P07943 (ALDR_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aldose reductase
Short name=AR
EC=1.1.1.21

Alternative name(s):
Aldehyde reductase

Gene names

Name:	Akr1b1
Synonyms:	Akr1b4, Aldr1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Catalytic activity	Alditol + NAD(P)+ = aldose + NAD(P)H.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of JAK-STAT cascade Inferred from mutant phenotype PubMed 15746188. Source: RGD positive regulation of smooth muscle cell proliferation Inferred from mutant phenotype PubMed 16648138. Source: RGD response to organic substance Inferred from direct assay PubMed 16567803. Source: RGD sorbitol biosynthetic process Inferred from mutant phenotype PubMed 6690344. Source: RGD stress-activated protein kinase signaling cascade Inferred from mutant phenotype PubMed 16669970. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from direct assay PubMed 16332428. Source: RGD
Molecular_function	alditol:NADP+ 1-oxidoreductase activity Inferred from direct assay PubMed 2114645. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 316	315	Aldose reductase		PRO_0000124627
Regions
	Nucleotide binding	10 – 19	10	NADP Potential
	Nucleotide binding	211 – 273	63	NADP By similarity
Sites
	Active site	49	1	Proton donor By similarity
	Binding site	111	1	Substrate By similarity
	Site	78	1	Lowers pKa of active site Tyr By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	95	1	N6-acetyllysine By similarity
	Modified residue	222	1	N6-acetyllysine By similarity
	Modified residue	263	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07943 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D6D07843B6850002	FASTA	316	35,797
	10 20 30 40 50 60 MASHLELNNG TKMPTLGLGT WKSPPGQVTE AVKVAIDMGY RHIDCAQVYQ NEKEVGVALQ 70 80 90 100 110 120 EKLKEQVVKR QDLFIVSKLW CTFHDQSMVK GACQKTLSDL QLDYLDLYLI HWPTGFKPGP 130 140 150 160 170 180 DYFPLDASGN VIPSDTDFVD TWTAMEQLVD EGLVKAIGVS NFNPLQIERI LNKPGLKYKP 190 200 210 220 230 240 AVNQIECHPY LTQEKLIEYC HCKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKEIAAK 250 260 270 280 290 300 YNKTTAQVLI RFPIQRNLVV IPKSVTPARI AENFKVFDFE LSNEDMATLL SYNRNWRVCA 310 LMSCAKHKDY PFHAEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Aldose reductase and p-crystallin belong to the same protein superfamily as aldehyde reductase." Carper D., Nishimura C., Shinohara T., Dietzchold B., Wistow G., Craft C., Kador P., Kinoshita J.H. FEBS Lett. 220:209-213(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Lens.
[2]	"Characterization of the aldose reductase-encoding gene family in rat." Graham C.E., Szpirer C., Levan G., Carper D. Gene 107:259-267(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[4]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 42-53; 156-169; 178-195; 276-294 AND 307-316, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.
[5]	"Long-term induction of an aldose reductase protein by basic fibroblast growth factor in rat astrocytes in vitro." Laeng P., Bouillon P., Taupenot L., Labourdette G. Electrophoresis 16:1240-1250(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 156-169 AND 205-210. Tissue: Astrocyte.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X05884 mRNA. Translation: CAA29308.1. M60322 Genomic DNA. Translation: AAA40721.1. BC062034 mRNA. Translation: AAH62034.1.
IPI	IPI00231737.
PIR	A60603.
RefSeq	NP_036630.1. NM_012498.1.
UniGene	Rn.107801.
3D structure databases
ProteinModelPortal	P07943.
SMR	P07943. Positions 1-315.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000012879.
PTM databases
PhosphoSite	P07943.
Proteomic databases
PaxDb	P07943.
PRIDE	P07943.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000012879; ENSRNOP00000012879; ENSRNOG00000009513.
GeneID	24192.
KEGG	rno:24192.
UCSC	RGD:2092. rat.
Organism-specific databases
CTD	231.
RGD	2092. Akr1b1.
Phylogenomic databases
eggNOG	COG0656.
GeneTree	ENSGT00670000097881.
HOGENOM	HOG000250272.
HOVERGEN	HBG000020.
InParanoid	P07943.
KO	K00011.
OMA	NQILLAP.
OrthoDB	EOG4VMFFR.
Enzyme and pathway databases
SABIO-RK	P07943.
Gene expression databases
Genevestigator	P07943.
GermOnline	ENSRNOG00000009513. Rattus norvegicus.
Family and domain databases
Gene3D	3.20.20.100. 1 hit.
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. IPR020471. Aldo/keto_reductase_subgr. IPR023210. NADP_OxRdtase_dom. [Graphical view]
PANTHER	PTHR11732. PTHR11732. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PIRSF	PIRSF000097. AKR. 1 hit.
PRINTS	PR00069. ALDKETRDTASE.
SUPFAM	SSF51430. Aldo/ket_red. 1 hit.
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P07943.
ChEMBL	CHEMBL2622.
NextBio	602571.

Entry information

Entry name

ALDR_RAT

Accession

Primary (citable) accession number: P07943

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program