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P07918

- DPOL_HHV21

UniProt

P07918 - DPOL_HHV21

Protein

DNA polymerase catalytic subunit

Gene

UL30

Organism
Human herpesvirus 2 (strain 186) (HHV-2) (Human herpes simplex virus 2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Replicates viral genomic DNA. The replication complex is composed of six viral proteins: the DNA polymerase, processivity factor, primase, primase-associated factor, helicase, and ssDNA-binding protein. Additionally, the polymerase contains an intrinsic ribonuclease H (RNase H) activity that specifically degrades RNA/DNA heteroduplexes or duplex DNA substrates in the 5' to 3' direction. Therefore, it can catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during viral DNA replication By similarity.By similarity

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
    Endonucleolytic cleavage to 5'-phosphomonoester.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. nucleotide binding Source: InterPro
    4. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase catalytic subunit (EC:2.7.7.7, EC:3.1.26.4)
    Gene namesi
    ORF Names:UL30
    OrganismiHuman herpesvirus 2 (strain 186) (HHV-2) (Human herpes simplex virus 2)
    Taxonomic identifieri10312 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Subcellular locationi

    Host nucleus By similarity
    Note: The protein is present at discrete sites in nuclei, called replication compartments where viral DNA replication occurs.By similarity

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12401240DNA polymerase catalytic subunitPRO_0000046515Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a complex with the ssDNA-binding protein UL29, the DNA polymerase processivity factor, and the alkaline exonuclease. Interacts with the putative helicase-primase complex subunit UL8; this interaction may coordinate leading and lagging strand DNA synthesis at the replication fork By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP07918.
    SMRiP07918. Positions 61-640, 1205-1240.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi991 – 9966Poly-Ala

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B family.Curated

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    3.90.1600.10. 2 hits.
    InterProiIPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR021639. DNAPolymera_Pol_C.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 1 hit.
    PF11590. DNAPolymera_Pol. 1 hit.
    [Graphical view]
    PRINTSiPR00106. DNAPOLB.
    SMARTiSM00486. POLBc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 2 hits.
    PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07918-1 [UniParc]FASTAAdd to Basket

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    MFCAAGGPAS PGGKSAARAA SGFFAPHNPR GATQTAPPPC RRQNFYNPHL     50
    AQTGTQPKAP GPAQRHTYYS ECDEFRFIAP RSLDEDAPAE QRTGVHDGRL 100
    RRAPKVYCGG DERDVLRVGP EGFWPRRLRL WGGADHAPEG FDPTVTVFHV 150
    YDILEHVEHA YSMRAAQLHE RFMDAITPAG TVITLLGLTP EGHRVAVHVY 200
    GTRQYFYMNK AEVDRHLQCR APRDLCERLA AALRESPGAS FRGISADHFE 250
    AEVVERADVY YYETRPTLYY RVFVRSGRAL AYLCDNFCPA IRKYEGGVDA 300
    TTRFILDNPG FVTFGWYRLK PGRGNAPAQP RPPTAFGTSS DVEFNCTADN 350
    LAVEGAMCDL PAYKLMCFDI ECKAGGEDEL AFPVAERPED LVIQISCLLY 400
    DLSTTALEHI LLFSLGSCDL PESHLSDLAS RGLPAPVVLE FDSEFEMLLA 450
    FMTFVKQYGP EFVTGYNIIN FDWPFVLTKL TEIYKVPLDG YGRMNGRGVF 500
    RVWDIGQSHF QKRSKIKVNG MVNIDMYGII TDKVKLSSYK LNAVAEAVLK 550
    DKKKDLSYRD IPAYYASGPA QRGVIGEYCV QDSLLVGQLF FKFLPHLELS 600
    AVARLAGINI TRTIYDGQQI RVFTCLLRLA GQKGFILPDT QGRFRGLDKE 650
    APKRPAVPRG EGERPGDGNG DEDKDDDEDG DEDGDEREEV ARETGGRHVG 700
    YQGARVLDPT SGFHVDPVVV FDFASLYPSI IQAHNLCFST LSLRPEAVAH 750
    LEADRDYLEI EVGGRRLFFV KAHVRESLLS ILLRDWLAMR KQIRSRIPQS 800
    PPEEAVLLDK QQAAIKVVCN SVYGFTGVQH GLLPCLHVAA TVTTIGREML 850
    LATRAYVHAR WAEFDQLLAD FPEAAGMRAP GPYSMRIIYG DTDSIFVLCR 900
    GLTGEALVAM GDKMASHISR ALFLPPIKLE CEKTFTKLLL IAKKKYIGVI 950
    CGGKMLIKGV DLVRKNNCAF INRTSRALVD LLFYDDTVSG AAAALAERPA 1000
    EEWLARPLPE GLQAFGAVLV DAHRRITDPE RDIQDFVLTA ELSRHPRAYT 1050
    NKRLAHLTVY YKLMARRAQV PSIKDRIPYV IVAQTREVEE TVARLAALRE 1100
    LDAAAPGDEP APPAALPSPA KRPRETPSHA DPPGGASKPR KLLVSELAED 1150
    PGYAIARGVP LNTDYYFSHL LGAACVTFKA LFGNNAKITE SLLKRFIPET 1200
    WHPPDDVAAR LRAAGFGPAG AGATAEETRR MLHRAFDTLA 1240
    Length:1,240
    Mass (Da):137,356
    Last modified:August 1, 1988 - v1
    Checksum:i1CDA4B1D11F8180E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16321 Genomic DNA. Translation: AAA45853.1.
    PIRiA27315. DJBE21.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16321 Genomic DNA. Translation: AAA45853.1 .
    PIRi A27315. DJBE21.

    3D structure databases

    ProteinModelPortali P07918.
    SMRi P07918. Positions 61-640, 1205-1240.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    3.90.1600.10. 2 hits.
    InterProi IPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR021639. DNAPolymera_Pol_C.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 1 hit.
    PF11590. DNAPolymera_Pol. 1 hit.
    [Graphical view ]
    PRINTSi PR00106. DNAPOLB.
    SMARTi SM00486. POLBc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 2 hits.
    PROSITEi PS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the DNA polymerase gene of herpes simplex virus type 2 and comparison with the type 1 counterpart."
      Tsurumi T., Maeno K., Nishiyama Y.
      Gene 52:129-137(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiDPOL_HHV21
    AccessioniPrimary (citable) accession number: P07918
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3