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Protein

RNA polymerase sigma-F factor

Gene

sigF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. Interaction with SpoIIAB inhibits sigma F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma F activity is released in the prespore.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi221 – 24020H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
  3. sigma factor activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA-templated transcription, initiation Source: InterPro
  2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Sigma factor

Keywords - Biological processi

Sporulation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU23450-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-F factor
Alternative name(s):
Sporulation sigma factor
Stage II sporulation protein AC
Gene namesi
Name:sigF
Synonyms:spoIIAC
Ordered Locus Names:BSU23450
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU23450. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255RNA polymerase sigma-F factorPRO_0000093943Add
BLAST

Proteomic databases

PaxDbiP07860.

Expressioni

Developmental stagei

Produced in the predivisional sporangium, but does not become active in directing gene expression until after septum formation when its activity is restricted to the forespore.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU23450.

Structurei

3D structure databases

ProteinModelPortaliP07860.
SMRiP07860. Positions 28-252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7414Polymerase core bindingAdd
BLAST

Sequence similaritiesi

Belongs to the sigma-70 factor family.Curated

Phylogenomic databases

eggNOGiCOG1191.
HOGENOMiHOG000270270.
InParanoidiP07860.
KOiK03091.
OMAiFNLVQRF.
OrthoDBiEOG6TBHDX.
PhylomeDBiP07860.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR014322. RNA_pol_sigma-B/F/G.
IPR014236. RNA_pol_sigma-F.
IPR000943. RNA_pol_sigma70.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02980. SigBFG. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
TIGR02885. spore_sigF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07860-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVEVKKNGK NAQLKDHEVK ELIKQSQNGD QQARDLLIEK NMRLVWSVVQ
60 70 80 90 100
RFLNRGYEPD DLFQIGCIGL LKSVDKFDLT YDVRFSTYAV PMIIGEIQRF
110 120 130 140 150
IRDDGTVKVS RSLKELGNKI RRAKDELSKT LGRVPTVQEI ADHLEIEAED
160 170 180 190 200
VVLAQEAVRA PSSIHETVYE NDGDPITLLD QIADNSEEKW FDKIALKEAI
210 220 230 240 250
SDLEEREKLI VYLRYYKDQT QSEVAERLGI SQVQVSRLEK KILKQIKVQM

DHTDG
Length:255
Mass (Da):29,372
Last modified:August 1, 1988 - v1
Checksum:i68970E159A1FE7D0
GO

Sequence cautioni

The sequence AAA22791.1 differs from that shown. Reason: Frameshift at position 180. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15744 Genomic DNA. Translation: AAA22788.1.
M17643 Genomic DNA. Translation: AAA22791.1. Frameshift.
D84432 Genomic DNA. Translation: BAA12655.1.
AL009126 Genomic DNA. Translation: CAB14277.1.
PIRiA28567.
RefSeqiNP_390226.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14277; CAB14277; BSU23450.
GeneIDi938729.
KEGGibsu:BSU23450.
PATRICi18976509. VBIBacSub10457_2445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15744 Genomic DNA. Translation: AAA22788.1.
M17643 Genomic DNA. Translation: AAA22791.1. Frameshift.
D84432 Genomic DNA. Translation: BAA12655.1.
AL009126 Genomic DNA. Translation: CAB14277.1.
PIRiA28567.
RefSeqiNP_390226.1. NC_000964.3.

3D structure databases

ProteinModelPortaliP07860.
SMRiP07860. Positions 28-252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU23450.

Proteomic databases

PaxDbiP07860.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14277; CAB14277; BSU23450.
GeneIDi938729.
KEGGibsu:BSU23450.
PATRICi18976509. VBIBacSub10457_2445.

Organism-specific databases

GenoListiBSU23450. [Micado]

Phylogenomic databases

eggNOGiCOG1191.
HOGENOMiHOG000270270.
InParanoidiP07860.
KOiK03091.
OMAiFNLVQRF.
OrthoDBiEOG6TBHDX.
PhylomeDBiP07860.

Enzyme and pathway databases

BioCyciBSUB:BSU23450-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR014322. RNA_pol_sigma-B/F/G.
IPR014236. RNA_pol_sigma-F.
IPR000943. RNA_pol_sigma70.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02980. SigBFG. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
TIGR02885. spore_sigF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and function in a Bacillus subtilis sporulation-specific sigma factor: molecular nature of mutations in spoIIAC."
    Yudkin M.D.
    J. Gen. Microbiol. 133:475-481(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis."
    Fort P., Piggot P.J.
    J. Gen. Microbiol. 130:2147-2153(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
    Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
    Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  5. "Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase."
    Min K.-T., Hilditch C.M., Diederich B., Errington J., Yudkin M.D.
    Cell 74:735-742(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10.
    Strain: CU267.

Entry informationi

Entry nameiRPSF_BACSU
AccessioniPrimary (citable) accession number: P07860
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: January 7, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.