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P07918 (DPOL_HHV21) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA polymerase catalytic subunit

EC=2.7.7.7
EC=3.1.26.4
Gene names
ORF Names:UL30
OrganismHuman herpesvirus 2 (strain 186) (HHV-2) (Human herpes simplex virus 2)
Taxonomic identifier10312 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length1240 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Replicates viral genomic DNA. The replication complex is composed of six viral proteins: the DNA polymerase, processivity factor, primase, primase-associated factor, helicase, and ssDNA-binding protein. Additionally, the polymerase contains an intrinsic ribonuclease H (RNase H) activity that specifically degrades RNA/DNA heteroduplexes or duplex DNA substrates in the 5' to 3' direction. Therefore, it can catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during viral DNA replication By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Endonucleolytic cleavage to 5'-phosphomonoester.

Subunit structure

Forms a complex with the ssDNA-binding protein UL29, the DNA polymerase processivity factor, and the alkaline exonuclease. Interacts with the putative helicase-primase complex subunit UL8; this interaction may coordinate leading and lagging strand DNA synthesis at the replication fork By similarity.

Subcellular location

Host nucleus By similarity. Note: The protein is present at discrete sites in nuclei, called replication compartments where viral DNA replication occurs By similarity.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12401240DNA polymerase catalytic subunit
PRO_0000046515

Regions

Compositional bias991 – 9966Poly-Ala

Sequences

Sequence LengthMass (Da)Tools
P07918 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 1CDA4B1D11F8180E

FASTA1,240137,356
        10         20         30         40         50         60 
MFCAAGGPAS PGGKSAARAA SGFFAPHNPR GATQTAPPPC RRQNFYNPHL AQTGTQPKAP 

        70         80         90        100        110        120 
GPAQRHTYYS ECDEFRFIAP RSLDEDAPAE QRTGVHDGRL RRAPKVYCGG DERDVLRVGP 

       130        140        150        160        170        180 
EGFWPRRLRL WGGADHAPEG FDPTVTVFHV YDILEHVEHA YSMRAAQLHE RFMDAITPAG 

       190        200        210        220        230        240 
TVITLLGLTP EGHRVAVHVY GTRQYFYMNK AEVDRHLQCR APRDLCERLA AALRESPGAS 

       250        260        270        280        290        300 
FRGISADHFE AEVVERADVY YYETRPTLYY RVFVRSGRAL AYLCDNFCPA IRKYEGGVDA 

       310        320        330        340        350        360 
TTRFILDNPG FVTFGWYRLK PGRGNAPAQP RPPTAFGTSS DVEFNCTADN LAVEGAMCDL 

       370        380        390        400        410        420 
PAYKLMCFDI ECKAGGEDEL AFPVAERPED LVIQISCLLY DLSTTALEHI LLFSLGSCDL 

       430        440        450        460        470        480 
PESHLSDLAS RGLPAPVVLE FDSEFEMLLA FMTFVKQYGP EFVTGYNIIN FDWPFVLTKL 

       490        500        510        520        530        540 
TEIYKVPLDG YGRMNGRGVF RVWDIGQSHF QKRSKIKVNG MVNIDMYGII TDKVKLSSYK 

       550        560        570        580        590        600 
LNAVAEAVLK DKKKDLSYRD IPAYYASGPA QRGVIGEYCV QDSLLVGQLF FKFLPHLELS 

       610        620        630        640        650        660 
AVARLAGINI TRTIYDGQQI RVFTCLLRLA GQKGFILPDT QGRFRGLDKE APKRPAVPRG 

       670        680        690        700        710        720 
EGERPGDGNG DEDKDDDEDG DEDGDEREEV ARETGGRHVG YQGARVLDPT SGFHVDPVVV 

       730        740        750        760        770        780 
FDFASLYPSI IQAHNLCFST LSLRPEAVAH LEADRDYLEI EVGGRRLFFV KAHVRESLLS 

       790        800        810        820        830        840 
ILLRDWLAMR KQIRSRIPQS PPEEAVLLDK QQAAIKVVCN SVYGFTGVQH GLLPCLHVAA 

       850        860        870        880        890        900 
TVTTIGREML LATRAYVHAR WAEFDQLLAD FPEAAGMRAP GPYSMRIIYG DTDSIFVLCR 

       910        920        930        940        950        960 
GLTGEALVAM GDKMASHISR ALFLPPIKLE CEKTFTKLLL IAKKKYIGVI CGGKMLIKGV 

       970        980        990       1000       1010       1020 
DLVRKNNCAF INRTSRALVD LLFYDDTVSG AAAALAERPA EEWLARPLPE GLQAFGAVLV 

      1030       1040       1050       1060       1070       1080 
DAHRRITDPE RDIQDFVLTA ELSRHPRAYT NKRLAHLTVY YKLMARRAQV PSIKDRIPYV 

      1090       1100       1110       1120       1130       1140 
IVAQTREVEE TVARLAALRE LDAAAPGDEP APPAALPSPA KRPRETPSHA DPPGGASKPR 

      1150       1160       1170       1180       1190       1200 
KLLVSELAED PGYAIARGVP LNTDYYFSHL LGAACVTFKA LFGNNAKITE SLLKRFIPET 

      1210       1220       1230       1240 
WHPPDDVAAR LRAAGFGPAG AGATAEETRR MLHRAFDTLA 

« Hide

References

[1]"Nucleotide sequence of the DNA polymerase gene of herpes simplex virus type 2 and comparison with the type 1 counterpart."
Tsurumi T., Maeno K., Nishiyama Y.
Gene 52:129-137(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16321 Genomic DNA. Translation: AAA45853.1.
PIRDJBE21. A27315.

3D structure databases

ProteinModelPortalP07918.
SMRP07918. Positions 61-640, 1205-1240.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR021639. DNAPolymera_Pol_C.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
PF11590. DNAPolymera_Pol. 1 hit.
[Graphical view]
PRINTSPR00106. DNAPOLB.
SMARTSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 2 hits.
PROSITEPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDPOL_HHV21
AccessionPrimary (citable) accession number: P07918
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families