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P07813

- SYL_ECOLI

UniProt

P07813 - SYL_ECOLI

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Protein

Leucine--tRNA ligase

Gene
leuS, b0642, JW0637
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: EcoCyc

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:LEUS-MONOMER.
ECOL316407:JW0637-MONOMER.
MetaCyc:LEUS-MONOMER.
SABIO-RKP07813.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leuS
Ordered Locus Names:b0642, JW0637
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10532. leuS.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 860860Leucine--tRNA ligaseUniRule annotationPRO_0000152012Add
BLAST

Proteomic databases

PaxDbiP07813.
PRIDEiP07813.

2D gel databases

SWISS-2DPAGEP07813.

Expressioni

Gene expression databases

GenevestigatoriP07813.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi851814. 1 interaction.
DIPiDIP-10095N.
IntActiP07813. 21 interactions.
MINTiMINT-1226410.
STRINGi511145.b0642.

Structurei

Secondary structure

1
860
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 2014
Turni21 – 244
Beta strandi34 – 396
Beta strandi45 – 473
Helixi50 – 6819
Beta strandi72 – 743
Beta strandi76 – 794
Helixi85 – 928
Beta strandi93 – 953
Helixi97 – 11418
Helixi121 – 1233
Helixi130 – 14516
Beta strandi149 – 1546
Turni160 – 1634
Beta strandi164 – 1663
Helixi168 – 1703
Beta strandi177 – 1793
Beta strandi188 – 1936
Helixi195 – 1984
Helixi199 – 2057
Helixi206 – 2083
Helixi214 – 22411
Beta strandi229 – 2368
Beta strandi240 – 2489
Helixi250 – 2556
Beta strandi258 – 2614
Helixi266 – 2727
Helixi276 – 28712
Helixi292 – 2976
Beta strandi302 – 3109
Turni312 – 3143
Beta strandi317 – 3237
Beta strandi328 – 3314
Beta strandi333 – 3375
Turni339 – 3413
Helixi343 – 35210
Beta strandi371 – 3733
Helixi385 – 3873
Helixi392 – 40514
Beta strandi408 – 4125
Beta strandi423 – 4253
Beta strandi427 – 4293
Beta strandi434 – 4374
Beta strandi442 – 4443
Helixi447 – 4493
Beta strandi462 – 4643
Helixi466 – 4694
Turni471 – 4744
Beta strandi475 – 48511
Turni492 – 4943
Helixi495 – 4973
Helixi499 – 5024
Beta strandi510 – 5123
Helixi515 – 5217
Beta strandi523 – 5286
Helixi531 – 5333
Turni534 – 5363
Helixi537 – 55014
Beta strandi559 – 5646
Beta strandi569 – 5779
Beta strandi583 – 5864
Helixi588 – 5903
Beta strandi592 – 5954
Beta strandi597 – 5993
Beta strandi601 – 6055
Beta strandi613 – 6197
Turni622 – 6254
Helixi630 – 6378
Helixi639 – 64810
Helixi660 – 68223
Helixi691 – 6933
Helixi696 – 71722
Helixi722 – 73716
Helixi744 – 76118
Turni762 – 7643
Helixi766 – 77510
Helixi782 – 7843
Helixi792 – 7954
Beta strandi798 – 8069
Beta strandi809 – 8179
Helixi822 – 83110
Helixi833 – 8364
Turni837 – 8415
Beta strandi846 – 8505
Turni851 – 8533
Beta strandi854 – 8585

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AJGX-ray2.00A/B228-413[»]
2AJHX-ray2.40A/B228-413[»]
2AJIX-ray3.20A/B228-413[»]
3ZGZX-ray2.40A/D1-860[»]
3ZJTX-ray2.20A1-860[»]
3ZJUX-ray2.40A1-860[»]
3ZJVX-ray2.31A1-860[»]
4AQ7X-ray2.50A/D1-860[»]
4ARCX-ray2.00A1-860[»]
4ARIX-ray2.08A1-860[»]
4AS1X-ray2.02A1-860[»]
ProteinModelPortaliP07813.
SMRiP07813. Positions 1-860.

Miscellaneous databases

EvolutionaryTraceiP07813.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionUniRule annotationAdd
BLAST
Motifi619 – 6235"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHISHELW.
OrthoDBiEOG63Z74X.
PhylomeDBiP07813.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07813-1 [UniParc]FASTAAdd to Basket

« Hide

MQEQYRPEEI ESKVQLHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM    50
GHVRNYTIGD VIARYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW 100
TYDNIAYMKN QLKMLGFGYD WSRELATCTP EYYRWEQKFF TELYKKGLVY 150
KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD TKVERKEIPQ WFIKITAYAD 200
ELLNDLDKLD HWPDTVKTMQ RNWIGRSEGV EITFNVNDYD NTLTVYTTRP 250
DTFMGCTYLA VAAGHPLAQK AAENNPELAA FIDECRNTKV AEAEMATMEK 300
KGVDTGFKAV HPLTGEEIPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAS 350
KYGLNIKPVI LAADGSEPDL SQQALTEKGV LFNSGEFNGL DHEAAFNAIA 400
DKLTAMGVGE RKVNYRLRDW GVSRQRYWGA PIPMVTLEDG TVMPTPDDQL 450
PVILPEDVVM DGITSPIKAD PEWAKTTVNG MPALRETDTF DTFMESSWYY 500
ARYTCPQYKE GMLDSEAANY WLPVDIYIGG IEHAIMHLLY FRFFHKLMRD 550
AGMVNSDEPA KQLLCQGMVL ADAFYYVGEN GERNWVSPVD AIVERDEKGR 600
IVKAKDAAGH ELVYTGMSKM SKSKNNGIDP QVMVERYGAD TVRLFMMFAS 650
PADMTLEWQE SGVEGANRFL KRVWKLVYEH TAKGDVAALN VDALTENQKA 700
LRRDVHKTIA KVTDDIGRRQ TFNTAIAAIM ELMNKLAKAP TDGEQDRALM 750
QEALLAVVRM LNPFTPHICF TLWQELKGEG DIDNAPWPVA DEKAMVEDST 800
LVVVQVNGKV RAKITVPVDA TEEQVRERAG QEHLVAKYLD GVTVRKVIYV 850
PGKLLNLVVG 860
Length:860
Mass (Da):97,234
Last modified:November 1, 1997 - v2
Checksum:i856BBEDECC03C83C
GO

Sequence cautioni

The sequence AAB40843.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671R → H in CAA29642. 1 Publication
Sequence conflicti196 – 1961T → N in CAA29642. 1 Publication
Sequence conflicti262 – 2621A → R1 Publication
Sequence conflicti262 – 2621A → R1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06331 Genomic DNA. Translation: CAA29642.1.
U82598 Genomic DNA. Translation: AAB40843.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73743.1.
AP009048 Genomic DNA. Translation: BAA35289.1.
PIRiH64798. SYECL.
RefSeqiNP_415175.1. NC_000913.3.
YP_488933.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73743; AAC73743; b0642.
BAA35289; BAA35289; BAA35289.
GeneIDi12934362.
947497.
KEGGiecj:Y75_p0632.
eco:b0642.
PATRICi32116467. VBIEscCol129921_0673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06331 Genomic DNA. Translation: CAA29642.1 .
U82598 Genomic DNA. Translation: AAB40843.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC73743.1 .
AP009048 Genomic DNA. Translation: BAA35289.1 .
PIRi H64798. SYECL.
RefSeqi NP_415175.1. NC_000913.3.
YP_488933.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2AJG X-ray 2.00 A/B 228-413 [» ]
2AJH X-ray 2.40 A/B 228-413 [» ]
2AJI X-ray 3.20 A/B 228-413 [» ]
3ZGZ X-ray 2.40 A/D 1-860 [» ]
3ZJT X-ray 2.20 A 1-860 [» ]
3ZJU X-ray 2.40 A 1-860 [» ]
3ZJV X-ray 2.31 A 1-860 [» ]
4AQ7 X-ray 2.50 A/D 1-860 [» ]
4ARC X-ray 2.00 A 1-860 [» ]
4ARI X-ray 2.08 A 1-860 [» ]
4AS1 X-ray 2.02 A 1-860 [» ]
ProteinModelPortali P07813.
SMRi P07813. Positions 1-860.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 851814. 1 interaction.
DIPi DIP-10095N.
IntActi P07813. 21 interactions.
MINTi MINT-1226410.
STRINGi 511145.b0642.

2D gel databases

SWISS-2DPAGE P07813.

Proteomic databases

PaxDbi P07813.
PRIDEi P07813.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73743 ; AAC73743 ; b0642 .
BAA35289 ; BAA35289 ; BAA35289 .
GeneIDi 12934362.
947497.
KEGGi ecj:Y75_p0632.
eco:b0642.
PATRICi 32116467. VBIEscCol129921_0673.

Organism-specific databases

EchoBASEi EB0527.
EcoGenei EG10532. leuS.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200747.
KOi K01869.
OMAi HISHELW.
OrthoDBi EOG63Z74X.
PhylomeDBi P07813.

Enzyme and pathway databases

BioCyci EcoCyc:LEUS-MONOMER.
ECOL316407:JW0637-MONOMER.
MetaCyc:LEUS-MONOMER.
SABIO-RK P07813.

Miscellaneous databases

EvolutionaryTracei P07813.
PROi P07813.

Gene expression databases

Genevestigatori P07813.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and nucleotide sequence of the gene for Escherichia coli leucyl-tRNA synthetase."
    Haertlein M., Madern D.
    Nucleic Acids Res. 15:10199-10210(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiSYL_ECOLI
AccessioniPrimary (citable) accession number: P07813
Secondary accession number(s): P77110, P78292
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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