Reviewed, UniProtKB/Swiss-Prot P07811 (PULA_KLEAE)

Names and origin

Protein names

Recommended name:
    Pullulanase
    EC=3.2.1.41
Alternative name(s):
    Alpha-dextrin endo-1,6-alpha-glucosidase
    Pullulan 6-glucanohydrolase

Gene names

Name:

pulA

Organism

Klebsiella aerogenes

Taxonomic identifier

28451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	1096 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.
Subunit structure	Homotrimer. Ref.2
Subcellular location	Cell membrane; Lipid-anchor Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Lipoprotein Palmitate
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro pullulanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19
	Chain	20 – 1096	1077	Pullulanase		PRO_0000001426
Sites
	Active site	694	1	Nucleophile By similarity
	Active site	723	1	Proton donor By similarity
	Active site	851	1	By similarity
Amino acid modifications
	Lipidation	20	1	N-palmitoyl cysteine
	Lipidation	20	1	S-diacylglycerol cysteine

Sequences

	Sequence		Length	Mass (Da)
	P07811-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: FE7D9167CDACFD79	FASTA	1,096	119,336
	10 20 30 40 50 60 MLRYTCHALF LGSLVLLSGC DNSSSSSTSG SPGSPGNPGN PGTPGTPDPQ DVVVRLPDVA 70 80 90 100 110 120 VPGEAVQASA RQAVIHLVDI AGITSSTPAD YATKNLYLWN NETCDALSAP VADWNDVSTT 130 140 150 160 170 180 PTGSDKYGPY WVIPLTKESG SINVIVRDGT NKLIDSGRVS FSDFTDRTVS VIAGNSAVYD 190 200 210 220 230 240 SRADAFRAAF GVALADAHWV DKTTLLWPGG ENKPIVRLYY SHSSKVAADS NGEFSDKYVK 250 260 270 280 290 300 LTPTTVNQQV SMRFPHLASY PAFKLPDDVN VDELLQGDDG GIAESDGILS LSHPGADRRR 310 320 330 340 350 360 AGRYLCRRAE ALSYGAQLTD SGVTFRVWAP TAQQVELVIY SADKKVIASH PMTRDSASGA 370 380 390 400 410 420 WSWQGGSDLK GAFYRYAMTV YHPQSRKVEQ YEVTDPYAHS LSTNSEYSQV VDLNDSALKP 430 440 450 460 470 480 EGWDGLTMPH AQKTKADLAK MTIHESHIRD LSAWDQTVPA ELRGKYLALT AQESNMVQHL 490 500 510 520 530 540 KQLSASGVTH IELLPVFDLA TVNEFSDKVA DIQQPFSRLC EVNSAVKSSE FAGYCDSGST 550 560 570 580 590 600 VEEVLTQLKQ NDSKDNPQVQ ALNTLVAQTD SYNWGYDPFH YTVPEGSYAT DPEGTARIKE 610 620 630 640 650 660 FRTMIQAIKQ DLGMNVIMDV VYNHTNAAGP TDRTSVLDKI VPWYYQRLNE TTGSVESATC 670 680 690 700 710 720 CSDSAPEHRM FAKLIADSLA VWTTDYKIDG FRFDLMGYHP KAQILSAWER IKALNPDIYF 730 740 750 760 770 780 FGEGWDSNQS DRFEIASQIN LKGTGIGTFS DRLRDAVRGG GPFDSGDALR QNQGVGSGAG 790 800 810 820 830 840 VLPNELTTLS DDQARHLADL TRLGMAGNLA DFVLIDKDGA VKRGSEIDYN GAPGGYAADP 850 860 870 880 890 900 TEVVNYVSKH DNQTLWDMIS YKAAQEADLD TRVRMQAVSL ATVMLGQGIA FDQQGSELLR 910 920 930 940 950 960 SKSFTRDSYD SGDWFNRVDY SLQDNNYNVG MPRSSDDGSN YDIIARVKDA VATPGETELK 970 980 990 1000 1010 1020 QMTAFYQELT ALRKSSPLFT LGDGATVMKR VDFRNTGADQ QTGLLVMTID DGMQAGRQSG 1030 1040 1050 1060 1070 1080 QPCRRHRGGD QRRAGKPDAA GLRRHIAPAE RYSAGGGRPV AGERVQVAAD GSVTLPAWSV 1090 AVLELPQASR RALACR

References

[1]	"Entire nucleotide sequence of the pullulanase gene of Klebsiella aerogenes W70." Katsuragi N., Takizawa N., Murooka Y. J. Bacteriol. 169:2301-2306(1987) [PubMed: 3155373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: W70.
[2]	"Collagen-like sequences stabilize homotrimers of a bacterial hydrolase." Charalambous B.M., Keen J.N., McPherson M.J. EMBO J. 7:2903-2909(1988) [PubMed: 2846288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74, SUBUNIT. Strain: FG9.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16187 Genomic DNA. Translation: AAA25124.1. Sequence problems.
PIR	A26879.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
SMR	P07811. Positions 51-1092.
ModBase	Search...
Protein family/group databases
CAZy	CBM41. Carbohydrate-Binding Module Family 41. CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Enzyme and pathway databases
BRENDA	3.2.1.41. 366.
Family and domain databases
InterPro	IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR005323. PUD. IPR011839. Pullul_strch. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02922. CBM_48. 1 hit. PF03714. PUD. 1 hit. [Graphical view]
TIGRFAMs	TIGR02103. pullul_strch. 1 hit.
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PULA_KLEAE

Accession

Primary (citable) accession number: P07811

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)