Leucyl-tRNA synthetase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P07813 (SYL_ECOLI)

Last modified June 16, 2009. Version 93. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Leucyl-tRNA synthetase
    EC=6.1.1.4
Alternative name(s):
    Leucine--tRNA ligase
      Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	b0642, JW0637

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	860 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP MF_00049
Subunit structure	Monomer. HAMAP MF_00049
Subcellular location	Cytoplasm. HAMAP MF_00049
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

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Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 860

860

Leucyl-tRNA synthetase HAMAP MF_00049

PRO_0000152012

Regions

Motif

42 – 52

"HIGH" region HAMAP MF_00049

Motif

619 – 623

"KMSKS" region HAMAP MF_00049

Sites

Binding site

622

ATP By similarity

Experimental info

Sequence conflict

R → H in CAA29642. Ref.1

Sequence conflict

196

T → N in CAA29642. Ref.1

Sequence conflict

262

A → R Ref.1

Sequence conflict

262

A → R Ref.3

Secondary structure

860

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P07813-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 856BBEDECC03C83C
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FASTA

860

97,234

        10         20         30         40         50         60 
MQEQYRPEEI ESKVQLHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VIARYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYDNIAYMKN QLKMLGFGYD 

       130        140        150        160        170        180 
WSRELATCTP EYYRWEQKFF TELYKKGLVY KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD 

       190        200        210        220        230        240 
TKVERKEIPQ WFIKITAYAD ELLNDLDKLD HWPDTVKTMQ RNWIGRSEGV EITFNVNDYD 

       250        260        270        280        290        300 
NTLTVYTTRP DTFMGCTYLA VAAGHPLAQK AAENNPELAA FIDECRNTKV AEAEMATMEK 

       310        320        330        340        350        360 
KGVDTGFKAV HPLTGEEIPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAS KYGLNIKPVI 

       370        380        390        400        410        420 
LAADGSEPDL SQQALTEKGV LFNSGEFNGL DHEAAFNAIA DKLTAMGVGE RKVNYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMVTLEDG TVMPTPDDQL PVILPEDVVM DGITSPIKAD PEWAKTTVNG 

       490        500        510        520        530        540 
MPALRETDTF DTFMESSWYY ARYTCPQYKE GMLDSEAANY WLPVDIYIGG IEHAIMHLLY 

       550        560        570        580        590        600 
FRFFHKLMRD AGMVNSDEPA KQLLCQGMVL ADAFYYVGEN GERNWVSPVD AIVERDEKGR 

       610        620        630        640        650        660 
IVKAKDAAGH ELVYTGMSKM SKSKNNGIDP QVMVERYGAD TVRLFMMFAS PADMTLEWQE 

       670        680        690        700        710        720 
SGVEGANRFL KRVWKLVYEH TAKGDVAALN VDALTENQKA LRRDVHKTIA KVTDDIGRRQ 

       730        740        750        760        770        780 
TFNTAIAAIM ELMNKLAKAP TDGEQDRALM QEALLAVVRM LNPFTPHICF TLWQELKGEG 

       790        800        810        820        830        840 
DIDNAPWPVA DEKAMVEDST LVVVQVNGKV RAKITVPVDA TEEQVRERAG QEHLVAKYLD 

       850        860 
GVTVRKVIYV PGKLLNLVVG

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and nucleotide sequence of the gene for Escherichia coli leucyl-tRNA synthetase." Haertlein M., Madern D. Nucleic Acids Res. 15:10199-10210(1987) [PubMed: 3320963] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X06331 Genomic DNA. Translation: CAA29642.1.
U82598 Genomic DNA. Translation: AAB40843.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73743.1.
AP009048 Genomic DNA. Translation: BAA35289.1.

PIR

SYECL. H64798.

RefSeq

AP_001291.1.
NP_415175.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AJG	X-ray	2.00	A/B	228-413	[»]
2AJH	X-ray	2.40	A/B	228-413	[»]
2AJI	X-ray	3.20	A/B	228-413	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:10095N.

2-D gel databases

SWISS-2DPAGE

P07813.

ECO2DBASE

D100.0. 6TH EDITION.

Genome annotation databases

GeneID

947497.

GenomeReviews

Gene locus JW0637 in contig AP009048_GR.
Gene locus b0642 in contig U00096_GR.

KEGG

ecj:JW0637.
eco:b0642.

Organism-specific databases

EchoBASE

EB0527.

EcoGene

EG10532. leuS.

CMR

Search...

Phylogenomic databases

HOGENOM

P07813.

OMA

P07813. MMFASPP.

Enzyme and pathway databases

BioCyc

EcoCyc:LEUS-MON.
MetaCyc:LEUS-MON.

Family and domain databases

HAMAP

MF_00049.
[Tree]

InterPro

IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-synth_Ia_bac/mito.
IPR014729. Rossmann-like_a/b/a_fold.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]

Gene3D

G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.

PANTHER

PTHR11946:SF7. Leu_tRNAsyn_1a. 1 hit.

Pfam

PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]

PRINTS

PR00985. TRNASYNTHLEU.

TIGRFAMs

TIGR00396. leuS_bact. 1 hit.

PROSITE

PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

SYL_ECOLI

Accession

Primary (citable) accession number: P07813
Secondary accession number(s): P77110, P78292

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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