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Reviewed, UniProtKB/Swiss-Prot P07825 (SYPH_RAT)

Last modified November 3, 2009. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Synaptophysin
Alternative name(s):
    Major synaptic vesicle protein p38
Gene names
Name: Syp
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity By similarity.

Subunit structure

Homohexamer or homotetramer.

Subcellular location

Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Multi-pass membrane protein. Cell junction › synapse › synaptosome.

Tissue specificity

Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

Domain

The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.

Post-translational modification

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. Ref.7

Sequence similarities

Belongs to the synaptophysin/synaptobrevin family.

Contains 1 MARVEL domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307Synaptophysin
PRO_0000179163

Regions

Topological domain1 – 1919Cytoplasmic Potential
Transmembrane20 – 4324 Potential
Topological domain44 – 10158Vesicular Potential
Transmembrane102 – 12524 Potential
Topological domain126 – 1327Cytoplasmic Potential
Transmembrane133 – 15624 Potential
Topological domain157 – 19438Vesicular Potential
Transmembrane195 – 21824 Potential
Topological domain219 – 30789Cytoplasmic Potential
Domain15 – 222208MARVEL
Region249 – 29850Repeats, Gly-rich

Amino acid modifications

Modified residue751Phosphotyrosine By similarity
Modified residue2211Phosphothreonine Potential
Modified residue2731Phosphotyrosine Potential
Modified residue2891Phosphotyrosine Potential
Glycosylation531N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
P07825-1 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: A245E4F70BF0EAF0

FASTA30733,311
        10         20         30         40         50         60 
MDVVNQLVAG GQFRVVKEPL GFVKVLQWVF AIFAFATCGS YTGELRLSVE CANKTESALN 

        70         80         90        100        110        120 
IEVEFEYPFR LHQVYFDAPS CVKGGTTKIF LVGDYSSSAE FFVTVAVFAF LYSMGALATY 

       130        140        150        160        170        180 
IFLQNKYREN NKGPMMDFLA TAVFAFMWLV SSSAWAKGLS DVKMATDPEN IIKEMPMCRQ 

       190        200        210        220        230        240 
TGNTCKELRD PVTSGLNTSV VFGFLNLVLW VGNLWFVFKE TGWAAPFMRA PPGAPEKQPA 

       250        260        270        280        290        300 
PGDAYGDAGY GQGPGGYGPQ DSYGPQGGYQ PDYGQPASGG GGYGPQGDYG QQGYGQQGAP 


TSFSNQM

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References

« Hide 'large scale' references
[1]"Synaptophysin: molecular organization and mRNA expression as determined from cloned cDNA."
Leube R.E., Kaiser P., Seiter A., Zimbelmann R., Franke W.W., Rehm H., Knaus P., Prior P., Betz H., Reinke H., Beyreuther K., Wiedenmann B.
EMBO J. 6:3261-3268(1987) [PubMed: 3123215] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The cDNA and derived amino acid sequences for rat and human synaptophysin."
Suedhof T.C., Lottspeich F., Greengard P., Mehl E., Jahn R.
Nucleic Acids Res. 15:9607-9607(1987) [PubMed: 3120152] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"A synaptic vesicle protein with a novel cytoplasmic domain and four transmembrane regions."
Suedhof T.C., Lottspeich F., Greengard P., Mehl E., Jahn R.
Science 238:1142-1144(1987) [PubMed: 3120313] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[4]"The synaptophysin-encoding gene in rat and man is specifically transcribed in neuroendocrine cells."
Bargou R.C.E.F., Leube R.E.
Gene 99:197-204(1991) [PubMed: 1902431] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[6]"Cloning and sequence analysis of cDNA encoding p38, a major synaptic vesicle protein."
Buckley K.M., Floor E., Kelly R.B.
J. Cell Biol. 105:2447-2456(1987) [PubMed: 3121632] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-307.
Tissue: Brain.
[7]"Regulation of synaptophysin degradation by mammalian homologues of Seven in Absentia."
Wheeler T.C., Chin L.-S., Li Y., Roudabush F.L., Li L.
J. Biol. Chem. 277:10273-10282(2002) [PubMed: 11786535] [Abstract]
Cited for: INTERACTION WITH SIAH1 AND SIAH2, DEGRADATION.
Strain: Sprague-Dawley.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X06177 mRNA. Translation: CAA29543.1.
X06388 mRNA. Translation: CAA29685.1.
M58396 expand/collapse EMBL AC list , M58402, M58401, M58399 Genomic DNA. Translation: AAA42196.1.
BC099798 mRNA. Translation: AAH99798.1.
X06655 mRNA. Translation: CAA29854.1.
IPIIPI00211360.
PIRB27287. JU0464.
RefSeqNP_036796.1.
UniGeneRn.11067

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP07825. 5 interactions.
STRINGP07825.

PTM databases

PhosphoSiteP07825.

Proteomic databases

PRIDEP07825.

Genome annotation databases

EnsemblENSRNOT00000013724; ENSRNOP00000013724; ENSRNOG00000010223; Rattus norvegicus. [Genome view]
GeneID24804.
KEGGrno:24804.
UCSCNM_012664. rat.

Organism-specific databases

CTD24804.
RGD3802. Syp.

Phylogenomic databases

HOVERGENP07825.

Gene expression databases

ArrayExpressP07825.
GenevestigatorP07825.
GermOnlineENSRNOG00000010223. Rattus norvegicus.

Family and domain databases

InterProIPR008253. Marvel.
IPR001285. Synaptophysin/porin.
IPR015457. Synaptophysin_.
[Graphical view]
PANTHERPTHR10306. Synaptophysin. 1 hit.
PTHR10306:SF2. Synaptophysin_. 1 hit.
PfamPF01284. MARVEL. 1 hit.
[Graphical view]
PRINTSPR00220. SYNAPTOPHYSN.
PROSITEPS51225. MARVEL. 1 hit.
PS00604. SYNAPTOP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio604472.

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Entry information

Entry nameSYPH_RAT
AccessionPrimary (citable) accession number: P07825
Secondary accession number(s): Q499R3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 3, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents