P07895 (SODM_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Superoxide dismutase [Mn], mitochondrial
EC=1.15.1.1

Gene names

Name:

Sod2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Ref.5
Catalytic activity	2 superoxide + 2 H+ = O2 + H2O2.
Cofactor	Binds 1 manganese ion per subunit By similarity.
Subunit structure	Homotetramer.
Subcellular location	Mitochondrion matrix.
Post-translational modification	Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
Sequence similarities	Belongs to the iron/manganese superoxide dismutase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Manganese Metal-binding
Molecular function	Oxidoreductase
PTM	Acetylation Nitration
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	age-dependent response to reactive oxygen species Inferred from sequence or structural similarity. Source: UniProtKB cellular response to ethanol Inferred from expression pattern PubMed 19703426. Source: RGD detection of oxygen Inferred from electronic annotation. Source: Compara erythrophore differentiation Inferred from electronic annotation. Source: Compara glutathione metabolic process Inferred from electronic annotation. Source: Compara heart development Inferred from electronic annotation. Source: Compara hemopoiesis Inferred from electronic annotation. Source: Compara hydrogen peroxide biosynthetic process Inferred from direct assay PubMed 15642720. Source: RGD iron ion homeostasis Inferred from electronic annotation. Source: Compara liver development Inferred from electronic annotation. Source: Compara locomotory behavior Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from mutant phenotype PubMed 11961082. Source: RGD negative regulation of fat cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of fibroblast proliferation Inferred from electronic annotation. Source: Compara negative regulation of neuron apoptotic process Inferred from electronic annotation. Source: Compara neuron development Inferred from electronic annotation. Source: Compara oxygen homeostasis Inferred from electronic annotation. Source: Compara positive regulation of nitric oxide biosynthetic process Inferred from electronic annotation. Source: Compara post-embryonic development Inferred from electronic annotation. Source: Compara protein homooligomerization Inferred from direct assay PubMed 19384983. Source: RGD protein homotetramerization Inferred from electronic annotation. Source: Compara regulation of catalytic activity Inferred from electronic annotation. Source: Compara regulation of mitochondrial membrane potential Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB release of cytochrome c from mitochondria Inferred from electronic annotation. Source: Compara removal of superoxide radicals Inferred from direct assay PubMed 12107766PubMed 15642720PubMed 19439219. Source: RGD respiratory electron transport chain Inferred from electronic annotation. Source: Compara response to L-ascorbic acid Inferred from expression pattern PubMed 20122981. Source: RGD response to activity Inferred from expression pattern PubMed 19297546. Source: RGD response to axon injury Inferred from electronic annotation. Source: Compara response to cadmium ion Inferred from expression pattern PubMed 20107868. Source: RGD response to drug Inferred from direct assay PubMed 15959853. Source: RGD response to electrical stimulus Inferred from expression pattern PubMed 17962976. Source: RGD response to gamma radiation Inferred from electronic annotation. Source: Compara response to hydrogen peroxide Inferred from expression pattern PubMed 19917063. Source: RGD response to hyperoxia Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from expression pattern PubMed 16911942. Source: RGD response to lipopolysaccharide Inferred from expression pattern PubMed 17804075. Source: RGD response to manganese ion Inferred from expression pattern PubMed 17920449. Source: RGD response to selenium ion Inferred from expression pattern PubMed 17804075. Source: RGD response to silicon dioxide Inferred from expression pattern PubMed 17575405. Source: RGD response to zinc ion Inferred from expression pattern PubMed 20128680. Source: RGD superoxide anion generation Inferred from electronic annotation. Source: Compara vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: Compara mitochondrial nucleoid Inferred from direct assay PubMed 19228881. Source: RGD
Molecular_function	DNA binding Inferred from direct assay PubMed 19228881. Source: RGD identical protein binding Inferred from direct assay PubMed 19384983. Source: RGD manganese ion binding Inferred from sequence or structural similarity. Source: UniProtKB oxygen binding Inferred from direct assay PubMed 12107766PubMed 15642720. Source: RGD superoxide dismutase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion
	Chain	25 – 222	198	Superoxide dismutase [Mn], mitochondrial		PRO_0000032874
Sites
	Metal binding	50	1	Manganese By similarity
	Metal binding	98	1	Manganese By similarity
	Metal binding	183	1	Manganese By similarity
	Metal binding	187	1	Manganese By similarity
Amino acid modifications
	Modified residue	58	1	Nitrated tyrosine Ref.6
	Modified residue	68	1	N6-acetyllysine By similarity
	Modified residue	130	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	167	1	Q → H in CAA68549. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07895 [UniParc]. Last modified May 1, 1992. Version 2. Checksum: 8CCC1E0E857B3138	FASTA	222	24,674
	10 20 30 40 50 60 MLCRAACSAG RRLGPAASTA GSRHKHSLPD LPYDYGALEP HINAQIMQLH HSKHHATYVN 70 80 90 100 110 120 NLNVTEEKYH EALAKGDVTT QVALQPALKF NGGGHINHSI FWTNLSPKGG GEPKGELLEA 130 140 150 160 170 180 IKRDFGSFEK FKEKLTAVSV GVQGSGWGWL GFNKEQGRLQ IAACSNQDPL QGTTGLIPLL 190 200 210 220 GIDVWEHAYY LQYKNVRPDY LKAIWNVINW ENVSQRYIVC KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequences of cDNAs coding for rat manganese-containing superoxide dismutase." Ho Y.-S., Crapo J.D. Nucleic Acids Res. 15:10070-10070(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Molecular structure of a functional rat gene for manganese-containing superoxide dismutase." Ho Y.-S., Howard A.J., Crapo J.D. Am. J. Respir. Cell Mol. Biol. 4:278-286(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[4]	Lubec G., Afjehi-Sadat L., Diao W., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 54-68; 76-108; 135-154 AND 203-216, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain, Hippocampus and Spinal cord.
[5]	"Quantitative assessment of tyrosine nitration of manganese superoxide dismutase in angiotensin II-infused rat kidney." Guo W., Adachi T., Matsui R., Xu S., Jiang B., Zou M.H., Kirber M., Lieberthal W., Cohen R.A. Am. J. Physiol. 285:H1396-H1403(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NITRATION, FUNCTION DURING OXIDATIVE STRESS.
[6]	"Detection of sequence-specific tyrosine nitration of manganese SOD and SERCA in cardiovascular disease and aging." Xu S., Ying J., Jiang B., Guo W., Adachi T., Sharov V., Lazar H., Menzoian J., Knyushko T.V., Bigelow D., Schoeneich C., Cohen R.A. Am. J. Physiol. 290:H2220-H2227(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NITRATION AT TYR-58.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00497 mRNA. Translation: CAA68549.1. X56600 Genomic DNA. Translation: CAA39937.1. BC070913 mRNA. Translation: AAH70913.1.
IPI	IPI00211593.
PIR	DSRTN. S21661.
RefSeq	NP_058747.1. NM_017051.2.
UniGene	Rn.10488.
3D structure databases
ProteinModelPortal	P07895.
SMR	P07895. Positions 25-222.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07895. 1 interaction.
PTM databases
PhosphoSite	P07895.
2D gel databases
World-2DPAGE	0004:P07895.
Proteomic databases
PaxDb	P07895.
PRIDE	P07895.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025794; ENSRNOP00000025794; ENSRNOG00000019048.
GeneID	24787.
KEGG	rno:24787.
Organism-specific databases
CTD	6648.
RGD	3732. Sod2.
Phylogenomic databases
eggNOG	COG0605.
GeneTree	ENSGT00390000011877.
HOGENOM	HOG000013583.
HOVERGEN	HBG004451.
InParanoid	P07895.
KO	K04564.
OMA	MAPPGKG.
OrthoDB	EOG4R503R.
Gene expression databases
ArrayExpress	P07895.
Genevestigator	P07895.
GermOnline	ENSRNOG00000019048. Rattus norvegicus.
Family and domain databases
InterPro	IPR001189. Mn/Fe_SOD. IPR019833. Mn/Fe_SOD_BS. IPR019832. Mn/Fe_SOD_C. IPR019831. Mn/Fe_SOD_N. [Graphical view]
PANTHER	PTHR11404. PTHR11404. 1 hit.
Pfam	PF02777. Sod_Fe_C. 1 hit. PF00081. Sod_Fe_N. 1 hit. [Graphical view]
PIRSF	PIRSF000349. SODismutase. 1 hit.
PRINTS	PR01703. MNSODISMTASE.
SUPFAM	SSF46609. SODismutase. 1 hit. SSF54719. SODismutase. 1 hit.
PROSITE	PS00088. SOD_MN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604412.

Entry information

Entry name

SODM_RAT

Accession

Primary (citable) accession number: P07895

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program