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Protein

RNA polymerase sigma-F factor

Gene

sigF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes (PubMed:8759874). Interaction with SpoIIAB inhibits sigma-F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma F activity is released in the prespore. Responsible for expression of csfB (the anti-sigma-G factor Gin) (PubMed:8759874).1 Publication

Enzyme regulationi

Interaction with SpoIIAB inhibits sigma-F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma-F activity is released in the prespore (PubMed:8358793). Fin, a second, forespore-specific anti-sigma factor is induced in 2 successive waves by sigma-F and sigma-G, by antagonizing sigma-F it allows the switch to sigma-G factor and progression to the late sporulation development stages (PubMed:21037003).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi221 – 240H-T-H motifBy similarityAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Sigma factor
Biological processSporulation, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciBSUB:BSU23450-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-F factor
Alternative name(s):
Sporulation sigma factor
Stage II sporulation protein AC
Gene namesi
Name:sigF
Synonyms:spoIIAC
Ordered Locus Names:BSU23450
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

In spo-63 (loss of residues 27-255); no spores develop, no induction of sporulation-associated enzymes.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73S → F in spo-563; 10(6)-fold decrease in spore production, 50% induction of sporulation-associated enzymes. 1 Publication1
Mutagenesisi99 – 255Missing in spo-578; no spores develop, no induction of sporulation-associated enzymes. 1 PublicationAdd BLAST157
Mutagenesisi111R → Q in spo-564; 1000-fold decrease in spore production, 50% induction of sporulation-associated enzymes. 1 Publication1
Mutagenesisi233V → M in spo-561; 10(6)-fold decrease in spore production, 50% induction of sporulation-associated enzymes. 1 Publication1
Mutagenesisi237R → K in spo-560; 1000-fold decrease in spore production, 50% induction of sporulation-associated enzymes. 1 Publication1
Mutagenesisi245 – 255Missing in spo-1; no spores develop, no induction of sporulation-associated enzymes. 1 PublicationAdd BLAST11

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000939431 – 255RNA polymerase sigma-F factorAdd BLAST255

Proteomic databases

PaxDbiP07860

Expressioni

Developmental stagei

Produced in the predivisional sporangium, but does not become active in directing gene expression until after septum formation when its activity is restricted to the forespore (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012871

Structurei

3D structure databases

ProteinModelPortaliP07860
SMRiP07860
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 74Polymerase core bindingAdd BLAST14

Sequence similaritiesi

Belongs to the sigma-70 factor family.Curated

Phylogenomic databases

eggNOGiENOG4105CT2 Bacteria
COG1191 LUCA
HOGENOMiHOG000270270
InParanoidiP07860
KOiK03091
OMAiMNLPRFP
PhylomeDBiP07860

Family and domain databases

Gene3Di1.10.10.10, 2 hits
InterProiView protein in InterPro
IPR014284 RNA_pol_sigma-70_dom
IPR014322 RNA_pol_sigma-B/F/G
IPR014236 RNA_pol_sigma-F
IPR000943 RNA_pol_sigma70
IPR007627 RNA_pol_sigma70_r2
IPR007624 RNA_pol_sigma70_r3
IPR007630 RNA_pol_sigma70_r4
IPR013325 RNA_pol_sigma_r2
IPR013324 RNA_pol_sigma_r3/r4-like
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF04542 Sigma70_r2, 1 hit
PF04539 Sigma70_r3, 1 hit
PF04545 Sigma70_r4, 1 hit
PIRSFiPIRSF000770 RNA_pol_sigma-SigE/K, 1 hit
PRINTSiPR00046 SIGMA70FCT
SUPFAMiSSF88659 SSF88659, 2 hits
SSF88946 SSF88946, 1 hit
TIGRFAMsiTIGR02980 SigBFG, 1 hit
TIGR02937 sigma70-ECF, 1 hit
TIGR02885 spore_sigF, 1 hit
PROSITEiView protein in PROSITE
PS00715 SIGMA70_1, 1 hit
PS00716 SIGMA70_2, 1 hit

Sequencei

Sequence statusi: Complete.

P07860-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVEVKKNGK NAQLKDHEVK ELIKQSQNGD QQARDLLIEK NMRLVWSVVQ
60 70 80 90 100
RFLNRGYEPD DLFQIGCIGL LKSVDKFDLT YDVRFSTYAV PMIIGEIQRF
110 120 130 140 150
IRDDGTVKVS RSLKELGNKI RRAKDELSKT LGRVPTVQEI ADHLEIEAED
160 170 180 190 200
VVLAQEAVRA PSSIHETVYE NDGDPITLLD QIADNSEEKW FDKIALKEAI
210 220 230 240 250
SDLEEREKLI VYLRYYKDQT QSEVAERLGI SQVQVSRLEK KILKQIKVQM

DHTDG
Length:255
Mass (Da):29,372
Last modified:August 1, 1988 - v1
Checksum:i68970E159A1FE7D0
GO

Sequence cautioni

The sequence AAA22791 differs from that shown. Reason: Frameshift at position 180.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15744 Genomic DNA Translation: AAA22788.1
M17643 Genomic DNA Translation: AAA22791.1 Frameshift.
D84432 Genomic DNA Translation: BAA12655.1
AL009126 Genomic DNA Translation: CAB14277.1
PIRiA28567
RefSeqiNP_390226.1, NC_000964.3
WP_003230458.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14277; CAB14277; BSU23450
GeneIDi938729
KEGGibsu:BSU23450
PATRICifig|224308.179.peg.2555

Similar proteinsi

Entry informationi

Entry nameiRPSF_BACSU
AccessioniPrimary (citable) accession number: P07860
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: April 25, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health