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P07939 (SIGM3_REOVL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid protein sigma-3

Short name=Sigma3
Gene names
Name:S4
OrganismReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1) [Reference proteome]
Taxonomic identifier10884 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stimulates translation by blocking the activation of the dsRNA-dependent protein kinase EIF2AK2/PKR, thereby inhibiting the host interferon response. Sigma3 prevents the activation of EIF2AK2 by competing with the kinase for dsRNA-binding By similarity. Ref.3

The viral outer shell polypeptides, of which sigma-3 is one, impose structural constraints that prevent elongation of nascent transcripts by the RNA-dependent RNA polymerase lambda-3. Ref.3

Subunit structure

Heterohexamer of three sigma-3 and three Mu-1 proteins. The RNA-binding form is probably a homodimer By similarity.

Subcellular location

Virion By similarity. Note: Found in the outer capsid. Each subunit is positioned with the small lobe anchoring it to the protein mu1 on the surface of the virion, and the large lobe, the site of initial cleavages during entry-related proteolytic disassembly, protruding outwards By similarity.

Post-translational modification

Cleaved during virus the endosomal proteolytic disassembly of the outer capsid By similarity.

Sequence similarities

Belongs to the orthoreovirus sigma-3 protein family.

Contains 1 CCHC-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365Outer capsid protein sigma-3
PRO_0000222754

Regions

Zinc finger51 – 7323CCHC-type By similarity

Experimental info

Sequence conflict3251D → N in CAA43783. Ref.2

Secondary structure

..................................................................... 365
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07939 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 859808F2BF92B141

FASTA36541,156
        10         20         30         40         50         60 
MEVCLPNGHQ IVDLINNAFE GRVSIYSAQE GWDKTISAQP DMMVCGGAVV CMHCLGVVGS 

        70         80         90        100        110        120 
LQRKLKHLPH HRCNQQIRHQ DYVDVQFADR VTAHWKRGML SFVAQMHAMM NDVSPEDLDR 

       130        140        150        160        170        180 
VRTEGGSLVE LNWLQVDPNS MFRSIHSSWT DPLQVVDDLD TKLDQYWTAL NLMIDSSDLV 

       190        200        210        220        230        240 
PNFMMRDPSH AFNGVRLEGD ARQTQFSRTF DSRSSLEWGV MVYDYSELEH DPSKGRAYRK 

       250        260        270        280        290        300 
ELVTPARDFG HFGLSHYSRA TTPILGKMPA VFSGMLTGNC KMYPFIKGTA KLKTVRKLVD 

       310        320        330        340        350        360 
SVNHAWGVEK IRYALGPGGM TGWYDRTMQQ APIVLTPAAL TMFSDTTKFG DLDYPVMIGD 


PMILG 

« Hide

References

[1]"Biosynthesis of reovirus-specified polypeptides. Molecular cDNA cloning and nucleotide sequence of the reovirus serotype 1 Lang strain s4 mRNA which encodes the major capsid surface polypeptide sigma 3."
Atwater J.A., Manemitsu S.M., Samuel C.E.
Biochem. Biophys. Res. Commun. 136:183-192(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Translational effects and sequence comparisons of the three serotypes of the reovirus S4 gene."
Seliger L.S., Giantini M., Shatkin A.J.
Virology 187:202-210(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"Transcriptional activities of reovirus RNA polymerase in recoated cores. Initiation and elongation are regulated by separate mechanisms."
Farsetta D.L., Chandran K., Nibert M.L.
J. Biol. Chem. 275:39693-39701(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Structure of the reovirus membrane-penetration protein, Mu1, in a complex with is protector protein, Sigma3."
Liemann S., Chandran K., Baker T.S., Nibert M.L., Harrison S.C.
Cell 108:283-295(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH MU-1.
[5]"Features of reovirus outer capsid protein mu1 revealed by electron cryomicroscopy and image reconstruction of the virion at 7.0 Angstrom resolution."
Zhang X., Ji Y., Zhang L., Harrison S.C., Marinescu D.C., Nibert M.L., Baker T.S.
Structure 13:1545-1557(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.0 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M13139 Genomic RNA. Translation: AAA47272.1.
X61586 Genomic RNA. Translation: CAA43783.1.
PIRMNXRS4. A24245.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JMUX-ray2.80G/H/I1-365[»]
2CSEelectron microscopy7.00D/E/F/G/H/I/M/N/O/S1-365[»]
ProteinModelPortalP07939.
SMRP07939. Positions 1-365.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP07939. 10 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000153. Reo_capsid_sigma3.
IPR023634. Reovirus_capsid_sigma-3_dom.
[Graphical view]
PfamPF00979. Reovirus_cap. 1 hit.
[Graphical view]
SUPFAMSSF64465. SSF64465. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP07939.

Entry information

Entry nameSIGM3_REOVL
AccessionPrimary (citable) accession number: P07939
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: February 19, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references