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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei622ATPUniRule annotation1

GO - Molecular functioni

  • aminoacyl-tRNA editing activity Source: InterPro
  • ATP binding Source: UniProtKB-HAMAP
  • leucine-tRNA ligase activity Source: EcoCyc

GO - Biological processi

  • leucyl-tRNA aminoacylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:LEUS-MONOMER.
ECOL316407:JW0637-MONOMER.
MetaCyc:LEUS-MONOMER.
BRENDAi6.1.1.4. 2026.
SABIO-RKP07813.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:b0642, JW0637
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10532. leuS.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001520121 – 860Leucine--tRNA ligaseAdd BLAST860

Proteomic databases

EPDiP07813.
PaxDbiP07813.
PRIDEiP07813.

2D gel databases

SWISS-2DPAGEP07813.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4263356. 16 interactors.
851814. 1 interactor.
DIPiDIP-10095N.
IntActiP07813. 21 interactors.
MINTiMINT-1226410.
STRINGi511145.b0642.

Structurei

Secondary structure

1860
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 20Combined sources14
Turni21 – 24Combined sources4
Beta strandi30 – 32Combined sources3
Beta strandi34 – 39Combined sources6
Beta strandi45 – 47Combined sources3
Helixi50 – 68Combined sources19
Beta strandi72 – 74Combined sources3
Beta strandi76 – 79Combined sources4
Helixi85 – 92Combined sources8
Beta strandi93 – 95Combined sources3
Helixi97 – 114Combined sources18
Helixi121 – 123Combined sources3
Helixi130 – 145Combined sources16
Beta strandi149 – 154Combined sources6
Turni160 – 163Combined sources4
Beta strandi164 – 166Combined sources3
Helixi168 – 170Combined sources3
Beta strandi177 – 179Combined sources3
Beta strandi188 – 193Combined sources6
Helixi195 – 198Combined sources4
Helixi199 – 205Combined sources7
Helixi206 – 208Combined sources3
Helixi214 – 224Combined sources11
Beta strandi229 – 236Combined sources8
Beta strandi240 – 248Combined sources9
Helixi250 – 255Combined sources6
Beta strandi258 – 261Combined sources4
Helixi266 – 272Combined sources7
Helixi276 – 287Combined sources12
Helixi292 – 297Combined sources6
Beta strandi302 – 310Combined sources9
Turni312 – 314Combined sources3
Beta strandi317 – 323Combined sources7
Beta strandi328 – 331Combined sources4
Beta strandi333 – 337Combined sources5
Turni339 – 341Combined sources3
Helixi343 – 352Combined sources10
Beta strandi371 – 373Combined sources3
Helixi385 – 387Combined sources3
Helixi392 – 405Combined sources14
Beta strandi408 – 412Combined sources5
Beta strandi423 – 425Combined sources3
Beta strandi427 – 429Combined sources3
Beta strandi434 – 437Combined sources4
Beta strandi442 – 444Combined sources3
Helixi447 – 449Combined sources3
Beta strandi462 – 464Combined sources3
Helixi466 – 469Combined sources4
Turni471 – 474Combined sources4
Beta strandi475 – 485Combined sources11
Turni492 – 494Combined sources3
Helixi495 – 497Combined sources3
Helixi499 – 502Combined sources4
Beta strandi510 – 512Combined sources3
Helixi515 – 521Combined sources7
Beta strandi523 – 528Combined sources6
Helixi531 – 533Combined sources3
Turni534 – 536Combined sources3
Helixi537 – 550Combined sources14
Beta strandi559 – 564Combined sources6
Beta strandi569 – 577Combined sources9
Beta strandi579 – 581Combined sources3
Beta strandi583 – 586Combined sources4
Helixi588 – 590Combined sources3
Beta strandi592 – 595Combined sources4
Beta strandi597 – 599Combined sources3
Beta strandi601 – 605Combined sources5
Beta strandi613 – 619Combined sources7
Turni622 – 625Combined sources4
Helixi630 – 637Combined sources8
Helixi639 – 648Combined sources10
Helixi660 – 682Combined sources23
Helixi691 – 693Combined sources3
Helixi696 – 717Combined sources22
Helixi722 – 737Combined sources16
Helixi744 – 761Combined sources18
Turni762 – 764Combined sources3
Helixi766 – 775Combined sources10
Helixi782 – 784Combined sources3
Helixi792 – 795Combined sources4
Beta strandi798 – 806Combined sources9
Beta strandi809 – 817Combined sources9
Helixi822 – 831Combined sources10
Helixi833 – 836Combined sources4
Turni837 – 841Combined sources5
Beta strandi846 – 850Combined sources5
Turni851 – 853Combined sources3
Beta strandi854 – 858Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AJGX-ray2.00A/B228-413[»]
2AJHX-ray2.40A/B228-413[»]
2AJIX-ray3.20A/B228-413[»]
3ZGZX-ray2.40A/D1-860[»]
3ZJTX-ray2.20A1-860[»]
3ZJUX-ray2.40A1-860[»]
3ZJVX-ray2.31A1-860[»]
4AQ7X-ray2.50A/D1-860[»]
4ARCX-ray2.00A1-860[»]
4ARIX-ray2.08A1-860[»]
4AS1X-ray2.02A1-860[»]
4CQNX-ray2.50A/D1-860[»]
ProteinModelPortaliP07813.
SMRiP07813.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07813.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 52"HIGH" regionAdd BLAST11
Motifi619 – 623"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200747.
InParanoidiP07813.
KOiK01869.
OMAiHRFIKRL.
PhylomeDBiP07813.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 3 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07813-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQYRPEEI ESKVQLHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VIARYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW
110 120 130 140 150
TYDNIAYMKN QLKMLGFGYD WSRELATCTP EYYRWEQKFF TELYKKGLVY
160 170 180 190 200
KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD TKVERKEIPQ WFIKITAYAD
210 220 230 240 250
ELLNDLDKLD HWPDTVKTMQ RNWIGRSEGV EITFNVNDYD NTLTVYTTRP
260 270 280 290 300
DTFMGCTYLA VAAGHPLAQK AAENNPELAA FIDECRNTKV AEAEMATMEK
310 320 330 340 350
KGVDTGFKAV HPLTGEEIPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAS
360 370 380 390 400
KYGLNIKPVI LAADGSEPDL SQQALTEKGV LFNSGEFNGL DHEAAFNAIA
410 420 430 440 450
DKLTAMGVGE RKVNYRLRDW GVSRQRYWGA PIPMVTLEDG TVMPTPDDQL
460 470 480 490 500
PVILPEDVVM DGITSPIKAD PEWAKTTVNG MPALRETDTF DTFMESSWYY
510 520 530 540 550
ARYTCPQYKE GMLDSEAANY WLPVDIYIGG IEHAIMHLLY FRFFHKLMRD
560 570 580 590 600
AGMVNSDEPA KQLLCQGMVL ADAFYYVGEN GERNWVSPVD AIVERDEKGR
610 620 630 640 650
IVKAKDAAGH ELVYTGMSKM SKSKNNGIDP QVMVERYGAD TVRLFMMFAS
660 670 680 690 700
PADMTLEWQE SGVEGANRFL KRVWKLVYEH TAKGDVAALN VDALTENQKA
710 720 730 740 750
LRRDVHKTIA KVTDDIGRRQ TFNTAIAAIM ELMNKLAKAP TDGEQDRALM
760 770 780 790 800
QEALLAVVRM LNPFTPHICF TLWQELKGEG DIDNAPWPVA DEKAMVEDST
810 820 830 840 850
LVVVQVNGKV RAKITVPVDA TEEQVRERAG QEHLVAKYLD GVTVRKVIYV
860
PGKLLNLVVG
Length:860
Mass (Da):97,234
Last modified:November 1, 1997 - v2
Checksum:i856BBEDECC03C83C
GO

Sequence cautioni

The sequence AAB40843 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67R → H in CAA29642 (PubMed:3320963).Curated1
Sequence conflicti196T → N in CAA29642 (PubMed:3320963).Curated1
Sequence conflicti262A → R (PubMed:3320963).Curated1
Sequence conflicti262A → R (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06331 Genomic DNA. Translation: CAA29642.1.
U82598 Genomic DNA. Translation: AAB40843.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73743.1.
AP009048 Genomic DNA. Translation: BAA35289.1.
PIRiH64798. SYECL.
RefSeqiNP_415175.1. NC_000913.3.
WP_001340834.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73743; AAC73743; b0642.
BAA35289; BAA35289; BAA35289.
GeneIDi947497.
KEGGiecj:JW0637.
eco:b0642.
PATRICi32116467. VBIEscCol129921_0673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06331 Genomic DNA. Translation: CAA29642.1.
U82598 Genomic DNA. Translation: AAB40843.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73743.1.
AP009048 Genomic DNA. Translation: BAA35289.1.
PIRiH64798. SYECL.
RefSeqiNP_415175.1. NC_000913.3.
WP_001340834.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AJGX-ray2.00A/B228-413[»]
2AJHX-ray2.40A/B228-413[»]
2AJIX-ray3.20A/B228-413[»]
3ZGZX-ray2.40A/D1-860[»]
3ZJTX-ray2.20A1-860[»]
3ZJUX-ray2.40A1-860[»]
3ZJVX-ray2.31A1-860[»]
4AQ7X-ray2.50A/D1-860[»]
4ARCX-ray2.00A1-860[»]
4ARIX-ray2.08A1-860[»]
4AS1X-ray2.02A1-860[»]
4CQNX-ray2.50A/D1-860[»]
ProteinModelPortaliP07813.
SMRiP07813.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263356. 16 interactors.
851814. 1 interactor.
DIPiDIP-10095N.
IntActiP07813. 21 interactors.
MINTiMINT-1226410.
STRINGi511145.b0642.

2D gel databases

SWISS-2DPAGEP07813.

Proteomic databases

EPDiP07813.
PaxDbiP07813.
PRIDEiP07813.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73743; AAC73743; b0642.
BAA35289; BAA35289; BAA35289.
GeneIDi947497.
KEGGiecj:JW0637.
eco:b0642.
PATRICi32116467. VBIEscCol129921_0673.

Organism-specific databases

EchoBASEiEB0527.
EcoGeneiEG10532. leuS.

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200747.
InParanoidiP07813.
KOiK01869.
OMAiHRFIKRL.
PhylomeDBiP07813.

Enzyme and pathway databases

BioCyciEcoCyc:LEUS-MONOMER.
ECOL316407:JW0637-MONOMER.
MetaCyc:LEUS-MONOMER.
BRENDAi6.1.1.4. 2026.
SABIO-RKP07813.

Miscellaneous databases

EvolutionaryTraceiP07813.
PROiP07813.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 3 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYL_ECOLI
AccessioniPrimary (citable) accession number: P07813
Secondary accession number(s): P77110, P78292
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.