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Protein

Cell division control protein 4

Gene

CDC4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. Directs ubiquitination of the phosphorylated CDK inhibitor SIC1. Involved in the degradation of CDC6 together with CDC34/UBC3 and CDC53, and in restricting the degradation of FAR1 to the nucleus. Is essential for initiation of DNA replication and separation of the spindle pole bodies to form the poles of the mitotic spindle. It also plays a role in bud development, fusion of zygotic nuclei after conjugation and various aspects of sporulation. Required for HTA1-HTB1 locus transcription activation. Required for G1/S and G2/M transition.9 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • phosphoserine residue binding Source: SGD
  • ubiquitin binding Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • G1/S transition of mitotic cell cycle Source: SGD
  • G2/M transition of mitotic cell cycle Source: SGD
  • meiotic cell cycle Source: SGD
  • protein ubiquitination Source: SGD
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Sporulation, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-30447-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 4
Alternative name(s):
E3 ubiquitin ligase complex SCF subunit CDC4
F-box protein CDC4
Gene namesi
Name:CDC4
Ordered Locus Names:YFL009W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL009W.
SGDiS000001885. CDC4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi82K → A: Prevents nuclear localization; when associated with A-83 and A-85. 1
Mutagenesisi83R → A: Prevents nuclear localization; when associated with A-82 and A-85. 1
Mutagenesisi83R → G: Prevents nuclear localization. 1
Mutagenesisi85K → A: Prevents nuclear localization; when associated with A-82 and A-83. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508991 – 779Cell division control protein 4Add BLAST779

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei104PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07834.
PRIDEiP07834.

PTM databases

iPTMnetiP07834.

Interactioni

Subunit structurei

Interacts with DCD53 and SKP1. Component of the SCF(CDC4) complex containing CDC53, SKP1, RBX1 and CDC4. CDC34. Interacts with CDC6 and CIC1. Interacts with SIC1; the interaction involves a SIC1 double phosphorylated motif (degron). Homodimerizes; the dimerization increases SIC1 ubiquitination in vitro.11 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • phosphoserine residue binding Source: SGD
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi31138. 583 interactors.
DIPiDIP-1625N.
IntActiP07834. 14 interactors.
MINTiMINT-389524.
STRINGi4932.YFL009W.

Structurei

Secondary structure

1779
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi228 – 234Combined sources7
Helixi235 – 237Combined sources3
Helixi242 – 253Combined sources12
Helixi256 – 271Combined sources16
Helixi274 – 277Combined sources4
Helixi280 – 287Combined sources8
Helixi292 – 299Combined sources8
Helixi303 – 309Combined sources7
Helixi313 – 322Combined sources10
Turni328 – 330Combined sources3
Helixi331 – 341Combined sources11
Helixi347 – 366Combined sources20
Beta strandi373 – 378Combined sources6
Beta strandi381 – 383Combined sources3
Beta strandi385 – 391Combined sources7
Beta strandi394 – 399Combined sources6
Beta strandi404 – 408Combined sources5
Turni409 – 412Combined sources4
Beta strandi413 – 418Combined sources6
Beta strandi425 – 430Combined sources6
Turni432 – 434Combined sources3
Beta strandi435 – 440Combined sources6
Beta strandi445 – 449Combined sources5
Turni450 – 453Combined sources4
Beta strandi454 – 459Combined sources6
Beta strandi466 – 476Combined sources11
Beta strandi478 – 484Combined sources7
Beta strandi489 – 493Combined sources5
Beta strandi509 – 514Combined sources6
Helixi516 – 518Combined sources3
Beta strandi522 – 526Combined sources5
Beta strandi533 – 539Combined sources7
Beta strandi542 – 547Combined sources6
Beta strandi552 – 556Combined sources5
Turni557 – 560Combined sources4
Beta strandi561 – 566Combined sources6
Beta strandi573 – 579Combined sources7
Turni580 – 583Combined sources4
Beta strandi584 – 589Combined sources6
Beta strandi594 – 598Combined sources5
Turni599 – 601Combined sources3
Beta strandi625 – 628Combined sources4
Beta strandi635 – 640Combined sources6
Beta strandi642 – 649Combined sources8
Beta strandi652 – 658Combined sources7
Turni659 – 661Combined sources3
Beta strandi664 – 669Combined sources6
Beta strandi676 – 681Combined sources6
Beta strandi683 – 690Combined sources8
Beta strandi693 – 698Combined sources6
Turni699 – 701Combined sources3
Beta strandi704 – 707Combined sources4
Turni709 – 712Combined sources4
Beta strandi714 – 722Combined sources9
Beta strandi725 – 732Combined sources8
Beta strandi735 – 742Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NEXX-ray2.70B/D263-744[»]
2P63X-ray2.67A/B/C/D222-273[»]
3MKSX-ray2.60B/D263-744[»]
3V7DX-ray2.31B/D263-744[»]
ProteinModelPortaliP07834.
SMRiP07834.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07834.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini272 – 319F-boxPROSITE-ProRule annotationAdd BLAST48
Repeati380 – 408WD 1Add BLAST29
Repeati420 – 449WD 2Add BLAST30
Repeati461 – 493WD 3Add BLAST33
Repeati528 – 556WD 4Add BLAST29
Repeati568 – 598WD 5Add BLAST31
Repeati630 – 658WD 6Add BLAST29
Repeati669 – 698WD 7Add BLAST30

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi82 – 85Nuclear localization signal4

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00900000142520.
HOGENOMiHOG000111495.
InParanoidiP07834.
KOiK03361.
OMAiCLQHDDE.
OrthoDBiEOG092C172M.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR031740. Cdc4_D.
IPR036047. F-box-like_dom_sf.
IPR001810. F-box_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR036322. WD40_repeat_dom_sf.
PfamiView protein in Pfam
PF16856. CDC4_D. 1 hit.
PF12937. F-box-like. 1 hit.
PF00400. WD40. 5 hits.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiView protein in SMART
SM00256. FBOX. 1 hit.
SM00320. WD40. 7 hits.
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiView protein in PROSITE
PS50181. FBOX. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

P07834-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSFPLAEFP LRDIPVPYSY RVSGGIASSG SVTALVTAAG THRNSSTAKT
60 70 80 90 100
VETEDGEEDI DEYQRKRAAG SGESTPERSD FKRVKHDNHK TLHPVNLQNT
110 120 130 140 150
GAASVDNDGL HNLTDISNDA EKLLMSVDDG SAAPSTLSVN MGVASHNVAA
160 170 180 190 200
PTTVNAATIT GSDVSNNVNS ATINNPMEEG ALPLSPTASS PGTTTPLAKT
210 220 230 240 250
TKTINNNNNI ADLIESKDSI ISPEYLSDEI FSAINNNLPH AYFKNLLFRL
260 270 280 290 300
VANMDRSELS DLGTLIKDNL KRDLITSLPF EISLKIFNYL QFEDIINSLG
310 320 330 340 350
VSQNWNKIIR KSTSLWKKLL ISENFVSPKG FNSLNLKLSQ KYPKLSQQDR
360 370 380 390 400
LRLSFLENIF ILKNWYNPKF VPQRTTLRGH MTSVITCLQF EDNYVITGAD
410 420 430 440 450
DKMIRVYDSI NKKFLLQLSG HDGGVWALKY AHGGILVSGS TDRTVRVWDI
460 470 480 490 500
KKGCCTHVFK GHNSTVRCLD IVEYKNIKYI VTGSRDNTLH VWKLPKESSV
510 520 530 540 550
PDHGEEHDYP LVFHTPEENP YFVGVLRGHM ASVRTVSGHG NIVVSGSYDN
560 570 580 590 600
TLIVWDVAQM KCLYILSGHT DRIYSTIYDH ERKRCISASM DTTIRIWDLE
610 620 630 640 650
NIWNNGECSY ATNSASPCAK ILGAMYTLQG HTALVGLLRL SDKFLVSAAA
660 670 680 690 700
DGSIRGWDAN DYSRKFSYHH TNLSAITTFY VSDNILVSGS ENQFNIYNLR
710 720 730 740 750
SGKLVHANIL KDADQIWSVN FKGKTLVAAV EKDGQSFLEI LDFSKASKIN
760 770
YVSNPVNSSS SSLESISTSL GLTRTTIIP
Length:779
Mass (Da):86,090
Last modified:November 1, 1995 - v2
Checksum:i0348F2F8FA78F3BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti460K → E in CAA29113 (PubMed:3309335).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05625 Genomic DNA. Translation: CAA29113.1.
D50617 Genomic DNA. Translation: BAA09229.1.
Z46255 Genomic DNA. Translation: CAA86341.1.
BK006940 Genomic DNA. Translation: DAA12431.1.
PIRiS56245.
RefSeqiNP_116585.1. NM_001179957.1.

Genome annotation databases

EnsemblFungiiBAA09229; BAA09229; BAA09229.
YFL009W; YFL009W; YFL009W.
GeneIDi850539.
KEGGisce:YFL009W.

Similar proteinsi

Entry informationi

Entry nameiCDC4_YEAST
AccessioniPrimary (citable) accession number: P07834
Secondary accession number(s): D6VTM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1995
Last modified: November 22, 2017
This is version 186 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names