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P07813

- SYL_ECOLI

UniProt

P07813 - SYL_ECOLI

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei622 – 6221ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: EcoCyc

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: EcoCyc

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:LEUS-MONOMER.
    ECOL316407:JW0637-MONOMER.
    MetaCyc:LEUS-MONOMER.
    SABIO-RKP07813.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:b0642, JW0637
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10532. leuS.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 860860Leucine--tRNA ligasePRO_0000152012Add
    BLAST

    Proteomic databases

    PaxDbiP07813.
    PRIDEiP07813.

    2D gel databases

    SWISS-2DPAGEP07813.

    Expressioni

    Gene expression databases

    GenevestigatoriP07813.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi851814. 1 interaction.
    DIPiDIP-10095N.
    IntActiP07813. 21 interactions.
    MINTiMINT-1226410.
    STRINGi511145.b0642.

    Structurei

    Secondary structure

    1
    860
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 2014
    Turni21 – 244
    Beta strandi34 – 396
    Beta strandi45 – 473
    Helixi50 – 6819
    Beta strandi72 – 743
    Beta strandi76 – 794
    Helixi85 – 928
    Beta strandi93 – 953
    Helixi97 – 11418
    Helixi121 – 1233
    Helixi130 – 14516
    Beta strandi149 – 1546
    Turni160 – 1634
    Beta strandi164 – 1663
    Helixi168 – 1703
    Beta strandi177 – 1793
    Beta strandi188 – 1936
    Helixi195 – 1984
    Helixi199 – 2057
    Helixi206 – 2083
    Helixi214 – 22411
    Beta strandi229 – 2368
    Beta strandi240 – 2489
    Helixi250 – 2556
    Beta strandi258 – 2614
    Helixi266 – 2727
    Helixi276 – 28712
    Helixi292 – 2976
    Beta strandi302 – 3109
    Turni312 – 3143
    Beta strandi317 – 3237
    Beta strandi328 – 3314
    Beta strandi333 – 3375
    Turni339 – 3413
    Helixi343 – 35210
    Beta strandi371 – 3733
    Helixi385 – 3873
    Helixi392 – 40514
    Beta strandi408 – 4125
    Beta strandi423 – 4253
    Beta strandi427 – 4293
    Beta strandi434 – 4374
    Beta strandi442 – 4443
    Helixi447 – 4493
    Beta strandi462 – 4643
    Helixi466 – 4694
    Turni471 – 4744
    Beta strandi475 – 48511
    Turni492 – 4943
    Helixi495 – 4973
    Helixi499 – 5024
    Beta strandi510 – 5123
    Helixi515 – 5217
    Beta strandi523 – 5286
    Helixi531 – 5333
    Turni534 – 5363
    Helixi537 – 55014
    Beta strandi559 – 5646
    Beta strandi569 – 5779
    Beta strandi583 – 5864
    Helixi588 – 5903
    Beta strandi592 – 5954
    Beta strandi597 – 5993
    Beta strandi601 – 6055
    Beta strandi613 – 6197
    Turni622 – 6254
    Helixi630 – 6378
    Helixi639 – 64810
    Helixi660 – 68223
    Helixi691 – 6933
    Helixi696 – 71722
    Helixi722 – 73716
    Helixi744 – 76118
    Turni762 – 7643
    Helixi766 – 77510
    Helixi782 – 7843
    Helixi792 – 7954
    Beta strandi798 – 8069
    Beta strandi809 – 8179
    Helixi822 – 83110
    Helixi833 – 8364
    Turni837 – 8415
    Beta strandi846 – 8505
    Turni851 – 8533
    Beta strandi854 – 8585

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AJGX-ray2.00A/B228-413[»]
    2AJHX-ray2.40A/B228-413[»]
    2AJIX-ray3.20A/B228-413[»]
    3ZGZX-ray2.40A/D1-860[»]
    3ZJTX-ray2.20A1-860[»]
    3ZJUX-ray2.40A1-860[»]
    3ZJVX-ray2.31A1-860[»]
    4AQ7X-ray2.50A/D1-860[»]
    4ARCX-ray2.00A1-860[»]
    4ARIX-ray2.08A1-860[»]
    4AS1X-ray2.02A1-860[»]
    ProteinModelPortaliP07813.
    SMRiP07813. Positions 1-860.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07813.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi42 – 5211"HIGH" regionAdd
    BLAST
    Motifi619 – 6235"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200747.
    KOiK01869.
    OMAiHISHELW.
    OrthoDBiEOG63Z74X.
    PhylomeDBiP07813.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07813-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQEQYRPEEI ESKVQLHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM    50
    GHVRNYTIGD VIARYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW 100
    TYDNIAYMKN QLKMLGFGYD WSRELATCTP EYYRWEQKFF TELYKKGLVY 150
    KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD TKVERKEIPQ WFIKITAYAD 200
    ELLNDLDKLD HWPDTVKTMQ RNWIGRSEGV EITFNVNDYD NTLTVYTTRP 250
    DTFMGCTYLA VAAGHPLAQK AAENNPELAA FIDECRNTKV AEAEMATMEK 300
    KGVDTGFKAV HPLTGEEIPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAS 350
    KYGLNIKPVI LAADGSEPDL SQQALTEKGV LFNSGEFNGL DHEAAFNAIA 400
    DKLTAMGVGE RKVNYRLRDW GVSRQRYWGA PIPMVTLEDG TVMPTPDDQL 450
    PVILPEDVVM DGITSPIKAD PEWAKTTVNG MPALRETDTF DTFMESSWYY 500
    ARYTCPQYKE GMLDSEAANY WLPVDIYIGG IEHAIMHLLY FRFFHKLMRD 550
    AGMVNSDEPA KQLLCQGMVL ADAFYYVGEN GERNWVSPVD AIVERDEKGR 600
    IVKAKDAAGH ELVYTGMSKM SKSKNNGIDP QVMVERYGAD TVRLFMMFAS 650
    PADMTLEWQE SGVEGANRFL KRVWKLVYEH TAKGDVAALN VDALTENQKA 700
    LRRDVHKTIA KVTDDIGRRQ TFNTAIAAIM ELMNKLAKAP TDGEQDRALM 750
    QEALLAVVRM LNPFTPHICF TLWQELKGEG DIDNAPWPVA DEKAMVEDST 800
    LVVVQVNGKV RAKITVPVDA TEEQVRERAG QEHLVAKYLD GVTVRKVIYV 850
    PGKLLNLVVG 860
    Length:860
    Mass (Da):97,234
    Last modified:November 1, 1997 - v2
    Checksum:i856BBEDECC03C83C
    GO

    Sequence cautioni

    The sequence AAB40843.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti67 – 671R → H in CAA29642. (PubMed:3320963)Curated
    Sequence conflicti196 – 1961T → N in CAA29642. (PubMed:3320963)Curated
    Sequence conflicti262 – 2621A → R(PubMed:3320963)Curated
    Sequence conflicti262 – 2621A → R1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06331 Genomic DNA. Translation: CAA29642.1.
    U82598 Genomic DNA. Translation: AAB40843.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC73743.1.
    AP009048 Genomic DNA. Translation: BAA35289.1.
    PIRiH64798. SYECL.
    RefSeqiNP_415175.1. NC_000913.3.
    YP_488933.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73743; AAC73743; b0642.
    BAA35289; BAA35289; BAA35289.
    GeneIDi12934362.
    947497.
    KEGGiecj:Y75_p0632.
    eco:b0642.
    PATRICi32116467. VBIEscCol129921_0673.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06331 Genomic DNA. Translation: CAA29642.1 .
    U82598 Genomic DNA. Translation: AAB40843.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC73743.1 .
    AP009048 Genomic DNA. Translation: BAA35289.1 .
    PIRi H64798. SYECL.
    RefSeqi NP_415175.1. NC_000913.3.
    YP_488933.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2AJG X-ray 2.00 A/B 228-413 [» ]
    2AJH X-ray 2.40 A/B 228-413 [» ]
    2AJI X-ray 3.20 A/B 228-413 [» ]
    3ZGZ X-ray 2.40 A/D 1-860 [» ]
    3ZJT X-ray 2.20 A 1-860 [» ]
    3ZJU X-ray 2.40 A 1-860 [» ]
    3ZJV X-ray 2.31 A 1-860 [» ]
    4AQ7 X-ray 2.50 A/D 1-860 [» ]
    4ARC X-ray 2.00 A 1-860 [» ]
    4ARI X-ray 2.08 A 1-860 [» ]
    4AS1 X-ray 2.02 A 1-860 [» ]
    ProteinModelPortali P07813.
    SMRi P07813. Positions 1-860.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 851814. 1 interaction.
    DIPi DIP-10095N.
    IntActi P07813. 21 interactions.
    MINTi MINT-1226410.
    STRINGi 511145.b0642.

    2D gel databases

    SWISS-2DPAGE P07813.

    Proteomic databases

    PaxDbi P07813.
    PRIDEi P07813.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73743 ; AAC73743 ; b0642 .
    BAA35289 ; BAA35289 ; BAA35289 .
    GeneIDi 12934362.
    947497.
    KEGGi ecj:Y75_p0632.
    eco:b0642.
    PATRICi 32116467. VBIEscCol129921_0673.

    Organism-specific databases

    EchoBASEi EB0527.
    EcoGenei EG10532. leuS.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200747.
    KOi K01869.
    OMAi HISHELW.
    OrthoDBi EOG63Z74X.
    PhylomeDBi P07813.

    Enzyme and pathway databases

    BioCyci EcoCyc:LEUS-MONOMER.
    ECOL316407:JW0637-MONOMER.
    MetaCyc:LEUS-MONOMER.
    SABIO-RK P07813.

    Miscellaneous databases

    EvolutionaryTracei P07813.
    PROi P07813.

    Gene expression databases

    Genevestigatori P07813.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence of the gene for Escherichia coli leucyl-tRNA synthetase."
      Haertlein M., Madern D.
      Nucleic Acids Res. 15:10199-10210(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.

    Entry informationi

    Entry nameiSYL_ECOLI
    AccessioniPrimary (citable) accession number: P07813
    Secondary accession number(s): P77110, P78292
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3