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Protein

Glutamine synthetase

Gene

glnA

Organism
Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulationi

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive (By similarity).By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
Gene namesi
Name:glnA
OrganismiAcidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
Taxonomic identifieri920 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Glutamine synthetasePRO_0000153274Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei397 – 3971O-AMP-tyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP07804.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.

Structurei

3D structure databases

ProteinModelPortaliP07804.
SMRiP07804. Positions 4-468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYSPSDVVK LIQEKDIKFI DFRFTDTKGK EQHVSVPGHV IEEDTFTEGK
60 70 80 90 100
AFDGSSIAGW KGINESDMIL LPDPDSAVLD PFMDETTLLL RCDVIEPATG
110 120 130 140 150
QGYERDPRSV AKRAEIYLKS TGIADTSFFG PEFFVFDSVT WNIDMSGCAY
160 170 180 190 200
KVDAEEAAWN SGKEYESGNM GHRLGVKGGY FPVPPVDSAQ DLRSAMCLAM
210 220 230 240 250
EEMGLKVEVH HHEVATAGQH EIGVGFNTLT PRRMRCKILK YVVHNVAAVR
260 270 280 290 300
QTATFMPKPV VGDNGSGMHV HQSLGKDGKN IFAGDLYGGL SEIALYYIGG
310 320 330 340 350
IIKHAKAVNA LTNPSTNSYK RLVPHFEAPV LLAYSAKNRS ASIRIPYVNS
360 370 380 390 400
PKARRIEVRF PDSTANPYLA FSAMLMAGLD GIQNKIHPAT AMDKNLYDLP
410 420 430 440 450
AEEQANIPGV AASLEEALRA LEADHDFLMK GGVFSESWLE GYLDVKWAEV
460
QTLRVTTHPV EFQMYYSL
Length:468
Mass (Da):51,614
Last modified:August 1, 1988 - v1
Checksum:i24710B272B3A7054
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16626 Genomic DNA. Translation: AAA27370.1.
PIRiA29460. AJBCQF.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16626 Genomic DNA. Translation: AAA27370.1.
PIRiA29460. AJBCQF.

3D structure databases

ProteinModelPortaliP07804.
SMRiP07804. Positions 4-468.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP07804.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the glutamine synthetase gene and its controlling region from the acidophilic autotroph Thiobacillus ferrooxidans."
    Rawlings D.E., Jones W.A., O'Neill E.G., Woods D.R.
    Gene 53:211-217(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGLNA_ACIFR
AccessioniPrimary (citable) accession number: P07804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: January 7, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.