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Protein

Photosystem II protein D1 1

Gene

psbA1

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Photosystem II (PSII) is a light-driven water: plastoquinone oxidoreductase that uses light energy to abstract electrons from H2O, generating O2 and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1/D2 (PsbA/PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors.UniRule annotation

Catalytic activityi

2 H2O + 2 plastoquinone + 4 light = O2 + 2 plastoquinol.UniRule annotation

Cofactori

Note: The D1/D2 heterodimer binds P680, chlorophylls that are the primary electron donor of PSII, and subsequent electron acceptors. It shares a non-heme iron and each subunit binds pheophytin, quinone, additional chlorophylls, carotenoids and lipids. D1 provides most of the ligands for the Mn4-Ca-O5 cluster of the oxygen-evolving complex (OEC). There is also a Cl(-1) ion associated with D1 and D2, which is required for oxygen evolution. The PSII complex binds additional chlorophylls, carotenoids and specific lipids.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi118 – 1181Magnesium (chlorophyll-a ChlzD1 axial ligand); via tele nitrogenUniRule annotation
Binding sitei126 – 1261Pheophytin D1UniRule annotation
Sitei161 – 1611Tyrosine radical intermediateUniRule annotation
Metal bindingi170 – 1701Calcium-manganese-oxide [Ca-4Mn-5O]; calciumUniRule annotation
Metal bindingi170 – 1701Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 4UniRule annotation
Metal bindingi189 – 1891Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1UniRule annotation
Sitei190 – 1901Stabilizes free radical intermediateUniRule annotation
Metal bindingi198 – 1981Magnesium (chlorophyll-a PD1 axial ligand); via tele nitrogenUniRule annotation
Metal bindingi215 – 2151Iron; shared with heterodimeric partner; via tele nitrogenUniRule annotation
Binding sitei215 – 2151Quinone (B)UniRule annotation
Metal bindingi272 – 2721Iron; shared with heterodimeric partner; via tele nitrogenUniRule annotation
Metal bindingi332 – 3321Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1; via tele nitrogenUniRule annotation
Metal bindingi333 – 3331Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 3UniRule annotation
Metal bindingi333 – 3331Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 4UniRule annotation
Metal bindingi342 – 3421Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1UniRule annotation
Metal bindingi342 – 3421Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 2UniRule annotation
Sitei344 – 3452Cleavage; by CtpAUniRule annotation1 Publication
Metal bindingi344 – 3441Calcium-manganese-oxide [Ca-4Mn-5O]; calcium; via carboxylateUniRule annotation
Metal bindingi344 – 3441Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 2; via carboxylateUniRule annotation

GO - Molecular functioni

  1. chlorophyll binding Source: UniProtKB-HAMAP
  2. electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Source: InterPro
  3. iron ion binding Source: UniProtKB-HAMAP
  4. oxygen evolving activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. photosynthetic electron transport in photosystem II Source: InterPro
  2. protein-chromophore linkage Source: UniProtKB-KW
  3. response to herbicide Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Herbicide resistance, Photosynthesis, Transport

Keywords - Ligandi

Calcium, Chlorophyll, Chromophore, Iron, Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:PSBA1-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem II protein D1 1UniRule annotation (EC:1.10.3.9UniRule annotation)
Short name:
PSII D1 protein 1UniRule annotation
Alternative name(s):
Photosystem II Q(B) protein 1UniRule annotation
Gene namesi
Name:psbA1UniRule annotationCurated
Synonyms:psbA-1
Ordered Locus Names:slr1181
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

Subcellular locationi

Cellular thylakoid membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei29 – 4618HelicalUniRule annotationAdd
BLAST
Transmembranei118 – 13316HelicalUniRule annotationAdd
BLAST
Transmembranei142 – 15615HelicalUniRule annotationAdd
BLAST
Transmembranei197 – 21822HelicalUniRule annotationAdd
BLAST
Transmembranei274 – 28815HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. photosystem II Source: UniProtKB-KW
  3. thylakoid membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Photosystem II, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Photosystem II protein D1 1UniRule annotationPRO_0000090492Add
BLAST
Propeptidei345 – 36016UniRule annotation1 PublicationPRO_0000316428Add
BLAST

Post-translational modificationi

C-terminally processed by CtpA; processing is essential to allow assembly of the oxygen-evolving complex and photosynthetic growth.UniRule annotation1 Publication
Tyr-161 forms a radical intermediate that is referred to as redox-active TyrZ, YZ or Y-Z.UniRule annotation
C-terminally processed by CtpA; processing is essential to allow assembly of the oxygen-evolving complex and thus photosynthetic growth.UniRule annotation

Proteomic databases

PaxDbiP07826.

Interactioni

Subunit structurei

Cyanobacterial PSII is composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins PsbO, PsbU, PsbV and a large number of cofactors. It forms dimeric complexes.UniRule annotation

Protein-protein interaction databases

IntActiP07826. 2 interactions.
STRINGi1148.slr1181.

Structurei

3D structure databases

ProteinModelPortaliP07826.
SMRiP07826. Positions 12-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni264 – 2652Quinone (B)UniRule annotation

Sequence similaritiesi

Belongs to the reaction center PufL/M/PsbA/D family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG04871.
HOGENOMiHOG000246913.
InParanoidiP07826.
KOiK02703.
OMAiHERNVHN.
OrthoDBiEOG6Q2SGP.
PhylomeDBiP07826.

Family and domain databases

Gene3Di1.20.85.10. 1 hit.
HAMAPiMF_01379. PSII_PsbA_D1.
InterProiIPR000484. Photo_RC_L/M.
IPR005867. PSII_D1.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01151. psbA. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07826-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTQLGLQE QSLWSRFCCW ITSTSNRLYI GWFGVLMIPT LLTATTCFII
60 70 80 90 100
AFIAAPPVDI DGIREPIAGS LLYGNNIITA AVVPSSNAIG LHFYPIWEAH
110 120 130 140 150
SLDEWLYNGG PYQLIVFHFL IGIFCYLGRQ WELSYRLGMR PWICVAYSAP
160 170 180 190 200
VAAATATLLI YSIGQGSFSD GLPLGISGTF NFMLVLQAEH NVLMHPFHML
210 220 230 240 250
GVAGVFGGAL FAAMHGSLVT SSLIRETTEV ESQNQGYKFG QEEETYNIVA
260 270 280 290 300
AHGYFGRLIF QYASFNNSRA LHFFLGAWPV VGIWFAALAV CCFAFNLNGF
310 320 330 340 350
NFNQSILDAQ GRPVSTWADV INRANIGFEV MHERNVHNFP LDLASGDAQM
360
VALNAPAIEG
Length:360
Mass (Da):39,651
Last modified:October 31, 1997 - v3
Checksum:i41A74A7819FE90D2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901G → A in CAA68778 (PubMed:3122184).Curated
Sequence conflicti115 – 1151I → N in CAA68778 (PubMed:3122184).Curated
Sequence conflicti273 – 2731F → S in CAA68778 (PubMed:3122184).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00885 Genomic DNA. Translation: CAA68778.1.
BA000022 Genomic DNA. Translation: BAA18829.1.
PIRiS76917. F2YB13.
RefSeqiNP_443017.1. NC_000911.1.
YP_005653076.1. NC_017277.1.
YP_007452892.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA18829; BAA18829; BAA18829.
KEGGisyn:slr1181.
PATRICi23843888. VBISynSp132158_3474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00885 Genomic DNA. Translation: CAA68778.1.
BA000022 Genomic DNA. Translation: BAA18829.1.
PIRiS76917. F2YB13.
RefSeqiNP_443017.1. NC_000911.1.
YP_005653076.1. NC_017277.1.
YP_007452892.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP07826.
SMRiP07826. Positions 12-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP07826. 2 interactions.
STRINGi1148.slr1181.

Proteomic databases

PaxDbiP07826.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18829; BAA18829; BAA18829.
KEGGisyn:slr1181.
PATRICi23843888. VBISynSp132158_3474.

Phylogenomic databases

eggNOGiNOG04871.
HOGENOMiHOG000246913.
InParanoidiP07826.
KOiK02703.
OMAiHERNVHN.
OrthoDBiEOG6Q2SGP.
PhylomeDBiP07826.

Enzyme and pathway databases

BioCyciMetaCyc:PSBA1-MONOMER.

Family and domain databases

Gene3Di1.20.85.10. 1 hit.
HAMAPiMF_01379. PSII_PsbA_D1.
InterProiIPR000484. Photo_RC_L/M.
IPR005867. PSII_D1.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01151. psbA. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a member of the psbA multigene family from the unicellular cyanobacterium Synechocystis 6803."
    Osiewacz H.D., McIntosh L.
    Nucleic Acids Res. 15:10585-10585(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  3. "Thylakoid protein phosphorylation in evolutionally divergent species with oxygenic photosynthesis."
    Pursiheimo S., Rintamaeki E., Baena-Gonzalez E., Aro E.-M.
    FEBS Lett. 423:178-182(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, LACK OF PHOSPHORYLATION.
    Strain: PCC 6803 / Kazusa.
  4. "Molecular cloning and characterization of the ctpA gene encoding a carboxyl-terminal processing protease. Analysis of a spontaneous photosystem II-deficient mutant strain of the cyanobacterium Synechocystis sp. PCC 6803."
    Shestakov S.V., Anbudurai P.R., Stanbekova G.E., Gadzhiev A., Lind L.K., Pakrasi H.B.
    J. Biol. Chem. 269:19354-19359(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROBABLE CLEAVAGE.
    Strain: ATCC 27184 / PCC 6803 / N-1.

Entry informationi

Entry nameiPSBA1_SYNY3
AccessioniPrimary (citable) accession number: P07826
Secondary accession number(s): P74710
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1988
Last sequence update: October 31, 1997
Last modified: March 31, 2015
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Cyanobacteria usually contain more than 2 copies of the psbA gene.UniRule annotation1 Publication
2 of the reaction center chlorophylls (ChlD1 and ChlD2) are entirely coordinated by water.UniRule annotation
Herbicides such as atrazine, BNT, diuron or ioxynil bind in the Q(B) binding site and block subsequent electron transfer.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.