Reviewed, UniProtKB/Swiss-Prot P07799 (TOP1_SCHPO)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I

Gene names

Name:	top1
ORF Names:	SPBC1703.14c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	814 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer.
Miscellaneous	Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils. When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the eukaryotic type I topoisomerase family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from mutant phenotype. Source: GeneDB_SPombe DNA unwinding during replication Inferred from electronic annotation. Source: InterPro chromatin organization Inferred from genetic interaction. Source: GeneDB_SPombe transcription of nuclear rRNA large RNA polymerase I transcript Inferred from genetic interaction. Source: GeneDB_SPombe
Cellular component	nuclear chromatin Non-traceable author statement. Source: GeneDB_SPombe nucleolus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 814	814	DNA topoisomerase 1		PRO_0000145209
Sites
	Active site	773	1	O-(3'-phospho-DNA)-tyrosine intermediate Ref.3
Amino acid modifications
	Modified residue	52	1	Phosphoserine Ref.4
	Modified residue	54	1	Phosphoserine Ref.4
	Modified residue	136	1	Phosphoserine Ref.4
	Modified residue	138	1	Phosphothreonine Ref.4
Experimental info
	Sequence conflict	7 – 8	2	Missing Ref.1
	Sequence conflict	308	1	Q → E in CAA29559. Ref.1
	Sequence conflict	446	1	S → N in CAA29559. Ref.1
	Sequence conflict	698	1	V → M in CAA29559. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07799-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 842D8D81C92CB0A3	FASTA	814	93,981
	10 20 30 40 50 60 MSSSDSDSVS LSIRRRQRRG SSKRISMKES DEESDSSENH PLSESLNKKS KSESDEDDIP 70 80 90 100 110 120 IRKRRASSKK NMSNSSSKKR AKVMGNGGLK NGKKTAVVKE EEDFNEIAKP SPKHKRVSKA 130 140 150 160 170 180 NGSKNGAKSA VKKEESDTDD SVPLRAVSTV SLTPYKSELP SGASTTQNRS PNDEEDEDED 190 200 210 220 230 240 YKWWTSENID DTQKWTTLEH NGVIFAPPYE PLPKNVKLIY DGNPVNLPPE AEEVAGFYAA 250 260 270 280 290 300 MLETDHAKNP VFQDNFFRDF LKVCDECNFN HNIKEFSKCD FTQMFHHFEQ KREEKKSMPK 310 320 330 340 350 360 EQKKAIKQKK DEEEEKYKWC ILDGRKEKVG NFRIEPPGLF RGRGSHPKTG SLKRRVYPEQ 370 380 390 400 410 420 ITINIGEGVP VPEPLPGHQW AEVKHDNTVT WLATWHENIN NNVKYVFLAA GSSLKGQSDL 430 440 450 460 470 480 KKYEKSRKLK DYIDDIRKGY RKDLKSELTV ERQRGTAMYL IDVFALRAGN EKGEDEADTV 490 500 510 520 530 540 GCCSLRYEHV TLKPPRTVVF DFLGKDSIRY YNEVEVDPQV FKNLKIFKRP PKKEGDLIFD 550 560 570 580 590 600 RLSTNSLNKY LTSLMDGLSA KVFRTYNASY TMAEELKKMP KNLTLADKIL FYNRANRTVA 610 620 630 640 650 660 ILCNHQRSVT KNHDVQMERF AERIKALQYQ RMRLRKMMLN LEPKLAKSKP ELLAKEEGIT 670 680 690 700 710 720 DSWIVKHHET LYELEKEKIK KKFDRENEKL AAEDPKSVLP ESELEVRLKA ADELKKALDA 730 740 750 760 770 780 ELKSKKVDPG RSSMEQLEKR LNKLNERINV MRTQMIDKDE NKTTALGTSK INYIDPRLTY 790 800 810 SFSKREDVPI EKLFSKTIRD KFNWAADTPP DWKW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of Schizosaccharomyces pombe DNA topoisomerase I gene, and effect of gene disruption." Uemura T., Morino K., Uzawa S., Shiozaki K., Yanagida M. Nucleic Acids Res. 15:9727-9739(1987) [PubMed: 2827111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Mapping of the active site tyrosine of eukaryotic DNA topoisomerase I." Eng W.-K., Pandit S.D., Sternglanz R. J. Biol. Chem. 264:13373-13376(1989) [PubMed: 2547758] [Abstract] Cited for: ACTIVE SITE TYR-773.
[4]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; SER-54; SER-136 AND THR-138, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X06201 Genomic DNA. Translation: CAA29559.1. CU329671 Genomic DNA. Translation: CAB66458.1.
PIR	ISZPT1. S03329. T50327.
RefSeq	NP_596209.1.
3D structure databases
HSSP	HSSP built from PDB template 1OIS based on UniProtKB P04786.
ModBase	Search...
Protein-protein interaction databases
STRING	P07799.
Genome annotation databases
GeneID	2540032.
GenomeReviews	Gene locus top1 in contig CU329671_GR.
KEGG	spo:SPBC1703.14c.
NMPDR	fig|4896.1.peg.2075.
Organism-specific databases
GeneDB_Spombe	SPBC1703.14c.
Phylogenomic databases
OMA	GYCLLDH.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004239-MON.
BRENDA	5.99.1.2. 653.
Gene expression databases
ArrayExpress	P07799.
Family and domain databases
InterPro	IPR018521. TopoI_AS. IPR001631. TopoI_C. IPR013499. TopoI_C_euk. IPR014711. TopoI_cat_a-hlx-sub_euk. IPR014727. TopoI_cat_a/b-sub_euk. IPR013500. TopoI_cat_euk. IPR013030. TopoI_DNA-bd_mixed-a/b_euk. IPR008336. TopoI_DNA_bd_euk. [Graphical view]
Gene3D	G3DSA:3.90.15.10. TopoI_cat_a-hlx-sub_euk. 1 hit. G3DSA:1.10.132.10. TopoI_cat_a/b-sub_euk. 1 hit. G3DSA:2.170.11.10. TopoI_DNA-bd_mixed-a/b_euk. 1 hit.
Pfam	PF01028. Topoisom_I. 1 hit. PF02919. Topoisom_I_N. 1 hit. [Graphical view]
PRINTS	PR00416. EUTPISMRASEI.
SMART	SM00435. TOPEUc. 1 hit. [Graphical view]
PROSITE	PS00176. TOPOISOMERASE_I_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_SCHPO

Accession

Primary (citable) accession number: P07799
Secondary accession number(s): Q9P7V7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	April 27, 2001
Last modified:	November 3, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)