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P07860

- RPSF_BACSU

UniProt

P07860 - RPSF_BACSU

Protein

RNA polymerase sigma-F factor

Gene

sigF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. Interaction with SpoIIAB inhibits sigma F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma F activity is released in the prespore.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi221 – 24020H-T-H motifBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding transcription factor activity Source: InterPro
    3. sigma factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. DNA-templated transcription, initiation Source: InterPro
    2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Sigma factor

    Keywords - Biological processi

    Sporulation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU23450-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase sigma-F factor
    Alternative name(s):
    Sporulation sigma factor
    Stage II sporulation protein AC
    Gene namesi
    Name:sigF
    Synonyms:spoIIAC
    Ordered Locus Names:BSU23450
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU23450. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 255255RNA polymerase sigma-F factorPRO_0000093943Add
    BLAST

    Proteomic databases

    PaxDbiP07860.

    Expressioni

    Developmental stagei

    Produced in the predivisional sporangium, but does not become active in directing gene expression until after septum formation when its activity is restricted to the forespore.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU23450.

    Structurei

    3D structure databases

    ProteinModelPortaliP07860.
    SMRiP07860. Positions 28-252.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi61 – 7414Polymerase core bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the sigma-70 factor family.Curated

    Phylogenomic databases

    eggNOGiCOG1191.
    HOGENOMiHOG000270270.
    KOiK03091.
    OMAiKNMRLVW.
    OrthoDBiEOG6TBHDX.
    PhylomeDBiP07860.

    Family and domain databases

    Gene3Di1.10.10.10. 2 hits.
    InterProiIPR014284. RNA_pol_sigma-70_dom.
    IPR014322. RNA_pol_sigma-B/F/G.
    IPR014236. RNA_pol_sigma-F.
    IPR000943. RNA_pol_sigma70.
    IPR007627. RNA_pol_sigma70_r2.
    IPR007624. RNA_pol_sigma70_r3.
    IPR007630. RNA_pol_sigma70_r4.
    IPR013325. RNA_pol_sigma_r2.
    IPR013324. RNA_pol_sigma_r3_r4.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF04542. Sigma70_r2. 1 hit.
    PF04539. Sigma70_r3. 1 hit.
    PF04545. Sigma70_r4. 1 hit.
    [Graphical view]
    PRINTSiPR00046. SIGMA70FCT.
    SUPFAMiSSF88659. SSF88659. 2 hits.
    SSF88946. SSF88946. 1 hit.
    TIGRFAMsiTIGR02980. SigBFG. 1 hit.
    TIGR02937. sigma70-ECF. 1 hit.
    TIGR02885. spore_sigF. 1 hit.
    PROSITEiPS00715. SIGMA70_1. 1 hit.
    PS00716. SIGMA70_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07860-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVEVKKNGK NAQLKDHEVK ELIKQSQNGD QQARDLLIEK NMRLVWSVVQ    50
    RFLNRGYEPD DLFQIGCIGL LKSVDKFDLT YDVRFSTYAV PMIIGEIQRF 100
    IRDDGTVKVS RSLKELGNKI RRAKDELSKT LGRVPTVQEI ADHLEIEAED 150
    VVLAQEAVRA PSSIHETVYE NDGDPITLLD QIADNSEEKW FDKIALKEAI 200
    SDLEEREKLI VYLRYYKDQT QSEVAERLGI SQVQVSRLEK KILKQIKVQM 250
    DHTDG 255
    Length:255
    Mass (Da):29,372
    Last modified:August 1, 1988 - v1
    Checksum:i68970E159A1FE7D0
    GO

    Sequence cautioni

    The sequence AAA22791.1 differs from that shown. Reason: Frameshift at position 180.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15744 Genomic DNA. Translation: AAA22788.1.
    M17643 Genomic DNA. Translation: AAA22791.1. Frameshift.
    D84432 Genomic DNA. Translation: BAA12655.1.
    AL009126 Genomic DNA. Translation: CAB14277.1.
    PIRiA28567.
    RefSeqiNP_390226.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB14277; CAB14277; BSU23450.
    GeneIDi938729.
    KEGGibsu:BSU23450.
    PATRICi18976509. VBIBacSub10457_2445.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15744 Genomic DNA. Translation: AAA22788.1 .
    M17643 Genomic DNA. Translation: AAA22791.1 . Frameshift.
    D84432 Genomic DNA. Translation: BAA12655.1 .
    AL009126 Genomic DNA. Translation: CAB14277.1 .
    PIRi A28567.
    RefSeqi NP_390226.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P07860.
    SMRi P07860. Positions 28-252.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU23450.

    Proteomic databases

    PaxDbi P07860.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14277 ; CAB14277 ; BSU23450 .
    GeneIDi 938729.
    KEGGi bsu:BSU23450.
    PATRICi 18976509. VBIBacSub10457_2445.

    Organism-specific databases

    GenoListi BSU23450. [Micado ]

    Phylogenomic databases

    eggNOGi COG1191.
    HOGENOMi HOG000270270.
    KOi K03091.
    OMAi KNMRLVW.
    OrthoDBi EOG6TBHDX.
    PhylomeDBi P07860.

    Enzyme and pathway databases

    BioCyci BSUB:BSU23450-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.10. 2 hits.
    InterProi IPR014284. RNA_pol_sigma-70_dom.
    IPR014322. RNA_pol_sigma-B/F/G.
    IPR014236. RNA_pol_sigma-F.
    IPR000943. RNA_pol_sigma70.
    IPR007627. RNA_pol_sigma70_r2.
    IPR007624. RNA_pol_sigma70_r3.
    IPR007630. RNA_pol_sigma70_r4.
    IPR013325. RNA_pol_sigma_r2.
    IPR013324. RNA_pol_sigma_r3_r4.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF04542. Sigma70_r2. 1 hit.
    PF04539. Sigma70_r3. 1 hit.
    PF04545. Sigma70_r4. 1 hit.
    [Graphical view ]
    PRINTSi PR00046. SIGMA70FCT.
    SUPFAMi SSF88659. SSF88659. 2 hits.
    SSF88946. SSF88946. 1 hit.
    TIGRFAMsi TIGR02980. SigBFG. 1 hit.
    TIGR02937. sigma70-ECF. 1 hit.
    TIGR02885. spore_sigF. 1 hit.
    PROSITEi PS00715. SIGMA70_1. 1 hit.
    PS00716. SIGMA70_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and function in a Bacillus subtilis sporulation-specific sigma factor: molecular nature of mutations in spoIIAC."
      Yudkin M.D.
      J. Gen. Microbiol. 133:475-481(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis."
      Fort P., Piggot P.J.
      J. Gen. Microbiol. 130:2147-2153(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
      Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
      Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    5. "Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase."
      Min K.-T., Hilditch C.M., Diederich B., Errington J., Yudkin M.D.
      Cell 74:735-742(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-10.
      Strain: CU267.

    Entry informationi

    Entry nameiRPSF_BACSU
    AccessioniPrimary (citable) accession number: P07860
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3