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Protein

Dihydrofolate reductase

Gene

DFR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (DFR1)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei74SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 26NADPBy similarity7
Nucleotide bindingi58 – 60NADPBy similarity3
Nucleotide bindingi80 – 82NADPBy similarity3
Nucleotide bindingi123 – 130NADPBy similarity8

GO - Molecular functioni

  • dihydrofolate reductase activity Source: SGD
  • mRNA binding Source: SGD
  • NADP binding Source: InterPro

GO - Biological processi

  • dihydrofolate metabolic process Source: SGD
  • glycine biosynthetic process Source: InterPro
  • nucleotide biosynthetic process Source: InterPro
  • one-carbon metabolic process Source: UniProtKB-KW
  • tetrahydrofolate biosynthetic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

BioCyciYEAST:YOR236W-MONOMER.
ReactomeiR-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-196757. Metabolism of folate and pterines.
R-SCE-69205. G1/S-Specific Transcription.
UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:DFR1
Ordered Locus Names:YOR236W
ORF Names:O5231
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR236W.
SGDiS000005762. DFR1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2576.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863781 – 211Dihydrofolate reductaseAdd BLAST211

Proteomic databases

MaxQBiP07807.
PRIDEiP07807.

Interactioni

Protein-protein interaction databases

BioGridi34628. 432 interactors.
DIPiDIP-4120N.
IntActiP07807. 4 interactors.
MINTiMINT-532257.

Chemistry databases

BindingDBiP07807.

Structurei

3D structure databases

ProteinModelPortaliP07807.
SMRiP07807.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 210DHFRPROSITE-ProRule annotationAdd BLAST204

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 39Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010283.
HOGENOMiHOG000040235.
InParanoidiP07807.
KOiK00287.
OMAiKMPWRLR.
OrthoDBiEOG092D3GQU.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiView protein in InterPro
IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
PfamiView protein in Pfam
PF00186. DHFR_1. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiView protein in PROSITE
PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P07807-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGGKIPIVG IVACLQPEMG IGFRGGLPWR LPSEMKYFRQ VTSLTKDPNK
60 70 80 90 100
KNALIMGRKT WESIPPKFRP LPNRMNVIIS RSFKDDFVHD KERSIVQSNS
110 120 130 140 150
LANAIMNLES NFKEHLERIY VIGGGEVYSQ IFSITDHWLI TKINPLDKNA
160 170 180 190 200
TPAMDTFLDA KKLEEVFSEQ DPAQLKEFLP PKVELPETDC DQRYSLEEKG
210
YCFEFTLYNR K
Length:211
Mass (Da):24,261
Last modified:July 5, 2005 - v2
Checksum:iE030CDE7DA481D5D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27L → V in CAA68779 (PubMed:2827121).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00887 Genomic DNA. Translation: CAA68779.1.
M18578 Genomic DNA. Translation: AAB59331.1.
M26668 Genomic DNA. Translation: AAA34564.1.
Z75144 Genomic DNA. Translation: CAA99456.1.
BK006948 Genomic DNA. Translation: DAA11005.1.
PIRiJT0269. RDBYD.
RefSeqiNP_014879.1. NM_001183655.1.

Genome annotation databases

EnsemblFungiiYOR236W; YOR236W; YOR236W.
GeneIDi854411.
KEGGisce:YOR236W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00887 Genomic DNA. Translation: CAA68779.1.
M18578 Genomic DNA. Translation: AAB59331.1.
M26668 Genomic DNA. Translation: AAA34564.1.
Z75144 Genomic DNA. Translation: CAA99456.1.
BK006948 Genomic DNA. Translation: DAA11005.1.
PIRiJT0269. RDBYD.
RefSeqiNP_014879.1. NM_001183655.1.

3D structure databases

ProteinModelPortaliP07807.
SMRiP07807.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34628. 432 interactors.
DIPiDIP-4120N.
IntActiP07807. 4 interactors.
MINTiMINT-532257.

Chemistry databases

BindingDBiP07807.
ChEMBLiCHEMBL2576.

Proteomic databases

MaxQBiP07807.
PRIDEiP07807.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR236W; YOR236W; YOR236W.
GeneIDi854411.
KEGGisce:YOR236W.

Organism-specific databases

EuPathDBiFungiDB:YOR236W.
SGDiS000005762. DFR1.

Phylogenomic databases

GeneTreeiENSGT00390000010283.
HOGENOMiHOG000040235.
InParanoidiP07807.
KOiK00287.
OMAiKMPWRLR.
OrthoDBiEOG092D3GQU.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.
BioCyciYEAST:YOR236W-MONOMER.
ReactomeiR-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-196757. Metabolism of folate and pterines.
R-SCE-69205. G1/S-Specific Transcription.

Miscellaneous databases

PROiP07807.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiView protein in InterPro
IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
PfamiView protein in Pfam
PF00186. DHFR_1. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiView protein in PROSITE
PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDYR_YEAST
AccessioniPrimary (citable) accession number: P07807
Secondary accession number(s): D6W2T9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 5, 2005
Last modified: February 15, 2017
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1080 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.