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P07799

- TOP1_SCHPO

UniProt

P07799 - TOP1_SCHPO

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Protein
DNA topoisomerase 1
Gene
top1, SPBC1703.14c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei343 – 3431Interaction with DNA By similarity
Sitei391 – 3911Interaction with DNA By similarity
Sitei422 – 4221Interaction with DNA By similarity
Sitei479 – 4791Interaction with DNA By similarity
Sitei505 – 5051Interaction with DNA By similarity
Sitei548 – 5481Interaction with DNA By similarity
Sitei605 – 6051Interaction with DNA By similarity
Active sitei773 – 7731O-(3'-phospho-DNA)-tyrosine intermediate1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: PomBase
  3. DNA topoisomerase type I activity Source: PomBase
  4. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

GO - Biological processi

  1. DNA topological change Source: PomBase
  2. chromatin assembly or disassembly Source: PomBase
  3. chromatin organization Source: PomBase
  4. mitotic chromosome condensation Source: PomBase
  5. transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Gene namesi
Name:top1
ORF Names:SPBC1703.14c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC1703.14c.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. nuclear chromatin Source: PomBase
  3. nucleolus Source: PomBase
  4. nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 814814DNA topoisomerase 1
PRO_0000145209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521Phosphoserine1 Publication
Modified residuei54 – 541Phosphoserine1 Publication
Modified residuei136 – 1361Phosphoserine1 Publication
Modified residuei138 – 1381Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07799.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi276575. 81 interactions.
MINTiMINT-4686963.
STRINGi4896.SPBC1703.14c-1.

Structurei

3D structure databases

ProteinModelPortaliP07799.
SMRiP07799. Positions 180-608, 762-807.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni404 – 4052Interaction with DNA By similarity
Regioni467 – 4726Interaction with DNA By similarity
Regioni559 – 5613Interaction with DNA By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3569.
HOGENOMiHOG000105469.
KOiK03163.
OMAiKSVDEDW.
OrthoDBiEOG7966R0.
PhylomeDBiP07799.

Family and domain databases

Gene3Di1.10.10.41. 1 hit.
1.10.132.10. 2 hits.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamiPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SMARTiSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07799-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSDSDSVS LSIRRRQRRG SSKRISMKES DEESDSSENH PLSESLNKKS    50
KSESDEDDIP IRKRRASSKK NMSNSSSKKR AKVMGNGGLK NGKKTAVVKE 100
EEDFNEIAKP SPKHKRVSKA NGSKNGAKSA VKKEESDTDD SVPLRAVSTV 150
SLTPYKSELP SGASTTQNRS PNDEEDEDED YKWWTSENID DTQKWTTLEH 200
NGVIFAPPYE PLPKNVKLIY DGNPVNLPPE AEEVAGFYAA MLETDHAKNP 250
VFQDNFFRDF LKVCDECNFN HNIKEFSKCD FTQMFHHFEQ KREEKKSMPK 300
EQKKAIKQKK DEEEEKYKWC ILDGRKEKVG NFRIEPPGLF RGRGSHPKTG 350
SLKRRVYPEQ ITINIGEGVP VPEPLPGHQW AEVKHDNTVT WLATWHENIN 400
NNVKYVFLAA GSSLKGQSDL KKYEKSRKLK DYIDDIRKGY RKDLKSELTV 450
ERQRGTAMYL IDVFALRAGN EKGEDEADTV GCCSLRYEHV TLKPPRTVVF 500
DFLGKDSIRY YNEVEVDPQV FKNLKIFKRP PKKEGDLIFD RLSTNSLNKY 550
LTSLMDGLSA KVFRTYNASY TMAEELKKMP KNLTLADKIL FYNRANRTVA 600
ILCNHQRSVT KNHDVQMERF AERIKALQYQ RMRLRKMMLN LEPKLAKSKP 650
ELLAKEEGIT DSWIVKHHET LYELEKEKIK KKFDRENEKL AAEDPKSVLP 700
ESELEVRLKA ADELKKALDA ELKSKKVDPG RSSMEQLEKR LNKLNERINV 750
MRTQMIDKDE NKTTALGTSK INYIDPRLTY SFSKREDVPI EKLFSKTIRD 800
KFNWAADTPP DWKW 814
Length:814
Mass (Da):93,981
Last modified:April 27, 2001 - v2
Checksum:i842D8D81C92CB0A3
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 82Missing1 Publication
Sequence conflicti308 – 3081Q → E in CAA29559. 1 Publication
Sequence conflicti446 – 4461S → N in CAA29559. 1 Publication
Sequence conflicti698 – 6981V → M in CAA29559. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06201 Genomic DNA. Translation: CAA29559.1.
CU329671 Genomic DNA. Translation: CAB66458.1.
PIRiS03329. ISZPT1.
T50327.
RefSeqiNP_596209.1. NM_001022128.2.

Genome annotation databases

EnsemblFungiiSPBC1703.14c.1; SPBC1703.14c.1:pep; SPBC1703.14c.
GeneIDi2540032.
KEGGispo:SPBC1703.14c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06201 Genomic DNA. Translation: CAA29559.1 .
CU329671 Genomic DNA. Translation: CAB66458.1 .
PIRi S03329. ISZPT1.
T50327.
RefSeqi NP_596209.1. NM_001022128.2.

3D structure databases

ProteinModelPortali P07799.
SMRi P07799. Positions 180-608, 762-807.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 276575. 81 interactions.
MINTi MINT-4686963.
STRINGi 4896.SPBC1703.14c-1.

Proteomic databases

MaxQBi P07799.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC1703.14c.1 ; SPBC1703.14c.1:pep ; SPBC1703.14c .
GeneIDi 2540032.
KEGGi spo:SPBC1703.14c.

Organism-specific databases

PomBasei SPBC1703.14c.

Phylogenomic databases

eggNOGi COG3569.
HOGENOMi HOG000105469.
KOi K03163.
OMAi KSVDEDW.
OrthoDBi EOG7966R0.
PhylomeDBi P07799.

Miscellaneous databases

NextBioi 20801174.

Family and domain databases

Gene3Di 1.10.10.41. 1 hit.
1.10.132.10. 2 hits.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProi IPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view ]
Pfami PF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view ]
PRINTSi PR00416. EUTPISMRASEI.
SMARTi SM00435. TOPEUc. 1 hit.
[Graphical view ]
SUPFAMi SSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of Schizosaccharomyces pombe DNA topoisomerase I gene, and effect of gene disruption."
    Uemura T., Morino K., Uzawa S., Shiozaki K., Yanagida M.
    Nucleic Acids Res. 15:9727-9739(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Mapping of the active site tyrosine of eukaryotic DNA topoisomerase I."
    Eng W.-K., Pandit S.D., Sternglanz R.
    J. Biol. Chem. 264:13373-13376(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITE TYR-773.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; SER-54; SER-136 AND THR-138, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiTOP1_SCHPO
AccessioniPrimary (citable) accession number: P07799
Secondary accession number(s): Q9P7V7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: April 27, 2001
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi