Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA polymerase catalytic subunit

Gene

UL30

Organism
Human herpesvirus 2 (strain 186) (HHV-2) (Human herpes simplex virus 2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Replicates viral genomic DNA. The replication complex is composed of six viral proteins: the DNA polymerase, processivity factor, primase, primase-associated factor, helicase, and ssDNA-binding protein. Additionally, the polymerase contains an intrinsic ribonuclease H (RNase H) activity that specifically degrades RNA/DNA heteroduplexes or duplex DNA substrates in the 5' to 3' direction. Therefore, it can catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during viral DNA replication (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Endonucleolytic cleavage to 5'-phosphomonoester.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. nucleotide binding Source: InterPro
  4. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase catalytic subunit (EC:2.7.7.7, EC:3.1.26.4)
Gene namesi
ORF Names:UL30
OrganismiHuman herpesvirus 2 (strain 186) (HHV-2) (Human herpes simplex virus 2)
Taxonomic identifieri10312 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Host nucleus By similarity
Note: The protein is present at discrete sites in nuclei, called replication compartments where viral DNA replication occurs.By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12401240DNA polymerase catalytic subunitPRO_0000046515Add
BLAST

Interactioni

Subunit structurei

Forms a complex with the ssDNA-binding protein UL29, the DNA polymerase processivity factor, and the alkaline exonuclease. Interacts with the putative helicase-primase complex subunit UL8; this interaction may coordinate leading and lagging strand DNA synthesis at the replication fork (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP07918.
SMRiP07918. Positions 61-640, 1205-1240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi991 – 9966Poly-Ala

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR021639. DNAPolymera_Pol_C.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
PF11590. DNAPolymera_Pol. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07918-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFCAAGGPAS PGGKSAARAA SGFFAPHNPR GATQTAPPPC RRQNFYNPHL
60 70 80 90 100
AQTGTQPKAP GPAQRHTYYS ECDEFRFIAP RSLDEDAPAE QRTGVHDGRL
110 120 130 140 150
RRAPKVYCGG DERDVLRVGP EGFWPRRLRL WGGADHAPEG FDPTVTVFHV
160 170 180 190 200
YDILEHVEHA YSMRAAQLHE RFMDAITPAG TVITLLGLTP EGHRVAVHVY
210 220 230 240 250
GTRQYFYMNK AEVDRHLQCR APRDLCERLA AALRESPGAS FRGISADHFE
260 270 280 290 300
AEVVERADVY YYETRPTLYY RVFVRSGRAL AYLCDNFCPA IRKYEGGVDA
310 320 330 340 350
TTRFILDNPG FVTFGWYRLK PGRGNAPAQP RPPTAFGTSS DVEFNCTADN
360 370 380 390 400
LAVEGAMCDL PAYKLMCFDI ECKAGGEDEL AFPVAERPED LVIQISCLLY
410 420 430 440 450
DLSTTALEHI LLFSLGSCDL PESHLSDLAS RGLPAPVVLE FDSEFEMLLA
460 470 480 490 500
FMTFVKQYGP EFVTGYNIIN FDWPFVLTKL TEIYKVPLDG YGRMNGRGVF
510 520 530 540 550
RVWDIGQSHF QKRSKIKVNG MVNIDMYGII TDKVKLSSYK LNAVAEAVLK
560 570 580 590 600
DKKKDLSYRD IPAYYASGPA QRGVIGEYCV QDSLLVGQLF FKFLPHLELS
610 620 630 640 650
AVARLAGINI TRTIYDGQQI RVFTCLLRLA GQKGFILPDT QGRFRGLDKE
660 670 680 690 700
APKRPAVPRG EGERPGDGNG DEDKDDDEDG DEDGDEREEV ARETGGRHVG
710 720 730 740 750
YQGARVLDPT SGFHVDPVVV FDFASLYPSI IQAHNLCFST LSLRPEAVAH
760 770 780 790 800
LEADRDYLEI EVGGRRLFFV KAHVRESLLS ILLRDWLAMR KQIRSRIPQS
810 820 830 840 850
PPEEAVLLDK QQAAIKVVCN SVYGFTGVQH GLLPCLHVAA TVTTIGREML
860 870 880 890 900
LATRAYVHAR WAEFDQLLAD FPEAAGMRAP GPYSMRIIYG DTDSIFVLCR
910 920 930 940 950
GLTGEALVAM GDKMASHISR ALFLPPIKLE CEKTFTKLLL IAKKKYIGVI
960 970 980 990 1000
CGGKMLIKGV DLVRKNNCAF INRTSRALVD LLFYDDTVSG AAAALAERPA
1010 1020 1030 1040 1050
EEWLARPLPE GLQAFGAVLV DAHRRITDPE RDIQDFVLTA ELSRHPRAYT
1060 1070 1080 1090 1100
NKRLAHLTVY YKLMARRAQV PSIKDRIPYV IVAQTREVEE TVARLAALRE
1110 1120 1130 1140 1150
LDAAAPGDEP APPAALPSPA KRPRETPSHA DPPGGASKPR KLLVSELAED
1160 1170 1180 1190 1200
PGYAIARGVP LNTDYYFSHL LGAACVTFKA LFGNNAKITE SLLKRFIPET
1210 1220 1230 1240
WHPPDDVAAR LRAAGFGPAG AGATAEETRR MLHRAFDTLA
Length:1,240
Mass (Da):137,356
Last modified:August 1, 1988 - v1
Checksum:i1CDA4B1D11F8180E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16321 Genomic DNA. Translation: AAA45853.1.
PIRiA27315. DJBE21.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16321 Genomic DNA. Translation: AAA45853.1.
PIRiA27315. DJBE21.

3D structure databases

ProteinModelPortaliP07918.
SMRiP07918. Positions 61-640, 1205-1240.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR021639. DNAPolymera_Pol_C.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
PF11590. DNAPolymera_Pol. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the DNA polymerase gene of herpes simplex virus type 2 and comparison with the type 1 counterpart."
    Tsurumi T., Maeno K., Nishiyama Y.
    Gene 52:129-137(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiDPOL_HHV21
AccessioniPrimary (citable) accession number: P07918
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: January 7, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.