Reviewed, UniProtKB/Swiss-Prot P07772 (BEND_ACIAD)

Names and origin

Protein names

Recommended name:
    1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase
    EC=1.3.1.25
Alternative name(s):
    Cis-1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase
    Cis-1,2-dihydroxy-3,4-cyclohexadiene-1-carboxylate dehydrogenase
    2-hydro-1,2-dihydroxybenzoate dehydrogenase
      Short name=DHB dehydrogenase

Gene names

Name:	benD
Ordered Locus Names:	ACIAD1439

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degradation of 2-hydro-1,2-dihydroxy benzoate (DHB) to catechol.
Catalytic activity	(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH.
Pathway	Aromatic compound metabolism; benzoic acid degradation via hydroxylation; catechol from benzoic acid: step 2/2.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase		PRO_0000054529
Regions
	Nucleotide binding	13 – 37	25	NAD By similarity
Sites
	Active site	156	1	Proton acceptor By similarity
	Binding site	145	1	Substrate By similarity
Experimental info
	Sequence conflict	159	1	S → C in AAC46439. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07772-1 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: E29D0FAC6E9AE55E	FASTA	261	27,982
	10 20 30 40 50 60 MNSTQRFEHK VVIVTGAAQG IGRGVALRIA QEGGCLILAD RSDLIQAVLA EIKALGALAI 70 80 90 100 110 120 AVETDLETYA GAELVVSHAI AEYGRIDVLI NNVGGAIWMK PFQEFSEEEI IQEVHRSLFP 130 140 150 160 170 180 ALWCCRAVLP EMLKHQQGTI VNVSSIATRG IHRIPYSASK GGVNALTASL AFEHAQHGIR 190 200 210 220 230 240 VNAVATGGTK APPRKIPRNA QPLSKSEQVW MQQVVDQTID RSFLGRYGSI DEQVNAITFL 250 260 ASDESSYITG SVLPVGGGDQ G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily." Neidle E.L., Hartnett C., Ornston L.N., Bairoch A., Rekik M., Harayama S. Eur. J. Biochem. 204:113-120(1992) [PubMed: 1740120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF009224 Genomic DNA. Translation: AAC46439.1. CR543861 Genomic DNA. Translation: CAG68303.1.
PIR	S23480.
RefSeq	YP_046125.1.
3D structure databases
HSSP	HSSP built from PDB template 1GZ6 based on UniProtKB P97852.
ModBase	Search...
Genome annotation databases
GeneID	2880309.
GenomeReviews	Gene locus ACIAD1439 in contig CR543861_GR.
KEGG	aci:ACIAD1439.
NMPDR	fig|62977.3.peg.797.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P07772.
OMA	P07772. MQQVVDQ.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD1439-MON.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BEND_ACIAD

Accession

Primary (citable) accession number: P07772
Secondary accession number(s): Q6FCA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	August 31, 2004
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)