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Protein

L-lactate dehydrogenase C chain

Gene

LDHC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possible role in sperm motility.

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathway:ipyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase A-like 6A (LDHAL6A), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase, L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase A-like 6B (LDHAL6B), L-lactate dehydrogenase (HEL-S-133P), L-lactate dehydrogenase (LDHAL6A), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHAL6A), L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase A chain (LDHA), L-lactate dehydrogenase B chain (LDHB), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase, L-lactate dehydrogenase C chain (LDHC)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991NADBy similarity
Binding sitei106 – 1061SubstrateBy similarity
Binding sitei138 – 1381NAD or substrateBy similarity
Binding sitei169 – 1691SubstrateBy similarity
Active sitei193 – 1931Proton acceptorBy similarity
Binding sitei248 – 2481SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 5729NADBy similarityAdd
BLAST

GO - Molecular functioni

  • L-lactate dehydrogenase activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase C chain (EC:1.1.1.27)
Short name:
LDH-C
Alternative name(s):
Cancer/testis antigen 32
Short name:
CT32
LDH testis subunit
LDH-X
Gene namesi
Name:LDHC
Synonyms:LDH3, LDHX
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:6544. LDHC.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • motile cilium Source: Ensembl
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30328.

Polymorphism and mutation databases

BioMutaiLDHC.
DMDMi76363520.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 332331L-lactate dehydrogenase C chainPRO_0000168479Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei239 – 2391PhosphotyrosineBy similarity
Modified residuei301 – 3011Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07864.
PaxDbiP07864.
PRIDEiP07864.

PTM databases

PhosphoSiteiP07864.

Expressioni

Gene expression databases

BgeeiP07864.
CleanExiHS_LDHC.
ExpressionAtlasiP07864. baseline and differential.
GenevisibleiP07864. HS.

Organism-specific databases

HPAiHPA045442.

Interactioni

Subunit structurei

Homotetramer. Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi110140. 7 interactions.
STRINGi9606.ENSP00000280704.

Structurei

3D structure databases

ProteinModelPortaliP07864.
SMRiP07864. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiCOG0039.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP07864.
KOiK00016.
OMAiKNSQCKI.
OrthoDBiEOG7X0VH3.
PhylomeDBiP07864.
TreeFamiTF314963.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVKEQLIE KLIEDDENSQ CKITIVGTGA VGMACAISIL LKDLADELAL
60 70 80 90 100
VDVALDKLKG EMMDLQHGSL FFSTSKITSG KDYSVSANSR IVIVTAGARQ
110 120 130 140 150
QEGETRLALV QRNVAIMKSI IPAIVHYSPD CKILVVSNPV DILTYIVWKI
160 170 180 190 200
SGLPVTRVIG SGCNLDSARF RYLIGEKLGV HPTSCHGWII GEHGDSSVPL
210 220 230 240 250
WSGVNVAGVA LKTLDPKLGT DSDKEHWKNI HKQVIQSAYE IIKLKGYTSW
260 270 280 290 300
AIGLSVMDLV GSILKNLRRV HPVSTMVKGL YGIKEELFLS IPCVLGRNGV
310 320 330
SDVVKINLNS EEEALFKKSA ETLWNIQKDL IF
Length:332
Mass (Da):36,311
Last modified:January 23, 2007 - v4
Checksum:iDF7047FF5BCB101B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771I → V in AAA59507 (PubMed:2440048).Curated
Sequence conflicti77 – 771I → V in AAA59508 (PubMed:2930531).Curated
Sequence conflicti82 – 821D → G in AAP37402 (PubMed:14985855).Curated
Sequence conflicti119 – 1191S → I in AAA59507 (PubMed:2440048).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti285 – 2851E → Q.
Corresponds to variant rs2230150 [ dbSNP | Ensembl ].
VAR_034068

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02938 mRNA. Translation: AAA59507.1.
AH002865 Genomic DNA. Translation: AAA59508.1.
U13680 mRNA. Translation: AAA21348.1.
AY286300 mRNA. Translation: AAP37402.1.
CH471064 Genomic DNA. Translation: EAW68392.1.
CH471064 Genomic DNA. Translation: EAW68394.1.
BC019249 mRNA. Translation: AAH19249.3.
BC064388 mRNA. Translation: AAH64388.1.
BC090043 mRNA. Translation: AAH90043.1.
CCDSiCCDS7840.1.
PIRiA30933. DEHULC.
RefSeqiNP_002292.1. NM_002301.4.
NP_059144.1. NM_017448.3.
UniGeneiHs.654377.

Genome annotation databases

EnsembliENST00000280704; ENSP00000280704; ENSG00000166796.
ENST00000541669; ENSP00000437783; ENSG00000166796.
GeneIDi3948.
KEGGihsa:3948.
UCSCiuc001mom.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Lactate dehydrogenase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02938 mRNA. Translation: AAA59507.1.
AH002865 Genomic DNA. Translation: AAA59508.1.
U13680 mRNA. Translation: AAA21348.1.
AY286300 mRNA. Translation: AAP37402.1.
CH471064 Genomic DNA. Translation: EAW68392.1.
CH471064 Genomic DNA. Translation: EAW68394.1.
BC019249 mRNA. Translation: AAH19249.3.
BC064388 mRNA. Translation: AAH64388.1.
BC090043 mRNA. Translation: AAH90043.1.
CCDSiCCDS7840.1.
PIRiA30933. DEHULC.
RefSeqiNP_002292.1. NM_002301.4.
NP_059144.1. NM_017448.3.
UniGeneiHs.654377.

3D structure databases

ProteinModelPortaliP07864.
SMRiP07864. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110140. 7 interactions.
STRINGi9606.ENSP00000280704.

PTM databases

PhosphoSiteiP07864.

Polymorphism and mutation databases

BioMutaiLDHC.
DMDMi76363520.

Proteomic databases

MaxQBiP07864.
PaxDbiP07864.
PRIDEiP07864.

Protocols and materials databases

DNASUi3948.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000280704; ENSP00000280704; ENSG00000166796.
ENST00000541669; ENSP00000437783; ENSG00000166796.
GeneIDi3948.
KEGGihsa:3948.
UCSCiuc001mom.4. human.

Organism-specific databases

CTDi3948.
GeneCardsiGC11P018433.
HGNCiHGNC:6544. LDHC.
HPAiHPA045442.
MIMi150150. gene.
neXtProtiNX_P07864.
PharmGKBiPA30328.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0039.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP07864.
KOiK00016.
OMAiKNSQCKI.
OrthoDBiEOG7X0VH3.
PhylomeDBiP07864.
TreeFamiTF314963.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Miscellaneous databases

ChiTaRSiLDHC. human.
GenomeRNAii3948.
NextBioi15487.
PROiP07864.
SOURCEiSearch...

Gene expression databases

BgeeiP07864.
CleanExiHS_LDHC.
ExpressionAtlasiP07864. baseline and differential.
GenevisibleiP07864. HS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence."
    Millan J.L., Driscoll C.E., Goldberg E.
    Proc. Natl. Acad. Sci. U.S.A. 84:5311-5315(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "Human testicular lactate dehydrogenase-C gene is interrupted by six introns at positions homologous to those of LDH-A (muscle) and LDH-B (heart) genes."
    Takano T., Li S.S.-L.
    Biochem. Biophys. Res. Commun. 159:579-583(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Human testicular lactate dehydrogenase-C gene: cDNA sequence and putative alternative splicing at the 5' noncoding region."
    Wu K., Li S.S.-L.
    J. Genet. Mol. Biol. 1:72-76(1990)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "A spermatogenesis-related gene expression profile in human spermatozoa and its potential clinical applications."
    Wang H., Zhou Z., Xu M., Li J., Xiao J., Xu Z.-Y., Sha J.
    J. Mol. Med. 82:317-324(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION.
    Tissue: Testis.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Testis.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiLDHC_HUMAN
AccessioniPrimary (citable) accession number: P07864
Secondary accession number(s): D3DQY4, Q6GSG8, Q7Z7J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 160 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.