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Protein

Iron(3+)-hydroxamate import ATP-binding protein FhuC

Gene

fhuC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex FhuCDB involved in iron3+-hydroxamate import. Responsible for energy coupling to the transport system.1 Publication

Catalytic activityi

ATP + H2O + iron chelate(Out) = ADP + phosphate + iron chelate(In).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 51ATPPROSITE-ProRule annotation8
Nucleotide bindingi168 – 179ATPPROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

ATP-binding, Iron, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:FHUC-MONOMER.
ECOL316407:JW0147-MONOMER.
MetaCyc:FHUC-MONOMER.

Protein family/group databases

TCDBi3.A.1.14.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron(3+)-hydroxamate import ATP-binding protein FhuCCurated (EC:3.6.3.341 Publication)
Alternative name(s):
Ferric hydroxamate uptake protein CCurated
Ferrichrome transport ATP-binding protein FhuCCurated
Iron(III)-hydroxamate import ATP-binding protein FhuCCurated
Gene namesi
Name:fhuC
Ordered Locus Names:b0151, JW0147
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10304. fhuC.

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: FhuB mediates the association of FhuC with the cytoplasmic membrane.1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi50K → Q: Lack of activity. 1 Publication1
Mutagenesisi172D → E: Lack of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000923271 – 265Iron(3+)-hydroxamate import ATP-binding protein FhuCAdd BLAST265

Proteomic databases

PaxDbiP07821.
PRIDEiP07821.

Expressioni

Inductioni

Induced 1.6-fold by hydroxyurea.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (FhuC), two transmembrane proteins (FhuB) and a solute-binding protein (FhuD) (Probable). FhuC interacts with FhuB (PubMed:1551849).Curated1 Publication

Protein-protein interaction databases

BioGridi4261072. 351 interactors.
IntActiP07821. 5 interactors.
STRINGi511145.b0151.

Structurei

3D structure databases

ProteinModelPortaliP07821.
SMRiP07821.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 248ABC transporterPROSITE-ProRule annotationAdd BLAST237

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105E0A. Bacteria.
COG1120. LUCA.
InParanoidiP07821.
KOiK02013.
OMAiMLGRHQK.
PhylomeDBiP07821.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEYTNHSDT TFALRNISFR VPGRTLLHPL SLTFPAGKVT GLIGHNGSGK
60 70 80 90 100
STLLKMLGRH QPPSEGEILL DAQPLESWSS KAFARKVAYL PQQLPPAEGM
110 120 130 140 150
TVRELVAIGR YPWHGALGRF GAADREKVEE AISLVGLKPL AHRLVDSLSG
160 170 180 190 200
GERQRAWIAM LVAQDSRCLL LDEPTSALDI AHQVDVLSLV HRLSQERGLT
210 220 230 240 250
VIAVLHDINM AARYCDYLVA LRGGEMIAQG TPAEIMRGET LEMIYGIPMG
260
ILPHPAGAAP VSFVY
Length:265
Mass (Da):28,886
Last modified:November 1, 1997 - v3
Checksum:i2511770D6D9EE772
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31S → C in CAA29254 (PubMed:2823072).Curated1
Sequence conflicti226 – 249MIAQG…YGIPM → SDCSGNACGNYARRNPRNDL WHPD in AAB61769 (PubMed:3301821).CuratedAdd BLAST24
Sequence conflicti226 – 249MIAQG…YGIPM → SDCSGNACGNYARRNPRNDL WHPD no nucleotide entry (PubMed:8202364).CuratedAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12486 Genomic DNA. Translation: AAB61769.1.
X05810 Genomic DNA. Translation: CAA29254.1.
U70214 Genomic DNA. Translation: AAB08581.1.
U00096 Genomic DNA. Translation: AAC73262.1.
AP009048 Genomic DNA. Translation: BAB96727.1.
PIRiG64738. QRECFH.
RefSeqiNP_414693.1. NC_000913.3.
WP_001158931.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73262; AAC73262; b0151.
BAB96727; BAB96727; BAB96727.
GeneIDi945250.
KEGGiecj:JW0147.
eco:b0151.
PATRICi32115411. VBIEscCol129921_0157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12486 Genomic DNA. Translation: AAB61769.1.
X05810 Genomic DNA. Translation: CAA29254.1.
U70214 Genomic DNA. Translation: AAB08581.1.
U00096 Genomic DNA. Translation: AAC73262.1.
AP009048 Genomic DNA. Translation: BAB96727.1.
PIRiG64738. QRECFH.
RefSeqiNP_414693.1. NC_000913.3.
WP_001158931.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP07821.
SMRiP07821.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261072. 351 interactors.
IntActiP07821. 5 interactors.
STRINGi511145.b0151.

Protein family/group databases

TCDBi3.A.1.14.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP07821.
PRIDEiP07821.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73262; AAC73262; b0151.
BAB96727; BAB96727; BAB96727.
GeneIDi945250.
KEGGiecj:JW0147.
eco:b0151.
PATRICi32115411. VBIEscCol129921_0157.

Organism-specific databases

EchoBASEiEB0300.
EcoGeneiEG10304. fhuC.

Phylogenomic databases

eggNOGiENOG4105E0A. Bacteria.
COG1120. LUCA.
InParanoidiP07821.
KOiK02013.
OMAiMLGRHQK.
PhylomeDBiP07821.

Enzyme and pathway databases

BioCyciEcoCyc:FHUC-MONOMER.
ECOL316407:JW0147-MONOMER.
MetaCyc:FHUC-MONOMER.

Miscellaneous databases

PROiP07821.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFHUC_ECOLI
AccessioniPrimary (citable) accession number: P07821
Secondary accession number(s): P77548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 150 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.