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Protein

DNA topoisomerase 1

Gene

top1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. TThe free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei773O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

  • DNA binding Source: PomBase
  • DNA topoisomerase type I activity Source: PomBase

GO - Biological processi

  • chromatin assembly or disassembly Source: PomBase
  • chromatin organization Source: PomBase
  • chromatin remodeling Source: GO_Central
  • chromosome segregation Source: GO_Central
  • DNA replication Source: GO_Central
  • DNA topological change Source: PomBase
  • mitotic chromosome condensation Source: PomBase

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase

Enzyme and pathway databases

ReactomeiR-SPO-4615885 SUMOylation of DNA replication proteins

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Gene namesi
Name:top1
ORF Names:SPBC1703.14c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC1703.14c
PomBaseiSPBC1703.14c top1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001452091 – 814DNA topoisomerase 1Add BLAST814

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52Phosphoserine1 Publication1
Modified residuei54Phosphoserine1 Publication1
Modified residuei136Phosphoserine1 Publication1
Modified residuei138Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07799
PaxDbiP07799
PRIDEiP07799

PTM databases

iPTMnetiP07799
SwissPalmiP07799

Interactioni

Subunit structurei

Monomer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei343Interaction with DNABy similarity1
Sitei391Interaction with DNABy similarity1
Sitei422Interaction with DNABy similarity1
Sitei479Interaction with DNABy similarity1
Sitei505Interaction with DNABy similarity1
Sitei548Interaction with DNABy similarity1
Sitei605Interaction with DNABy similarity1

Protein-protein interaction databases

BioGridi276575, 85 interactors
STRINGi4896.SPBC1703.14c.1

Structurei

3D structure databases

ProteinModelPortaliP07799
SMRiP07799
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni404 – 405Interaction with DNABy similarity2
Regioni467 – 472Interaction with DNABy similarity6
Regioni559 – 561Interaction with DNABy similarity3

Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Phylogenomic databases

HOGENOMiHOG000105469
InParanoidiP07799
KOiK03163
OMAiATWLAMW
OrthoDBiEOG092C0N3X
PhylomeDBiP07799

Family and domain databases

CDDicd00659 Topo_IB_C, 1 hit
Gene3Di1.10.10.41, 1 hit
1.10.132.10, 1 hit
2.170.11.10, 2 hits
3.90.15.10, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013034 DNA_topo_DNA_db_N_dom1
IPR013030 DNA_topo_DNA_db_N_dom2
IPR001631 TopoI
IPR018521 TopoI_AS
IPR025834 TopoI_C_dom
IPR014711 TopoI_cat_a-hlx-sub_euk
IPR014727 TopoI_cat_a/b-sub_euk
IPR013500 TopoI_cat_euk
IPR008336 TopoI_DNA-bd_euk
IPR036202 TopoI_DNA-bd_euk_N_sf
IPR013499 TopoI_euk
PANTHERiPTHR10290 PTHR10290, 1 hit
PfamiView protein in Pfam
PF14370 Topo_C_assoc, 1 hit
PF01028 Topoisom_I, 1 hit
PF02919 Topoisom_I_N, 1 hit
PRINTSiPR00416 EUTPISMRASEI
SMARTiView protein in SMART
SM00435 TOPEUc, 1 hit
SUPFAMiSSF56349 SSF56349, 2 hits
SSF56741 SSF56741, 1 hit
PROSITEiView protein in PROSITE
PS00176 TOPOISOMERASE_I_EUK, 1 hit

Sequencei

Sequence statusi: Complete.

P07799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSDSDSVS LSIRRRQRRG SSKRISMKES DEESDSSENH PLSESLNKKS
60 70 80 90 100
KSESDEDDIP IRKRRASSKK NMSNSSSKKR AKVMGNGGLK NGKKTAVVKE
110 120 130 140 150
EEDFNEIAKP SPKHKRVSKA NGSKNGAKSA VKKEESDTDD SVPLRAVSTV
160 170 180 190 200
SLTPYKSELP SGASTTQNRS PNDEEDEDED YKWWTSENID DTQKWTTLEH
210 220 230 240 250
NGVIFAPPYE PLPKNVKLIY DGNPVNLPPE AEEVAGFYAA MLETDHAKNP
260 270 280 290 300
VFQDNFFRDF LKVCDECNFN HNIKEFSKCD FTQMFHHFEQ KREEKKSMPK
310 320 330 340 350
EQKKAIKQKK DEEEEKYKWC ILDGRKEKVG NFRIEPPGLF RGRGSHPKTG
360 370 380 390 400
SLKRRVYPEQ ITINIGEGVP VPEPLPGHQW AEVKHDNTVT WLATWHENIN
410 420 430 440 450
NNVKYVFLAA GSSLKGQSDL KKYEKSRKLK DYIDDIRKGY RKDLKSELTV
460 470 480 490 500
ERQRGTAMYL IDVFALRAGN EKGEDEADTV GCCSLRYEHV TLKPPRTVVF
510 520 530 540 550
DFLGKDSIRY YNEVEVDPQV FKNLKIFKRP PKKEGDLIFD RLSTNSLNKY
560 570 580 590 600
LTSLMDGLSA KVFRTYNASY TMAEELKKMP KNLTLADKIL FYNRANRTVA
610 620 630 640 650
ILCNHQRSVT KNHDVQMERF AERIKALQYQ RMRLRKMMLN LEPKLAKSKP
660 670 680 690 700
ELLAKEEGIT DSWIVKHHET LYELEKEKIK KKFDRENEKL AAEDPKSVLP
710 720 730 740 750
ESELEVRLKA ADELKKALDA ELKSKKVDPG RSSMEQLEKR LNKLNERINV
760 770 780 790 800
MRTQMIDKDE NKTTALGTSK INYIDPRLTY SFSKREDVPI EKLFSKTIRD
810
KFNWAADTPP DWKW
Length:814
Mass (Da):93,981
Last modified:April 27, 2001 - v2
Checksum:i842D8D81C92CB0A3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7 – 8Missing (PubMed:2827111).Curated2
Sequence conflicti308Q → E in CAA29559 (PubMed:2827111).Curated1
Sequence conflicti446S → N in CAA29559 (PubMed:2827111).Curated1
Sequence conflicti698V → M in CAA29559 (PubMed:2827111).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06201 Genomic DNA Translation: CAA29559.1
CU329671 Genomic DNA Translation: CAB66458.1
PIRiS03329 ISZPT1
T50327
RefSeqiNP_596209.1, NM_001022128.2

Genome annotation databases

EnsemblFungiiSPBC1703.14c.1; SPBC1703.14c.1:pep; SPBC1703.14c
GeneIDi2540032
KEGGispo:SPBC1703.14c

Similar proteinsi

Entry informationi

Entry nameiTOP1_SCHPO
AccessioniPrimary (citable) accession number: P07799
Secondary accession number(s): Q9P7V7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: April 27, 2001
Last modified: May 23, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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