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Protein

Extracellular agarase

Gene

dagA

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551NucleophilePROSITE-ProRule annotation
Active sitei160 – 1601Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular agarase (EC:3.2.1.81)
Gene namesi
Name:dagA
Ordered Locus Names:SCO3471
ORF Names:SCE65.07c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Tat-type signalPROSITE-ProRule annotation1 PublicationAdd
BLAST
Chaini31 – 309279Extracellular agarasePRO_0000011797Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO3471.

Structurei

3D structure databases

ProteinModelPortaliP07883.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiP07883.
KOiK01219.
OMAiHLEYYID.
OrthoDBiEOG6T1WW5.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR016287. Agarase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PIRSFiPIRSF001097. Agarase. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNRRDLIKW SAVALGAGAG LAGPAPAAHA ADLEWEQYPV PAAPGGNRSW
60 70 80 90 100
QLLPSHSDDF NYTGKPQTFR GRWLDQHKDG WSGPANSLYS ARHSWVADGN
110 120 130 140 150
LIVEGRRAPD GRVYCGYVTS RTPVEYPLYT EVLMRVSGLK LSSNFWLLSR
160 170 180 190 200
DDVNEIDVIE CYGNESLHGK HMNTAYHIFQ RNPFTELARS QKGYFADGSY
210 220 230 240 250
GYNGETGQVF GDGAGQPLLR NGFHRYGVHW ISATEFDFYF NGRLVRRLNR
260 270 280 290 300
SNDLRDPRSR FFDQPMHLIL NTESHQWRVD RGIEPTDAEL ADPSINNIYY

RWVRTYQAV
Length:309
Mass (Da):35,164
Last modified:July 1, 1989 - v2
Checksum:i18E479E642DB1A43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05811 Genomic DNA. Translation: CAA29257.1.
AL939116 Genomic DNA. Translation: CAB61795.1.
PIRiS07322. EUSMAG.
RefSeqiNP_627674.1. NC_003888.3.
WP_011029010.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB61795; CAB61795; CAB61795.
GeneIDi1098908.
KEGGisco:SCO3471.
PATRICi23736692. VBIStrCoe124346_3531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05811 Genomic DNA. Translation: CAA29257.1.
AL939116 Genomic DNA. Translation: CAB61795.1.
PIRiS07322. EUSMAG.
RefSeqiNP_627674.1. NC_003888.3.
WP_011029010.1. NC_003888.3.

3D structure databases

ProteinModelPortaliP07883.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO3471.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB61795; CAB61795; CAB61795.
GeneIDi1098908.
KEGGisco:SCO3471.
PATRICi23736692. VBIStrCoe124346_3531.

Phylogenomic databases

InParanoidiP07883.
KOiK01219.
OMAiHLEYYID.
OrthoDBiEOG6T1WW5.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR016287. Agarase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PIRSFiPIRSF001097. Agarase. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis."
    Buttner M.J., Fearnley I.M., Bibb M.J.
    Mol. Gen. Genet. 209:101-109(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-61.
    Strain: A3(2) / NRRL B-16638.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiAGAR_STRCO
AccessioniPrimary (citable) accession number: P07883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 1, 1989
Last modified: July 22, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.