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Protein

Methyl-coenzyme M reductase subunit beta

Gene

mcrB

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.

Catalytic activityi

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Pathwayi: methyl-coenzyme M reduction

This protein is involved in step 1 of the subpathway that synthesizes methane from methyl-coenzyme M.
Proteins known to be involved in this subpathway in this organism are:
  1. Methyl-coenzyme M reductase subunit gamma (mcrG), Methyl-coenzyme M reductase subunit beta (mcrB), Methyl-coenzyme M reductase subunit alpha (mcrA)
This subpathway is part of the pathway methyl-coenzyme M reduction, which is itself part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes methane from methyl-coenzyme M, the pathway methyl-coenzyme M reduction and in One-carbon metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Methanogenesis

Enzyme and pathway databases

BRENDAi2.8.4.1. 3250.
UniPathwayiUPA00646; UER00699.

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-coenzyme M reductase subunit beta (EC:2.8.4.1)
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase beta
Gene namesi
Name:mcrB
Ordered Locus Names:Mbar_A0897
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000008156 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001474622 – 434Methyl-coenzyme M reductase subunit betaAdd BLAST433

Proteomic databases

PRIDEiP07955.

Interactioni

Subunit structurei

Hexamer of two alpha, two beta, and two gamma chains.1 Publication

Protein-protein interaction databases

STRINGi269797.Mbar_A0897.

Structurei

Secondary structure

1434
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Beta strandi14 – 20Combined sources7
Helixi21 – 24Combined sources4
Turni26 – 28Combined sources3
Helixi30 – 41Combined sources12
Beta strandi42 – 46Combined sources5
Helixi47 – 56Combined sources10
Helixi76 – 78Combined sources3
Helixi79 – 90Combined sources12
Beta strandi99 – 103Combined sources5
Turni104 – 107Combined sources4
Beta strandi108 – 112Combined sources5
Helixi115 – 119Combined sources5
Beta strandi121 – 124Combined sources4
Helixi126 – 142Combined sources17
Helixi147 – 149Combined sources3
Helixi150 – 158Combined sources9
Turni159 – 163Combined sources5
Beta strandi164 – 166Combined sources3
Beta strandi171 – 173Combined sources3
Helixi179 – 181Combined sources3
Helixi188 – 190Combined sources3
Helixi194 – 200Combined sources7
Turni201 – 203Combined sources3
Helixi205 – 222Combined sources18
Helixi229 – 243Combined sources15
Helixi248 – 256Combined sources9
Turni257 – 259Combined sources3
Helixi262 – 275Combined sources14
Beta strandi278 – 284Combined sources7
Beta strandi290 – 296Combined sources7
Helixi297 – 319Combined sources23
Helixi322 – 324Combined sources3
Helixi325 – 340Combined sources16
Helixi345 – 348Combined sources4
Helixi349 – 359Combined sources11
Beta strandi362 – 366Combined sources5
Helixi370 – 372Combined sources3
Turni378 – 380Combined sources3
Beta strandi382 – 387Combined sources6
Helixi388 – 397Combined sources10
Helixi406 – 417Combined sources12
Helixi421 – 424Combined sources4
Helixi426 – 431Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E6YX-ray1.60B/E2-434[»]
ProteinModelPortaliP07955.
SMRiP07955.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07955.

Family & Domainsi

Phylogenomic databases

eggNOGiarCOG04860. Archaea.
COG4054. LUCA.
HOGENOMiHOG000225842.
KOiK00401.
OMAiGKQMAVQ.

Family and domain databases

Gene3Di1.20.840.10. 2 hits.
3.30.70.470. 1 hit.
InterProiIPR022681. MCR_a/b_chain_a-bundle.
IPR008924. Me_CoM_Rdtase_asu/bsu_C.
IPR015823. Me_CoM_Rdtase_asu_N_sub2.
IPR003179. Me_CoM_Rdtase_bsu.
IPR022679. Me_CoM_Rdtase_bsu_C.
IPR022680. Me_CoM_Rdtase_bsu_N.
IPR009024. Me_CoM_Rdtase_Fd-like_fold.
[Graphical view]
PfamiPF02241. MCR_beta. 1 hit.
PF02783. MCR_beta_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000263. Meth_CoM_rd_beta. 1 hit.
SUPFAMiSSF48081. SSF48081. 1 hit.
SSF55088. SSF55088. 1 hit.
TIGRFAMsiTIGR03257. met_CoM_red_bet. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07955-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTVDIYDD RGKLLESNVD IMSLAPTRNA AIQSIIMDTK RSVAVNLAGI
60 70 80 90 100
QGALASGKMG GKGRQILGRG LNYDIVGNAD AIAENVKKLV QVDEGDDTNV
110 120 130 140 150
IKVKGGKSLL IQSPKSRIIA GADFMSATTV GAAAVTQTIM DMFGTDPYDA
160 170 180 190 200
PIVKSAVWGS YPQTMDLMGG QVQGILSIPQ NNEGLGFSLR NIMANHVAAI
210 220 230 240 250
SNRNAMNASA LSSIYEQSGI FEMGGAVGMF ERHQLLGLAY QGLNANNLLY
260 270 280 290 300
DIVKENGKDG TIGTVIESVV RRAIEAGIIS VDKTAPSGYN FYKANDVPKW
310 320 330 340 350
NACAAVGTLA ATLVNCGAGR AAQNVSSTLL YFNDILEKET GLPGCDYGKV
360 370 380 390 400
EGTAVGFSFF SHSIYGGGGP GVFNGNHVVT RHSRGFAIPC VCAAVALDAG
410 420 430
TQMFSIESTS GLIGDVFGAI PEFREPIKAV AGVL
Length:434
Mass (Da):45,421
Last modified:January 23, 2007 - v3
Checksum:iA85044187FB78CB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00158 Genomic DNA. Translation: CAA68353.1.
CP000099 Genomic DNA. Translation: AAZ69871.1.
PIRiA29525.
RefSeqiWP_011305920.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ69871; AAZ69871; Mbar_A0897.
GeneIDi3625942.
KEGGimba:Mbar_A0897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00158 Genomic DNA. Translation: CAA68353.1.
CP000099 Genomic DNA. Translation: AAZ69871.1.
PIRiA29525.
RefSeqiWP_011305920.1. NC_007355.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E6YX-ray1.60B/E2-434[»]
ProteinModelPortaliP07955.
SMRiP07955.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269797.Mbar_A0897.

Proteomic databases

PRIDEiP07955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ69871; AAZ69871; Mbar_A0897.
GeneIDi3625942.
KEGGimba:Mbar_A0897.

Phylogenomic databases

eggNOGiarCOG04860. Archaea.
COG4054. LUCA.
HOGENOMiHOG000225842.
KOiK00401.
OMAiGKQMAVQ.

Enzyme and pathway databases

UniPathwayiUPA00646; UER00699.
BRENDAi2.8.4.1. 3250.

Miscellaneous databases

EvolutionaryTraceiP07955.

Family and domain databases

Gene3Di1.20.840.10. 2 hits.
3.30.70.470. 1 hit.
InterProiIPR022681. MCR_a/b_chain_a-bundle.
IPR008924. Me_CoM_Rdtase_asu/bsu_C.
IPR015823. Me_CoM_Rdtase_asu_N_sub2.
IPR003179. Me_CoM_Rdtase_bsu.
IPR022679. Me_CoM_Rdtase_bsu_C.
IPR022680. Me_CoM_Rdtase_bsu_N.
IPR009024. Me_CoM_Rdtase_Fd-like_fold.
[Graphical view]
PfamiPF02241. MCR_beta. 1 hit.
PF02783. MCR_beta_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000263. Meth_CoM_rd_beta. 1 hit.
SUPFAMiSSF48081. SSF48081. 1 hit.
SSF55088. SSF55088. 1 hit.
TIGRFAMsiTIGR03257. met_CoM_red_bet. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMCRB_METBF
AccessioniPrimary (citable) accession number: P07955
Secondary accession number(s): Q46E21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.