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Protein

Valine--tRNA ligase, mitochondrial

Gene

VAS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei706ATPBy similarity1

GO - Molecular functioni

  • aminoacyl-tRNA editing activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • valine-tRNA ligase activity Source: SGD

GO - Biological processi

  • valyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30804-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligase, mitochondrial (EC:6.1.1.9)
Alternative name(s):
Valyl-tRNA synthetase
Short name:
ValRS
Gene namesi
Name:VAS1
Ordered Locus Names:YGR094W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR094W.
SGDiS000003326. VAS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47MitochondrionAdd BLAST47
ChainiPRO_000003583948 – 1104Valine--tRNA ligase, mitochondrialAdd BLAST1057

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73PhosphoserineCombined sources1
Modified residuei294PhosphoserineCombined sources1
Modified residuei332PhosphoserineCombined sources1
Modified residuei707PhosphoserineCombined sources1
Modified residuei1003PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP07806.

PTM databases

iPTMnetiP07806.

Interactioni

Protein-protein interaction databases

BioGridi33337. 47 interactors.
DIPiDIP-6328N.
IntActiP07806. 8 interactors.
MINTiMINT-652049.

Structurei

3D structure databases

ProteinModelPortaliP07806.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi190 – 200"HIGH" regionAdd BLAST11
Motifi703 – 707"KMSKS" region5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000074727.
HOGENOMiHOG000020094.
InParanoidiP07806.
KOiK01873.
OMAiNEMTIPT.
OrthoDBiEOG092C0H3K.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 3 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: P07806-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNKWLNTLSK TFTFRLLNCH YRRSLPLCQN FSLKKSLTHN QVRFFKMSDL
60 70 80 90 100
DNLPPVDPKT GEVIINPLKE DGSPKTPKEI EKEKKKAEKL LKFAAKQAKK
110 120 130 140 150
NAAATTGASQ KKPKKKKEVE PIPEFIDKTV PGEKKILVSL DDPALKAYNP
160 170 180 190 200
ANVESSWYDW WIKTGVFEPE FTADGKVKPE GVFCIPAPPP NVTGALHIGH
210 220 230 240 250
ALTIAIQDSL IRYNRMKGKT VLFLPGFDHA GIATQSVVEK QIWAKDRKTR
260 270 280 290 300
HDYGREAFVG KVWEWKEEYH SRIKNQIQKL GASYDWSREA FTLSPELTKS
310 320 330 340 350
VEEAFVRLHD EGVIYRASRL VNWSVKLNTA ISNLEVENKD VKSRTLLSVP
360 370 380 390 400
GYDEKVEFGV LTSFAYPVIG SDEKLIIATT RPETIFGDTA VAVHPDDDRY
410 420 430 440 450
KHLHGKFIQH PFLPRKIPII TDKEAVDMEF GTGAVKITPA HDQNDYNTGK
460 470 480 490 500
RHNLEFINIL TDDGLLNEEC GPEWQGMKRF DARKKVIEQL KEKNLYVGQE
510 520 530 540 550
DNEMTIPTCS RSGDIIEPLL KPQWWVSQSE MAKDAIKVVR DGQITITPKS
560 570 580 590 600
SEAEYFHWLG NIQDWCISRQ LWWGHRCPVY FINIEGEEHD RIDGDYWVAG
610 620 630 640 650
RSMEEAEKKA AAKYPNSKFT LEQDEDVLDT WFSSGLWPFS TLGWPEKTKD
660 670 680 690 700
METFYPFSML ETGWDILFFW VTRMILLGLK LTGSVPFKEV FCHSLVRDAQ
710 720 730 740 750
GRKMSKSLGN VIDPLDVITG IKLDDLHAKL LQGNLDPREV EKAKIGQKES
760 770 780 790 800
YPNGIPQCGT DAMRFALCAY TTGGRDINLD ILRVEGYRKF CNKIYQATKF
810 820 830 840 850
ALMRLGDDYQ PPATEGLSGN ESLVEKWILH KLTETSKIVN EALDKRDFLT
860 870 880 890 900
STSSIYEFWY LICDVYIENS KYLIQEGSAI EKKSAKDTLY ILLDNALKLI
910 920 930 940 950
HPFMPFISEE MWQRLPKRST EKAASIVKAS YPVYVSEYDD VKSANAYDLV
960 970 980 990 1000
LNITKEARSL LSEYNILKNG KVFVESNHEE YFKTAEDQKD SIVSLIKAID
1010 1020 1030 1040 1050
EVTVVRDASE IPEGCVLQSV NPEVNVHLLV KGHVDIDAEI AKVQKKLEKA
1060 1070 1080 1090 1100
KKSKNGIEQT INSKDYETKA NTQAKEANKS KLDNTVAEIE GLEATIENLK

RLKL
Length:1,104
Mass (Da):125,770
Last modified:October 1, 1996 - v2
Checksum:i6493AEF37ECD4A7C
GO
Isoform Cytoplasmic (identifier: P07806-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Note: Produced by alternative initiation at Met-47 of isoform Mitochondrial.
Show »
Length:1,058
Mass (Da):120,126
Checksum:i123D6E8A5494FB73
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti147A → G in AAA35207 (PubMed:3294828).Curated1
Sequence conflicti540R → K in AAA35207 (PubMed:3294828).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189101 – 46Missing in isoform Cytoplasmic. CuratedAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02719 Genomic DNA. Translation: AAA35207.1.
Z72879 Genomic DNA. Translation: CAA97097.1.
M18392 Genomic DNA. Translation: AAA35205.1.
M18392 Genomic DNA. Translation: AAA35206.1.
BK006941 Genomic DNA. Translation: DAA08187.1.
PIRiS64389. SYBYVT.
RefSeqiNP_011608.1. NM_001181223.1. [P07806-1]

Genome annotation databases

EnsemblFungiiYGR094W; YGR094W; YGR094W. [P07806-1]
GeneIDi852986.
KEGGisce:YGR094W.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02719 Genomic DNA. Translation: AAA35207.1.
Z72879 Genomic DNA. Translation: CAA97097.1.
M18392 Genomic DNA. Translation: AAA35205.1.
M18392 Genomic DNA. Translation: AAA35206.1.
BK006941 Genomic DNA. Translation: DAA08187.1.
PIRiS64389. SYBYVT.
RefSeqiNP_011608.1. NM_001181223.1. [P07806-1]

3D structure databases

ProteinModelPortaliP07806.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33337. 47 interactors.
DIPiDIP-6328N.
IntActiP07806. 8 interactors.
MINTiMINT-652049.

PTM databases

iPTMnetiP07806.

Proteomic databases

PRIDEiP07806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR094W; YGR094W; YGR094W. [P07806-1]
GeneIDi852986.
KEGGisce:YGR094W.

Organism-specific databases

EuPathDBiFungiDB:YGR094W.
SGDiS000003326. VAS1.

Phylogenomic databases

GeneTreeiENSGT00550000074727.
HOGENOMiHOG000020094.
InParanoidiP07806.
KOiK01873.
OMAiNEMTIPT.
OrthoDBiEOG092C0H3K.

Enzyme and pathway databases

BioCyciYEAST:G3O-30804-MONOMER.

Miscellaneous databases

PROiP07806.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 3 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYV_YEAST
AccessioniPrimary (citable) accession number: P07806
Secondary accession number(s): D6VUM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 172 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.