P07821 (FHUC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Iron(3+)-hydroxamate import ATP-binding protein FhuC
EC=3.6.3.34

Alternative name(s):
Ferric hydroxamate uptake protein C
Ferrichrome transport ATP-binding protein FhuC
Iron(III)-hydroxamate import ATP-binding protein FhuC

Gene names

Name:	fhuC
Ordered Locus Names:	b0151, JW0147

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex FhuCDB involved in iron3+-hydroxamate import. Responsible for energy coupling to the transport system. Ref.7
Catalytic activity	ATP + H2O + iron chelate(Out) = ADP + phosphate + iron chelate(In).
Subunit structure	The complex is composed of an ATP-binding protein (FhuC), two transmembrane proteins (FhuB) and a solute-binding protein (FhuD).
Subcellular location	Cell inner membrane; Peripheral membrane protein Ref.7.
Sequence similarities	Belongs to the ABC transporter superfamily. Iron (Fe3+)-hydroxamate importer (TC 3.A.1.14.7) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Iron Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW iron-chelate-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Iron(3+)-hydroxamate import ATP-binding protein FhuC		PRO_0000092327
Regions
	Domain	12 – 248	237	ABC transporter
	Nucleotide binding	44 – 51	8	ATP Potential
	Nucleotide binding	168 – 179	12	ATP Potential
Experimental info
	Sequence conflict	31	1	S → C in CAA29254. Ref.2
	Sequence conflict	226 – 249	24	MIAQG…YGIPM → SDCSGNACGNYARRNPRNDL WHPD in AAB61769. Ref.1
	Sequence conflict	226 – 249	24	MIAQG…YGIPM → SDCSGNACGNYARRNPRNDL WHPD no nucleotide entry Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P07821 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 2511770D6D9EE772	FASTA	265	28,886
	10 20 30 40 50 60 MQEYTNHSDT TFALRNISFR VPGRTLLHPL SLTFPAGKVT GLIGHNGSGK STLLKMLGRH 70 80 90 100 110 120 QPPSEGEILL DAQPLESWSS KAFARKVAYL PQQLPPAEGM TVRELVAIGR YPWHGALGRF 130 140 150 160 170 180 GAADREKVEE AISLVGLKPL AHRLVDSLSG GERQRAWIAM LVAQDSRCLL LDEPTSALDI 190 200 210 220 230 240 AHQVDVLSLV HRLSQERGLT VIAVLHDINM AARYCDYLVA LRGGEMIAQG TPAEIMRGET 250 260 LEMIYGIPMG ILPHPAGAAP VSFVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"fhuC and fhuD genes for iron (III)-ferrichrome transport into Escherichia coli K-12." Coulton J.W., Mason P., Allatt D.D. J. Bacteriol. 169:3844-3849(1987) [PubMed: 3301821] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Nucleotide sequence of the fhuC and fhuD genes involved in iron (III) hydroxamate transport: domains in FhuC homologous to ATP-binding proteins." Burkhardt R., Braun V. Mol. Gen. Genet. 209:49-55(1987) [PubMed: 2823072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Iron(III) hydroxamate transport in Escherichia coli K-12: FhuB-mediated membrane association of the FhuC protein and negative complementation of fhuC mutants." Schultz-Hauser G., Koester W., Schwarz H., Braun V. J. Bacteriol. 174:2305-2311(1992) [PubMed: 1551849] [Abstract] Cited for: FUNCTION IN IRON(3+)-HYDROXAMATE TRANSPORT, INTERACTION WITH FHUB, SUBCELLULAR LOCATION. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12486 Genomic DNA. Translation: AAB61769.1. X05810 Genomic DNA. Translation: CAA29254.1. U70214 Genomic DNA. Translation: AAB08581.1. U00096 Genomic DNA. Translation: AAC73262.1. AP009048 Genomic DNA. Translation: BAB96727.1.
PIR	QRECFH. G64738.
RefSeq	NP_414693.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P07821.
SMR	P07821. Positions 11-239.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07821. 3 interactions.
Protein family/group databases
TCDB	3.A.1.14.3. ATP-binding cassette (ABC) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003399; EBESCP00000003399; EBESCG00000002785. EBESCT00000016122; EBESCP00000015413; EBESCG00000015182.
GeneID	945250.
GenomeReviews	Gene locus JW0147 in contig AP009048_GR. Gene locus b0151 in contig U00096_GR.
KEGG	ecj:JW0147. eco:b0151.
PATRIC	32115411. VBIEscCol129921_0157.
Organism-specific databases
EchoBASE	EB0300.
EcoGene	EG10304. fhuC.
Phylogenomic databases
eggNOG	COG1120.
GeneTree	EBGT00050000008237.
HOGENOM	HBG758042.
OMA	NSKAFAR.
PhylomeDB	P07821.
ProtClustDB	PRK10575.
Enzyme and pathway databases
BioCyc	EcoCyc:FHUC-MONOMER.
Gene expression databases
Genevestigator	P07821.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR003593. ATPase_AAA+_core. [Graphical view]
KO	K02013.
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FHUC_ECOLI

Accession

Primary (citable) accession number: P07821
Secondary accession number(s): P77548

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program