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P07874

- ALGA_PSEAE

UniProt

P07874 - ALGA_PSEAE

Protein

Alginate biosynthesis protein AlgA

Gene

algA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (11 Jan 2001)
      Previous versions | rss
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    Functioni

    Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics.1 Publication

    Catalytic activityi

    D-mannose 6-phosphate = D-fructose 6-phosphate.1 Publication
    GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.1 Publication

    Cofactori

    Cobalt (for PMI).1 Publication

    Kineticsi

    1. KM=3.03 mM for D-mannose 6-phosphate1 Publication
    2. KM=20.5 µM for D-mannose 1-phosphate1 Publication
    3. KM=29.5 µM for GTP1 Publication
    4. KM=14.2 µM for GDP-D-mannose1 Publication

    Vmax=830 nmol/min/mg enzyme for the PMI forward reaction1 Publication

    Vmax=5680 nmol/min/mg enzyme for the GMP forward reaction1 Publication

    Vmax=5170 nmol/min/mg enzyme for the GMP reverse reaction1 Publication

    pH dependencei

    Optimum pH is 7.0 for PMI activity, and 7.6 for GMP activity.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. mannose-1-phosphate guanylyltransferase activity Source: PseudoCAP
    3. mannose-6-phosphate isomerase activity Source: PseudoCAP

    GO - Biological processi

    1. alginic acid biosynthetic process Source: PseudoCAP
    2. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Alginate biosynthesis

    Keywords - Ligandi

    Cobalt, GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13384.
    BRENDAi2.7.7.13. 5087.
    SABIO-RKP07874.
    UniPathwayiUPA00126; UER00423.
    UPA00126; UER00930.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alginate biosynthesis protein AlgA
    Including the following 2 domains:
    Mannose-6-phosphate isomerase (EC:5.3.1.8)
    Alternative name(s):
    Phosphohexomutase
    Phosphomannose isomerase
    Short name:
    PMI
    Mannose-1-phosphate guanylyltransferase (EC:2.7.7.13)
    Alternative name(s):
    GDP-mannose pyrophosphorylase
    Short name:
    GMP
    Short name:
    GMPP
    GTP--mannose-1-phosphate guanylyltransferase
    Gene namesi
    Name:algA
    Synonyms:pmi
    Ordered Locus Names:PA3551
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA3551.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 481481Alginate biosynthesis protein AlgAPRO_0000194248Add
    BLAST

    Proteomic databases

    PRIDEiP07874.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi208964.PA3551.

    Structurei

    3D structure databases

    ProteinModelPortaliP07874.
    SMRiP07874. Positions 1-346.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0662.
    HOGENOMiHOG000153549.
    KOiK16011.
    OMAiDRHQVKR.
    OrthoDBiEOG6X3W6T.
    PhylomeDBiP07874.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    3.90.550.10. 1 hit.
    InterProiIPR006375. Man1P_GuaTrfase/Man6P_Isoase.
    IPR001538. Man6P_isomerase-2_C.
    IPR005835. NTP_transferase.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PfamiPF01050. MannoseP_isomer. 1 hit.
    PF00483. NTP_transferase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51182. SSF51182. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsiTIGR01479. GMP_PMI. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P07874-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIPVILSGGS GSRLWPLSRK QYPKQFLALT GDDTLFQQTI KRLAFDGMQA    50
    PLLVCNKEHR FIVQEQLEAQ NLASQAILLE PFGRNTAPAV AIAAMKLVAE 100
    GRDELLLILP ADHVIEDQRA FQQALALATN AAEKGEMVLF GIPASRPETG 150
    YGYIRASADA QLPEGVSRVQ SFVEKPDEAR AREFVAAGGY YWNSGMFLFR 200
    ASRYLEELKK HDADIYDTCL LALERSQHDG DLVNIDAATF ECCPDNSIDY 250
    AVMEKTSRAC VVPLSAGWND VGSWSSIWDV HAKDANGNVT KGDVLVHDSH 300
    NCLVHGNGKL VSVIGLEDIV VVETKDAMMI AHKDRVQDVK HVVKDLDAQG 350
    RSETQNHCEV YRPWGSYDSV DMGGRFQVKH ITVKPGARLS LQMHHHRAEH 400
    WIVVSGTAQV TCDDKTFLLT ENQSTYIPIA SVHRLANPGK IPLEIIEVQS 450
    GSYLGEDDIE RLEDVYGRTA EPALQVVAGS R 481
    Length:481
    Mass (Da):53,128
    Last modified:January 11, 2001 - v3
    Checksum:i2D497AF9059AEF97
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 272FL → LV in AAA25972. (PubMed:3089876)Curated
    Sequence conflicti115 – 1151I → L in AAA25972. (PubMed:3089876)Curated
    Sequence conflicti157 – 1571S → T in AAA25972. (PubMed:3089876)Curated
    Sequence conflicti359 – 3602EV → DL in AAA25972. (PubMed:3089876)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14037 Genomic DNA. Translation: AAA25972.1.
    AE004091 Genomic DNA. Translation: AAG06939.1.
    PIRiA38598.
    B83201.
    RefSeqiNP_252241.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06939; AAG06939; PA3551.
    GeneIDi879142.
    KEGGipae:PA3551.
    PATRICi19841717. VBIPseAer58763_3716.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14037 Genomic DNA. Translation: AAA25972.1 .
    AE004091 Genomic DNA. Translation: AAG06939.1 .
    PIRi A38598.
    B83201.
    RefSeqi NP_252241.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali P07874.
    SMRi P07874. Positions 1-346.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA3551.

    Proteomic databases

    PRIDEi P07874.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG06939 ; AAG06939 ; PA3551 .
    GeneIDi 879142.
    KEGGi pae:PA3551.
    PATRICi 19841717. VBIPseAer58763_3716.

    Organism-specific databases

    PseudoCAPi PA3551.

    Phylogenomic databases

    eggNOGi COG0662.
    HOGENOMi HOG000153549.
    KOi K16011.
    OMAi DRHQVKR.
    OrthoDBi EOG6X3W6T.
    PhylomeDBi P07874.

    Enzyme and pathway databases

    UniPathwayi UPA00126 ; UER00423 .
    UPA00126 ; UER00930 .
    BioCyci MetaCyc:MONOMER-13384.
    BRENDAi 2.7.7.13. 5087.
    SABIO-RK P07874.

    Family and domain databases

    Gene3Di 2.60.120.10. 1 hit.
    3.90.550.10. 1 hit.
    InterProi IPR006375. Man1P_GuaTrfase/Man6P_Isoase.
    IPR001538. Man6P_isomerase-2_C.
    IPR005835. NTP_transferase.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view ]
    Pfami PF01050. MannoseP_isomer. 1 hit.
    PF00483. NTP_transferase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51182. SSF51182. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsi TIGR01479. GMP_PMI. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence analysis of the phosphomannose isomerase gene (pmi) of Pseudomonas aeruginosa and comparison with the corresponding Escherichia coli gene manA."
      Darzins A., Frantz B., Vanags R.I., Chakrabarty A.M.
      Gene 42:293-302(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    3. "Purification and characterization of phosphomannose isomerase-guanosine diphospho-D-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa."
      Shinabarger D., Berry A., May T.B., Rothmel R., Fialho A., Chakrabarty A.M.
      J. Biol. Chem. 266:2080-2088(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiALGA_PSEAE
    AccessioniPrimary (citable) accession number: P07874
    Secondary accession number(s): Q9HY66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3