Iron(3+)-hydroxamate-binding protein fhuD precursor

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Reviewed, UniProtKB/Swiss-Prot P07822 (FHUD_ECOLI)

Last modified June 16, 2009. Version 99. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Iron(3+)-hydroxamate-binding protein fhuD
Alternative name(s):
    Iron(III)-hydroxamate-binding protein fhuD
    Ferrichrome-binding periplasmic protein
    Ferric hydroxamate uptake protein D

Gene names

Name:	fhuD
Ordered Locus Names:	b0152, JW0148

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Part of the ABC transporter complex fhuCDB involved in iron³⁺-hydroxamate import. Binds the iron³⁺-hydroxamate complex and transfers it to the membrane-bound permease. Required for the transport of all iron³⁺-hydroxamate siderophores such as ferrichrome, gallichrome, desferrioxamine, coprogen, aerobactin, shizokinen, rhodotorulic acid and the antibiotic albomycin.
Subunit structure	The complex is composed of an ATP-binding protein (fhuC), two transmembrane proteins (fhuB) and a solute-binding protein (fhuD).
Subcellular location	Periplasm.
Sequence similarities	Belongs to the bacterial solute-binding protein 8 family. Contains 1 Fe/B12 periplasmic-binding domain.

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Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Cellular component	Periplasm
Domain	Signal
Ligand	Iron
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	high-affinity iron ion transport Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 30

Chain

31 – 296

266

Iron(3+)-hydroxamate-binding protein fhuD

PRO_0000031824

Regions

Domain

37 – 296

260

Fe/B12 periplasmic-binding

Experimental info

Sequence conflict

L → V in CAA29255. Ref.1

Sequence conflict

154

H → D Ref.2

Sequence conflict

154

H → D Ref.3

Secondary structure

296

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P07822-1 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 4687A34771397D9F
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FASTA

296

32,998

        10         20         30         40         50         60 
MSGLPLISRR RLLTAMALSP LLWQMNTAHA AAIDPNRIVA LEWLPVELLL ALGIVPYGVA 

        70         80         90        100        110        120 
DTINYRLWVS EPPLPDSVID VGLRTEPNLE LLTEMKPSFM VWSAGYGPSP EMLARIAPGR 

       130        140        150        160        170        180 
GFNFSDGKQP LAMARKSLTE MADLLNLQSA AETHLAQYED FIRSMKPRFV KRGARPLLLT 

       190        200        210        220        230        240 
TLIDPRHMLV FGPNSLFQEI LDEYGIPNAW QGETNFWGST AVSIDRLAAY KDVDVLCFDH 

       250        260        270        280        290 
DNSKDMDALM ATPLWQAMPF VRAGRFQRVP AVWFYGATLS AMHFVRVLDN AIGGKA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"fhuC and fhuD genes for iron (III)-ferrichrome transport into Escherichia coli K-12." Coulton J.W., Mason P., Allatt D.D. J. Bacteriol. 169:3844-3849(1987) [PubMed: 3301821] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Nucleotide sequence of the fhuC and fhuD genes involved in iron (III) hydroxamate transport: domains in FhuC homologous to ATP-binding proteins." Burkhardt R., Braun V. Mol. Gen. Genet. 209:49-55(1987) [PubMed: 2823072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 154. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"In vivo reconstitution of an active siderophore transport system by a binding protein derivative lacking a signal sequence." Rohrback M.R., Paul S., Koster W. Mol. Gen. Genet. 248:33-42(1995) [PubMed: 7651325] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-39.
[8]	"The structure of the ferric siderophore binding protein FhuD complexed with gallichrome." Clarke T.E., Ku S.-Y., Dougan D.R., Vogel H.J., Tari L.W. Nat. Struct. Biol. 7:287-291(2000) [PubMed: 10742172] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 31-296 IN COMPLEX WITH GALLICHROME.
[9]	"X-ray crystallographic structures of the Escherichia coli periplasmic protein FhuD bound to hydroxamate-type siderophores and the antibiotic albomycin." Clarke T.E., Braun V., Winkelmann G., Tari L.W., Vogel H.J. J. Biol. Chem. 277:13966-13972(2002) [PubMed: 11805094] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 31-296 IN COMPLEX WITH COPROGEN; DESFERRIOXAMINE AND ALBOMYCIN.

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Cross-references

Sequence databases

X05810 Genomic DNA. Translation: CAA29255.1.
M12486 Genomic DNA. Translation: AAB61770.1.
U70214 Genomic DNA. Translation: AAB08582.1.
U00096 Genomic DNA. Translation: AAC73263.1.
AP009048 Genomic DNA. Translation: BAB96728.2.

PIR

QRECFD. H64738.

RefSeq

AP_000813.1.
NP_414694.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EFD	X-ray	1.90	N	33-296	[»]
1ESZ	X-ray	2.00	A	31-296	[»]
1K2V	X-ray	1.97	N	31-296	[»]
1K7S	X-ray	2.60	N	33-296	[»]

ModBase

Search...

Protein family/group databases

TCDB

3.A.1.14.3. ATP-binding cassette (ABC) superfamily.

Genome annotation databases

GeneID

947510.

GenomeReviews

Gene locus JW0148 in contig AP009048_GR.
Gene locus b0152 in contig U00096_GR.

KEGG

ecj:JW0148.
eco:b0152.

Organism-specific databases

EchoBASE

EB0301.

EcoGene

EG10305. fhuD.

CMR

Search...

Phylogenomic databases

HOGENOM

P07822.

OMA

P07822. LPAIWTF.

Enzyme and pathway databases

BioCyc

EcoCyc:FHUD-MON.

Family and domain databases

InterPro

IPR008091. Ferrichrome_bd.
IPR002491. Periplasmic_BP.
[Graphical view]

Pfam

PF01497. Peripla_BP_2. 1 hit.
[Graphical view]

PRINTS

PR01715. FERRIBNDNGPP.

PROSITE

PS50983. FE_B12_PBP. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

FHUD_ECOLI

Accession

Primary (citable) accession number: P07822
Secondary accession number(s): P77711

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 99 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families