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P07799 (TOP1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Gene names
Name:top1
ORF Names:SPBC1703.14c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length814 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Subunit structure

Monomer.

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Sequence similarities

Belongs to the type IB topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 814814DNA topoisomerase 1
PRO_0000145209

Regions

Region404 – 4052Interaction with DNA By similarity
Region467 – 4726Interaction with DNA By similarity
Region559 – 5613Interaction with DNA By similarity

Sites

Active site7731O-(3'-phospho-DNA)-tyrosine intermediate Ref.3
Site3431Interaction with DNA By similarity
Site3911Interaction with DNA By similarity
Site4221Interaction with DNA By similarity
Site4791Interaction with DNA By similarity
Site5051Interaction with DNA By similarity
Site5481Interaction with DNA By similarity
Site6051Interaction with DNA By similarity

Amino acid modifications

Modified residue521Phosphoserine Ref.4
Modified residue541Phosphoserine Ref.4
Modified residue1361Phosphoserine Ref.4
Modified residue1381Phosphothreonine Ref.4

Experimental info

Sequence conflict7 – 82Missing Ref.1
Sequence conflict3081Q → E in CAA29559. Ref.1
Sequence conflict4461S → N in CAA29559. Ref.1
Sequence conflict6981V → M in CAA29559. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P07799 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 842D8D81C92CB0A3

FASTA81493,981
        10         20         30         40         50         60 
MSSSDSDSVS LSIRRRQRRG SSKRISMKES DEESDSSENH PLSESLNKKS KSESDEDDIP 

        70         80         90        100        110        120 
IRKRRASSKK NMSNSSSKKR AKVMGNGGLK NGKKTAVVKE EEDFNEIAKP SPKHKRVSKA 

       130        140        150        160        170        180 
NGSKNGAKSA VKKEESDTDD SVPLRAVSTV SLTPYKSELP SGASTTQNRS PNDEEDEDED 

       190        200        210        220        230        240 
YKWWTSENID DTQKWTTLEH NGVIFAPPYE PLPKNVKLIY DGNPVNLPPE AEEVAGFYAA 

       250        260        270        280        290        300 
MLETDHAKNP VFQDNFFRDF LKVCDECNFN HNIKEFSKCD FTQMFHHFEQ KREEKKSMPK 

       310        320        330        340        350        360 
EQKKAIKQKK DEEEEKYKWC ILDGRKEKVG NFRIEPPGLF RGRGSHPKTG SLKRRVYPEQ 

       370        380        390        400        410        420 
ITINIGEGVP VPEPLPGHQW AEVKHDNTVT WLATWHENIN NNVKYVFLAA GSSLKGQSDL 

       430        440        450        460        470        480 
KKYEKSRKLK DYIDDIRKGY RKDLKSELTV ERQRGTAMYL IDVFALRAGN EKGEDEADTV 

       490        500        510        520        530        540 
GCCSLRYEHV TLKPPRTVVF DFLGKDSIRY YNEVEVDPQV FKNLKIFKRP PKKEGDLIFD 

       550        560        570        580        590        600 
RLSTNSLNKY LTSLMDGLSA KVFRTYNASY TMAEELKKMP KNLTLADKIL FYNRANRTVA 

       610        620        630        640        650        660 
ILCNHQRSVT KNHDVQMERF AERIKALQYQ RMRLRKMMLN LEPKLAKSKP ELLAKEEGIT 

       670        680        690        700        710        720 
DSWIVKHHET LYELEKEKIK KKFDRENEKL AAEDPKSVLP ESELEVRLKA ADELKKALDA 

       730        740        750        760        770        780 
ELKSKKVDPG RSSMEQLEKR LNKLNERINV MRTQMIDKDE NKTTALGTSK INYIDPRLTY 

       790        800        810 
SFSKREDVPI EKLFSKTIRD KFNWAADTPP DWKW 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of Schizosaccharomyces pombe DNA topoisomerase I gene, and effect of gene disruption."
Uemura T., Morino K., Uzawa S., Shiozaki K., Yanagida M.
Nucleic Acids Res. 15:9727-9739(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]"Mapping of the active site tyrosine of eukaryotic DNA topoisomerase I."
Eng W.-K., Pandit S.D., Sternglanz R.
J. Biol. Chem. 264:13373-13376(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: ACTIVE SITE TYR-773.
[4]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; SER-54; SER-136 AND THR-138, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06201 Genomic DNA. Translation: CAA29559.1.
CU329671 Genomic DNA. Translation: CAB66458.1.
PIRISZPT1. S03329.
T50327.
RefSeqNP_596209.1. NM_001022128.2.

3D structure databases

ProteinModelPortalP07799.
SMRP07799. Positions 180-608, 762-807.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid276575. 81 interactions.
MINTMINT-4686963.
STRING4896.SPBC1703.14c-1.

Proteomic databases

MaxQBP07799.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC1703.14c.1; SPBC1703.14c.1:pep; SPBC1703.14c.
GeneID2540032.
KEGGspo:SPBC1703.14c.

Organism-specific databases

PomBaseSPBC1703.14c.

Phylogenomic databases

eggNOGCOG3569.
HOGENOMHOG000105469.
KOK03163.
OMAKSVDEDW.
OrthoDBEOG7966R0.
PhylomeDBP07799.

Family and domain databases

Gene3D1.10.10.41. 1 hit.
1.10.132.10. 2 hits.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SMARTSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20801174.

Entry information

Entry nameTOP1_SCHPO
AccessionPrimary (citable) accession number: P07799
Secondary accession number(s): Q9P7V7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: April 27, 2001
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names