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Reviewed, UniProtKB/Swiss-Prot P07822 (FHUD_ECOLI)

Last modified June 16, 2009. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Iron(3+)-hydroxamate-binding protein fhuD
Alternative name(s):
    Iron(III)-hydroxamate-binding protein fhuD
    Ferrichrome-binding periplasmic protein
    Ferric hydroxamate uptake protein D
Gene names
Name: fhuD
Ordered Locus Names: b0152, JW0148
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Part of the ABC transporter complex fhuCDB involved in iron3+-hydroxamate import. Binds the iron3+-hydroxamate complex and transfers it to the membrane-bound permease. Required for the transport of all iron3+-hydroxamate siderophores such as ferrichrome, gallichrome, desferrioxamine, coprogen, aerobactin, shizokinen, rhodotorulic acid and the antibiotic albomycin.

Subunit structure

The complex is composed of an ATP-binding protein (fhuC), two transmembrane proteins (fhuB) and a solute-binding protein (fhuD).

Subcellular location

Periplasm.

Sequence similarities

Belongs to the bacterial solute-binding protein 8 family.

Contains 1 Fe/B12 periplasmic-binding domain.

Ontologies

Keywords
   Biological processIon transport
Iron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandIron
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processhigh-affinity iron ion transport

Inferred from electronic annotation. Source: InterPro

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioniron ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

iron ion transmembrane transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030
Chain31 – 296266Iron(3+)-hydroxamate-binding protein fhuD
PRO_0000031824

Regions

Domain37 – 296260Fe/B12 periplasmic-binding

Experimental info

Sequence conflict481L → V in CAA29255. Ref.1
Sequence conflict1541H → D Ref.2
Sequence conflict1541H → D Ref.3

Secondary structure

.................................................. 296
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07822-1 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 4687A34771397D9F

FASTA29632,998
        10         20         30         40         50         60 
MSGLPLISRR RLLTAMALSP LLWQMNTAHA AAIDPNRIVA LEWLPVELLL ALGIVPYGVA 

        70         80         90        100        110        120 
DTINYRLWVS EPPLPDSVID VGLRTEPNLE LLTEMKPSFM VWSAGYGPSP EMLARIAPGR 

       130        140        150        160        170        180 
GFNFSDGKQP LAMARKSLTE MADLLNLQSA AETHLAQYED FIRSMKPRFV KRGARPLLLT 

       190        200        210        220        230        240 
TLIDPRHMLV FGPNSLFQEI LDEYGIPNAW QGETNFWGST AVSIDRLAAY KDVDVLCFDH 

       250        260        270        280        290 
DNSKDMDALM ATPLWQAMPF VRAGRFQRVP AVWFYGATLS AMHFVRVLDN AIGGKA 

« Hide

References

« Hide 'large scale' references
[1]"fhuC and fhuD genes for iron (III)-ferrichrome transport into Escherichia coli K-12."
Coulton J.W., Mason P., Allatt D.D.
J. Bacteriol. 169:3844-3849(1987) [PubMed: 3301821] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Nucleotide sequence of the fhuC and fhuD genes involved in iron (III) hydroxamate transport: domains in FhuC homologous to ATP-binding proteins."
Burkhardt R., Braun V.
Mol. Gen. Genet. 209:49-55(1987) [PubMed: 2823072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
Fujita N., Mori H., Yura T., Ishihama A.
Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[6]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 154.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]"In vivo reconstitution of an active siderophore transport system by a binding protein derivative lacking a signal sequence."
Rohrback M.R., Paul S., Koster W.
Mol. Gen. Genet. 248:33-42(1995) [PubMed: 7651325] [Abstract]
Cited for: PROTEIN SEQUENCE OF 32-39.
[8]"The structure of the ferric siderophore binding protein FhuD complexed with gallichrome."
Clarke T.E., Ku S.-Y., Dougan D.R., Vogel H.J., Tari L.W.
Nat. Struct. Biol. 7:287-291(2000) [PubMed: 10742172] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 31-296 IN COMPLEX WITH GALLICHROME.
[9]"X-ray crystallographic structures of the Escherichia coli periplasmic protein FhuD bound to hydroxamate-type siderophores and the antibiotic albomycin."
Clarke T.E., Braun V., Winkelmann G., Tari L.W., Vogel H.J.
J. Biol. Chem. 277:13966-13972(2002) [PubMed: 11805094] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 31-296 IN COMPLEX WITH COPROGEN; DESFERRIOXAMINE AND ALBOMYCIN.

Cross-references

Sequence databases

X05810 Genomic DNA. Translation: CAA29255.1.
M12486 Genomic DNA. Translation: AAB61770.1.
U70214 Genomic DNA. Translation: AAB08582.1.
U00096 Genomic DNA. Translation: AAC73263.1.
AP009048 Genomic DNA. Translation: BAB96728.2.
PIRQRECFD. H64738.
RefSeqAP_000813.1.
NP_414694.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1EFDX-ray1.90N33-296[»]
1ESZX-ray2.00A31-296[»]
1K2VX-ray1.97N31-296[»]
1K7SX-ray2.60N33-296[»]
ModBaseSearch...

Protein family/group databases

TCDB3.A.1.14.3. ATP-binding cassette (ABC) superfamily.

Genome annotation databases

GeneID947510.
GenomeReviewsGene locus JW0148 in contig AP009048_GR.
Gene locus b0152 in contig U00096_GR.
KEGGecj:JW0148.
eco:b0152.

Organism-specific databases

EchoBASEEB0301.
EcoGeneEG10305. fhuD.
CMRSearch...

Phylogenomic databases

HOGENOMP07822.
OMAP07822. LPAIWTF.

Enzyme and pathway databases

BioCycEcoCyc:FHUD-MON.

Family and domain databases

InterProIPR008091. Ferrichrome_bd.
IPR002491. Periplasmic_BP.
[Graphical view]
PfamPF01497. Peripla_BP_2. 1 hit.
[Graphical view]
PRINTSPR01715. FERRIBNDNGPP.
PROSITEPS50983. FE_B12_PBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFHUD_ECOLI
AccessionPrimary (citable) accession number: P07822
Secondary accession number(s): P77711
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1997
Last modified: June 16, 2009
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents