Reviewed, UniProtKB/Swiss-Prot P07819 (SCRB_BACSU)

Names and origin

Protein names

Recommended name:
    Sucrose-6-phosphate hydrolase
      Short name=Sucrase
    EC=3.2.1.26
Alternative name(s):
    Invertase

Gene names

Name:	sacA
Ordered Locus Names:	BSU38040
ORF Names:	ipa-50d

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-fructofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Sucrose-6-phosphate hydrolase		PRO_0000169870
Regions
	Region	40 – 43	4	Substrate binding By similarity
	Region	102 – 103	2	Substrate binding By similarity
	Region	161 – 162	2	Substrate binding By similarity
Sites
	Active site	43	1	By similarity
	Binding site	59	1	Substrate By similarity
	Binding site	220	1	Substrate By similarity
Experimental info
	Sequence conflict	155 – 156	2	GY → AIL in AAA22723. Ref.1
	Sequence conflict	155 – 156	2	GY → AIL in CAA51606. Ref.2
	Sequence conflict	161 – 164	4	RDPK → SRSE in AAA22723. Ref.1
	Sequence conflict	161 – 164	4	RDPK → SRSE in CAA51606. Ref.2
	Sequence conflict	448	1	L → F in AAA22723. Ref.1
	Sequence conflict	448	1	L → F in CAA51606. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P07819-1 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: 53D882B4772CB3CF	FASTA	479	54,814
	10 20 30 40 50 60 MTAHDQELRR RAYEEVEKKE PIANSDPHRQ HFHIMPPVGL LNDPNGVIYW KGSYHVFFQW 70 80 90 100 110 120 QPFQTGHGAK FWGHYTTQDV VNWKREEIAL APSDWFDKNG CYSGSAVTKD DRLYLFYTGN 130 140 150 160 170 180 VRDQDGNRET YQCLAVSDDG LSFEKKGVVA RLPEGYTAHF RDPKVWEHEG TWYMVIGAQT 190 200 210 220 230 240 ENLKGQAVLF ASDNLTEWRF LGPITGAGFN GLDDFGYMWE CPDLFSLQGS DVLIVSPQGL 250 260 270 280 290 300 EADGFRYQNV YQSGYFVGRL DYNKPELKHG EFTELDQGFD FYAPQTLEDD QGRRILFAWM 310 320 330 340 350 360 AVPDQDEGSH PTIDCHWIHC MTLPRQLTLS GQKLIQQPLP ELKAMRRNEK KIHINMHGSS 370 380 390 400 410 420 GALPVEKPER TEILLEDIHT ESGFSISIRG TATFSFHKDE GIVTLERKSF DGKRTEARHC 430 440 450 460 470 RIKDLHTVHM FLDASSVEIF INNGEEVLSA RYFPFPGNHE VTASATGKSE MNVGIWTLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the sucrase gene of Bacillus subtilis." Fouet A., Klier A., Rapoport G. Gene 45:221-225(1986) [PubMed: 3100393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325 degrees to 333 degrees." Glaser P., Kunst F., Arnaud M., Coudart M.P., Gonzales W., Hullo M.-F., Ionescu M., Lubochinsky B., Marcelino L., Moszer I., Presecan E., Santana M., Schneider E., Schweizer J., Vertes A., Rapoport G., Danchin A. Mol. Microbiol. 10:371-384(1993) [PubMed: 7934828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 155-156; 161-164 AND 448.
[5]	"Bacillus subtilis sucrose-specific enzyme II of the phosphotransferase system: expression in Escherichia coli and homology to enzymes II from enteric bacteria." Fouet A., Arnaud M., Klier A., Rapoport G. Proc. Natl. Acad. Sci. U.S.A. 84:8773-8777(1987) [PubMed: 3122206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15662 Genomic DNA. Translation: AAA22723.1. X73124 Genomic DNA. Translation: CAA51606.1. AL009126 Genomic DNA. Translation: CAB15830.2. J03006 Genomic DNA. Translation: AAA22728.1.
PIR	A25562.
RefSeq	NP_391683.2.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Genome annotation databases
GeneID	937277.
GenomeReviews	Gene locus BSU38040 in contig AL009126_GR.
KEGG	bsu:BSU38040.
NMPDR	fig|224308.1.peg.3809.
Organism-specific databases
SubtiList	BG10596. sacA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG693902.
PhylomeDB	P07819.
Enzyme and pathway databases
BRENDA	3.2.1.26. 150.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_BACSU

Accession

Primary (citable) accession number: P07819

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	July 28, 2009
Last modified:	January 19, 2010
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)