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P07883

- AGAR_STRCO

UniProt

P07883 - AGAR_STRCO

Protein

Extracellular agarase

Gene

dagA

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei155 – 1551NucleophilePROSITE-ProRule annotation
    Active sitei160 – 1601Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-agarase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Extracellular agarase (EC:3.2.1.81)
    Gene namesi
    Name:dagA
    Ordered Locus Names:SCO3471
    ORF Names:SCE65.07c
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Tat-type signal1 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Chaini31 – 309279Extracellular agarasePRO_0000011797Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

    Interactioni

    Protein-protein interaction databases

    STRINGi100226.SCO3471.

    Structurei

    3D structure databases

    ProteinModelPortaliP07883.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    KOiK01219.
    OMAiHWISATE.
    OrthoDBiEOG6T1WW5.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR016287. Agarase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001097. Agarase. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07883-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVNRRDLIKW SAVALGAGAG LAGPAPAAHA ADLEWEQYPV PAAPGGNRSW    50
    QLLPSHSDDF NYTGKPQTFR GRWLDQHKDG WSGPANSLYS ARHSWVADGN 100
    LIVEGRRAPD GRVYCGYVTS RTPVEYPLYT EVLMRVSGLK LSSNFWLLSR 150
    DDVNEIDVIE CYGNESLHGK HMNTAYHIFQ RNPFTELARS QKGYFADGSY 200
    GYNGETGQVF GDGAGQPLLR NGFHRYGVHW ISATEFDFYF NGRLVRRLNR 250
    SNDLRDPRSR FFDQPMHLIL NTESHQWRVD RGIEPTDAEL ADPSINNIYY 300
    RWVRTYQAV 309
    Length:309
    Mass (Da):35,164
    Last modified:July 1, 1989 - v2
    Checksum:i18E479E642DB1A43
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05811 Genomic DNA. Translation: CAA29257.1.
    AL939116 Genomic DNA. Translation: CAB61795.1.
    PIRiS07322. EUSMAG.
    RefSeqiNP_627674.1. NC_003888.3.
    WP_011029010.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB61795; CAB61795; CAB61795.
    GeneIDi1098908.
    KEGGisco:SCO3471.
    PATRICi23736692. VBIStrCoe124346_3531.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05811 Genomic DNA. Translation: CAA29257.1 .
    AL939116 Genomic DNA. Translation: CAB61795.1 .
    PIRi S07322. EUSMAG.
    RefSeqi NP_627674.1. NC_003888.3.
    WP_011029010.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali P07883.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO3471.

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB61795 ; CAB61795 ; CAB61795 .
    GeneIDi 1098908.
    KEGGi sco:SCO3471.
    PATRICi 23736692. VBIStrCoe124346_3531.

    Phylogenomic databases

    KOi K01219.
    OMAi HWISATE.
    OrthoDBi EOG6T1WW5.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR016287. Agarase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001097. Agarase. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis."
      Buttner M.J., Fearnley I.M., Bibb M.J.
      Mol. Gen. Genet. 209:101-109(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-61.
      Strain: A3(2) / NRRL B-16638.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.

    Entry informationi

    Entry nameiAGAR_STRCO
    AccessioniPrimary (citable) accession number: P07883
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3