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P07934

- PHKG1_MOUSE

UniProt

P07934 - PHKG1_MOUSE

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Protein

Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

Gene

Phkg1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 (By similarity).By similarity

Catalytic activityi

2 ATP + phosphorylase b = 2 ADP + phosphorylase a.
ATP + [tau protein] = ADP + [tau protein] phosphate.
ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATPPROSITE-ProRule annotation
Active sitei150 – 1501Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphorylase kinase activity Source: UniProtKB-EC
  3. tau-protein kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycogen biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Muscle protein, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.19. 3474.
ReactomeiREACT_256123. Glycogen breakdown (glycogenolysis).

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (EC:2.7.11.19)
Alternative name(s):
Phosphorylase kinase subunit gamma-1
Serine/threonine-protein kinase PHKG1 (EC:2.7.11.1, EC:2.7.11.26)
Gene namesi
Name:Phkg1
Synonyms:Phkg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:97579. Phkg1.

Subcellular locationi

GO - Cellular componenti

  1. phosphorylase kinase complex Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 388387Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoformPRO_0000086509Add
BLAST

Proteomic databases

MaxQBiP07934.
PaxDbiP07934.
PRIDEiP07934.

PTM databases

PhosphoSiteiP07934.

Expressioni

Gene expression databases

BgeeiP07934.
CleanExiMM_PHKG1.
ExpressionAtlasiP07934. baseline and differential.
GenevestigatoriP07934.

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026617.

Structurei

3D structure databases

ProteinModelPortaliP07934.
SMRiP07934. Positions 16-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 288269Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni303 – 32725Calmodulin-binding (domain-N)Add
BLAST
Regioni343 – 36725Calmodulin-binding (domain-C)Add
BLAST

Domaini

The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120596.
HOGENOMiHOG000233016.
HOVERGENiHBG106193.
InParanoidiP07934.
KOiK00871.
OMAiQNRYTAE.
OrthoDBiEOG7JMGDM.
PhylomeDBiP07934.
TreeFamiTF320349.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01049. PHOSPHBKNASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07934-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRDDALPDS HSAQTFYENY EPKEILGRGV SSVVRRCIHK PTCQEYAVKI
60 70 80 90 100
IDITGGGSFS SEEVQELREA TLKEVDILQK VSGHPNIIQL KDTYETNTFF
110 120 130 140 150
FLVFDLMKRG ELFDYLTEKV TLTEKETRKI MRALLEVICT LHKLNIVHRD
160 170 180 190 200
LKPENILLDD NMNIKLTDFG FSCQLQPGEK LREVCGTPSY LAPEIIQCSM
210 220 230 240 250
DDGHPGYGKE VDMWSTGVIM YTLLAGSPPF WHRKQMLMLR MIMDGKYQFG
260 270 280 290 300
SPEWDDYSDT VKDLVSRFLV VQPQDRCSAE EALAHPFFQE YVVEEVRHFS
310 320 330 340 350
PRGKFKVICL TVLASVKIYY QYRRVKPVTR EIVIRDPYAL RPLRRLIDAY
360 370 380
AFRIYGHWVK KGQQQNRAAL FENTPKAVLL SLAEEEDF
Length:388
Mass (Da):44,960
Last modified:January 23, 2007 - v3
Checksum:i96E1256891E43718
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti313 – 3131L → V in AAA39926. (PubMed:3472241)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08059, L08057, L08058 Genomic DNA. Translation: AAB59721.1.
M16216 mRNA. Translation: AAA39926.1.
J03293 mRNA. Translation: AAA39925.1.
S60494 Genomic DNA. No translation available.
S60492 Genomic DNA. No translation available.
S60493 Genomic DNA. No translation available.
BC055102 mRNA. Translation: AAH55102.1.
CCDSiCCDS19701.1.
PIRiA29872.
RefSeqiNP_035209.1. NM_011079.2.
UniGeneiMm.3159.

Genome annotation databases

EnsembliENSMUST00000026617; ENSMUSP00000026617; ENSMUSG00000025537.
GeneIDi18682.
KEGGimmu:18682.
UCSCiuc008ztm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08059 , L08057 , L08058 Genomic DNA. Translation: AAB59721.1 .
M16216 mRNA. Translation: AAA39926.1 .
J03293 mRNA. Translation: AAA39925.1 .
S60494 Genomic DNA. No translation available.
S60492 Genomic DNA. No translation available.
S60493 Genomic DNA. No translation available.
BC055102 mRNA. Translation: AAH55102.1 .
CCDSi CCDS19701.1.
PIRi A29872.
RefSeqi NP_035209.1. NM_011079.2.
UniGenei Mm.3159.

3D structure databases

ProteinModelPortali P07934.
SMRi P07934. Positions 16-333.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000026617.

PTM databases

PhosphoSitei P07934.

Proteomic databases

MaxQBi P07934.
PaxDbi P07934.
PRIDEi P07934.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026617 ; ENSMUSP00000026617 ; ENSMUSG00000025537 .
GeneIDi 18682.
KEGGi mmu:18682.
UCSCi uc008ztm.1. mouse.

Organism-specific databases

CTDi 5260.
MGIi MGI:97579. Phkg1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00770000120596.
HOGENOMi HOG000233016.
HOVERGENi HBG106193.
InParanoidi P07934.
KOi K00871.
OMAi QNRYTAE.
OrthoDBi EOG7JMGDM.
PhylomeDBi P07934.
TreeFami TF320349.

Enzyme and pathway databases

BRENDAi 2.7.11.19. 3474.
Reactomei REACT_256123. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

NextBioi 294712.
PROi P07934.
SOURCEi Search...

Gene expression databases

Bgeei P07934.
CleanExi MM_PHKG1.
ExpressionAtlasi P07934. baseline and differential.
Genevestigatori P07934.

Family and domain databases

InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01049. PHOSPHBKNASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of cDNA clones for the catalytic gamma subunit of mouse muscle phosphorylase kinase: expression of mRNA in normal and mutant Phk mice."
    Chamberlain J.S., Vantuinen P., Reeves A.A., Philip B.A., Caskey C.T.
    Proc. Natl. Acad. Sci. U.S.A. 84:2886-2890(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Skeletal muscle phosphorylase kinase catalytic subunit mRNAs are expressed in heart tissue but not in liver."
    Bender P.K., Emerson C.P. Jr.
    J. Biol. Chem. 262:8799-8805(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "A B2 repeat insertion generates alternate structures of the mouse muscle gamma-phosphorylase kinase gene."
    Maichele A.J., Farwell N.J., Chamberlain J.S.
    Genomics 16:139-149(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
    Tissue: Muscle.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiPHKG1_MOUSE
AccessioniPrimary (citable) accession number: P07934
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3