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P07807 (DYR_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:DFR1
Ordered Locus Names:YOR236W
ORF Names:O5231
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Miscellaneous

Present with 1080 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 211211Dihydrofolate reductase
PRO_0000186378

Regions

Domain7 – 210204DHFR
Nucleotide binding20 – 267NADP By similarity
Nucleotide binding58 – 603NADP By similarity
Nucleotide binding80 – 823NADP By similarity
Nucleotide binding123 – 1308NADP By similarity
Region34 – 396Substrate binding By similarity

Sites

Binding site131NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding site741Substrate By similarity

Experimental info

Sequence conflict271L → V in CAA68779. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P07807 [UniParc].

Last modified July 5, 2005. Version 2.
Checksum: E030CDE7DA481D5D

FASTA21124,261
        10         20         30         40         50         60 
MAGGKIPIVG IVACLQPEMG IGFRGGLPWR LPSEMKYFRQ VTSLTKDPNK KNALIMGRKT 

        70         80         90        100        110        120 
WESIPPKFRP LPNRMNVIIS RSFKDDFVHD KERSIVQSNS LANAIMNLES NFKEHLERIY 

       130        140        150        160        170        180 
VIGGGEVYSQ IFSITDHWLI TKINPLDKNA TPAMDTFLDA KKLEEVFSEQ DPAQLKEFLP 

       190        200        210 
PKVELPETDC DQRYSLEEKG YCFEFTLYNR K 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of the Saccharomyces cerevisiae dihydrofolate reductase gene (DFR1)."
Lagosky P.A., Taylor G.R., Haynes R.H.
Nucleic Acids Res. 15:10355-10371(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequence of the dihydrofolate reductase gene of Saccharomyces cerevisiae."
Fling M.E., Kopf J., Richards C.A.
Gene 63:165-174(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Mapping and sequencing of the dihydrofolate reductase gene (DFR1) of Saccharomyces cerevisiae."
Barclay B.J., Huang T., Nagel M.G., Misener V.L., Game J.C., Wahl G.M.
Gene 63:175-185(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae."
Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B.
Yeast 12:1575-1586(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00887 Genomic DNA. Translation: CAA68779.1.
M18578 Genomic DNA. Translation: AAB59331.1.
M26668 Genomic DNA. Translation: AAA34564.1.
Z75144 Genomic DNA. Translation: CAA99456.1.
BK006948 Genomic DNA. Translation: DAA11005.1.
PIRRDBYD. JT0269.
RefSeqNP_014879.1. NM_001183655.1.

3D structure databases

ProteinModelPortalP07807.
SMRP07807. Positions 5-211.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34628. 44 interactions.
DIPDIP-4120N.
IntActP07807. 4 interactions.
MINTMINT-532257.
STRING4932.YOR236W.

Chemistry

BindingDBP07807.
ChEMBLCHEMBL2576.

Proteomic databases

MaxQBP07807.
PaxDbP07807.
PRIDEP07807.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR236W; YOR236W; YOR236W.
GeneID854411.
KEGGsce:YOR236W.

Organism-specific databases

CYGDYOR236w.
SGDS000005762. DFR1.

Phylogenomic databases

eggNOGCOG0262.
GeneTreeENSGT00390000010283.
HOGENOMHOG000040235.
KOK00287.
OMAKEMKYFR.
OrthoDBEOG7Q8D07.

Enzyme and pathway databases

BioCycYEAST:YOR236W-MONOMER.
UniPathwayUPA00077; UER00158.

Gene expression databases

GenevestigatorP07807.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976601.
PROP07807.

Entry information

Entry nameDYR_YEAST
AccessionPrimary (citable) accession number: P07807
Secondary accession number(s): D6W2T9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 5, 2005
Last modified: June 11, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways