P07918 (DPOL_HHV21) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase catalytic subunit
EC=2.7.7.7
EC=3.1.26.4

Gene names

ORF Names:

UL30

Organism

Human herpesvirus 2 (strain 186) (HHV-2) (Human herpes simplex virus 2)

Taxonomic identifier

10312 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 2

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	1240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Replicates viral genomic DNA. The replication complex is composed of six viral proteins: the DNA polymerase, processivity factor, primase, primase-associated factor, helicase, and ssDNA-binding protein. Additionally, the polymerase contains an intrinsic ribonuclease H (RNase H) activity that specifically degrades RNA/DNA heteroduplexes or duplex DNA substrates in the 5' to 3' direction. Therefore, it can catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during viral DNA replication By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Endonucleolytic cleavage to 5'-phosphomonoester.
Subunit structure	Forms a complex with the ssDNA-binding protein UL29, the DNA polymerase processivity factor, and the alkaline exonuclease. Interacts with the putative helicase-primase complex subunit UL8; this interaction may coordinate leading and lagging strand DNA synthesis at the replication fork By similarity.
Subcellular location	Host nucleus By similarity. Note: The protein is present at discrete sites in nuclei, called replication compartments where viral DNA replication occurs By similarity.
Sequence similarities	Belongs to the DNA polymerase type-B family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Host nucleus
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Endonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1240	1240	DNA polymerase catalytic subunit		PRO_0000046515
Regions
	Compositional bias	991 – 996	6	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	P07918 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 1CDA4B1D11F8180E	FASTA	1,240	137,356
	10 20 30 40 50 60 MFCAAGGPAS PGGKSAARAA SGFFAPHNPR GATQTAPPPC RRQNFYNPHL AQTGTQPKAP 70 80 90 100 110 120 GPAQRHTYYS ECDEFRFIAP RSLDEDAPAE QRTGVHDGRL RRAPKVYCGG DERDVLRVGP 130 140 150 160 170 180 EGFWPRRLRL WGGADHAPEG FDPTVTVFHV YDILEHVEHA YSMRAAQLHE RFMDAITPAG 190 200 210 220 230 240 TVITLLGLTP EGHRVAVHVY GTRQYFYMNK AEVDRHLQCR APRDLCERLA AALRESPGAS 250 260 270 280 290 300 FRGISADHFE AEVVERADVY YYETRPTLYY RVFVRSGRAL AYLCDNFCPA IRKYEGGVDA 310 320 330 340 350 360 TTRFILDNPG FVTFGWYRLK PGRGNAPAQP RPPTAFGTSS DVEFNCTADN LAVEGAMCDL 370 380 390 400 410 420 PAYKLMCFDI ECKAGGEDEL AFPVAERPED LVIQISCLLY DLSTTALEHI LLFSLGSCDL 430 440 450 460 470 480 PESHLSDLAS RGLPAPVVLE FDSEFEMLLA FMTFVKQYGP EFVTGYNIIN FDWPFVLTKL 490 500 510 520 530 540 TEIYKVPLDG YGRMNGRGVF RVWDIGQSHF QKRSKIKVNG MVNIDMYGII TDKVKLSSYK 550 560 570 580 590 600 LNAVAEAVLK DKKKDLSYRD IPAYYASGPA QRGVIGEYCV QDSLLVGQLF FKFLPHLELS 610 620 630 640 650 660 AVARLAGINI TRTIYDGQQI RVFTCLLRLA GQKGFILPDT QGRFRGLDKE APKRPAVPRG 670 680 690 700 710 720 EGERPGDGNG DEDKDDDEDG DEDGDEREEV ARETGGRHVG YQGARVLDPT SGFHVDPVVV 730 740 750 760 770 780 FDFASLYPSI IQAHNLCFST LSLRPEAVAH LEADRDYLEI EVGGRRLFFV KAHVRESLLS 790 800 810 820 830 840 ILLRDWLAMR KQIRSRIPQS PPEEAVLLDK QQAAIKVVCN SVYGFTGVQH GLLPCLHVAA 850 860 870 880 890 900 TVTTIGREML LATRAYVHAR WAEFDQLLAD FPEAAGMRAP GPYSMRIIYG DTDSIFVLCR 910 920 930 940 950 960 GLTGEALVAM GDKMASHISR ALFLPPIKLE CEKTFTKLLL IAKKKYIGVI CGGKMLIKGV 970 980 990 1000 1010 1020 DLVRKNNCAF INRTSRALVD LLFYDDTVSG AAAALAERPA EEWLARPLPE GLQAFGAVLV 1030 1040 1050 1060 1070 1080 DAHRRITDPE RDIQDFVLTA ELSRHPRAYT NKRLAHLTVY YKLMARRAQV PSIKDRIPYV 1090 1100 1110 1120 1130 1140 IVAQTREVEE TVARLAALRE LDAAAPGDEP APPAALPSPA KRPRETPSHA DPPGGASKPR 1150 1160 1170 1180 1190 1200 KLLVSELAED PGYAIARGVP LNTDYYFSHL LGAACVTFKA LFGNNAKITE SLLKRFIPET 1210 1220 1230 1240 WHPPDDVAAR LRAAGFGPAG AGATAEETRR MLHRAFDTLA

References

[1]	"Nucleotide sequence of the DNA polymerase gene of herpes simplex virus type 2 and comparison with the type 1 counterpart." Tsurumi T., Maeno K., Nishiyama Y. Gene 52:129-137(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16321 Genomic DNA. Translation: AAA45853.1.
PIR	DJBE21. A27315.
3D structure databases
ProteinModelPortal	P07918.
SMR	P07918. Positions 61-640, 1205-1240.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.90.1600.10. 2 hits.
InterPro	IPR006172. DNA-dir_DNA_pol_B. IPR017964. DNA-dir_DNA_pol_B_CS. IPR006133. DNA-dir_DNA_pol_B_exonuc. IPR006134. DNA-dir_DNA_pol_B_multi_dom. IPR023211. DNA_pol_palm_dom. IPR021639. DNAPolymera_Pol_C. IPR012337. RNaseH-like_dom. [Graphical view]
Pfam	PF00136. DNA_pol_B. 1 hit. PF03104. DNA_pol_B_exo1. 1 hit. PF11590. DNAPolymera_Pol. 1 hit. [Graphical view]
PRINTS	PR00106. DNAPOLB.
SMART	SM00486. POLBc. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS00116. DNA_POLYMERASE_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPOL_HHV21

Accession

Primary (citable) accession number: P07918

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program