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P07951 (TPM2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 152. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tropomyosin beta chain
Alternative name(s):
Beta-tropomyosin
Tropomyosin-2
Gene names
Name:TPM2
Synonyms:TMSB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform mayhave a role in agonist-mediated receptor internalization By similarity.

Subunit structure

Heterodimer of an alpha and a beta chain.

Subcellular location

Cytoplasmcytoskeleton.

Tissue specificity

Present in primary breast cancer tissue, absent from normal breast tissue. Ref.10

Domain

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Post-translational modification

Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization By similarity.

Involvement in disease

Nemaline myopathy 4 (NEM4) [MIM:609285]: A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-like or rod-shaped structures in muscle fibers on histologic examination. Nemaline myopathy type 4 presents from infancy to childhood with hypotonia and moderate-to-severe proximal weakness with minimal or no progression. Major motor milestones are delayed but independent ambulation is usually achieved, although a wheelchair may be needed in later life.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.13

Arthrogryposis, distal, 1A (DA1A) [MIM:108120]: A form of distal arthrogryposis, a disease characterized by congenital joint contractures that mainly involve two or more distal parts of the limbs, in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.14

Sequence similarities

Belongs to the tropomyosin family.

Mass spectrometry

Isoform 1: Molecular mass is 32850.73 Da from positions 1 - 284. Determined by MALDI. Ref.11

Isoform 2: Molecular mass is 32989.81 Da from positions 1 - 284. Determined by MALDI. Ref.11

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P07951-1)

Also known as: Skeletal muscle;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P07951-2)

Also known as: non-muscle; Fibroblast TM36; Epithelial TMe1;

The sequence of this isoform differs from the canonical sequence as follows:
     189-213: KCGDLEEELKIVTNNLKSLEAQADK → RARQLEEELRTMDQALKSLMASEEE
     258-284: DEVYAQKMKYKAISEELDNALNDITSL → ETLASAKEENVEIHQTLDQTLLELNNL
Isoform 3 (identifier: P07951-3)

Also known as: non-muscle;

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MDAIKKKMQM...LEQAEKKATD → MAGISSIDAV...ADAERQARER
     189-213: KCGDLEEELKIVTNNLKSLEAQADK → RARQLEEELRTMDQALKSLMASEEE
     258-284: DEVYAQKMKYKAISEELDNALNDITSL → ETLASAKEENVEIHQTLDQTLLELNNL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 284284Tropomyosin beta chain
PRO_0000205627

Regions

Coiled coil1 – 284284 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue611Phosphoserine; by PIK3CG By similarity

Natural variations

Alternative sequence1 – 8080MDAIK…KKATD → MAGISSIDAVKKKIQSLQQV ADEAEERAEHLQREADAERQ ARER in isoform 3.
VSP_006594
Alternative sequence189 – 21325KCGDL…AQADK → RARQLEEELRTMDQALKSLM ASEEE in isoform 2 and isoform 3.
VSP_006595
Alternative sequence258 – 28427DEVYA…DITSL → ETLASAKEENVEIHQTLDQT LLELNNL in isoform 2 and isoform 3.
VSP_006596
Natural variant911R → G in DA1A. Ref.14
VAR_016086
Natural variant1171E → A in NEM4. Ref.13
VAR_013468
Natural variant1471Q → P in NEM4. Ref.13
VAR_013469
Natural variant2731E → K.
Corresponds to variant rs3180843 [ dbSNP | Ensembl ].
VAR_052402

Experimental info

Sequence conflict891N → S in BAD96978. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Skeletal muscle) [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 18E330568E14E0BE

FASTA28432,851
        10         20         30         40         50         60 
MDAIKKKMQM LKLDKENAID RAEQAEADKK QAEDRCKQLE EEQQALQKKL KGTEDEVEKY 

        70         80         90        100        110        120 
SESVKEAQEK LEQAEKKATD AEADVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA 

       130        140        150        160        170        180 
DESERGMKVI ENRAMKDEEK MELQEMQLKE AKHIAEDSDR KYEEVARKLV ILEGELERSE 

       190        200        210        220        230        240 
ERAEVAESKC GDLEEELKIV TNNLKSLEAQ ADKYSTKEDK YEEEIKLLEE KLKEAETRAE 

       250        260        270        280 
FAERSVAKLE KTIDDLEDEV YAQKMKYKAI SEELDNALND ITSL 

« Hide

Isoform 2 (non-muscle) (Fibroblast TM36) (Epithelial TMe1) [UniParc].

Checksum: 584D60A8A4D6E9CA
Show »

FASTA28432,990
Isoform 3 (non-muscle) [UniParc].

Checksum: D89A89A37CF9D026
Show »

FASTA24828,684

References

« Hide 'large scale' references
[1]"A muscle-type tropomyosin in human fibroblasts: evidence for expression by an alternative RNA splicing mechanism."
MacLeod A.R., Houlker C., Reinach F.C., Smillie L.B., Talbot K., Modi G., Walsh F.S.
Proc. Natl. Acad. Sci. U.S.A. 82:7835-7839(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Fibroblast.
[2]"Complete nucleotide sequence of the adult skeletal isoform of human skeletal muscle beta-tropomyosin."
Widada J.S., Ferraz C., Capony J.-P., Liautard J.-P.
Nucleic Acids Res. 16:3109-3109(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"A cDNA encoding a muscle-type tropomyosin cloned from a human epithelial cell line: identity with human fibroblast tropomyosin TM1."
Prasad G.L., Meissner S., Sheer D.G., Cooper H.L.
Biochem. Biophys. Res. Commun. 177:1068-1075(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Colon epithelium.
[4]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Gastric mucosa.
[5]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Pancreas.
[8]"Nucleotide sequence of the human TPM2 gene."
Ben-Yosef T., Francomano C.A.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-257 (ISOFORM 2).
[9]"A nonmuscle tropomyosin is encoded by the smooth/skeletal beta-tropomyosin gene and its RNA is transcribed from an internal promoter."
Libri D., Mouly V., Lemonnier M., Fiszman M.Y.
J. Biol. Chem. 265:3471-3473(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44 (ISOFORM 3).
[10]"Protein content study revealed the presence of isoform 2 of beta-tropomyosin in primary breast cancer tissues from Sudanese patients."
Ahamed M.E., Mohamed A.O., Ahmed M.E., Sirinuch B., Surasak J.
Sudan J. Med. Sci. 2:183-187(2007)
Cited for: PROTEIN SEQUENCE OF 36-48; 52-65; 141-149 AND 206-217 (ISOFORM 2), TISSUE SPECIFICITY.
Tissue: Mammary cancer.
[11]"Cluster analysis of an extensive human breast cancer cell line protein expression map database."
Harris R.A., Yang A., Stein R.C., Lucy K., Brusten L., Herath A., Parekh R., Waterfield M.D., O'Hare M.J., Neville M.A., Page M.J., Zvelebil M.J.
Proteomics 2:212-223(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
Tissue: Mammary cancer.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Mutations in the beta-tropomyosin (TPM2) gene -- a rare cause of nemaline myopathy."
Donner K., Ollikainen M., Ridanpaeae M., Christen H.J., Goebel H.H., de Visser M., Pelin K., Wallgren-Pettersson C.
Neuromuscul. Disord. 12:151-158(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS NEM4 ALA-117 AND PRO-147.
[14]"Mutations in genes encoding fast-twitch contractile proteins cause distal arthrogryposis syndromes."
Sung S.S., Brassington A.-M.E., Grannatt K., Rutherford A., Whitby F.G., Krakowiak P.A., Jorde L.B., Carey J.C., Bamshad M.
Am. J. Hum. Genet. 72:681-690(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT DA1A GLY-91.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M12126 mRNA. Translation: AAA61229.1.
M12125 mRNA. Translation: AAA36773.1.
X06825 mRNA. Translation: CAA29971.1.
M75165 mRNA. Translation: AAB59509.1.
M74817 mRNA. Translation: AAA61230.1.
AK223258 mRNA. Translation: BAD96978.1.
AL133410 Genomic DNA. Translation: CAI10974.1.
AL133410 Genomic DNA. Translation: CAI10977.1.
CH471071 Genomic DNA. Translation: EAW58354.1.
BC011776 mRNA. Translation: AAH11776.1.
AF209746 Genomic DNA. Translation: AAF17621.1.
J05247 Genomic DNA. Translation: AAA51842.1.
PIRA23562.
S00922.
RefSeqNP_003280.2. NM_003289.3.
NP_998839.1. NM_213674.1.
XP_005251625.1. XM_005251568.1.
UniGeneHs.300772.

3D structure databases

ProteinModelPortalP07951.
SMRP07951. Positions 1-284.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113022. 28 interactions.
IntActP07951. 10 interactions.
MINTMINT-94363.
STRING9606.ENSP00000354219.

PTM databases

PhosphoSiteP07951.

Polymorphism databases

DMDM136090.

2D gel databases

DOSAC-COBS-2DPAGEP07951.
REPRODUCTION-2DPAGEIPI00220709.
UCD-2DPAGEP07951.

Proteomic databases

PaxDbP07951.
PRIDEP07951.

Protocols and materials databases

DNASU7169.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000360958; ENSP00000354219; ENSG00000198467. [P07951-1]
ENST00000378292; ENSP00000367542; ENSG00000198467. [P07951-2]
GeneID7169.
KEGGhsa:7169.
UCSCuc003zxq.3. human. [P07951-2]
uc003zxs.3. human. [P07951-1]

Organism-specific databases

CTD7169.
GeneCardsGC09M035672.
HGNCHGNC:12011. TPM2.
MIM108120. phenotype.
190990. gene.
609285. phenotype.
neXtProtNX_P07951.
Orphanet171881. Cap myopathy.
171439. Childhood-onset nemaline myopathy.
2020. Congenital fiber-type disproportion myopathy.
1146. Digitotalar dysmorphism.
1147. Sheldon-Hall syndrome.
3377. Trismus - pseudocamptodactyly.
171436. Typical nemaline myopathy.
PharmGKBPA36691.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG304012.
HOVERGENHBG107404.
KOK10374.
OMATEPTHEC.
PhylomeDBP07951.
TreeFamTF351519.

Enzyme and pathway databases

ReactomeREACT_17044. Muscle contraction.

Gene expression databases

ArrayExpressP07951.
BgeeP07951.
CleanExHS_TPM2.
GenevestigatorP07951.

Family and domain databases

InterProIPR000533. Tropomyosin.
[Graphical view]
PfamPF00261. Tropomyosin. 1 hit.
[Graphical view]
PRINTSPR00194. TROPOMYOSIN.
PROSITEPS00326. TROPOMYOSIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTPM2. human.
GeneWikiTPM2.
GenomeRNAi7169.
NextBio28086.
PROP07951.
SOURCESearch...

Entry information

Entry nameTPM2_HUMAN
AccessionPrimary (citable) accession number: P07951
Secondary accession number(s): A6NM85 expand/collapse secondary AC list , P06468, Q13894, Q53FM4, Q5TCU4, Q5TCU7, Q9UH67
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: April 16, 2014
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM