P07860 (RPSF_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase sigma-F factor

Alternative name(s):
Sporulation sigma factor
Stage II sporulation protein AC

Gene names

Name:	sigF
Synonyms:	spoIIAC
Ordered Locus Names:	BSU23450

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. Interaction with SpoIIAB inhibits sigma F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma F activity is released in the prespore.
Developmental stage	Produced in the predivisional sporangium, but does not become active in directing gene expression until after septum formation when its activity is restricted to the forespore.
Sequence similarities	Belongs to the sigma-70 factor family.
Sequence caution	The sequence AAA22791.1 differs from that shown. Reason: Frameshift at position 180.

Ontologies

Keywords
Biological process	Sporulation Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Sigma factor
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, initiation Inferred from electronic annotation. Source: InterPro sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro sigma factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	RNA polymerase sigma-F factor		PRO_0000093943
Regions
	DNA binding	221 – 240	20	H-T-H motif By similarity
	Motif	61 – 74	14	Polymerase core binding Potential

Sequences

	Sequence		Length	Mass (Da)
	P07860 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 68970E159A1FE7D0	FASTA	255	29,372
	10 20 30 40 50 60 MDVEVKKNGK NAQLKDHEVK ELIKQSQNGD QQARDLLIEK NMRLVWSVVQ RFLNRGYEPD 70 80 90 100 110 120 DLFQIGCIGL LKSVDKFDLT YDVRFSTYAV PMIIGEIQRF IRDDGTVKVS RSLKELGNKI 130 140 150 160 170 180 RRAKDELSKT LGRVPTVQEI ADHLEIEAED VVLAQEAVRA PSSIHETVYE NDGDPITLLD 190 200 210 220 230 240 QIADNSEEKW FDKIALKEAI SDLEEREKLI VYLRYYKDQT QSEVAERLGI SQVQVSRLEK 250 KILKQIKVQM DHTDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and function in a Bacillus subtilis sporulation-specific sigma factor: molecular nature of mutations in spoIIAC." Yudkin M.D. J. Gen. Microbiol. 133:475-481(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis." Fort P., Piggot P.J. J. Gen. Microbiol. 130:2147-2153(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[4]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[5]	"Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase." Min K.-T., Hilditch C.M., Diederich B., Errington J., Yudkin M.D. Cell 74:735-742(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10. Strain: CU267.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15744 Genomic DNA. Translation: AAA22788.1. M17643 Genomic DNA. Translation: AAA22791.1. Frameshift. D84432 Genomic DNA. Translation: BAA12655.1. AL009126 Genomic DNA. Translation: CAB14277.1.
PIR	A28567.
RefSeq	NP_390226.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P07860.
SMR	P07860. Positions 28-252.
ModBase	Search...
Protein-protein interaction databases
STRING	224308.BSU23450.
Proteomic databases
PaxDb	P07860.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB14277; CAB14277; BSU23450.
GeneID	938729.
KEGG	bsu:BSU23450.
PATRIC	18976509. VBIBacSub10457_2445.
Organism-specific databases
GenoList	BSU23450. [Micado]
Phylogenomic databases
eggNOG	COG1191.
HOGENOM	HOG000270270.
KO	K03091.
OMA	IRRSQEG.
ProtClustDB	PRK05572.
Enzyme and pathway databases
BioCyc	BSUB:BSU23450-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 2 hits.
InterPro	IPR014284. RNA_pol_sigma-70_dom. IPR014322. RNA_pol_sigma-B/F/G. IPR014236. RNA_pol_sigma-F. IPR000943. RNA_pol_sigma70. IPR007627. RNA_pol_sigma70_r2. IPR007624. RNA_pol_sigma70_r3. IPR007630. RNA_pol_sigma70_r4. IPR013325. RNA_pol_sigma_r2. IPR013324. RNA_pol_sigma_r3_r4. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF04542. Sigma70_r2. 1 hit. PF04539. Sigma70_r3. 1 hit. PF04545. Sigma70_r4. 1 hit. [Graphical view]
PRINTS	PR00046. SIGMA70FCT.
SUPFAM	SSF88946. Sigma_r2. 1 hit. SSF88659. Sigma_r3_r4. 2 hits.
TIGRFAMs	TIGR02980. SigBFG. 1 hit. TIGR02937. sigma70-ECF. 1 hit. TIGR02885. spore_sigF. 1 hit.
PROSITE	PS00715. SIGMA70_1. 1 hit. PS00716. SIGMA70_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPSF_BACSU

Accession

Primary (citable) accession number: P07860

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program