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Protein

Alginate biosynthesis protein AlgA

Gene

algA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics.1 Publication

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.1 Publication
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.1 Publication

Cofactori

Co2+1 PublicationNote: Co2+ (for PMI).1 Publication

Kineticsi

  1. KM=3.03 mM for D-mannose 6-phosphate1 Publication
  2. KM=20.5 µM for D-mannose 1-phosphate1 Publication
  3. KM=29.5 µM for GTP1 Publication
  4. KM=14.2 µM for GDP-D-mannose1 Publication

Vmax=830 nmol/min/mg enzyme for the PMI forward reaction1 Publication

Vmax=5680 nmol/min/mg enzyme for the GMP forward reaction1 Publication

Vmax=5170 nmol/min/mg enzyme for the GMP reverse reaction1 Publication

pH dependencei

Optimum pH is 7.0 for PMI activity, and 7.6 for GMP activity.1 Publication

Pathwayi

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mannose-1-phosphate guanylyltransferase activity Source: PseudoCAP
  3. mannose-6-phosphate isomerase activity Source: PseudoCAP

GO - Biological processi

  1. alginic acid biosynthetic process Source: PseudoCAP
  2. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Alginate biosynthesis

Keywords - Ligandi

Cobalt, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13384.
BRENDAi2.7.7.13. 5087.
SABIO-RKP07874.
UniPathwayiUPA00126; UER00423.
UPA00126; UER00930.

Names & Taxonomyi

Protein namesi
Recommended name:
Alginate biosynthesis protein AlgA
Including the following 2 domains:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Mannose-1-phosphate guanylyltransferase (EC:2.7.7.13)
Alternative name(s):
GDP-mannose pyrophosphorylase
Short name:
GMP
Short name:
GMPP
GTP--mannose-1-phosphate guanylyltransferase
Gene namesi
Name:algA
Synonyms:pmi
Ordered Locus Names:PA3551
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3551.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481Alginate biosynthesis protein AlgAPRO_0000194248Add
BLAST

Proteomic databases

PRIDEiP07874.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA3551.

Structurei

3D structure databases

ProteinModelPortaliP07874.
SMRiP07874. Positions 1-346, 362-453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0662.
HOGENOMiHOG000153549.
InParanoidiP07874.
KOiK16011.
OMAiSDLGTWK.
OrthoDBiEOG6X3W6T.
PhylomeDBiP07874.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR006375. Man1P_GuaTrfase/Man6P_Isoase.
IPR001538. Man6P_isomerase-2_C.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF01050. MannoseP_isomer. 1 hit.
PF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01479. GMP_PMI. 1 hit.

Sequencei

Sequence statusi: Complete.

P07874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPVILSGGS GSRLWPLSRK QYPKQFLALT GDDTLFQQTI KRLAFDGMQA
60 70 80 90 100
PLLVCNKEHR FIVQEQLEAQ NLASQAILLE PFGRNTAPAV AIAAMKLVAE
110 120 130 140 150
GRDELLLILP ADHVIEDQRA FQQALALATN AAEKGEMVLF GIPASRPETG
160 170 180 190 200
YGYIRASADA QLPEGVSRVQ SFVEKPDEAR AREFVAAGGY YWNSGMFLFR
210 220 230 240 250
ASRYLEELKK HDADIYDTCL LALERSQHDG DLVNIDAATF ECCPDNSIDY
260 270 280 290 300
AVMEKTSRAC VVPLSAGWND VGSWSSIWDV HAKDANGNVT KGDVLVHDSH
310 320 330 340 350
NCLVHGNGKL VSVIGLEDIV VVETKDAMMI AHKDRVQDVK HVVKDLDAQG
360 370 380 390 400
RSETQNHCEV YRPWGSYDSV DMGGRFQVKH ITVKPGARLS LQMHHHRAEH
410 420 430 440 450
WIVVSGTAQV TCDDKTFLLT ENQSTYIPIA SVHRLANPGK IPLEIIEVQS
460 470 480
GSYLGEDDIE RLEDVYGRTA EPALQVVAGS R
Length:481
Mass (Da):53,128
Last modified:January 10, 2001 - v3
Checksum:i2D497AF9059AEF97
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 272FL → LV in AAA25972 (PubMed:3089876).Curated
Sequence conflicti115 – 1151I → L in AAA25972 (PubMed:3089876).Curated
Sequence conflicti157 – 1571S → T in AAA25972 (PubMed:3089876).Curated
Sequence conflicti359 – 3602EV → DL in AAA25972 (PubMed:3089876).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14037 Genomic DNA. Translation: AAA25972.1.
AE004091 Genomic DNA. Translation: AAG06939.1.
PIRiA38598.
B83201.
RefSeqiNP_252241.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06939; AAG06939; PA3551.
GeneIDi879142.
KEGGipae:PA3551.
PATRICi19841717. VBIPseAer58763_3716.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14037 Genomic DNA. Translation: AAA25972.1.
AE004091 Genomic DNA. Translation: AAG06939.1.
PIRiA38598.
B83201.
RefSeqiNP_252241.1. NC_002516.2.

3D structure databases

ProteinModelPortaliP07874.
SMRiP07874. Positions 1-346, 362-453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3551.

Proteomic databases

PRIDEiP07874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06939; AAG06939; PA3551.
GeneIDi879142.
KEGGipae:PA3551.
PATRICi19841717. VBIPseAer58763_3716.

Organism-specific databases

PseudoCAPiPA3551.

Phylogenomic databases

eggNOGiCOG0662.
HOGENOMiHOG000153549.
InParanoidiP07874.
KOiK16011.
OMAiSDLGTWK.
OrthoDBiEOG6X3W6T.
PhylomeDBiP07874.

Enzyme and pathway databases

UniPathwayiUPA00126; UER00423.
UPA00126; UER00930.
BioCyciMetaCyc:MONOMER-13384.
BRENDAi2.7.7.13. 5087.
SABIO-RKP07874.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR006375. Man1P_GuaTrfase/Man6P_Isoase.
IPR001538. Man6P_isomerase-2_C.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF01050. MannoseP_isomer. 1 hit.
PF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01479. GMP_PMI. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis of the phosphomannose isomerase gene (pmi) of Pseudomonas aeruginosa and comparison with the corresponding Escherichia coli gene manA."
    Darzins A., Frantz B., Vanags R.I., Chakrabarty A.M.
    Gene 42:293-302(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. "Purification and characterization of phosphomannose isomerase-guanosine diphospho-D-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa."
    Shinabarger D., Berry A., May T.B., Rothmel R., Fialho A., Chakrabarty A.M.
    J. Biol. Chem. 266:2080-2088(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiALGA_PSEAE
AccessioniPrimary (citable) accession number: P07874
Secondary accession number(s): Q9HY66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1988
Last sequence update: January 10, 2001
Last modified: February 3, 2015
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.