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Protein

Protein sigma-NS

Gene

S3

Organism
Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Protein that binds to ssRNA and may be involved in genome packaging.1 Publication

GO - Molecular functioni

  1. RNA-directed RNA polymerase activity Source: InterPro
  2. single-stranded RNA binding Source: InterPro
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein sigma-NS
Short name:
SigmaNS
Gene namesi
Name:S3
OrganismiReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Taxonomic identifieri10884 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000007253: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Protein sigma-NSPRO_0000222761Add
BLAST

Interactioni

Subunit structurei

Homomultimer (Probable). Interacts with protein mu-NS; in viral inclusions.Curated2 Publications

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1111Important for ssRNA-binding and formation of complexesAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR002507. Reovirus_polyG_pol.
[Graphical view]
PfamiPF01518. PolyG_pol. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07940-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSLRAAIS KIKRDDVGQQ VCPNYVMLRS SVTTKVVRNV VEYQIRTGGF
60 70 80 90 100
FSCLAMLRPL QYAKRERLLG QRNLERISTR DILQTRDLHS LCMPTPDAPM
110 120 130 140 150
SNHQAATMRE LICSYFKVDH TDGLKYIPMD ERYSPSSLAR LFTMGMAGLH
160 170 180 190 200
ITTEPSYKRV PIMHLAADLD CMTLALPYMI TLDGDTVVPV APTLSAEQLL
210 220 230 240 250
DDGLKGLACM DISYGCEVDA SNRSAGDQSM DSSRCINELY CEETAEAICV
260 270 280 290 300
LKTCLVLNCM QFKLEMDDLA HNATELDKIQ MMIPFSERVF RMASSFATID
310 320 330 340 350
AQCFRFCVMM KDKNLKIDMR ETMRLWTRSA LDDSVVTSSL SISLDRGRWV
360
AADATDARLL VFPIRV
Length:366
Mass (Da):41,189
Last modified:August 1, 1988 - v1
Checksum:iB9172A6A9251CC9A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti250 – 2501V → I in AAA47281 (PubMed:3686825).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14325 Genomic RNA. Translation: AAA47273.1.
M18389 Genomic RNA. Translation: AAA47281.1.
PIRiA25068. MNXRST.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14325 Genomic RNA. Translation: AAA47273.1.
M18389 Genomic RNA. Translation: AAA47281.1.
PIRiA25068. MNXRST.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR002507. Reovirus_polyG_pol.
[Graphical view]
PfamiPF01518. PolyG_pol. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Biosynthesis of reovirus-specified polypeptides. Molecular cDNA cloning and nucleotide sequence of the reovirus serotype 1 Lang strain s3 mRNA which encodes the nonstructural RNA-binding protein sigma NS."
    George C.X., Atwater J.A., Samuel C.E.
    Biochem. Biophys. Res. Commun. 139:845-851(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Comparison of the reovirus serotype 1, 2, and 3 S3 genome segments encoding the nonstructural protein sigma NS."
    Wiener J.R., Joklik W.K.
    Virology 161:332-339(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Amino terminus of reovirus nonstructural protein sigma NS is important for ssRNA binding and nucleoprotein complex formation."
    Gillian A.L., Nibert M.L.
    Virology 240:1-11(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  4. "Reovirus sigma NS protein localizes to inclusions through an association requiring the mu NS amino terminus."
    Miller C.L., Broering T.J., Parker J.S.L., Arnold M.M., Nibert M.L.
    J. Virol. 77:4566-4576(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PROTEIN MU-NS.

Entry informationi

Entry nameiSIGNS_REOVL
AccessioniPrimary (citable) accession number: P07940
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: January 7, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.