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Reviewed, UniProtKB/Swiss-Prot P07834 (CDC4_YEAST)

Last modified September 2, 2008. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cell division control protein 4
Alternative name(s):
    F-box protein CDC4
    E3 ubiquitin ligase complex SCF subunit CDC4
Gene names
Name: CDC4
Ordered Locus Names: YFL009W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length779 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. Directs ubiquitination of the phosphorylated CDK inhibitor SIC1. Involved in the degradation of CDC6 together with CDC34/UBC3 and CDC53, and in restricting the degradation of FAR1 to the nucleus. Is essential for initiation of DNA replication and separation of the spindle pole bodies to form the poles of the mitotic spindle. It also plays a role in bud development, fusion of zygotic nuclei after conjugation and various aspects of sporulation. Required for HTA1-HTB1 locus transcription activation. Required for G1/S and G2/M transition.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with DCD53 and SKP1. Component of the SCF(CDC4) complex containing CDC53, SKP1, RBX1 and CDC4. CDC34. Interacts with CDC6 and CIC1. Interacts with SIC1; the interaction involves a SIC1 double phosphorylated motif (degron). Homodimerizes; the dimerization increases SIC1 ubiquitination in vitro.

Subcellular location

Nucleus.

Sequence similarities

Contains 1 F-box domain.

Contains 7 WD repeats.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 779779Cell division control protein 4

Regions

Domain272 – 31948F-box
Repeat380 – 40829WD 1
Repeat420 – 44930WD 2
Repeat461 – 49333WD 3
Repeat528 – 55629WD 4
Repeat568 – 59831WD 5
Repeat630 – 65829WD 6
Repeat669 – 69830WD 7
Motif82 – 854Nuclear localization signal

Amino acid modifications

Modified residue31Phosphoserine
Modified residue311Phosphoserine

Experimental info

Mutagenesis821K → A: Prevents nuclear localization; when associated with A-83 and A-85
Mutagenesis831R → A: Prevents nuclear localization; when associated with A-82 and A-85
Mutagenesis831R → G: Prevents nuclear localization
Mutagenesis851K → A: Prevents nuclear localization; when associated with A-82 and A-83
Sequence conflict4601K → E in CAA29113. Ref.1

Secondary structure

............................................................................................... 779
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07834-1 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 0348F2F8FA78F3BC

FASTA77986,090
        10         20         30         40         50         60 
MGSFPLAEFP LRDIPVPYSY RVSGGIASSG SVTALVTAAG THRNSSTAKT VETEDGEEDI 

        70         80         90        100        110        120 
DEYQRKRAAG SGESTPERSD FKRVKHDNHK TLHPVNLQNT GAASVDNDGL HNLTDISNDA 

       130        140        150        160        170        180 
EKLLMSVDDG SAAPSTLSVN MGVASHNVAA PTTVNAATIT GSDVSNNVNS ATINNPMEEG 

       190        200        210        220        230        240 
ALPLSPTASS PGTTTPLAKT TKTINNNNNI ADLIESKDSI ISPEYLSDEI FSAINNNLPH 

       250        260        270        280        290        300 
AYFKNLLFRL VANMDRSELS DLGTLIKDNL KRDLITSLPF EISLKIFNYL QFEDIINSLG 

       310        320        330        340        350        360 
VSQNWNKIIR KSTSLWKKLL ISENFVSPKG FNSLNLKLSQ KYPKLSQQDR LRLSFLENIF 

       370        380        390        400        410        420 
ILKNWYNPKF VPQRTTLRGH MTSVITCLQF EDNYVITGAD DKMIRVYDSI NKKFLLQLSG 

       430        440        450        460        470        480 
HDGGVWALKY AHGGILVSGS TDRTVRVWDI KKGCCTHVFK GHNSTVRCLD IVEYKNIKYI 

       490        500        510        520        530        540 
VTGSRDNTLH VWKLPKESSV PDHGEEHDYP LVFHTPEENP YFVGVLRGHM ASVRTVSGHG 

       550        560        570        580        590        600 
NIVVSGSYDN TLIVWDVAQM KCLYILSGHT DRIYSTIYDH ERKRCISASM DTTIRIWDLE 

       610        620        630        640        650        660 
NIWNNGECSY ATNSASPCAK ILGAMYTLQG HTALVGLLRL SDKFLVSAAA DGSIRGWDAN 

       670        680        690        700        710        720 
DYSRKFSYHH TNLSAITTFY VSDNILVSGS ENQFNIYNLR SGKLVHANIL KDADQIWSVN 

       730        740        750        760        770 
FKGKTLVAAV EKDGQSFLEI LDFSKASKIN YVSNPVNSSS SSLESISTSL GLTRTTIIP 

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References

« Hide 'large scale' references
[1]"Structural comparison of the yeast cell division cycle gene CDC4 and a related pseudogene."
Yochem J., Byers B.
J. Mol. Biol. 195:233-245(1987) [PubMed: 3309335] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]Barrell B.G., Churcher C., Rajandream M.A.
Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-579.
Strain: ATCC 204511 / S288c / AB972.
[4]"Regulated degradation of the transcription factor Gcn4."
Kornitzer D., Raboy B., Kulka R.G., Fink G.R.
EMBO J. 13:6021-6030(1994) [PubMed: 7813440] [Abstract]
Cited for: FUNCTION.
[5]"SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box."
Bai C., Sen P., Hofmann K., Ma L., Goebl M., Harper J.W., Elledge S.J.
Cell 86:263-274(1996) [PubMed: 8706131] [Abstract]
Cited for: INTERACTION WITH SKP1/CBF3D.
Strain: ATCC 204508 / S288c.
[6]"F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex."
Skowyra D., Craig K.L., Tyers M., Elledge S.J., Harper J.W.
Cell 91:209-219(1997) [PubMed: 9346238] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[7]"A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the