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Protein

Aldose reductase

Gene

Akr1b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.

Catalytic activityi

Alditol + NAD(P)+ = aldose + NAD(P)H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491Proton donorBy similarity
Sitei78 – 781Lowers pKa of active site TyrBy similarity
Binding sitei111 – 1111SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 1910NADPSequence Analysis
Nucleotide bindingi211 – 27363NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. alditol:NADP+ 1-oxidoreductase activity Source: RGD

GO - Biological processi

  1. cellular response to hydrogen peroxide Source: RGD
  2. cellular response to methylglyoxal Source: RGD
  3. cellular response to peptide Source: RGD
  4. inner medullary collecting duct development Source: RGD
  5. maternal process involved in female pregnancy Source: RGD
  6. monosaccharide metabolic process Source: RGD
  7. naphthalene metabolic process Source: RGD
  8. norepinephrine metabolic process Source: RGD
  9. positive regulation of JAK-STAT cascade Source: RGD
  10. positive regulation of smooth muscle cell proliferation Source: RGD
  11. response to organic substance Source: RGD
  12. response to thyroid hormone Source: RGD
  13. response to water deprivation Source: RGD
  14. sorbitol biosynthetic process Source: RGD
  15. stress-activated protein kinase signaling cascade Source: RGD
  16. tissue homeostasis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_248129. Pregnenolone biosynthesis.
SABIO-RKP07943.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldose reductase (EC:1.1.1.21)
Short name:
AR
Alternative name(s):
Aldehyde reductase
Gene namesi
Name:Akr1b1
Synonyms:Akr1b4, Aldr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi2092. Akr1b1.

Subcellular locationi

GO - Cellular componenti

  1. cell projection cytoplasm Source: RGD
  2. cytosol Source: RGD
  3. extracellular space Source: RGD
  4. mast cell granule Source: RGD
  5. paranodal junction Source: RGD
  6. perinuclear region of cytoplasm Source: RGD
  7. Schmidt-Lanterman incisure Source: RGD
  8. Schwann cell microvillus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 316315Aldose reductasePRO_0000124627Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei95 – 951N6-acetyllysineBy similarity
Modified residuei222 – 2221N6-acetyllysineBy similarity
Modified residuei263 – 2631N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP07943.
PRIDEiP07943.

PTM databases

PhosphoSiteiP07943.

Expressioni

Gene expression databases

GenevestigatoriP07943.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi246382. 2 interactions.
STRINGi10116.ENSRNOP00000012879.

Structurei

3D structure databases

ProteinModelPortaliP07943.
SMRiP07943. Positions 1-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiCOG0656.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiP07943.
KOiK00011.
OMAiEMREDED.
OrthoDBiEOG70KGQF.
PhylomeDBiP07943.
TreeFamiTF106492.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASHLELNNG TKMPTLGLGT WKSPPGQVTE AVKVAIDMGY RHIDCAQVYQ
60 70 80 90 100
NEKEVGVALQ EKLKEQVVKR QDLFIVSKLW CTFHDQSMVK GACQKTLSDL
110 120 130 140 150
QLDYLDLYLI HWPTGFKPGP DYFPLDASGN VIPSDTDFVD TWTAMEQLVD
160 170 180 190 200
EGLVKAIGVS NFNPLQIERI LNKPGLKYKP AVNQIECHPY LTQEKLIEYC
210 220 230 240 250
HCKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKEIAAK YNKTTAQVLI
260 270 280 290 300
RFPIQRNLVV IPKSVTPARI AENFKVFDFE LSNEDMATLL SYNRNWRVCA
310
LMSCAKHKDY PFHAEV
Length:316
Mass (Da):35,797
Last modified:January 23, 2007 - v3
Checksum:iD6D07843B6850002
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05884 mRNA. Translation: CAA29308.1.
M60322 Genomic DNA. Translation: AAA40721.1.
BC062034 mRNA. Translation: AAH62034.1.
PIRiA60603.
RefSeqiNP_036630.1. NM_012498.1.
UniGeneiRn.107801.

Genome annotation databases

EnsembliENSRNOT00000012879; ENSRNOP00000012879; ENSRNOG00000009513.
GeneIDi24192.
KEGGirno:24192.
UCSCiRGD:2092. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05884 mRNA. Translation: CAA29308.1.
M60322 Genomic DNA. Translation: AAA40721.1.
BC062034 mRNA. Translation: AAH62034.1.
PIRiA60603.
RefSeqiNP_036630.1. NM_012498.1.
UniGeneiRn.107801.

3D structure databases

ProteinModelPortaliP07943.
SMRiP07943. Positions 1-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246382. 2 interactions.
STRINGi10116.ENSRNOP00000012879.

Chemistry

BindingDBiP07943.
ChEMBLiCHEMBL2622.

PTM databases

PhosphoSiteiP07943.

Proteomic databases

PaxDbiP07943.
PRIDEiP07943.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012879; ENSRNOP00000012879; ENSRNOG00000009513.
GeneIDi24192.
KEGGirno:24192.
UCSCiRGD:2092. rat.

Organism-specific databases

CTDi231.
RGDi2092. Akr1b1.

Phylogenomic databases

eggNOGiCOG0656.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiP07943.
KOiK00011.
OMAiEMREDED.
OrthoDBiEOG70KGQF.
PhylomeDBiP07943.
TreeFamiTF106492.

Enzyme and pathway databases

ReactomeiREACT_248129. Pregnenolone biosynthesis.
SABIO-RKP07943.

Miscellaneous databases

NextBioi602571.
PROiP07943.

Gene expression databases

GenevestigatoriP07943.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Aldose reductase and p-crystallin belong to the same protein superfamily as aldehyde reductase."
    Carper D., Nishimura C., Shinohara T., Dietzchold B., Wistow G., Craft C., Kador P., Kinoshita J.H.
    FEBS Lett. 220:209-213(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Lens.
  2. "Characterization of the aldose reductase-encoding gene family in rat."
    Graham C.E., Szpirer C., Levan G., Carper D.
    Gene 107:259-267(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  4. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 42-53; 156-169; 178-195; 276-294 AND 307-316, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.
  5. "Long-term induction of an aldose reductase protein by basic fibroblast growth factor in rat astrocytes in vitro."
    Laeng P., Bouillon P., Taupenot L., Labourdette G.
    Electrophoresis 16:1240-1250(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 156-169 AND 205-210.
    Tissue: Astrocyte.

Entry informationi

Entry nameiALDR_RAT
AccessioniPrimary (citable) accession number: P07943
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.