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P07826

- PSBA1_SYNY3

UniProt

P07826 - PSBA1_SYNY3

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Protein

Photosystem II protein D1 1

Gene

psbA1

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Photosystem II (PSII) is a light-driven water plastoquinone oxidoreductase that uses light energy to abstract electrons from H2O, generating a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1/D2 (PsbA/PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors.By similarity

Catalytic activityi

2 H2O + 2 plastoquinone + 4 light = O2 + 2 plastoquinol.UniRule annotation

Cofactori

The D1/D2 heterodimer binds P680, chlorophylls that are the primary electron donor of PSII, and subsequent electron acceptors. It shares a non-heme iron and each subunit binds pheophytin, quinone, additional chlorophylls, carotenoids and lipids. D1 provides most of the ligands for the Mn4-Ca-O5 cluster of the oxygen-evolving complex (OEC). PSII binds additional chlorophylls, carotenoids and specific lipids.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei161 – 1611Tyrosine radical intermediateBy similarity
Metal bindingi170 – 1701Calcium-manganese-oxide cluster [Ca-4Mn-5O]; calciumUniRule annotation
Metal bindingi170 – 1701Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 1UniRule annotation
Metal bindingi189 – 1891Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2UniRule annotation
Metal bindingi215 – 2151Iron; shared with heterodimeric partnerUniRule annotation
Metal bindingi272 – 2721Iron; shared with heterodimeric partnerUniRule annotation
Metal bindingi332 – 3321Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2; via tele nitrogenUniRule annotation
Metal bindingi333 – 3331Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 1UniRule annotation
Metal bindingi333 – 3331Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 3UniRule annotation
Metal bindingi342 – 3421Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 2UniRule annotation
Metal bindingi342 – 3421Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 4UniRule annotation
Metal bindingi344 – 3441Calcium-manganese-oxide cluster [Ca-4Mn-5O]; calcium; via carboxylateUniRule annotation
Metal bindingi344 – 3441Calcium-manganese-oxide cluster [Ca-4Mn-5O]; manganese 4; via carboxylateUniRule annotation

GO - Molecular functioni

  1. electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Source: InterPro
  2. iron ion binding Source: UniProtKB-HAMAP
  3. oxidoreductase activity Source: UniProtKB-KW

GO - Biological processi

  1. photosynthetic electron transport in photosystem II Source: InterPro
  2. response to herbicide Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Herbicide resistance, Photosynthesis, Transport

Keywords - Ligandi

Calcium, Iron, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:PSBA1-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem II protein D1 1UniRule annotation (EC:1.10.3.9UniRule annotation)
Alternative name(s):
32 kDa thylakoid membrane protein 1UniRule annotation
Photosystem Q(B) protein 1UniRule annotation
Gene namesi
Name:psbA1UniRule annotation
Synonyms:psbA-1
Ordered Locus Names:slr1181
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cellular thylakoid membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. photosystem II Source: UniProtKB-KW
  3. thylakoid membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Photosystem II, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Photosystem II protein D1 1PRO_0000090492Add
BLAST
Propeptidei345 – 36016Cleavage; by CtpA1 PublicationUniRule annotationPRO_0000316428Add
BLAST

Post-translational modificationi

C-terminally processed by CtpA; processing is essential to allow assembly of the oxygen-evolving complex and photosynthetic growth.1 Publication
Tyr-161 forms a radical intermediate that is referred to as redox-active TyrZ, YZ or Y-Z.By similarity

Proteomic databases

PaxDbiP07826.

Interactioni

Subunit structurei

Cyanobacterial PSII is composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Ycf12, 3 peripheral proteins PsbO, PsbU, PsbV and a large number of cofactors. It forms dimeric complexes.By similarity

Protein-protein interaction databases

IntActiP07826. 2 interactions.
STRINGi1148.slr1181.

Structurei

3D structure databases

ProteinModelPortaliP07826.
SMRiP07826. Positions 12-344.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei36 – 5621HelicalUniRule annotationAdd
BLAST
Transmembranei109 – 12921HelicalUniRule annotationAdd
BLAST
Transmembranei141 – 16424HelicalUniRule annotationAdd
BLAST
Transmembranei192 – 21827HelicalUniRule annotationAdd
BLAST
Transmembranei269 – 28921HelicalUniRule annotationAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the reaction center PufL/M/PsbA/D family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG04871.
HOGENOMiHOG000246913.
InParanoidiP07826.
KOiK02703.
OMAiHERNVHN.
OrthoDBiEOG6Q2SGP.
PhylomeDBiP07826.

Family and domain databases

Gene3Di1.20.85.10. 1 hit.
HAMAPiMF_01379. PSII_PsbA_D1.
InterProiIPR000484. Photo_RC_L/M.
IPR005867. PSII_D1.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01151. psbA. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07826-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTTQLGLQE QSLWSRFCCW ITSTSNRLYI GWFGVLMIPT LLTATTCFII
60 70 80 90 100
AFIAAPPVDI DGIREPIAGS LLYGNNIITA AVVPSSNAIG LHFYPIWEAH
110 120 130 140 150
SLDEWLYNGG PYQLIVFHFL IGIFCYLGRQ WELSYRLGMR PWICVAYSAP
160 170 180 190 200
VAAATATLLI YSIGQGSFSD GLPLGISGTF NFMLVLQAEH NVLMHPFHML
210 220 230 240 250
GVAGVFGGAL FAAMHGSLVT SSLIRETTEV ESQNQGYKFG QEEETYNIVA
260 270 280 290 300
AHGYFGRLIF QYASFNNSRA LHFFLGAWPV VGIWFAALAV CCFAFNLNGF
310 320 330 340 350
NFNQSILDAQ GRPVSTWADV INRANIGFEV MHERNVHNFP LDLASGDAQM
360
VALNAPAIEG
Length:360
Mass (Da):39,651
Last modified:November 1, 1997 - v3
Checksum:i41A74A7819FE90D2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901G → A in CAA68778. (PubMed:3122184)Curated
Sequence conflicti115 – 1151I → N in CAA68778. (PubMed:3122184)Curated
Sequence conflicti273 – 2731F → S in CAA68778. (PubMed:3122184)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00885 Genomic DNA. Translation: CAA68778.1.
BA000022 Genomic DNA. Translation: BAA18829.1.
PIRiS76917. F2YB13.
RefSeqiNP_443017.1. NC_000911.1.
YP_005653076.1. NC_017277.1.
YP_007452892.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA18829; BAA18829; BAA18829.
GeneIDi951901.
KEGGisyn:slr1181.
syy:SYNGTS_3123.
syz:MYO_131590.
PATRICi23843888. VBISynSp132158_3474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00885 Genomic DNA. Translation: CAA68778.1 .
BA000022 Genomic DNA. Translation: BAA18829.1 .
PIRi S76917. F2YB13.
RefSeqi NP_443017.1. NC_000911.1.
YP_005653076.1. NC_017277.1.
YP_007452892.1. NC_020286.1.

3D structure databases

ProteinModelPortali P07826.
SMRi P07826. Positions 12-344.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P07826. 2 interactions.
STRINGi 1148.slr1181.

Proteomic databases

PaxDbi P07826.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA18829 ; BAA18829 ; BAA18829 .
GeneIDi 951901.
KEGGi syn:slr1181.
syy:SYNGTS_3123.
syz:MYO_131590.
PATRICi 23843888. VBISynSp132158_3474.

Phylogenomic databases

eggNOGi NOG04871.
HOGENOMi HOG000246913.
InParanoidi P07826.
KOi K02703.
OMAi HERNVHN.
OrthoDBi EOG6Q2SGP.
PhylomeDBi P07826.

Enzyme and pathway databases

BioCyci MetaCyc:PSBA1-MONOMER.

Family and domain databases

Gene3Di 1.20.85.10. 1 hit.
HAMAPi MF_01379. PSII_PsbA_D1.
InterProi IPR000484. Photo_RC_L/M.
IPR005867. PSII_D1.
[Graphical view ]
Pfami PF00124. Photo_RC. 1 hit.
[Graphical view ]
PRINTSi PR00256. REACTNCENTRE.
SUPFAMi SSF81483. SSF81483. 1 hit.
TIGRFAMsi TIGR01151. psbA. 1 hit.
PROSITEi PS00244. REACTION_CENTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a member of the psbA multigene family from the unicellular cyanobacterium Synechocystis 6803."
    Osiewacz H.D., McIntosh L.
    Nucleic Acids Res. 15:10585-10585(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  3. "Thylakoid protein phosphorylation in evolutionally divergent species with oxygenic photosynthesis."
    Pursiheimo S., Rintamaeki E., Baena-Gonzalez E., Aro E.-M.
    FEBS Lett. 423:178-182(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, LACK OF PHOSPHORYLATION.
    Strain: PCC 6803 / Kazusa.
  4. "Molecular cloning and characterization of the ctpA gene encoding a carboxyl-terminal processing protease. Analysis of a spontaneous photosystem II-deficient mutant strain of the cyanobacterium Synechocystis sp. PCC 6803."
    Shestakov S.V., Anbudurai P.R., Stanbekova G.E., Gadzhiev A., Lind L.K., Pakrasi H.B.
    J. Biol. Chem. 269:19354-19359(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROBABLE CLEAVAGE.
    Strain: ATCC 27184 / PCC 6803 / N-1.

Entry informationi

Entry nameiPSBA1_SYNY3
AccessioniPrimary (citable) accession number: P07826
Secondary accession number(s): P74710
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Herbicides such as atrazine, BNT, diuron or ioxynil bind to Q(B) and block electron transport.UniRule annotation
Cyanobacteria usually contain more than 2 copies of the psbA gene.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3