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P07883

- AGAR_STRCO

UniProt

P07883 - AGAR_STRCO

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Protein

Extracellular agarase

Gene

dagA

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551NucleophilePROSITE-ProRule annotation
Active sitei160 – 1601Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-agarase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular agarase (EC:3.2.1.81)
Gene namesi
Name:dagA
Ordered Locus Names:SCO3471
ORF Names:SCE65.07c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Tat-type signal1 PublicationPROSITE-ProRule annotationAdd
BLAST
Chaini31 – 309279Extracellular agarasePRO_0000011797Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO3471.

Structurei

3D structure databases

ProteinModelPortaliP07883.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiP07883.
KOiK01219.
OMAiHWISATE.
OrthoDBiEOG6T1WW5.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR016287. Agarase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PIRSFiPIRSF001097. Agarase. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07883-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNRRDLIKW SAVALGAGAG LAGPAPAAHA ADLEWEQYPV PAAPGGNRSW
60 70 80 90 100
QLLPSHSDDF NYTGKPQTFR GRWLDQHKDG WSGPANSLYS ARHSWVADGN
110 120 130 140 150
LIVEGRRAPD GRVYCGYVTS RTPVEYPLYT EVLMRVSGLK LSSNFWLLSR
160 170 180 190 200
DDVNEIDVIE CYGNESLHGK HMNTAYHIFQ RNPFTELARS QKGYFADGSY
210 220 230 240 250
GYNGETGQVF GDGAGQPLLR NGFHRYGVHW ISATEFDFYF NGRLVRRLNR
260 270 280 290 300
SNDLRDPRSR FFDQPMHLIL NTESHQWRVD RGIEPTDAEL ADPSINNIYY

RWVRTYQAV
Length:309
Mass (Da):35,164
Last modified:July 1, 1989 - v2
Checksum:i18E479E642DB1A43
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05811 Genomic DNA. Translation: CAA29257.1.
AL939116 Genomic DNA. Translation: CAB61795.1.
PIRiS07322. EUSMAG.
RefSeqiNP_627674.1. NC_003888.3.
WP_011029010.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB61795; CAB61795; CAB61795.
GeneIDi1098908.
KEGGisco:SCO3471.
PATRICi23736692. VBIStrCoe124346_3531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05811 Genomic DNA. Translation: CAA29257.1 .
AL939116 Genomic DNA. Translation: CAB61795.1 .
PIRi S07322. EUSMAG.
RefSeqi NP_627674.1. NC_003888.3.
WP_011029010.1. NC_003888.3.

3D structure databases

ProteinModelPortali P07883.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO3471.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB61795 ; CAB61795 ; CAB61795 .
GeneIDi 1098908.
KEGGi sco:SCO3471.
PATRICi 23736692. VBIStrCoe124346_3531.

Phylogenomic databases

InParanoidi P07883.
KOi K01219.
OMAi HWISATE.
OrthoDBi EOG6T1WW5.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR016287. Agarase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
[Graphical view ]
PIRSFi PIRSF001097. Agarase. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis."
    Buttner M.J., Fearnley I.M., Bibb M.J.
    Mol. Gen. Genet. 209:101-109(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-61.
    Strain: A3(2) / NRRL B-16638.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiAGAR_STRCO
AccessioniPrimary (citable) accession number: P07883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3