase_bsu. IPR022679. Me_CoM_Rdtase_bsu_C. IPR022680. Me_CoM_Rdtase_bsu_N. IPR009024. Me_CoM_Rdtase_Fd-like_fold. [Graphical view]
Gene3D	G3DSA:1.20.840.10. MCR_a/b_chain_a-bundle. 2 hits. G3DSA:3.30.70.470. Me_CoM_Rdtase_asu_N_sub2. 1 hit.
Pfam	PF02241. MCR_beta. 1 hit. PF02783. MCR_beta_N. 1 hit. [Graphical view]
PIRSF	PIRSF000263. Meth_CoM_rd_beta. 1 hit.
SUPFAM	SSF48081. MCR_alpha_beta_C. 1 hit. SSF55088. MCR_fer_like. 1 hit.
TIGRFAMs	TIGR03257. Met_CoM_red_bet. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRB_METVA

Accession

Primary (citable) accession number: P07956

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program