P07934 (PHKG1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
EC=2.7.11.19

Alternative name(s):
Phosphorylase kinase subunit gamma-1
Serine/threonine-protein kinase PHKG1
EC=2.7.11.1
EC=2.7.11.26

Gene names

Name:	Phkg1
Synonyms:	Phkg

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 By similarity.
Catalytic activity	2 ATP + phosphorylase b = 2 ADP + phosphorylase a. ATP + [tau protein] = ADP + [tau protein] phosphate. ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.
Domain	The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Muscle protein Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	phosphorylase kinase complex Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorylase kinase activity Inferred from electronic annotation. Source: EC tau-protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 388	387	Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform		PRO_0000086509
Regions
	Domain	20 – 288	269	Protein kinase
	Nucleotide binding	26 – 34	9	ATP By similarity
	Region	303 – 327	25	Calmodulin-binding (domain-N)
	Region	343 – 367	25	Calmodulin-binding (domain-C)
Sites
	Active site	150	1	Proton acceptor By similarity
	Binding site	49	1	ATP By similarity
Experimental info
	Sequence conflict	313	1	L → V in AAA39926. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07934 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 96E1256891E43718	FASTA	388	44,960
	10 20 30 40 50 60 MTRDDALPDS HSAQTFYENY EPKEILGRGV SSVVRRCIHK PTCQEYAVKI IDITGGGSFS 70 80 90 100 110 120 SEEVQELREA TLKEVDILQK VSGHPNIIQL KDTYETNTFF FLVFDLMKRG ELFDYLTEKV 130 140 150 160 170 180 TLTEKETRKI MRALLEVICT LHKLNIVHRD LKPENILLDD NMNIKLTDFG FSCQLQPGEK 190 200 210 220 230 240 LREVCGTPSY LAPEIIQCSM DDGHPGYGKE VDMWSTGVIM YTLLAGSPPF WHRKQMLMLR 250 260 270 280 290 300 MIMDGKYQFG SPEWDDYSDT VKDLVSRFLV VQPQDRCSAE EALAHPFFQE YVVEEVRHFS 310 320 330 340 350 360 PRGKFKVICL TVLASVKIYY QYRRVKPVTR EIVIRDPYAL RPLRRLIDAY AFRIYGHWVK 370 380 KGQQQNRAAL FENTPKAVLL SLAEEEDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of cDNA clones for the catalytic gamma subunit of mouse muscle phosphorylase kinase: expression of mRNA in normal and mutant Phk mice." Chamberlain J.S., Vantuinen P., Reeves A.A., Philip B.A., Caskey C.T. Proc. Natl. Acad. Sci. U.S.A. 84:2886-2890(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Skeletal muscle phosphorylase kinase catalytic subunit mRNAs are expressed in heart tissue but not in liver." Bender P.K., Emerson C.P. Jr. J. Biol. Chem. 262:8799-8805(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"A B2 repeat insertion generates alternate structures of the mouse muscle gamma-phosphorylase kinase gene." Maichele A.J., Farwell N.J., Chamberlain J.S. Genomics 16:139-149(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Muscle.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08059, L08057, L08058 Genomic DNA. Translation: AAB59721.1. M16216 mRNA. Translation: AAA39926.1. J03293 mRNA. Translation: AAA39925.1. S60494 Genomic DNA. No translation available. S60492 Genomic DNA. No translation available. S60493 Genomic DNA. No translation available. BC055102 mRNA. Translation: AAH55102.1.
IPI	IPI00224751.
PIR	A29872.
RefSeq	NP_035209.1. NM_011079.2.
UniGene	Mm.3159.
3D structure databases
ProteinModelPortal	P07934.
SMR	P07934. Positions 16-366.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000026617.
PTM databases
PhosphoSite	P07934.
Proteomic databases
PaxDb	P07934.
PRIDE	P07934.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026617; ENSMUSP00000026617; ENSMUSG00000025537.
GeneID	18682.
KEGG	mmu:18682.
UCSC	uc008ztm.1. mouse.
Organism-specific databases
CTD	5260.
MGI	MGI:97579. Phkg1.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00680000100008.
HOGENOM	HOG000233016.
HOVERGEN	HBG106193.
InParanoid	P07934.
KO	K00871.
OMA	DFYENYE.
OrthoDB	EOG4CNQR5.
Enzyme and pathway databases
BRENDA	2.7.11.19. 3474.
Gene expression databases
ArrayExpress	P07934.
Bgee	P07934.
CleanEx	MM_PHKG1.
Genevestigator	P07934.
GermOnline	ENSMUSG00000025537. Mus musculus.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR002291. Phosph_kin_gamma. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
PANTHER	PTHR24347. PTHR24347. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01049. PHOSPHBKNASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294712.
SOURCE	Search...

Entry information

Entry name

PHKG1_MOUSE

Accession

Primary (citable) accession number: P07934

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 129 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program