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P07866

- LTE1_YEAST

UniProt

P07866 - LTE1_YEAST

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Protein

Guanine nucleotide exchange factor LTE1

Gene

LTE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GDP-GTP exchange factor for TEM1, a Ras-like protein, component of the mitotic exit network (MEN). Activation of TEM1 by LTE1 in the bud ultimately leads to activation of CDC15 followed by the release of CDC14 from the nucleolus, which then inactivates cyclin-dependent kinases (CDKs) activity by several mechanism. Required for TEM1 localization to the bud cortex during mitotic exit. Fine-tunes the timing of the mitotic exit and couples this event with cytokinesis.
Involved in proprotein-processing like proalpha factor-processing in the secretory pathway.

GO - Molecular functioni

  1. guanyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. mitotic spindle orientation checkpoint Source: SGD
  3. positive regulation of GTPase activity Source: GOC
  4. regulation of exit from mitosis Source: SGD
  5. regulation of small GTPase mediated signal transduction Source: InterPro
  6. small GTPase mediated signal transduction Source: InterPro
  7. vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-28835-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor LTE1
Alternative name(s):
Low temperature essential protein 1
Gene namesi
Name:LTE1
Synonyms:EIS4, MSI2
Ordered Locus Names:YAL024C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL024c.
SGDiS000000022. LTE1.

Subcellular locationi

Cytoplasm. Bud
Note: The localization to the bud requires interaction with KEL1, as well as the presence of septins, CDC42, CLA4 and RAS2.

GO - Cellular componenti

  1. cellular bud Source: SGD
  2. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14351435Guanine nucleotide exchange factor LTE1PRO_0000068884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei271 – 2711Phosphoserine1 Publication
Modified residuei559 – 5591Phosphoserine1 Publication
Modified residuei689 – 6891Phosphoserine1 Publication
Modified residuei691 – 6911Phosphothreonine1 Publication
Modified residuei808 – 8081Phosphoserine1 Publication
Modified residuei810 – 8101Phosphoserine1 Publication
Modified residuei1028 – 10281Phosphoserine2 Publications
Modified residuei1109 – 11091Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by CDC28 in a cell cycle-dependent manner and in response to nocodazole. Dephosphorylion by CDC14 triggers LTE1 release from bud cortex during the exit of mitosis.6 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07866.
PaxDbiP07866.

Expressioni

Gene expression databases

GenevestigatoriP07866.

Interactioni

Subunit structurei

Interacts with CDC24, CDC42, KEL1, KEL2, RAS2 and TEM1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KEL1P388534EBI-10243,EBI-9619
RAS2P011205EBI-10243,EBI-14838

Protein-protein interaction databases

BioGridi31742. 391 interactions.
DIPiDIP-337N.
IntActiP07866. 56 interactions.
MINTiMINT-620988.
STRINGi4932.YAL024C.

Structurei

3D structure databases

ProteinModelPortaliP07866.
SMRiP07866. Positions 1195-1434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 157133N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini1194 – 1434241Ras-GEFPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the LTE1 family.Curated
Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG330349.
HOGENOMiHOG000066134.
InParanoidiP07866.
KOiK06681.
OMAiFILMYDS.
OrthoDBiEOG75QRC8.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
[Graphical view]
PfamiPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 2 hits.
PROSITEiPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07866-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEIFSQKDYY PTPSSNVISY ESDCVSKPVN SADLPALIVH LSSPLEGVDY
60 70 80 90 100
NASADFFLIY RNFITPQDLH DLLIYRFRWC IREITTNAAK AKRRRIGEVA
110 120 130 140 150
LVRTFVLLRH SILNYFVQDF LPNITLRLRL IEFLNDKHIE QYPKIISSCI
160 170 180 190 200
INLKKNWVHC SKLVWENIEL NEPDKLDFDA WLHYSLKDFT QLESLHKRGS
210 220 230 240 250
RLSIYARQSF ASPDFRNQSV LSLYKTSDVF RLPEKLQSSN SSKNQRSPSM
260 270 280 290 300
LLFPDNTSNV YSKHRIAKEP SVDNESEDMS DSKQKISHLS KVTLVSTLMK
310 320 330 340 350
GVDYPSSYAV DKIMPPTPAK KVEFILNSLY IPEDLNEQSG TLQGTSTTSS
360 370 380 390 400
LDNNSNSNSR SNTSSMSVLH RSAIGLLAKW MKNHNRHDSS NDKKFMSAIK
410 420 430 440 450
PANQKPEMDA FVKYVVSISS LNRKSSKEEE EEFLNSDSSK FDILSARTID
460 470 480 490 500
EVESLLHLQN QLIEKVQTHS NNNRGPTVNV DCERREHIHD IKILQQNSFK
510 520 530 540 550
PSNDNFSAMD NLDLYQTVSS IAQSVISLTN TLNKQLQNNE SNMQPSPSYD
560 570 580 590 600
ALQRRKVKSL TTAYYNKMHG SYSAESMRLF DKDNSSSRTD ENGPQRLLFH
610 620 630 640 650
ETDKTNSEAI TNMTPRRKNH SQSQKSMTSS PLKNVLPDLK ESSPLNDSRE
660 670 680 690 700
DTESITYSYD SELSSSSPPR DTVTKKSRKV RNIVNNTDSP TLKTKTGFLN
710 720 730 740 750
LREFTFEDTK SLDEKKSTID GLEKNYDNKE NQESEYESTK KLDNSLDASS
760 770 780 790 800
EANNYDITTR KKHSSCNHKI KQAVVRPASG RISISRVQSI AITPTKELSI
810 820 830 840 850
VDPEQNKSNS VIEEISEIEP LNLEYNKKSA LYSDTSSTVI SISTSKLFES
860 870 880 890 900
AQNSPLKQTQ NPQREFPNGT SVSETNRIRL SIAPTIESVV SDLNSITTGS
910 920 930 940 950
TVETFETSRD LPVPHQRIIN LREEYQRGNQ DIISNTSSLH ELKTIDLSDS
960 970 980 990 1000
NNDLESPSTH AKNNKYFFSP DDGSIDVASP MKNVEELKSK FLKNESETNS
1010 1020 1030 1040 1050
NISGSVLTMD DIDINDTSSA RNTRRANSES AFTGSLNKKN LNEIANMLDD
1060 1070 1080 1090 1100
SINDDPITVA LMKLEGTYEK IPEKPENTKS SDAIGIKTSK LADEVEMLNL
1110 1120 1130 1140 1150
NNLPSFQNSP AEKRKSLLIE RRRQTIMNIP FTPDQSEKEG FTSSSPEKID
1160 1170 1180 1190 1200
VSANVDVAVQ AAQIQELIGQ YRIHDSRLMI SNNESHVPFI LMYDSLSVAQ
1210 1220 1230 1240 1250
QMTLIEKEIL GEIDWKDLLD LKMKHEGPQV ISWLQLLVRN ETLSGIDLAI
1260 1270 1280 1290 1300
SRFNLTVDWI ISEILLTKSS KMKRNVIQRF IHVADHCRTF QNFNTLMEII
1310 1320 1330 1340 1350
LALSSSVVQK FTDAWRLIEP GDLLTWEELK KIPSLDRNYS TIRNLLNSVN
1360 1370 1380 1390 1400
PLVGCVPFIV VYLSDLSANA EKKDWILEDK VVNYNKFDTN VQIVKNFIQR
1410 1420 1430
VQWSKFYTFK VNHELLSKCV YISTLTQEEI NELST
Length:1,435
Mass (Da):163,150
Last modified:October 1, 1994 - v2
Checksum:iEED7E5150BECA3DE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti998 – 100912TNSNI…SVLTM → LIVHIRKCIDN in BAA04820. (PubMed:7941731)CuratedAdd
BLAST
Sequence conflicti1161 – 11633AAQ → GE in AAA34746. (PubMed:3332963)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20125 Genomic DNA. Translation: AAA50468.1.
U12980 Genomic DNA. Translation: AAC05008.1.
D21354 Genomic DNA. Translation: BAA04820.1.
M16076 Genomic DNA. Translation: AAA34746.1.
J03852 Genomic DNA. Translation: AAA34751.1.
BK006935 Genomic DNA. Translation: DAA06964.1.
PIRiS51997. BVBYL1.
RefSeqiNP_009378.1. NM_001178169.1.

Genome annotation databases

EnsemblFungiiYAL024C; YAL024C; YAL024C.
GeneIDi851209.
KEGGisce:YAL024C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20125 Genomic DNA. Translation: AAA50468.1 .
U12980 Genomic DNA. Translation: AAC05008.1 .
D21354 Genomic DNA. Translation: BAA04820.1 .
M16076 Genomic DNA. Translation: AAA34746.1 .
J03852 Genomic DNA. Translation: AAA34751.1 .
BK006935 Genomic DNA. Translation: DAA06964.1 .
PIRi S51997. BVBYL1.
RefSeqi NP_009378.1. NM_001178169.1.

3D structure databases

ProteinModelPortali P07866.
SMRi P07866. Positions 1195-1434.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31742. 391 interactions.
DIPi DIP-337N.
IntActi P07866. 56 interactions.
MINTi MINT-620988.
STRINGi 4932.YAL024C.

Proteomic databases

MaxQBi P07866.
PaxDbi P07866.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL024C ; YAL024C ; YAL024C .
GeneIDi 851209.
KEGGi sce:YAL024C.

Organism-specific databases

CYGDi YAL024c.
SGDi S000000022. LTE1.

Phylogenomic databases

eggNOGi NOG330349.
HOGENOMi HOG000066134.
InParanoidi P07866.
KOi K06681.
OMAi FILMYDS.
OrthoDBi EOG75QRC8.

Enzyme and pathway databases

BioCyci YEAST:G3O-28835-MONOMER.

Miscellaneous databases

NextBioi 968082.

Gene expression databases

Genevestigatori P07866.

Family and domain databases

Gene3Di 1.10.840.10. 1 hit.
InterProi IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
[Graphical view ]
Pfami PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view ]
SMARTi SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view ]
SUPFAMi SSF48366. SSF48366. 2 hits.
PROSITEi PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "LTE1 of Saccharomyces cerevisiae is a 1435 codon open reading frame that has sequence similarities to guanine nucleotide releasing factors."
    Keng T., Clark M.W., Storms R.K., Fortin N., Zhong W., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
    Yeast 10:953-958(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Isolation of a CDC25 family gene, MSI2/LTE1, as a multicopy suppressor of ira1."
    Shirayama M., Matsui Y., Tanaka K., Toh-e A.
    Yeast 10:451-461(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  5. "Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation of the MAK16 gene and analysis of an adjacent gene essential for growth at low temperatures."
    Wickner R.B., Koh T.J., Crowley J.C., O'Neil J., Kaback D.B.
    Yeast 3:51-57(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1127-1435, FUNCTION.
  6. "Host function of MAK16: G1 arrest by a mak16 mutant of Saccharomyces cerevisiae."
    Wickner R.B.
    Proc. Natl. Acad. Sci. U.S.A. 85:6007-6011(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1404-1435.
  7. "The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase."
    Shirayama M., Matsui Y., Toh-e A.
    Mol. Cell. Biol. 14:7476-7482(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex."
    Shou W., Seol J.H., Shevchenko A., Baskerville C., Moazed D., Chen Z.W.S., Jang J., Shevchenko A., Charbonneau H., Deshaies R.J.
    Cell 97:233-244(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "A mechanism for coupling exit from mitosis to partitioning of the nucleus."
    Bardin A.J., Visintin R., Amon A.
    Cell 102:21-31(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. "Nud1p links astral microtubule organization and the control of exit from mitosis."
    Gruneberg U., Campbell K., Simpson C., Grindlay J., Schiebel E.
    EMBO J. 19:6475-6488(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "The surveillance mechanism of the spindle position checkpoint in yeast."
    Adames N.R., Oberle J.R., Cooper J.A.
    J. Cell Biol. 153:159-168(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "The Bub2-dependent mitotic pathway in yeast acts every cell cycle and regulates cytokinesis."
    Lee S.E., Jensen S., Frenz L.M., Johnson A.L., Fesquet D., Johnston L.H.
    J. Cell Sci. 114:2345-2354(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  13. "Control of Lte1 localization by cell polarity determinants and Cdc14."
    Seshan A., Bardin A.J., Amon A.
    Curr. Biol. 12:2098-2110(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH KEL1.
  14. "A role for cell polarity proteins in mitotic exit."
    Hoefken T., Schiebel E.
    EMBO J. 21:4851-4862(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH CDC24; CDC42; KEL1; KEL2 AND TEM1.
  15. "Spatial regulation of the guanine nucleotide exchange factor Lte1 in Saccharomyces cerevisiae."
    Jensen S., Geymonat M., Johnson A.L., Segal M., Johnston L.H.
    J. Cell Sci. 115:4977-4991(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION BY CDC28, DEPHOSPHORYLATION BY CDC14.
  16. Cited for: FUNCTION, SUBCELLULAR LOCATION.
  17. "Ras recruits mitotic exit regulator Lte1 to the bud cortex in budding yeast."
    Yoshida S., Ichihashi R., Toh-e A.
    J. Cell Biol. 161:889-897(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RAS2.
  18. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  19. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  20. "Temporal coupling of spindle disassembly and cytokinesis is disrupted by deletion of LTE1 in budding yeast."
    Jensen S., Johnson A.L., Johnston L.H., Segal M.
    Cell Cycle 3:817-822(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  21. "The differential roles of budding yeast Tem1p, Cdc15p, and Bub2p protein dynamics in mitotic exit."
    Molk J.N., Schuyler S.C., Liu J.Y., Evans J.G., Salmon E.D., Pellman D., Bloom K.
    Mol. Biol. Cell 15:1519-1532(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  22. "A role for Lte1p (a low temperature essential protein involved in mitosis) in proprotein processing in the yeast secretory pathway."
    Zhao X., Chang A.Y., Toh-e A., Arvan P.
    J. Biol. Chem. 282:1670-1678(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  23. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  24. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-271 AND SER-1028, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  25. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-559; SER-689; THR-691; SER-808; SER-810; SER-1028 AND SER-1109, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLTE1_YEAST
AccessioniPrimary (citable) accession number: P07866
Secondary accession number(s): D6VPJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 304 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3