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P07866

- LTE1_YEAST

UniProt

P07866 - LTE1_YEAST

Protein

Guanine nucleotide exchange factor LTE1

Gene

LTE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    GDP-GTP exchange factor for TEM1, a Ras-like protein, component of the mitotic exit network (MEN). Activation of TEM1 by LTE1 in the bud ultimately leads to activation of CDC15 followed by the release of CDC14 from the nucleolus, which then inactivates cyclin-dependent kinases (CDKs) activity by several mechanism. Required for TEM1 localization to the bud cortex during mitotic exit. Fine-tunes the timing of the mitotic exit and couples this event with cytokinesis.
    Involved in proprotein-processing like proalpha factor-processing in the secretory pathway.

    GO - Molecular functioni

    1. guanyl-nucleotide exchange factor activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. mitotic spindle orientation checkpoint Source: SGD
    3. positive regulation of GTPase activity Source: GOC
    4. regulation of exit from mitosis Source: SGD
    5. regulation of small GTPase mediated signal transduction Source: InterPro
    6. small GTPase mediated signal transduction Source: InterPro
    7. vesicle-mediated transport Source: SGD

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28835-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide exchange factor LTE1
    Alternative name(s):
    Low temperature essential protein 1
    Gene namesi
    Name:LTE1
    Synonyms:EIS4, MSI2
    Ordered Locus Names:YAL024C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL024c.
    SGDiS000000022. LTE1.

    Subcellular locationi

    Cytoplasm. Bud
    Note: The localization to the bud requires interaction with KEL1, as well as the presence of septins, CDC42, CLA4 and RAS2.

    GO - Cellular componenti

    1. cellular bud Source: SGD
    2. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14351435Guanine nucleotide exchange factor LTE1PRO_0000068884Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei271 – 2711Phosphoserine1 Publication
    Modified residuei559 – 5591Phosphoserine1 Publication
    Modified residuei689 – 6891Phosphoserine1 Publication
    Modified residuei691 – 6911Phosphothreonine1 Publication
    Modified residuei808 – 8081Phosphoserine1 Publication
    Modified residuei810 – 8101Phosphoserine1 Publication
    Modified residuei1028 – 10281Phosphoserine2 Publications
    Modified residuei1109 – 11091Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated by CDC28 in a cell cycle-dependent manner and in response to nocodazole. Dephosphorylion by CDC14 triggers LTE1 release from bud cortex during the exit of mitosis.6 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP07866.
    PaxDbiP07866.

    Expressioni

    Gene expression databases

    GenevestigatoriP07866.

    Interactioni

    Subunit structurei

    Interacts with CDC24, CDC42, KEL1, KEL2, RAS2 and TEM1.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    KEL1P388534EBI-10243,EBI-9619
    RAS2P011205EBI-10243,EBI-14838

    Protein-protein interaction databases

    BioGridi31742. 391 interactions.
    DIPiDIP-337N.
    IntActiP07866. 56 interactions.
    MINTiMINT-620988.
    STRINGi4932.YAL024C.

    Structurei

    3D structure databases

    ProteinModelPortaliP07866.
    SMRiP07866. Positions 1195-1434.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 157133N-terminal Ras-GEFPROSITE-ProRule annotationAdd
    BLAST
    Domaini1194 – 1434241Ras-GEFPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the LTE1 family.Curated
    Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
    Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG330349.
    HOGENOMiHOG000066134.
    KOiK06681.
    OMAiFILMYDS.
    OrthoDBiEOG75QRC8.

    Family and domain databases

    Gene3Di1.10.840.10. 1 hit.
    InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
    IPR019804. Ras_G-nucl-exch_fac_CS.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    [Graphical view]
    PfamiPF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    [Graphical view]
    SMARTiSM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48366. SSF48366. 2 hits.
    PROSITEiPS00720. RASGEF. 1 hit.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07866-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEIFSQKDYY PTPSSNVISY ESDCVSKPVN SADLPALIVH LSSPLEGVDY     50
    NASADFFLIY RNFITPQDLH DLLIYRFRWC IREITTNAAK AKRRRIGEVA 100
    LVRTFVLLRH SILNYFVQDF LPNITLRLRL IEFLNDKHIE QYPKIISSCI 150
    INLKKNWVHC SKLVWENIEL NEPDKLDFDA WLHYSLKDFT QLESLHKRGS 200
    RLSIYARQSF ASPDFRNQSV LSLYKTSDVF RLPEKLQSSN SSKNQRSPSM 250
    LLFPDNTSNV YSKHRIAKEP SVDNESEDMS DSKQKISHLS KVTLVSTLMK 300
    GVDYPSSYAV DKIMPPTPAK KVEFILNSLY IPEDLNEQSG TLQGTSTTSS 350
    LDNNSNSNSR SNTSSMSVLH RSAIGLLAKW MKNHNRHDSS NDKKFMSAIK 400
    PANQKPEMDA FVKYVVSISS LNRKSSKEEE EEFLNSDSSK FDILSARTID 450
    EVESLLHLQN QLIEKVQTHS NNNRGPTVNV DCERREHIHD IKILQQNSFK 500
    PSNDNFSAMD NLDLYQTVSS IAQSVISLTN TLNKQLQNNE SNMQPSPSYD 550
    ALQRRKVKSL TTAYYNKMHG SYSAESMRLF DKDNSSSRTD ENGPQRLLFH 600
    ETDKTNSEAI TNMTPRRKNH SQSQKSMTSS PLKNVLPDLK ESSPLNDSRE 650
    DTESITYSYD SELSSSSPPR DTVTKKSRKV RNIVNNTDSP TLKTKTGFLN 700
    LREFTFEDTK SLDEKKSTID GLEKNYDNKE NQESEYESTK KLDNSLDASS 750
    EANNYDITTR KKHSSCNHKI KQAVVRPASG RISISRVQSI AITPTKELSI 800
    VDPEQNKSNS VIEEISEIEP LNLEYNKKSA LYSDTSSTVI SISTSKLFES 850
    AQNSPLKQTQ NPQREFPNGT SVSETNRIRL SIAPTIESVV SDLNSITTGS 900
    TVETFETSRD LPVPHQRIIN LREEYQRGNQ DIISNTSSLH ELKTIDLSDS 950
    NNDLESPSTH AKNNKYFFSP DDGSIDVASP MKNVEELKSK FLKNESETNS 1000
    NISGSVLTMD DIDINDTSSA RNTRRANSES AFTGSLNKKN LNEIANMLDD 1050
    SINDDPITVA LMKLEGTYEK IPEKPENTKS SDAIGIKTSK LADEVEMLNL 1100
    NNLPSFQNSP AEKRKSLLIE RRRQTIMNIP FTPDQSEKEG FTSSSPEKID 1150
    VSANVDVAVQ AAQIQELIGQ YRIHDSRLMI SNNESHVPFI LMYDSLSVAQ 1200
    QMTLIEKEIL GEIDWKDLLD LKMKHEGPQV ISWLQLLVRN ETLSGIDLAI 1250
    SRFNLTVDWI ISEILLTKSS KMKRNVIQRF IHVADHCRTF QNFNTLMEII 1300
    LALSSSVVQK FTDAWRLIEP GDLLTWEELK KIPSLDRNYS TIRNLLNSVN 1350
    PLVGCVPFIV VYLSDLSANA EKKDWILEDK VVNYNKFDTN VQIVKNFIQR 1400
    VQWSKFYTFK VNHELLSKCV YISTLTQEEI NELST 1435
    Length:1,435
    Mass (Da):163,150
    Last modified:October 1, 1994 - v2
    Checksum:iEED7E5150BECA3DE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti998 – 100912TNSNI…SVLTM → LIVHIRKCIDN in BAA04820. (PubMed:7941731)CuratedAdd
    BLAST
    Sequence conflicti1161 – 11633AAQ → GE in AAA34746. (PubMed:3332963)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20125 Genomic DNA. Translation: AAA50468.1.
    U12980 Genomic DNA. Translation: AAC05008.1.
    D21354 Genomic DNA. Translation: BAA04820.1.
    M16076 Genomic DNA. Translation: AAA34746.1.
    J03852 Genomic DNA. Translation: AAA34751.1.
    BK006935 Genomic DNA. Translation: DAA06964.1.
    PIRiS51997. BVBYL1.
    RefSeqiNP_009378.1. NM_001178169.1.

    Genome annotation databases

    EnsemblFungiiYAL024C; YAL024C; YAL024C.
    GeneIDi851209.
    KEGGisce:YAL024C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20125 Genomic DNA. Translation: AAA50468.1 .
    U12980 Genomic DNA. Translation: AAC05008.1 .
    D21354 Genomic DNA. Translation: BAA04820.1 .
    M16076 Genomic DNA. Translation: AAA34746.1 .
    J03852 Genomic DNA. Translation: AAA34751.1 .
    BK006935 Genomic DNA. Translation: DAA06964.1 .
    PIRi S51997. BVBYL1.
    RefSeqi NP_009378.1. NM_001178169.1.

    3D structure databases

    ProteinModelPortali P07866.
    SMRi P07866. Positions 1195-1434.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31742. 391 interactions.
    DIPi DIP-337N.
    IntActi P07866. 56 interactions.
    MINTi MINT-620988.
    STRINGi 4932.YAL024C.

    Proteomic databases

    MaxQBi P07866.
    PaxDbi P07866.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL024C ; YAL024C ; YAL024C .
    GeneIDi 851209.
    KEGGi sce:YAL024C.

    Organism-specific databases

    CYGDi YAL024c.
    SGDi S000000022. LTE1.

    Phylogenomic databases

    eggNOGi NOG330349.
    HOGENOMi HOG000066134.
    KOi K06681.
    OMAi FILMYDS.
    OrthoDBi EOG75QRC8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28835-MONOMER.

    Miscellaneous databases

    NextBioi 968082.

    Gene expression databases

    Genevestigatori P07866.

    Family and domain databases

    Gene3Di 1.10.840.10. 1 hit.
    InterProi IPR000651. Ras-like_Gua-exchang_fac_N.
    IPR019804. Ras_G-nucl-exch_fac_CS.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    [Graphical view ]
    Pfami PF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    [Graphical view ]
    SMARTi SM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48366. SSF48366. 2 hits.
    PROSITEi PS00720. RASGEF. 1 hit.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "LTE1 of Saccharomyces cerevisiae is a 1435 codon open reading frame that has sequence similarities to guanine nucleotide releasing factors."
      Keng T., Clark M.W., Storms R.K., Fortin N., Zhong W., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
      Yeast 10:953-958(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Isolation of a CDC25 family gene, MSI2/LTE1, as a multicopy suppressor of ira1."
      Shirayama M., Matsui Y., Tanaka K., Toh-e A.
      Yeast 10:451-461(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    5. "Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation of the MAK16 gene and analysis of an adjacent gene essential for growth at low temperatures."
      Wickner R.B., Koh T.J., Crowley J.C., O'Neil J., Kaback D.B.
      Yeast 3:51-57(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1127-1435, FUNCTION.
    6. "Host function of MAK16: G1 arrest by a mak16 mutant of Saccharomyces cerevisiae."
      Wickner R.B.
      Proc. Natl. Acad. Sci. U.S.A. 85:6007-6011(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1404-1435.
    7. "The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase."
      Shirayama M., Matsui Y., Toh-e A.
      Mol. Cell. Biol. 14:7476-7482(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex."
      Shou W., Seol J.H., Shevchenko A., Baskerville C., Moazed D., Chen Z.W.S., Jang J., Shevchenko A., Charbonneau H., Deshaies R.J.
      Cell 97:233-244(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "A mechanism for coupling exit from mitosis to partitioning of the nucleus."
      Bardin A.J., Visintin R., Amon A.
      Cell 102:21-31(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    10. "Nud1p links astral microtubule organization and the control of exit from mitosis."
      Gruneberg U., Campbell K., Simpson C., Grindlay J., Schiebel E.
      EMBO J. 19:6475-6488(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "The surveillance mechanism of the spindle position checkpoint in yeast."
      Adames N.R., Oberle J.R., Cooper J.A.
      J. Cell Biol. 153:159-168(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "The Bub2-dependent mitotic pathway in yeast acts every cell cycle and regulates cytokinesis."
      Lee S.E., Jensen S., Frenz L.M., Johnson A.L., Fesquet D., Johnston L.H.
      J. Cell Sci. 114:2345-2354(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION.
    13. "Control of Lte1 localization by cell polarity determinants and Cdc14."
      Seshan A., Bardin A.J., Amon A.
      Curr. Biol. 12:2098-2110(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH KEL1.
    14. "A role for cell polarity proteins in mitotic exit."
      Hoefken T., Schiebel E.
      EMBO J. 21:4851-4862(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH CDC24; CDC42; KEL1; KEL2 AND TEM1.
    15. "Spatial regulation of the guanine nucleotide exchange factor Lte1 in Saccharomyces cerevisiae."
      Jensen S., Geymonat M., Johnson A.L., Segal M., Johnston L.H.
      J. Cell Sci. 115:4977-4991(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION BY CDC28, DEPHOSPHORYLATION BY CDC14.
    16. Cited for: FUNCTION, SUBCELLULAR LOCATION.
    17. "Ras recruits mitotic exit regulator Lte1 to the bud cortex in budding yeast."
      Yoshida S., Ichihashi R., Toh-e A.
      J. Cell Biol. 161:889-897(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RAS2.
    18. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    19. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    20. "Temporal coupling of spindle disassembly and cytokinesis is disrupted by deletion of LTE1 in budding yeast."
      Jensen S., Johnson A.L., Johnston L.H., Segal M.
      Cell Cycle 3:817-822(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    21. "The differential roles of budding yeast Tem1p, Cdc15p, and Bub2p protein dynamics in mitotic exit."
      Molk J.N., Schuyler S.C., Liu J.Y., Evans J.G., Salmon E.D., Pellman D., Bloom K.
      Mol. Biol. Cell 15:1519-1532(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    22. "A role for Lte1p (a low temperature essential protein involved in mitosis) in proprotein processing in the yeast secretory pathway."
      Zhao X., Chang A.Y., Toh-e A., Arvan P.
      J. Biol. Chem. 282:1670-1678(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    23. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    24. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-271 AND SER-1028, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    25. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-559; SER-689; THR-691; SER-808; SER-810; SER-1028 AND SER-1109, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLTE1_YEAST
    AccessioniPrimary (citable) accession number: P07866
    Secondary accession number(s): D6VPJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 143 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 304 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3