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P07807

- DYR_YEAST

UniProt

P07807 - DYR_YEAST

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Protein

Dihydrofolate reductase

Gene

DFR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NADP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei74 – 741SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 267NADPBy similarity
Nucleotide bindingi58 – 603NADPBy similarity
Nucleotide bindingi80 – 823NADPBy similarity
Nucleotide bindingi123 – 1308NADPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: SGD
  2. mRNA binding Source: SGD
  3. NADP binding Source: InterPro

GO - Biological processi

  1. dihydrofolate metabolic process Source: SGD
  2. glycine biosynthetic process Source: InterPro
  3. nucleotide biosynthetic process Source: InterPro
  4. one-carbon metabolic process Source: UniProtKB-KW
  5. tetrahydrofolate biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciYEAST:YOR236W-MONOMER.
ReactomeiREACT_189008. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
REACT_189247. G1/S-Specific Transcription.
UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:DFR1
Ordered Locus Names:YOR236W
ORF Names:O5231
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR236w.
SGDiS000005762. DFR1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Dihydrofolate reductasePRO_0000186378Add
BLAST

Proteomic databases

MaxQBiP07807.
PaxDbiP07807.
PRIDEiP07807.

Expressioni

Gene expression databases

GenevestigatoriP07807.

Interactioni

Protein-protein interaction databases

BioGridi34628. 45 interactions.
DIPiDIP-4120N.
IntActiP07807. 4 interactions.
MINTiMINT-532257.
STRINGi4932.YOR236W.

Structurei

3D structure databases

ProteinModelPortaliP07807.
SMRiP07807. Positions 5-211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 210204DHFRPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 396Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0262.
GeneTreeiENSGT00390000010283.
HOGENOMiHOG000040235.
InParanoidiP07807.
KOiK00287.
OMAiKEMKYFR.
OrthoDBiEOG7Q8D07.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07807-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGGKIPIVG IVACLQPEMG IGFRGGLPWR LPSEMKYFRQ VTSLTKDPNK
60 70 80 90 100
KNALIMGRKT WESIPPKFRP LPNRMNVIIS RSFKDDFVHD KERSIVQSNS
110 120 130 140 150
LANAIMNLES NFKEHLERIY VIGGGEVYSQ IFSITDHWLI TKINPLDKNA
160 170 180 190 200
TPAMDTFLDA KKLEEVFSEQ DPAQLKEFLP PKVELPETDC DQRYSLEEKG
210
YCFEFTLYNR K
Length:211
Mass (Da):24,261
Last modified:July 5, 2005 - v2
Checksum:iE030CDE7DA481D5D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271L → V in CAA68779. (PubMed:2827121)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00887 Genomic DNA. Translation: CAA68779.1.
M18578 Genomic DNA. Translation: AAB59331.1.
M26668 Genomic DNA. Translation: AAA34564.1.
Z75144 Genomic DNA. Translation: CAA99456.1.
BK006948 Genomic DNA. Translation: DAA11005.1.
PIRiJT0269. RDBYD.
RefSeqiNP_014879.1. NM_001183655.1.

Genome annotation databases

EnsemblFungiiYOR236W; YOR236W; YOR236W.
GeneIDi854411.
KEGGisce:YOR236W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00887 Genomic DNA. Translation: CAA68779.1 .
M18578 Genomic DNA. Translation: AAB59331.1 .
M26668 Genomic DNA. Translation: AAA34564.1 .
Z75144 Genomic DNA. Translation: CAA99456.1 .
BK006948 Genomic DNA. Translation: DAA11005.1 .
PIRi JT0269. RDBYD.
RefSeqi NP_014879.1. NM_001183655.1.

3D structure databases

ProteinModelPortali P07807.
SMRi P07807. Positions 5-211.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34628. 45 interactions.
DIPi DIP-4120N.
IntActi P07807. 4 interactions.
MINTi MINT-532257.
STRINGi 4932.YOR236W.

Chemistry

BindingDBi P07807.
ChEMBLi CHEMBL2576.

Proteomic databases

MaxQBi P07807.
PaxDbi P07807.
PRIDEi P07807.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR236W ; YOR236W ; YOR236W .
GeneIDi 854411.
KEGGi sce:YOR236W.

Organism-specific databases

CYGDi YOR236w.
SGDi S000005762. DFR1.

Phylogenomic databases

eggNOGi COG0262.
GeneTreei ENSGT00390000010283.
HOGENOMi HOG000040235.
InParanoidi P07807.
KOi K00287.
OMAi KEMKYFR.
OrthoDBi EOG7Q8D07.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .
BioCyci YEAST:YOR236W-MONOMER.
Reactomei REACT_189008. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
REACT_189247. G1/S-Specific Transcription.

Miscellaneous databases

NextBioi 976601.
PROi P07807.

Gene expression databases

Genevestigatori P07807.

Family and domain databases

Gene3Di 3.40.430.10. 1 hit.
InterProi IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
[Graphical view ]
PRINTSi PR00070. DHFR.
SUPFAMi SSF53597. SSF53597. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the Saccharomyces cerevisiae dihydrofolate reductase gene (DFR1)."
    Lagosky P.A., Taylor G.R., Haynes R.H.
    Nucleic Acids Res. 15:10355-10371(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the dihydrofolate reductase gene of Saccharomyces cerevisiae."
    Fling M.E., Kopf J., Richards C.A.
    Gene 63:165-174(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Mapping and sequencing of the dihydrofolate reductase gene (DFR1) of Saccharomyces cerevisiae."
    Barclay B.J., Huang T., Nagel M.G., Misener V.L., Game J.C., Wahl G.M.
    Gene 63:175-185(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae."
    Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B.
    Yeast 12:1575-1586(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDYR_YEAST
AccessioniPrimary (citable) accession number: P07807
Secondary accession number(s): D6W2T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 5, 2005
Last modified: October 29, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1080 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3