Reviewed, UniProtKB/Swiss-Prot P07819 (SCRB_BACSU)

Names and origin

Protein names

Recommended name:
    Sucrose-6-phosphate hydrolase
      Short name=Sucrase
    EC=3.2.1.26
Alternative name(s):
    Invertase

Gene names

Name:	sacA
Ordered Locus Names:	BSU38040
ORF Names:	ipa-50d

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	beta-fructofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Sucrose-6-phosphate hydrolase		PRO_0000169870
Regions
	Region	40 – 43	4	Substrate binding By similarity
	Region	102 – 103	2	Substrate binding By similarity
	Region	162 – 163	2	Substrate binding By similarity
Sites
	Active site	43	1	By similarity
	Binding site	59	1	Substrate By similarity
	Binding site	221	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07819-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: B249EB9EE6DEA4C8	FASTA	480	54,889
	10 20 30 40 50 60 MTAHDQELRR RAYEEVEKKE PIANSDPHRQ HFHIMPPVGL LNDPNGVIYW KGSYHVFFQW 70 80 90 100 110 120 QPFQTGHGAK FWGHYTTQDV VNWKREEIAL APSDWFDKNG CYSGSAVTKD DRLYLFYTGN 130 140 150 160 170 180 VRDQDGNRET YQCLAVSDDG LSFEKKGVVA RLPEAILTAH FSRSEVWEHE GTWYMVIGAQ 190 200 210 220 230 240 TENLKGQAVL FASDNLTEWR FLGPITGAGF NGLDDFGYMW ECPDLFSLQG SDVLIVSPQG 250 260 270 280 290 300 LEADGFRYQN VYQSGYFVGR LDYNKPELKH GEFTELDQGF DFYAPQTLED DQGRRILFAW 310 320 330 340 350 360 MAVPDQDEGS HPTIDCHWIH CMTLPRQLTL SGQKLIQQPL PELKAMRRNE KKIHINMHGS 370 380 390 400 410 420 SGALPVEKPE RTEILLEDIH TESGFSISIR GTATFSFHKD EGIVTLERKS FDGKRTEARH 430 440 450 460 470 480 CRIKDLHTVH MFLDASSVEI FINNGEEVFS ARYFPFPGNH EVTASATGKS EMNVGIWTLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the sucrase gene of Bacillus subtilis." Fouet A., Klier A., Rapoport G. Gene 45:221-225(1986) [PubMed: 3100393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325 degrees to 333 degrees." Glaser P., Kunst F., Arnaud M., Coudart M.P., Gonzales W., Hullo M.-F., Ionescu M., Lubochinsky B., Marcelino L., Moszer I., Presecan E., Santana M., Schneider E., Schweizer J., Vertes A., Rapoport G., Danchin A. Mol. Microbiol. 10:371-384(1993) [PubMed: 7934828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Bacillus subtilis sucrose-specific enzyme II of the phosphotransferase system: expression in Escherichia coli and homology to enzymes II from enteric bacteria." Fouet A., Arnaud M., Klier A., Rapoport G. Proc. Natl. Acad. Sci. U.S.A. 84:8773-8777(1987) [PubMed: 3122206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M15662 Genomic DNA. Translation: AAA22723.1. X73124 Genomic DNA. Translation: CAA51606.1. Z99123 Genomic DNA. Translation: CAB15830.1. J03006 Genomic DNA. Translation: AAA22728.1.
PIR	A25562.
RefSeq	NP_391683.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Genome annotation databases
GeneID	937277.
GenomeReviews	Gene locus BSU38040 in contig AL009126_GR.
KEGG	bsu:BSU38040.
NMPDR	fig|224308.1.peg.3809.
Organism-specific databases
SubtiList	BG10596. sacA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P07819.
OMA	P07819. KVWKHND.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3800-MON.
BRENDA	3.2.1.26. 150.
Family and domain databases
InterPro	IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR006232. Suc6P_hydrolase. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
TIGRFAMs	TIGR01322. scrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_BACSU

Accession

Primary (citable) accession number: P07819

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)