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P07846

- DHSO_SHEEP

UniProt

P07846 - DHSO_SHEEP

Protein

Sorbitol dehydrogenase

Gene

SORD

Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm By similarity.By similarity

    Catalytic activityi

    L-iditol + NAD+ = L-sorbose + NADH.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi43 – 431Zinc; catalytic
    Binding sitei49 – 491Substrate
    Metal bindingi67 – 671Zinc; catalytic
    Metal bindingi68 – 681Zinc; catalytic
    Binding sitei153 – 1531Substrate
    Binding sitei296 – 2961Substrate
    Binding sitei297 – 2971Substrate

    GO - Molecular functioni

    1. L-iditol 2-dehydrogenase activity Source: UniProtKB
    2. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. sperm motility Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Metal-binding, NAD, Zinc

    Enzyme and pathway databases

    SABIO-RKP07846.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sorbitol dehydrogenase (EC:1.1.1.14)
    Alternative name(s):
    L-iditol 2-dehydrogenase
    Gene namesi
    Name:SORD
    OrganismiOvis aries (Sheep)
    Taxonomic identifieri9940 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
    ProteomesiUP000002356: Unplaced

    Subcellular locationi

    Mitochondrion membrane; Peripheral membrane protein. Cell projectionciliumflagellum
    Note: Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface By similarity.By similarity

    GO - Cellular componenti

    1. mitochondrial membrane Source: UniProtKB-SubCell
    2. motile cilium Source: UniProtKB

    Keywords - Cellular componenti

    Cell projection, Cilium, Flagellum, Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 354354Sorbitol dehydrogenasePRO_0000160819Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylalanine

    Keywords - PTMi

    Acetylation

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Structurei

    Secondary structure

    1
    354
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 147
    Beta strandi17 – 226
    Beta strandi32 – 4211
    Helixi44 – 518
    Beta strandi52 – 543
    Beta strandi68 – 769
    Beta strandi88 – 914
    Beta strandi93 – 953
    Helixi101 – 1044
    Helixi108 – 1103
    Beta strandi127 – 1337
    Helixi134 – 1363
    Beta strandi137 – 1393
    Helixi146 – 16520
    Beta strandi172 – 1765
    Helixi180 – 19112
    Beta strandi195 – 2028
    Helixi204 – 21310
    Beta strandi216 – 2205
    Helixi226 – 23712
    Beta strandi242 – 2465
    Helixi251 – 26010
    Beta strandi266 – 2694
    Helixi281 – 2866
    Beta strandi290 – 2934
    Helixi301 – 3099
    Helixi316 – 3183
    Beta strandi319 – 3246
    Helixi325 – 3273
    Helixi328 – 33710
    Beta strandi339 – 3468

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SDGmodel-A1-347[»]
    3QE3X-ray1.90A1-354[»]
    ProteinModelPortaliP07846.
    SMRiP07846. Positions 1-354.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07846.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOVERGENiHBG005484.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProiIPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11695. PTHR11695. 1 hit.
    PfamiPF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50129. SSF50129. 1 hit.
    PROSITEiPS00059. ADH_ZINC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07846-1 [UniParc]FASTAAdd to Basket

    « Hide

    AKPAAENLSL VVHGPGDLRL ENYPIPEPGP NEVLLKMHSV GICGSDVHYW    50
    QGRIGDFVVK KPMVLGHEAS GTVVKVGSLV RHLQPGDRVA IQPGAPRQTD 100
    EFCKIGRYNL SPTIFFCATP PDDGNLCRFY KHNANFCYKL PDNVTFEEGA 150
    LIEPLSVGIH ACRRAGVTLG NKVLVCGAGP IGLVNLLAAK AMGAAQVVVT 200
    DLSASRLSKA KEVGADFILE ISNESPEEIA KKVEGLLGSK PEVTIECTGV 250
    ETSIQAGIYA THSGGTLVLV GLGSEMTSVP LVHAATREVD IKGVFRYCNT 300
    WPMAISMLAS KSVNVKPLVT HRFPLEKALE AFETSKKGLG LKVMIKCDPS 350
    DQNP 354
    Length:354
    Mass (Da):37,831
    Last modified:August 1, 1988 - v1
    Checksum:iD5FE56BC06F87389
    GO

    Sequence databases

    PIRiS10065.

    Cross-referencesi

    Sequence databases

    PIRi S10065.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SDG model - A 1-347 [» ]
    3QE3 X-ray 1.90 A 1-354 [» ]
    ProteinModelPortali P07846.
    SMRi P07846. Positions 1-354.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1075154.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG005484.

    Enzyme and pathway databases

    SABIO-RK P07846.

    Miscellaneous databases

    EvolutionaryTracei P07846.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProi IPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11695. PTHR11695. 1 hit.
    Pfami PF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50129. SSF50129. 1 hit.
    PROSITEi PS00059. ADH_ZINC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sorbitol dehydrogenase. The primary structure of the sheep-liver enzyme."
      Jeffery J., Cederlund E., Joernvall H.
      Eur. J. Biochem. 140:7-16(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Tissue: Liver.
    2. "Extensive variations and basic features in the alcohol dehydrogenase-sorbitol dehydrogenase family."
      Joernvall H., von Bahr-Lindstroem H., Jeffery J.
      Eur. J. Biochem. 140:17-23(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIMILARITY TO ZINC ALCOHOL DEHYDROGENASES.
    3. "Molecular aspects of functional differences between alcohol and sorbitol dehydrogenases."
      Eklund H., Horjales E., Joernvall H., Branden C.I., Jeffery J.
      Biochemistry 24:8005-8012(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.
    4. "X-ray crystal structure and small-angle X-ray scattering of sheep liver sorbitol dehydrogenase."
      Yennawar H., Moller M., Gillilan R., Yennawar N.
      Acta Crystallogr. D 67:440-446(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG, SUBUNIT, ZINC-BINDING SITES, SUBSTRATE-BINDING SITES, ENZYME MECHANISM.

    Entry informationi

    Entry nameiDHSO_SHEEP
    AccessioniPrimary (citable) accession number: P07846
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3