Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P07807

- DYR_YEAST

UniProt

P07807 - DYR_YEAST

Protein

Dihydrofolate reductase

Gene

DFR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (05 Jul 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei74 – 741SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi20 – 267NADPBy similarity
    Nucleotide bindingi58 – 603NADPBy similarity
    Nucleotide bindingi80 – 823NADPBy similarity
    Nucleotide bindingi123 – 1308NADPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: SGD
    2. mRNA binding Source: SGD
    3. NADP binding Source: InterPro

    GO - Biological processi

    1. dihydrofolate metabolic process Source: SGD
    2. glycine biosynthetic process Source: InterPro
    3. nucleotide biosynthetic process Source: InterPro
    4. one-carbon metabolic process Source: UniProtKB-KW
    5. tetrahydrofolate biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciYEAST:YOR236W-MONOMER.
    ReactomeiREACT_189008. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
    REACT_189247. G1/S-Specific Transcription.
    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase (EC:1.5.1.3)
    Gene namesi
    Name:DFR1
    Ordered Locus Names:YOR236W
    ORF Names:O5231
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR236w.
    SGDiS000005762. DFR1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 211211Dihydrofolate reductasePRO_0000186378Add
    BLAST

    Proteomic databases

    MaxQBiP07807.
    PaxDbiP07807.
    PRIDEiP07807.

    Expressioni

    Gene expression databases

    GenevestigatoriP07807.

    Interactioni

    Protein-protein interaction databases

    BioGridi34628. 44 interactions.
    DIPiDIP-4120N.
    IntActiP07807. 4 interactions.
    MINTiMINT-532257.
    STRINGi4932.YOR236W.

    Structurei

    3D structure databases

    ProteinModelPortaliP07807.
    SMRiP07807. Positions 5-211.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 210204DHFRPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni34 – 396Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated
    Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0262.
    GeneTreeiENSGT00390000010283.
    HOGENOMiHOG000040235.
    KOiK00287.
    OMAiKEMKYFR.
    OrthoDBiEOG7Q8D07.

    Family and domain databases

    Gene3Di3.40.430.10. 1 hit.
    InterProiIPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    [Graphical view]
    PRINTSiPR00070. DHFR.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07807-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGGKIPIVG IVACLQPEMG IGFRGGLPWR LPSEMKYFRQ VTSLTKDPNK    50
    KNALIMGRKT WESIPPKFRP LPNRMNVIIS RSFKDDFVHD KERSIVQSNS 100
    LANAIMNLES NFKEHLERIY VIGGGEVYSQ IFSITDHWLI TKINPLDKNA 150
    TPAMDTFLDA KKLEEVFSEQ DPAQLKEFLP PKVELPETDC DQRYSLEEKG 200
    YCFEFTLYNR K 211
    Length:211
    Mass (Da):24,261
    Last modified:July 5, 2005 - v2
    Checksum:iE030CDE7DA481D5D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti27 – 271L → V in CAA68779. (PubMed:2827121)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00887 Genomic DNA. Translation: CAA68779.1.
    M18578 Genomic DNA. Translation: AAB59331.1.
    M26668 Genomic DNA. Translation: AAA34564.1.
    Z75144 Genomic DNA. Translation: CAA99456.1.
    BK006948 Genomic DNA. Translation: DAA11005.1.
    PIRiJT0269. RDBYD.
    RefSeqiNP_014879.1. NM_001183655.1.

    Genome annotation databases

    EnsemblFungiiYOR236W; YOR236W; YOR236W.
    GeneIDi854411.
    KEGGisce:YOR236W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00887 Genomic DNA. Translation: CAA68779.1 .
    M18578 Genomic DNA. Translation: AAB59331.1 .
    M26668 Genomic DNA. Translation: AAA34564.1 .
    Z75144 Genomic DNA. Translation: CAA99456.1 .
    BK006948 Genomic DNA. Translation: DAA11005.1 .
    PIRi JT0269. RDBYD.
    RefSeqi NP_014879.1. NM_001183655.1.

    3D structure databases

    ProteinModelPortali P07807.
    SMRi P07807. Positions 5-211.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34628. 44 interactions.
    DIPi DIP-4120N.
    IntActi P07807. 4 interactions.
    MINTi MINT-532257.
    STRINGi 4932.YOR236W.

    Chemistry

    BindingDBi P07807.
    ChEMBLi CHEMBL2576.

    Proteomic databases

    MaxQBi P07807.
    PaxDbi P07807.
    PRIDEi P07807.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR236W ; YOR236W ; YOR236W .
    GeneIDi 854411.
    KEGGi sce:YOR236W.

    Organism-specific databases

    CYGDi YOR236w.
    SGDi S000005762. DFR1.

    Phylogenomic databases

    eggNOGi COG0262.
    GeneTreei ENSGT00390000010283.
    HOGENOMi HOG000040235.
    KOi K00287.
    OMAi KEMKYFR.
    OrthoDBi EOG7Q8D07.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .
    BioCyci YEAST:YOR236W-MONOMER.
    Reactomei REACT_189008. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
    REACT_189247. G1/S-Specific Transcription.

    Miscellaneous databases

    NextBioi 976601.
    PROi P07807.

    Gene expression databases

    Genevestigatori P07807.

    Family and domain databases

    Gene3Di 3.40.430.10. 1 hit.
    InterProi IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00070. DHFR.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the Saccharomyces cerevisiae dihydrofolate reductase gene (DFR1)."
      Lagosky P.A., Taylor G.R., Haynes R.H.
      Nucleic Acids Res. 15:10355-10371(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Nucleotide sequence of the dihydrofolate reductase gene of Saccharomyces cerevisiae."
      Fling M.E., Kopf J., Richards C.A.
      Gene 63:165-174(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Mapping and sequencing of the dihydrofolate reductase gene (DFR1) of Saccharomyces cerevisiae."
      Barclay B.J., Huang T., Nagel M.G., Misener V.L., Game J.C., Wahl G.M.
      Gene 63:175-185(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae."
      Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B.
      Yeast 12:1575-1586(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDYR_YEAST
    AccessioniPrimary (citable) accession number: P07807
    Secondary accession number(s): D6W2T9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1080 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3