Reviewed,
UniProtKB/Swiss-Prot P07806 (SYV_YEAST)
Last modified
November 24, 2009.
Version 108.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Valyl-tRNA synthetase, mitochondrial EC=6.1.1.9 Alternative name(s): Valine--tRNA ligase Short name=ValRS | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 1104 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). |
| Subcellular location | |
| Miscellaneous | Present with 358 molecules/cell in log phase SD medium. Ref.4 |
| Sequence similarities | Belongs to the class-I aminoacyl-tRNA synthetase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein biosynthesis |
| Cellular component | Cytoplasm Mitochondrion |
| Coding sequence diversity | Alternative initiation |
| Domain | Transit peptide |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Aminoacyl-tRNA synthetase Ligase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | valyl-tRNA aminoacylation Inferred from direct assay. Source: SGD |
| Cellular component | mitochondrion Ref.3 Inferred from direct assay. Source: SGD |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein bindingInferred from physical interaction. Source: IntAct valine-tRNA ligase activityInferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Binary interactions
Alternative products
| This entry describes 2 isoforms produced by alternative initiation. [Align] [Select] | ||||||
| Isoform Mitochondrial (identifier: P07806-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Cytoplasmic (identifier: P07806-2) The sequence of this isoform differs from the canonical sequence as follows: 1-46: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 47 | 47 | Mitochondrion | ||||||
| Chain | 48 – 1104 | 1057 | Valyl-tRNA synthetase, mitochondrial | PRO_0000035839 | |||||
Regions | |||||||||
| Motif | 190 – 200 | 11 | "HIGH" region | ||||||
| Motif | 703 – 707 | 5 | "KMSKS" region | ||||||
Sites | |||||||||
| Binding site | 706 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 73 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 294 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 332 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 707 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 1003 | 1 | Phosphothreonine Ref.5 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 46 | 46 | Missing in isoform Cytoplasmic. | VSP_018910 | |||||
Experimental info | |||||||||
| Sequence conflict | 147 | 1 | A → G in AAA35207. Ref.1 | ||||||
| Sequence conflict | 540 | 1 | R → K in AAA35207. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structure of the yeast valyl-tRNA synthetase gene (VASI) and the homology of its translated amino acid sequence with Escherichia coli isoleucyl-tRNA synthetase." Jordana X., Chatton B., Paz-Weisshaar M., Buhler J.-M., Cramer F., Ebel J.-P., Fasiolo F. J. Biol. Chem. 262:7189-7194(1987) [PubMed: 3294828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.  Kleine K.Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [3] | "The yeast VAS1 gene encodes both mitochondrial and cytoplasmic valyl-tRNA synthetases." Chatton B., Walter P., Ebel J.-P., Lacroute F., Fasiolo F. J. Biol. Chem. 263:52-57(1988) [PubMed: 3275649] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-78. |
| [4] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [5] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73; SER-294; SER-332; SER-707 AND THR-1003, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| J02719 Genomic DNA. Translation: AAA35207.1. Z72879 Genomic DNA. Translation: CAA97097.1. M18392 Genomic DNA. Translation: AAA35205.1. M18392 Genomic DNA. Translation: AAA35206.1. | |
| PIR | SYBYVT. S64389. |
| RefSeq | NP_011608.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:6328N. |
| IntAct | P07806. 73 interactions. |
| STRING | P07806. |
Proteomic databases | |
| PeptideAtlas | P07806. |
| PRIDE | P07806. |
Genome annotation databases | |
| Ensembl | YGR094W; YGR094W; YGR094W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 852986. |
| KEGG | sce:YGR094W. |
| NMPDR | fig|4932.3.peg.2725. |
Organism-specific databases | |
| CYGD | YGR094w. |
| SGD | S000003326. VAS1. |
Phylogenomic databases | |
| HOGENOM | P07806. |
| OMA | WPDETPE |
| OrthoDB | EOG9QRJMW |
Enzyme and pathway databases | |
| BRENDA | 6.1.1.9. 250. |
Gene expression databases | |
| ArrayExpress | P07806. |
| Genevestigator | P07806. |
| GermOnline | YGR094W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR010978. tRNA_bd_arm. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR002303. Val-tRNA_synth_Ia. IPR019754. Val-tRNA_synth_Ia_N. IPR019499. Val-tRNA_synth_Ia_tRNA-bd. IPR009008. Val/Leu/Ile-tRNA-synth_Ia_edit. [Graphical view] |
| Gene3D | G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. |
| PANTHER | PTHR11946:SF5. tRNA-synt_val. 1 hit. |
| Pfam | PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF10458. Val_tRNA-synt_C. 1 hit. [Graphical view] |
| PRINTS | PR00986. TRNASYNTHVAL. |
| TIGRFAMs | TIGR00422. valS. 1 hit. |
| PROSITE | PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 972803. |
Entry information
| Entry name | SYV_YEAST | ||||||||
| Accession | Primary (citable) accession number: P07806 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Aminoacyl-tRNA synthetases List of aminoacyl-tRNA synthetase entries |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VII Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names |
