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P07939

- SIGM3_REOVL

UniProt

P07939 - SIGM3_REOVL

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Protein

Outer capsid protein sigma-3

Gene

S4

Organism
Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Stimulates translation by blocking the activation of the dsRNA-dependent protein kinase EIF2AK2/PKR, thereby inhibiting the host interferon response. Sigma3 prevents the activation of EIF2AK2 by competing with the kinase for dsRNA-binding (By similarity).By similarity
The viral outer shell polypeptides, of which sigma-3 is one, impose structural constraints that prevent elongation of nascent transcripts by the RNA-dependent RNA polymerase lambda-3.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri51 – 7323CCHC-typeBy similarityAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. structural molecule activity Source: InterPro

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. regulation of translation Source: UniProtKB-KW
  3. suppression by virus of host PKR activity Source: UniProtKB-KW
  4. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
  6. viral life cycle Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host PKR by virus, Interferon antiviral system evasion, Transcription, Transcription regulation, Translation regulation, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein sigma-3
Short name:
Sigma3
Gene namesi
Name:S4
OrganismiReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Taxonomic identifieri10884 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000007253: Genome

Subcellular locationi

Virion By similarity
Note: Found in the outer capsid. Each subunit is positioned with the small lobe anchoring it to the protein mu1 on the surface of the virion, and the large lobe, the site of initial cleavages during entry-related proteolytic disassembly, protruding outwards (By similarity).By similarity

GO - Cellular componenti

  1. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Outer capsid protein sigma-3PRO_0000222754Add
BLAST

Post-translational modificationi

Cleaved during virus the endosomal proteolytic disassembly of the outer capsid.By similarity

Interactioni

Subunit structurei

Heterohexamer of three sigma-3 and three Mu-1 proteins. The RNA-binding form is probably a homodimer (By similarity).By similarity

Protein-protein interaction databases

IntActiP07939. 10 interactions.

Structurei

Secondary structure

1
365
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 1811
Turni19 – 213
Beta strandi25 – 273
Turni28 – 303
Beta strandi41 – 455
Beta strandi48 – 514
Turni52 – 543
Beta strandi57 – 637
Helixi79 – 813
Helixi83 – 11028
Helixi115 – 1239
Beta strandi126 – 1305
Helixi133 – 1353
Turni141 – 1433
Helixi159 – 17618
Beta strandi180 – 1867
Helixi189 – 1924
Helixi199 – 2035
Beta strandi221 – 2233
Helixi226 – 2305
Helixi232 – 2343
Helixi236 – 2427
Helixi246 – 2494
Helixi252 – 2543
Beta strandi259 – 2613
Beta strandi270 – 2723
Helixi275 – 2773
Beta strandi282 – 2843
Helixi288 – 2925
Helixi293 – 2953
Helixi296 – 30510
Helixi308 – 3158
Helixi319 – 33416
Helixi338 – 3414
Beta strandi351 – 3544
Beta strandi356 – 3583
Beta strandi362 – 3654

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JMUX-ray2.80G/H/I1-365[»]
2CSEelectron microscopy7.00D/E/F/G/H/I/M/N/O/S1-365[»]
ProteinModelPortaliP07939.
SMRiP07939. Positions 1-365.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07939.

Family & Domainsi

Sequence similaritiesi

Contains 1 CCHC-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri51 – 7323CCHC-typeBy similarityAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR000153. Reo_capsid_sigma3.
IPR023634. Reovirus_capsid_sigma-3_dom.
[Graphical view]
PfamiPF00979. Reovirus_cap. 1 hit.
[Graphical view]
SUPFAMiSSF64465. SSF64465. 1 hit.

Sequencei

Sequence statusi: Complete.

P07939-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEVCLPNGHQ IVDLINNAFE GRVSIYSAQE GWDKTISAQP DMMVCGGAVV
60 70 80 90 100
CMHCLGVVGS LQRKLKHLPH HRCNQQIRHQ DYVDVQFADR VTAHWKRGML
110 120 130 140 150
SFVAQMHAMM NDVSPEDLDR VRTEGGSLVE LNWLQVDPNS MFRSIHSSWT
160 170 180 190 200
DPLQVVDDLD TKLDQYWTAL NLMIDSSDLV PNFMMRDPSH AFNGVRLEGD
210 220 230 240 250
ARQTQFSRTF DSRSSLEWGV MVYDYSELEH DPSKGRAYRK ELVTPARDFG
260 270 280 290 300
HFGLSHYSRA TTPILGKMPA VFSGMLTGNC KMYPFIKGTA KLKTVRKLVD
310 320 330 340 350
SVNHAWGVEK IRYALGPGGM TGWYDRTMQQ APIVLTPAAL TMFSDTTKFG
360
DLDYPVMIGD PMILG
Length:365
Mass (Da):41,156
Last modified:August 1, 1988 - v1
Checksum:i859808F2BF92B141
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti325 – 3251D → N in CAA43783. (PubMed:1736524)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13139 Genomic RNA. Translation: AAA47272.1.
X61586 Genomic RNA. Translation: CAA43783.1.
PIRiA24245. MNXRS4.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13139 Genomic RNA. Translation: AAA47272.1 .
X61586 Genomic RNA. Translation: CAA43783.1 .
PIRi A24245. MNXRS4.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JMU X-ray 2.80 G/H/I 1-365 [» ]
2CSE electron microscopy 7.00 D/E/F/G/H/I/M/N/O/S 1-365 [» ]
ProteinModelPortali P07939.
SMRi P07939. Positions 1-365.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P07939. 10 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P07939.

Family and domain databases

InterProi IPR000153. Reo_capsid_sigma3.
IPR023634. Reovirus_capsid_sigma-3_dom.
[Graphical view ]
Pfami PF00979. Reovirus_cap. 1 hit.
[Graphical view ]
SUPFAMi SSF64465. SSF64465. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Biosynthesis of reovirus-specified polypeptides. Molecular cDNA cloning and nucleotide sequence of the reovirus serotype 1 Lang strain s4 mRNA which encodes the major capsid surface polypeptide sigma 3."
    Atwater J.A., Manemitsu S.M., Samuel C.E.
    Biochem. Biophys. Res. Commun. 136:183-192(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Translational effects and sequence comparisons of the three serotypes of the reovirus S4 gene."
    Seliger L.S., Giantini M., Shatkin A.J.
    Virology 187:202-210(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Transcriptional activities of reovirus RNA polymerase in recoated cores. Initiation and elongation are regulated by separate mechanisms."
    Farsetta D.L., Chandran K., Nibert M.L.
    J. Biol. Chem. 275:39693-39701(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Structure of the reovirus membrane-penetration protein, Mu1, in a complex with is protector protein, Sigma3."
    Liemann S., Chandran K., Baker T.S., Nibert M.L., Harrison S.C.
    Cell 108:283-295(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH MU-1.
  5. "Features of reovirus outer capsid protein mu1 revealed by electron cryomicroscopy and image reconstruction of the virion at 7.0 Angstrom resolution."
    Zhang X., Ji Y., Zhang L., Harrison S.C., Marinescu D.C., Nibert M.L., Baker T.S.
    Structure 13:1545-1557(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.0 ANGSTROMS).

Entry informationi

Entry nameiSIGM3_REOVL
AccessioniPrimary (citable) accession number: P07939
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: October 1, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3