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Protein

Acyl carrier protein 1, chloroplastic

Gene

ACL1.1

Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Carrier of the growing fatty acid chain in fatty acid biosynthesis.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl carrier protein 1, chloroplastic
Short name:
ACP I
Short name:
Acyl carrier protein I
Gene namesi
Name:ACL1.1
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5656Chloroplast1 PublicationAdd
BLAST
Chaini57 – 13882Acyl carrier protein 1, chloroplasticPRO_0000000584Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei94 – 941O-(pantetheine 4'-phosphoryl)serine1 Publication

Post-translational modificationi

4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by acpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group (By similarity).By similarity

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Protein-protein interaction databases

DIPiDIP-60378N.

Structurei

Secondary structure

1
138
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi59 – 635Combined sources
Helixi66 – 716Combined sources
Turni72 – 743Combined sources
Beta strandi77 – 793Combined sources
Beta strandi83 – 853Combined sources
Helixi87 – 904Combined sources
Helixi95 – 1039Combined sources
Turni104 – 1074Combined sources
Turni114 – 1185Combined sources
Beta strandi121 – 1233Combined sources
Helixi124 – 13310Combined sources
Turni135 – 1373Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AVANMR-A57-138[»]
2FVANMR-A57-138[»]
2FVENMR-A57-138[»]
2FVFNMR-A57-138[»]
2XZ0X-ray3.00D57-138[»]
2XZ1X-ray3.35C/D57-138[»]
ProteinModelPortaliP07854.
SMRiP07854. Positions 57-138.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07854.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni72 – 732Phosphopantetheine binding
Regioni88 – 936Phosphopantetheine binding
Regioni95 – 995Phosphopantetheine binding
Regioni118 – 1192Phosphopantetheine binding

Sequence similaritiesi

Contains 1 acyl carrier domain.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
HAMAPiMF_01217. Acyl_carrier.
InterProiIPR003231. Acyl_carrier.
IPR020806. PKS_PP-bd.
IPR009081. PP-bd_ACP.
IPR006162. Ppantetheine_attach_site.
[Graphical view]
PfamiPF00550. PP-binding. 1 hit.
[Graphical view]
ProDomiPD000887. PD000887. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00823. PKS_PP. 1 hit.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 1 hit.
TIGRFAMsiTIGR00517. acyl_carrier. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07854-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLSATTTV RVQPSSSSLH KLSQGNGRCS SIVCLDWGKS SFPTLRTSRR
60 70 80 90 100
RSFISAAKKE TIDKVCDIVK EKLALGADVV VTADSEFSKL GADSLDTVEI
110 120 130
VMNLEEEFGI NVDEDKAQDI STIQQAADVI ESLLEKKA
Length:138
Mass (Da):14,909
Last modified:October 1, 1989 - v2
Checksum:iB3FB8F08BF657980
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661C → S AA sequence (PubMed:6486822).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17636 mRNA. Translation: AAA34023.1.
PIRiA28052. AYSP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17636 mRNA. Translation: AAA34023.1.
PIRiA28052. AYSP.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AVANMR-A57-138[»]
2FVANMR-A57-138[»]
2FVENMR-A57-138[»]
2FVFNMR-A57-138[»]
2XZ0X-ray3.00D57-138[»]
2XZ1X-ray3.35C/D57-138[»]
ProteinModelPortaliP07854.
SMRiP07854. Positions 57-138.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60378N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00094.

Miscellaneous databases

EvolutionaryTraceiP07854.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
HAMAPiMF_01217. Acyl_carrier.
InterProiIPR003231. Acyl_carrier.
IPR020806. PKS_PP-bd.
IPR009081. PP-bd_ACP.
IPR006162. Ppantetheine_attach_site.
[Graphical view]
PfamiPF00550. PP-binding. 1 hit.
[Graphical view]
ProDomiPD000887. PD000887. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00823. PKS_PP. 1 hit.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 1 hit.
TIGRFAMsiTIGR00517. acyl_carrier. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of a cDNA clone for the acyl carrier protein-I of spinach."
    Scherer D.E., Knauf V.C.
    Plant Mol. Biol. 9:127-134(1987)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The primary structure of spinach acyl carrier protein."
    Kuo T.M., Ohlrogge J.B.
    Arch. Biochem. Biophys. 234:290-296(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 57-138.
    Tissue: Leaf.
  3. "Solution structures of spinach acyl carrier protein with decanoate and stearate."
    Zornetzer G.A., Fox B.G., Markley J.L.
    Biochemistry 45:5217-5227(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 57-138 IN COMPLEX WITH PHOSPHOPANTETHEINE, PHOSPHOPANTETHEINYLATION AT SER-94.

Entry informationi

Entry nameiACP1_SPIOL
AccessioniPrimary (citable) accession number: P07854
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1989
Last modified: June 8, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.