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Protein

Dihydrofolate reductase

Gene

DFR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Miscellaneous

Present with 1080 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (DFR1)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei74SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 26NADPBy similarity7
Nucleotide bindingi58 – 60NADPBy similarity3
Nucleotide bindingi80 – 82NADPBy similarity3
Nucleotide bindingi123 – 130NADPBy similarity8

GO - Molecular functioni

  • dihydrofolate reductase activity Source: SGD
  • mRNA binding Source: SGD

GO - Biological processi

  • dihydrofolate metabolic process Source: SGD
  • one-carbon metabolic process Source: UniProtKB-KW
  • tetrahydrofolate biosynthetic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

BioCyciYEAST:YOR236W-MONOMER
ReactomeiR-SCE-196757 Metabolism of folate and pterines
UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:DFR1
Ordered Locus Names:YOR236W
ORF Names:O5231
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR236W
SGDiS000005762 DFR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2576

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863781 – 211Dihydrofolate reductaseAdd BLAST211

Proteomic databases

MaxQBiP07807
PaxDbiP07807
PRIDEiP07807

Interactioni

Protein-protein interaction databases

BioGridi34628, 440 interactors
DIPiDIP-4120N
IntActiP07807, 4 interactors
MINTiP07807
STRINGi4932.YOR236W

Chemistry databases

BindingDBiP07807

Structurei

3D structure databases

ProteinModelPortaliP07807
SMRiP07807
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 210DHFRPROSITE-ProRule annotationAdd BLAST204

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 39Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010283
HOGENOMiHOG000040235
InParanoidiP07807
KOiK00287
OMAiMFLLLNC
OrthoDBiEOG092C4LJX

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P07807-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGGKIPIVG IVACLQPEMG IGFRGGLPWR LPSEMKYFRQ VTSLTKDPNK
60 70 80 90 100
KNALIMGRKT WESIPPKFRP LPNRMNVIIS RSFKDDFVHD KERSIVQSNS
110 120 130 140 150
LANAIMNLES NFKEHLERIY VIGGGEVYSQ IFSITDHWLI TKINPLDKNA
160 170 180 190 200
TPAMDTFLDA KKLEEVFSEQ DPAQLKEFLP PKVELPETDC DQRYSLEEKG
210
YCFEFTLYNR K
Length:211
Mass (Da):24,261
Last modified:July 5, 2005 - v2
Checksum:iE030CDE7DA481D5D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27L → V in CAA68779 (PubMed:2827121).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00887 Genomic DNA Translation: CAA68779.1
M18578 Genomic DNA Translation: AAB59331.1
M26668 Genomic DNA Translation: AAA34564.1
Z75144 Genomic DNA Translation: CAA99456.1
BK006948 Genomic DNA Translation: DAA11005.1
PIRiJT0269 RDBYD
RefSeqiNP_014879.1, NM_001183655.1

Genome annotation databases

EnsemblFungiiYOR236W; YOR236W; YOR236W
GeneIDi854411
KEGGisce:YOR236W

Similar proteinsi

Entry informationi

Entry nameiDYR_YEAST
AccessioniPrimary (citable) accession number: P07807
Secondary accession number(s): D6W2T9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 5, 2005
Last modified: May 23, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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