P07944 (PBP_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactam-inducible penicillin-binding protein

Gene names

Name:

pbp

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	670 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Induction	In the presence of beta-lactam antibiotics, MRSA cells produce this unique PBP in excessively large amounts and can still proliferate, while all the normal PBPs are inactivated (reversible switching ability of PBP formation).
Miscellaneous	PBP has extremely low affinity to penicillin and most other beta-lactam antibiotics.
Sequence similarities	Belongs to the transpeptidase family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	penicillin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 670	670	Beta-lactam-inducible penicillin-binding protein		PRO_0000195443
Regions
	Transmembrane	4 – 24	21	Helical; Potential
Sites
	Binding site	25	1	Penicillin Probable
	Binding site	405	1	Penicillin Probable

Sequences

	Sequence		Length	Mass (Da)
	P07944 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 2DAA414D35DA993A	FASTA	670	76,463
	10 20 30 40 50 60 MKKIKIVPLI LIVVVVGFGI YFYASKDKEI NNTIDAIEDK NFKQVYKDSS YISKSDNGEV 70 80 90 100 110 120 EMTERPIKIY NSLGVKDINI QDRKIKKVSK NKKRVDAQYK IKTNYGNIDR NVQFNFVKED 130 140 150 160 170 180 GMWKLDWDHS VIIPGMQKDQ SIHIENLKSE RGKILDRNNV ELANTGTHMR LGIVPKNVSK 190 200 210 220 230 240 KDYKAIAKEL SISEDYINNK WIKIGYKMIP SFHFKTVKKM DEYLSDFAKK FHLTTNETES 250 260 270 280 290 300 RNYPLGKATS HLLGYVGPIN SEELKQKEYK GYKDDAVIGK KGLEKLYDKK LQHEDGYRVT 310 320 330 340 350 360 IVRVDDNSNT IAHTLIEKKK KDGKDIQLTI DAKVQKSIYN NMKNDYGSGT AIHPQTGELL 370 380 390 400 410 420 ALVSTPSYDV YPFMYGMSNE EYNKLTEDKK EPLLNKFQIT TSPGSTQKIL TAMIGLNNKT 430 440 450 460 470 480 LDDKTSYKID GKGWQKDKSW GGYNVTRYEV VNGNIDLKQA IESSDNIFFA RVALELGSKK 490 500 510 520 530 540 FEKGMKKLGV GEDIPSDYPF YNAQISNKNL DNEILLADSG YGQGEILINP VQILSIYSAL 550 560 570 580 590 600 ENNGNINAPH LLKDTKNKVW KKNIISKENI NLLNDGMQQV VNKTHKEDIY RSYANLIGKS 610 620 630 640 650 660 GTAELKMKQG ETGRQIGWFI SYDKDNPNMM MAINVKDVQD KGMASYNAKI SGKVYDELYE 670 NGNKKYDIDE

References

[1]	"Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion." Song M.D., Wachi M., Doi M., Ishino F., Matsuhashi M. FEBS Lett. 221:167-171(1987) [PubMed: 3305073] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00688 Genomic DNA. Translation: CAA68684.1.
3D structure databases
ProteinModelPortal	P07944.
SMR	P07944. Positions 27-670.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012338. Beta-lactam/transpept-like. IPR007887. MecA_N. IPR005311. PBP_dimer. IPR001460. PCN-bd_Tpept. [Graphical view]
Gene3D	G3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.
Pfam	PF05223. MecA_N. 1 hit. PF03717. PBP_dimer. 1 hit. PF00905. Transpeptidase. 1 hit. [Graphical view]
SUPFAM	SSF56519. PBP_dimer. 1 hit. SSF56601. PBP_transp_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PBP_STAAU

Accession

Primary (citable) accession number: P07944

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	April 5, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program