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Protein

Peroxisomal acyl-coenzyme A oxidase 1

Gene

Acox1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 1 shows optimum activity with a chain length of 10 carbons while isoform 2 exhibits optimum activity with 14 carbons.1 Publication

Catalytic activityi

Acyl-CoA + O2 = trans-2,3-dehydroacyl-CoA + H2O2.

Cofactori

Pathwayi: peroxisomal fatty acid beta-oxidation

This protein is involved in the pathway peroxisomal fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway peroxisomal fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei139FAD1
Binding sitei178FAD; via amide nitrogen1
Active sitei421Proton acceptor2 Publications1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.3.3.6. 5301.
SABIO-RKP07872.
UniPathwayiUPA00661.

Chemistry databases

SwissLipidsiSLP:000000408.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal acyl-coenzyme A oxidase 1 (EC:1.3.3.6)
Short name:
AOX
Alternative name(s):
Palmitoyl-CoA oxidase
Cleaved into the following 3 chains:
Gene namesi
Name:Acox1
Synonyms:Acox
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619757. Acox1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4632.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000005521 – 661Peroxisomal acyl-CoA oxidase 1, A chainAdd BLAST661
ChainiPRO_00000005531 – 438Peroxisomal acyl-CoA oxidase 1, B chainAdd BLAST438
ChainiPRO_0000000554439 – 661Peroxisomal acyl-CoA oxidase 1, C chainAdd BLAST223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphoserineBy similarity1
Modified residuei65N6-acetyllysineBy similarity1
Modified residuei89N6-succinyllysineBy similarity1
Modified residuei90N6-succinyllysineBy similarity1
Modified residuei159N6-succinyllysineBy similarity1
Modified residuei216N6-acetyllysineBy similarity1
Modified residuei241N6-succinyllysineBy similarity1
Modified residuei255N6-acetyllysineBy similarity1
Modified residuei267N6-acetyllysineBy similarity1
Modified residuei272N6-acetyllysineBy similarity1
Modified residuei349N6-succinyllysineBy similarity1
Modified residuei437N6-acetyllysine; alternateBy similarity1
Modified residuei437N6-succinyllysine; alternateBy similarity1
Modified residuei446N6-acetyllysine; alternateBy similarity1
Modified residuei446N6-succinyllysine; alternateBy similarity1
Modified residuei500N6-acetyllysineBy similarity1
Modified residuei512N6-acetyllysine; alternateBy similarity1
Modified residuei512N6-succinyllysine; alternateBy similarity1
Modified residuei542N6-succinyllysineBy similarity1
Modified residuei637N6-acetyllysine; alternateBy similarity1
Modified residuei637N6-succinyllysine; alternateBy similarity1
Modified residuei643N6-succinyllysineBy similarity1
Modified residuei649PhosphoserineBy similarity1
Modified residuei652N6-acetyllysineBy similarity1
Modified residuei655N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP07872.
PRIDEiP07872.

PTM databases

iPTMnetiP07872.
PhosphoSitePlusiP07872.

Miscellaneous databases

PMAP-CutDBP07872.

Expressioni

Gene expression databases

BgeeiENSRNOG00000008755.

Interactioni

Subunit structurei

Homodimer. The enzyme contains three components A, B and C, the latter two being produced from the first by a proteolytic cleavage.2 Publications

Protein-protein interaction databases

IntActiP07872. 1 interactor.
MINTiMINT-1775779.
STRINGi10116.ENSRNOP00000051538.

Chemistry databases

BindingDBiP07872.

Structurei

Secondary structure

1661
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 11Combined sources9
Helixi16 – 24Combined sources9
Helixi27 – 41Combined sources15
Helixi44 – 46Combined sources3
Helixi51 – 53Combined sources3
Helixi56 – 76Combined sources21
Helixi82 – 93Combined sources12
Helixi101 – 105Combined sources5
Helixi107 – 111Combined sources5
Helixi117 – 128Combined sources12
Beta strandi134 – 137Combined sources4
Beta strandi143 – 145Combined sources3
Helixi147 – 149Combined sources3
Beta strandi153 – 157Combined sources5
Turni158 – 161Combined sources4
Beta strandi162 – 166Combined sources5
Turni170 – 172Combined sources3
Beta strandi173 – 175Combined sources3
Turni178 – 183Combined sources6
Beta strandi185 – 195Combined sources11
Beta strandi198 – 208Combined sources11
Turni212 – 214Combined sources3
Beta strandi221 – 225Combined sources5
Beta strandi229 – 231Combined sources3
Beta strandi238 – 248Combined sources11
Helixi249 – 251Combined sources3
Helixi279 – 306Combined sources28
Helixi321 – 323Combined sources3
Helixi325 – 353Combined sources29
Turni360 – 362Combined sources3
Helixi367 – 395Combined sources29
Helixi398 – 401Combined sources4
Helixi403 – 405Combined sources3
Helixi407 – 414Combined sources8
Helixi415 – 418Combined sources4
Beta strandi420 – 422Combined sources3
Helixi424 – 444Combined sources21
Helixi450 – 457Combined sources8
Helixi480 – 509Combined sources30
Helixi512 – 518Combined sources7
Helixi520 – 542Combined sources23
Helixi543 – 545Combined sources3
Helixi549 – 569Combined sources21
Helixi571 – 576Combined sources6
Helixi582 – 599Combined sources18
Helixi600 – 602Combined sources3
Helixi603 – 608Combined sources6
Helixi614 – 617Combined sources4
Helixi628 – 638Combined sources11
Helixi640 – 642Combined sources3
Beta strandi643 – 646Combined sources4
Helixi648 – 653Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IS2X-ray2.20A/B1-661[»]
2DDHX-ray2.07A1-661[»]
ProteinModelPortaliP07872.
SMRiP07872.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07872.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi659 – 661Microbody targeting signal3

Sequence similaritiesi

Belongs to the acyl-CoA oxidase family.Curated

Phylogenomic databases

eggNOGiKOG0136. Eukaryota.
COG1960. LUCA.
HOGENOMiHOG000181256.
HOVERGENiHBG050451.
InParanoidiP07872.
KOiK00232.
PhylomeDBiP07872.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR029320. Acyl-CoA_ox_N.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR012258. Acyl-CoA_oxidase.
IPR002655. Acyl-CoA_oxidase_C.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF01756. ACOX. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF14749. Acyl-CoA_ox_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000168. Acyl-CoA_oxidase. 1 hit.
SUPFAMiSSF47203. SSF47203. 2 hits.
SSF56645. SSF56645. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P07872-1) [UniParc]FASTAAdd to basket
Also known as: ACO-I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNPDLRKERA SATFNPELIT HILDGSPENT RRRREIENLI LNDPDFQHED
60 70 80 90 100
YNFLTRSQRY EVAVKKSATM VKKMREYGIS DPEEIMWFKK LYLANFVEPV
110 120 130 140 150
GLNYSMFIPT LLNQGTTAQQ EKWMRPSQEL QIIGTYAQTE MGHGTHLRGL
160 170 180 190 200
ETTATYDPKT QEFILNSPTV TSIKWWPGGL GKTSNHAIVL AQLITQGECY
210 220 230 240 250
GLHAFVVPIR EIGTHKPLPG ITVGDIGPKF GYEEMDNGYL KMDNYRIPRE
260 270 280 290 300
NMLMKYAQVK PDGTYVKPLS NKLTYGTMVF VRSFLVGNAA QSLSKACTIA
310 320 330 340 350
IRYSAVRRQS EIKQSEPEPQ ILDFQTQQYK LFPLLATAYA FHFVGRYMKE
360 370 380 390 400
TYLRINESIG QGDLSELPEL HALTAGLKAF TTWTANAGIE ECRMACGGHG
410 420 430 440 450
YSHSSGIPNI YVTFTPACTF EGENTVMMLQ TARFLMKIYD QVRSGKLVGG
460 470 480 490 500
MVSYLNDLPS QRIQPQQVAV WPTMVDINSL EGLTEAYKLR AARLVEIAAK
510 520 530 540 550
NLQTHVSHRK SKEVAWNLTS VDLVRASEAH CHYVVVKVFS DKLPKIQDKA
560 570 580 590 600
VQAVLRNLCL LYSLYGISQK GGDFLEGSII TGAQLSQVNA RILELLTLIR
610 620 630 640 650
PNAVALVDAF DFKDMTLGSV LGRYDGNVYE NLFEWAKKSP LNKTEVHESY
660
HKHLKPLQSK L
Length:661
Mass (Da):74,679
Last modified:August 1, 1988 - v1
Checksum:i24B1F10DF066C29E
GO
Isoform 2 (identifier: P07872-2) [UniParc]FASTAAdd to basket
Also known as: ACO-II

The sequence of this isoform differs from the canonical sequence as follows:
     90-133: KLYLANFVEP...MRPSQELQII → NSVHRGHPEP...FMPAWNLEIT

Show »
Length:661
Mass (Da):74,691
Checksum:i23EBDE0CDEE70559
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00014790 – 133KLYLA…ELQII → NSVHRGHPEPLDLHLGMFLP TLLHQATAEQQERFFMPAWN LEIT in isoform 2. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02752 mRNA. Translation: AAA40666.1.
BC085743 mRNA. Translation: AAH85743.1.
J02753 Genomic DNA. Translation: AAA40667.1.
PIRiA29328. OXRTA1.
B29328. OXRTA2.
RefSeqiNP_059036.1. NM_017340.2. [P07872-1]
UniGeneiRn.31796.

Genome annotation databases

GeneIDi50681.
KEGGirno:50681.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02752 mRNA. Translation: AAA40666.1.
BC085743 mRNA. Translation: AAH85743.1.
J02753 Genomic DNA. Translation: AAA40667.1.
PIRiA29328. OXRTA1.
B29328. OXRTA2.
RefSeqiNP_059036.1. NM_017340.2. [P07872-1]
UniGeneiRn.31796.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IS2X-ray2.20A/B1-661[»]
2DDHX-ray2.07A1-661[»]
ProteinModelPortaliP07872.
SMRiP07872.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP07872. 1 interactor.
MINTiMINT-1775779.
STRINGi10116.ENSRNOP00000051538.

Chemistry databases

BindingDBiP07872.
ChEMBLiCHEMBL4632.
SwissLipidsiSLP:000000408.

PTM databases

iPTMnetiP07872.
PhosphoSitePlusiP07872.

Proteomic databases

PaxDbiP07872.
PRIDEiP07872.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi50681.
KEGGirno:50681.

Organism-specific databases

CTDi51.
RGDi619757. Acox1.

Phylogenomic databases

eggNOGiKOG0136. Eukaryota.
COG1960. LUCA.
HOGENOMiHOG000181256.
HOVERGENiHBG050451.
InParanoidiP07872.
KOiK00232.
PhylomeDBiP07872.

Enzyme and pathway databases

UniPathwayiUPA00661.
BRENDAi1.3.3.6. 5301.
SABIO-RKP07872.

Miscellaneous databases

EvolutionaryTraceiP07872.
PMAP-CutDBP07872.
PROiP07872.

Gene expression databases

BgeeiENSRNOG00000008755.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR029320. Acyl-CoA_ox_N.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR012258. Acyl-CoA_oxidase.
IPR002655. Acyl-CoA_oxidase_C.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF01756. ACOX. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF14749. Acyl-CoA_ox_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000168. Acyl-CoA_oxidase. 1 hit.
SUPFAMiSSF47203. SSF47203. 2 hits.
SSF56645. SSF56645. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiACOX1_RAT
AccessioniPrimary (citable) accession number: P07872
Secondary accession number(s): P11354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 2, 2016
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.