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P07934 (PHKG1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

EC=2.7.11.19
Alternative name(s):
Phosphorylase kinase subunit gamma-1
Serine/threonine-protein kinase PHKG1
EC=2.7.11.1
EC=2.7.11.26
Gene names
Name:Phkg1
Synonyms:Phkg
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 By similarity.

Catalytic activity

2 ATP + phosphorylase b = 2 ADP + phosphorylase a.

ATP + [tau protein] = ADP + [tau protein] phosphate.

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Domain

The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 388387Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
PRO_0000086509

Regions

Domain20 – 288269Protein kinase
Nucleotide binding26 – 349ATP By similarity
Region303 – 32725Calmodulin-binding (domain-N)
Region343 – 36725Calmodulin-binding (domain-C)

Sites

Active site1501Proton acceptor By similarity
Binding site491ATP By similarity

Experimental info

Sequence conflict3131L → V in AAA39926. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P07934 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 96E1256891E43718

FASTA38844,960
        10         20         30         40         50         60 
MTRDDALPDS HSAQTFYENY EPKEILGRGV SSVVRRCIHK PTCQEYAVKI IDITGGGSFS 

        70         80         90        100        110        120 
SEEVQELREA TLKEVDILQK VSGHPNIIQL KDTYETNTFF FLVFDLMKRG ELFDYLTEKV 

       130        140        150        160        170        180 
TLTEKETRKI MRALLEVICT LHKLNIVHRD LKPENILLDD NMNIKLTDFG FSCQLQPGEK 

       190        200        210        220        230        240 
LREVCGTPSY LAPEIIQCSM DDGHPGYGKE VDMWSTGVIM YTLLAGSPPF WHRKQMLMLR 

       250        260        270        280        290        300 
MIMDGKYQFG SPEWDDYSDT VKDLVSRFLV VQPQDRCSAE EALAHPFFQE YVVEEVRHFS 

       310        320        330        340        350        360 
PRGKFKVICL TVLASVKIYY QYRRVKPVTR EIVIRDPYAL RPLRRLIDAY AFRIYGHWVK 

       370        380 
KGQQQNRAAL FENTPKAVLL SLAEEEDF 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of cDNA clones for the catalytic gamma subunit of mouse muscle phosphorylase kinase: expression of mRNA in normal and mutant Phk mice."
Chamberlain J.S., Vantuinen P., Reeves A.A., Philip B.A., Caskey C.T.
Proc. Natl. Acad. Sci. U.S.A. 84:2886-2890(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Skeletal muscle phosphorylase kinase catalytic subunit mRNAs are expressed in heart tissue but not in liver."
Bender P.K., Emerson C.P. Jr.
J. Biol. Chem. 262:8799-8805(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"A B2 repeat insertion generates alternate structures of the mouse muscle gamma-phosphorylase kinase gene."
Maichele A.J., Farwell N.J., Chamberlain J.S.
Genomics 16:139-149(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
Tissue: Muscle.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L08059, L08057, L08058 Genomic DNA. Translation: AAB59721.1.
M16216 mRNA. Translation: AAA39926.1.
J03293 mRNA. Translation: AAA39925.1.
S60494 Genomic DNA. No translation available.
S60492 Genomic DNA. No translation available.
S60493 Genomic DNA. No translation available.
BC055102 mRNA. Translation: AAH55102.1.
PIRA29872.
RefSeqNP_035209.1. NM_011079.2.
UniGeneMm.3159.

3D structure databases

ProteinModelPortalP07934.
SMRP07934. Positions 16-366.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000026617.

PTM databases

PhosphoSiteP07934.

Proteomic databases

PaxDbP07934.
PRIDEP07934.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026617; ENSMUSP00000026617; ENSMUSG00000025537.
GeneID18682.
KEGGmmu:18682.
UCSCuc008ztm.1. mouse.

Organism-specific databases

CTD5260.
MGIMGI:97579. Phkg1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00750000117716.
HOGENOMHOG000233016.
HOVERGENHBG106193.
InParanoidP07934.
KOK00871.
OMAQNRYTAE.
OrthoDBEOG7JMGDM.
PhylomeDBP07934.
TreeFamTF320349.

Enzyme and pathway databases

BRENDA2.7.11.19. 3474.

Gene expression databases

ArrayExpressP07934.
BgeeP07934.
CleanExMM_PHKG1.
GenevestigatorP07934.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24347. PTHR24347. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01049. PHOSPHBKNASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294712.
PROP07934.
SOURCESearch...

Entry information

Entry namePHKG1_MOUSE
AccessionPrimary (citable) accession number: P07934
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot