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Protein

Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

Gene

Phkg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 (By similarity).By similarity

Catalytic activityi

2 ATP + phosphorylase b = 2 ADP + phosphorylase a.
ATP + [tau protein] = ADP + [tau protein] phosphate.
ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49ATPPROSITE-ProRule annotation1
Active sitei150Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 34ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Kinase, Muscle protein, Serine/threonine-protein kinase, Transferase
Biological processCarbohydrate metabolism, Glycogen metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.19. 3474.
ReactomeiR-MMU-70221. Glycogen breakdown (glycogenolysis).

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (EC:2.7.11.19)
Alternative name(s):
Phosphorylase kinase subunit gamma-1
Serine/threonine-protein kinase PHKG1 (EC:2.7.11.1, EC:2.7.11.26)
Gene namesi
Name:Phkg1
Synonyms:Phkg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97579. Phkg1.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000865092 – 388Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoformAdd BLAST387

Proteomic databases

MaxQBiP07934.
PaxDbiP07934.
PeptideAtlasiP07934.
PRIDEiP07934.

PTM databases

PhosphoSitePlusiP07934.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025537.
CleanExiMM_PHKG1.
ExpressionAtlasiP07934. baseline and differential.
GenevisibleiP07934. MM.

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026617.

Structurei

3D structure databases

ProteinModelPortaliP07934.
SMRiP07934.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 288Protein kinasePROSITE-ProRule annotationAdd BLAST269

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni303 – 327Calmodulin-binding (domain-N)Add BLAST25
Regioni343 – 367Calmodulin-binding (domain-C)Add BLAST25

Domaini

The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0599. Eukaryota.
ENOG410Y7VX. LUCA.
GeneTreeiENSGT00840000129744.
HOGENOMiHOG000233016.
HOVERGENiHBG106193.
InParanoidiP07934.
KOiK00871.
OMAiQGFYENY.
OrthoDBiEOG091G07XZ.
PhylomeDBiP07934.
TreeFamiTF320349.

Family and domain databases

InterProiView protein in InterPro
IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
PRINTSiPR01049. PHOSPHBKNASE.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07934-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRDDALPDS HSAQTFYENY EPKEILGRGV SSVVRRCIHK PTCQEYAVKI
60 70 80 90 100
IDITGGGSFS SEEVQELREA TLKEVDILQK VSGHPNIIQL KDTYETNTFF
110 120 130 140 150
FLVFDLMKRG ELFDYLTEKV TLTEKETRKI MRALLEVICT LHKLNIVHRD
160 170 180 190 200
LKPENILLDD NMNIKLTDFG FSCQLQPGEK LREVCGTPSY LAPEIIQCSM
210 220 230 240 250
DDGHPGYGKE VDMWSTGVIM YTLLAGSPPF WHRKQMLMLR MIMDGKYQFG
260 270 280 290 300
SPEWDDYSDT VKDLVSRFLV VQPQDRCSAE EALAHPFFQE YVVEEVRHFS
310 320 330 340 350
PRGKFKVICL TVLASVKIYY QYRRVKPVTR EIVIRDPYAL RPLRRLIDAY
360 370 380
AFRIYGHWVK KGQQQNRAAL FENTPKAVLL SLAEEEDF
Length:388
Mass (Da):44,960
Last modified:January 23, 2007 - v3
Checksum:i96E1256891E43718
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti313L → V in AAA39926 (PubMed:3472241).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08059, L08057, L08058 Genomic DNA. Translation: AAB59721.1.
M16216 mRNA. Translation: AAA39926.1.
J03293 mRNA. Translation: AAA39925.1.
S60494 Genomic DNA. No translation available.
S60492 Genomic DNA. No translation available.
S60493 Genomic DNA. No translation available.
BC055102 mRNA. Translation: AAH55102.1.
CCDSiCCDS19701.1.
PIRiA29872.
RefSeqiNP_035209.1. NM_011079.3.
UniGeneiMm.3159.

Genome annotation databases

EnsembliENSMUST00000026617; ENSMUSP00000026617; ENSMUSG00000025537.
GeneIDi18682.
KEGGimmu:18682.
UCSCiuc008ztm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08059, L08057, L08058 Genomic DNA. Translation: AAB59721.1.
M16216 mRNA. Translation: AAA39926.1.
J03293 mRNA. Translation: AAA39925.1.
S60494 Genomic DNA. No translation available.
S60492 Genomic DNA. No translation available.
S60493 Genomic DNA. No translation available.
BC055102 mRNA. Translation: AAH55102.1.
CCDSiCCDS19701.1.
PIRiA29872.
RefSeqiNP_035209.1. NM_011079.3.
UniGeneiMm.3159.

3D structure databases

ProteinModelPortaliP07934.
SMRiP07934.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026617.

PTM databases

PhosphoSitePlusiP07934.

Proteomic databases

MaxQBiP07934.
PaxDbiP07934.
PeptideAtlasiP07934.
PRIDEiP07934.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026617; ENSMUSP00000026617; ENSMUSG00000025537.
GeneIDi18682.
KEGGimmu:18682.
UCSCiuc008ztm.1. mouse.

Organism-specific databases

CTDi5260.
MGIiMGI:97579. Phkg1.

Phylogenomic databases

eggNOGiKOG0599. Eukaryota.
ENOG410Y7VX. LUCA.
GeneTreeiENSGT00840000129744.
HOGENOMiHOG000233016.
HOVERGENiHBG106193.
InParanoidiP07934.
KOiK00871.
OMAiQGFYENY.
OrthoDBiEOG091G07XZ.
PhylomeDBiP07934.
TreeFamiTF320349.

Enzyme and pathway databases

BRENDAi2.7.11.19. 3474.
ReactomeiR-MMU-70221. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

PROiPR:P07934.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025537.
CleanExiMM_PHKG1.
ExpressionAtlasiP07934. baseline and differential.
GenevisibleiP07934. MM.

Family and domain databases

InterProiView protein in InterPro
IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
PRINTSiPR01049. PHOSPHBKNASE.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPHKG1_MOUSE
AccessioniPrimary (citable) accession number: P07934
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.