Reviewed, UniProtKB/Swiss-Prot P07883 (AGAR_STRCO)

Names and origin

Protein names

Recommended name:
    Extracellular agarase
    EC=3.2.1.81

Gene names

Name:	dagA
Ordered Locus Names:	SCO3471
ORF Names:	SCE65.07c

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.
Subcellular location	Secreted.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-agarase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Tat-type signal Ref.1
	Chain	31 – 309	279	Extracellular agarase		PRO_0000011797
Sites
	Active site	155	1	Nucleophile By similarity
	Active site	160	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07883-1 [UniParc]. Last modified July 1, 1989. Version 2. Checksum: 18E479E642DB1A43	FASTA	309	35,164
	10 20 30 40 50 60 MVNRRDLIKW SAVALGAGAG LAGPAPAAHA ADLEWEQYPV PAAPGGNRSW QLLPSHSDDF 70 80 90 100 110 120 NYTGKPQTFR GRWLDQHKDG WSGPANSLYS ARHSWVADGN LIVEGRRAPD GRVYCGYVTS 130 140 150 160 170 180 RTPVEYPLYT EVLMRVSGLK LSSNFWLLSR DDVNEIDVIE CYGNESLHGK HMNTAYHIFQ 190 200 210 220 230 240 RNPFTELARS QKGYFADGSY GYNGETGQVF GDGAGQPLLR NGFHRYGVHW ISATEFDFYF 250 260 270 280 290 300 NGRLVRRLNR SNDLRDPRSR FFDQPMHLIL NTESHQWRVD RGIEPTDAEL ADPSINNIYY RWVRTYQAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis." Buttner M.J., Fearnley I.M., Bibb M.J. Mol. Gen. Genet. 209:101-109(1987) [PubMed: 17165236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-61. Strain: A3(2).
[2]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X05811 Genomic DNA. Translation: CAA29257.1. AL939116 Genomic DNA. Translation: CAB61795.1.
PIR	EUSMAG. S07322.
RefSeq	NP_627674.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Genome annotation databases
GeneID	1098908.
GenomeReviews	Gene locus SCO3471 in contig AL645882_GR.
KEGG	sco:SCO3471.
NMPDR	fig|100226.1.peg.3431.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	SCOE100226:SCO3471-MON.
BRENDA	3.2.1.81. 1084.
Family and domain databases
InterPro	IPR016287. Agarase. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
PIRSF	PIRSF001097. Agarase. 1 hit.
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGAR_STRCO

Accession

Primary (citable) accession number: P07883

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	July 1, 1989
Last modified:	September 22, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)