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P07934

- PHKG1_MOUSE

UniProt

P07934 - PHKG1_MOUSE

Protein

Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

Gene

Phkg1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 By similarity.By similarity

    Catalytic activityi

    2 ATP + phosphorylase b = 2 ADP + phosphorylase a.
    ATP + [tau protein] = ADP + [tau protein] phosphate.
    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491ATPPROSITE-ProRule annotations
    Active sitei150 – 1501Proton acceptorPROSITE-ProRule annotations

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotations

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphorylase kinase activity Source: UniProtKB-EC
    3. tau-protein kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycogen biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Muscle protein, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen metabolism

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.19. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (EC:2.7.11.19)
    Alternative name(s):
    Phosphorylase kinase subunit gamma-1
    Serine/threonine-protein kinase PHKG1 (EC:2.7.11.1, EC:2.7.11.26)
    Gene namesi
    Name:Phkg1
    Synonyms:Phkg
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

    Organism-specific databases

    MGIiMGI:97579. Phkg1.

    Subcellular locationi

    GO - Cellular componenti

    1. phosphorylase kinase complex Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 388387Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoformPRO_0000086509Add
    BLAST

    Proteomic databases

    MaxQBiP07934.
    PaxDbiP07934.
    PRIDEiP07934.

    PTM databases

    PhosphoSiteiP07934.

    Expressioni

    Gene expression databases

    ArrayExpressiP07934.
    BgeeiP07934.
    CleanExiMM_PHKG1.
    GenevestigatoriP07934.

    Interactioni

    Subunit structurei

    Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000026617.

    Structurei

    3D structure databases

    ProteinModelPortaliP07934.
    SMRiP07934. Positions 16-366.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 288269Protein kinasePROSITE-ProRule annotationsAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni303 – 32725Calmodulin-binding (domain-N)Add
    BLAST
    Regioni343 – 36725Calmodulin-binding (domain-C)Add
    BLAST

    Domaini

    The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains.By similarity

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotations

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117716.
    HOGENOMiHOG000233016.
    HOVERGENiHBG106193.
    InParanoidiP07934.
    KOiK00871.
    OMAiQNRYTAE.
    OrthoDBiEOG7JMGDM.
    PhylomeDBiP07934.
    TreeFamiTF320349.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR002291. Phosph_kin_gamma.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01049. PHOSPHBKNASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07934-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTRDDALPDS HSAQTFYENY EPKEILGRGV SSVVRRCIHK PTCQEYAVKI    50
    IDITGGGSFS SEEVQELREA TLKEVDILQK VSGHPNIIQL KDTYETNTFF 100
    FLVFDLMKRG ELFDYLTEKV TLTEKETRKI MRALLEVICT LHKLNIVHRD 150
    LKPENILLDD NMNIKLTDFG FSCQLQPGEK LREVCGTPSY LAPEIIQCSM 200
    DDGHPGYGKE VDMWSTGVIM YTLLAGSPPF WHRKQMLMLR MIMDGKYQFG 250
    SPEWDDYSDT VKDLVSRFLV VQPQDRCSAE EALAHPFFQE YVVEEVRHFS 300
    PRGKFKVICL TVLASVKIYY QYRRVKPVTR EIVIRDPYAL RPLRRLIDAY 350
    AFRIYGHWVK KGQQQNRAAL FENTPKAVLL SLAEEEDF 388
    Length:388
    Mass (Da):44,960
    Last modified:January 23, 2007 - v3
    Checksum:i96E1256891E43718
    GO

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti313 – 3131L → V in AAA39926. (PubMed:3472241)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08059, L08057, L08058 Genomic DNA. Translation: AAB59721.1.
    M16216 mRNA. Translation: AAA39926.1.
    J03293 mRNA. Translation: AAA39925.1.
    S60494 Genomic DNA. No translation available.
    S60492 Genomic DNA. No translation available.
    S60493 Genomic DNA. No translation available.
    BC055102 mRNA. Translation: AAH55102.1.
    CCDSiCCDS19701.1.
    PIRiA29872.
    RefSeqiNP_035209.1. NM_011079.2.
    UniGeneiMm.3159.

    Genome annotation databases

    EnsembliENSMUST00000026617; ENSMUSP00000026617; ENSMUSG00000025537.
    GeneIDi18682.
    KEGGimmu:18682.
    UCSCiuc008ztm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08059 , L08057 , L08058 Genomic DNA. Translation: AAB59721.1 .
    M16216 mRNA. Translation: AAA39926.1 .
    J03293 mRNA. Translation: AAA39925.1 .
    S60494 Genomic DNA. No translation available.
    S60492 Genomic DNA. No translation available.
    S60493 Genomic DNA. No translation available.
    BC055102 mRNA. Translation: AAH55102.1 .
    CCDSi CCDS19701.1.
    PIRi A29872.
    RefSeqi NP_035209.1. NM_011079.2.
    UniGenei Mm.3159.

    3D structure databases

    ProteinModelPortali P07934.
    SMRi P07934. Positions 16-366.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000026617.

    PTM databases

    PhosphoSitei P07934.

    Proteomic databases

    MaxQBi P07934.
    PaxDbi P07934.
    PRIDEi P07934.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026617 ; ENSMUSP00000026617 ; ENSMUSG00000025537 .
    GeneIDi 18682.
    KEGGi mmu:18682.
    UCSCi uc008ztm.1. mouse.

    Organism-specific databases

    CTDi 5260.
    MGIi MGI:97579. Phkg1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117716.
    HOGENOMi HOG000233016.
    HOVERGENi HBG106193.
    InParanoidi P07934.
    KOi K00871.
    OMAi QNRYTAE.
    OrthoDBi EOG7JMGDM.
    PhylomeDBi P07934.
    TreeFami TF320349.

    Enzyme and pathway databases

    BRENDAi 2.7.11.19. 3474.

    Miscellaneous databases

    NextBioi 294712.
    PROi P07934.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P07934.
    Bgeei P07934.
    CleanExi MM_PHKG1.
    Genevestigatori P07934.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR002291. Phosph_kin_gamma.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01049. PHOSPHBKNASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of cDNA clones for the catalytic gamma subunit of mouse muscle phosphorylase kinase: expression of mRNA in normal and mutant Phk mice."
      Chamberlain J.S., Vantuinen P., Reeves A.A., Philip B.A., Caskey C.T.
      Proc. Natl. Acad. Sci. U.S.A. 84:2886-2890(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Skeletal muscle phosphorylase kinase catalytic subunit mRNAs are expressed in heart tissue but not in liver."
      Bender P.K., Emerson C.P. Jr.
      J. Biol. Chem. 262:8799-8805(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "A B2 repeat insertion generates alternate structures of the mouse muscle gamma-phosphorylase kinase gene."
      Maichele A.J., Farwell N.J., Chamberlain J.S.
      Genomics 16:139-149(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
      Tissue: Muscle.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiPHKG1_MOUSE
    AccessioniPrimary (citable) accession number: P07934
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 139 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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