ID   BENB_ACIAD              Reviewed;         169 AA.
AC   P07770;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   16-JUN-2009, entry version 61.
DE   RecName: Full=Benzoate 1,2-dioxygenase subunit beta;
DE            EC=1.14.12.10;
GN   Name=benB; OrderedLocusNames=ACIAD1437;
OS   Acinetobacter sp. (strain ADP1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Moraxellaceae; Acinetobacter.
OX   NCBI_TaxID=62977;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91358314; PubMed=1885518;
RA   Neidle E.L., Hartnett C., Ornston N.L., Bairoch A., Rekik M.,
RA   Harayama S.;
RT   "Nucleotide sequences of the Acinetobacter calcoaceticus benABC genes
RT   for benzoate 1,2-dioxygenase reveal evolutionary relationships among
RT   multicomponent oxygenases.";
RL   J. Bacteriol. 173:5385-5395(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15514110; DOI=10.1093/nar/gkh910;
RA   Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S.,
RA   Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P.,
RA   Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.;
RT   "Unique features revealed by the genome sequence of Acinetobacter sp.
RT   ADP1, a versatile and naturally transformation competent bacterium.";
RL   Nucleic Acids Res. 32:5766-5779(2004).
CC   -!- FUNCTION: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate
CC       (DHB). The beta subunit may be responsible for the substrate
CC       specificity of the enzyme.
CC   -!- CATALYTIC ACTIVITY: Benzoate + NADH + O(2) = (1R,6S)-1,6-
CC       dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD(+).
CC   -!- PATHWAY: Aromatic compound metabolism; benzoic acid degradation
CC       via hydroxylation; catechol from benzoic acid: step 1/2.
CC   -!- SUBUNIT: This dioxygenase system consists of three proteins: the
CC       two subunits of the hydroxylase (benA and benB), and an electron
CC       transfer component (benC).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase beta subunit family.
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DR   EMBL; AF009224; AAC46437.1; -; Genomic_DNA.
DR   EMBL; CR543861; CAG68301.1; -; Genomic_DNA.
DR   PIR; S23478; S23478.
DR   RefSeq; YP_046123.1; -.
DR   GeneID; 2880891; -.
DR   GenomeReviews; CR543861_GR; ACIAD1437.
DR   KEGG; aci:ACIAD1437; -.
DR   NMPDR; fig|62977.3.peg.795; -.
DR   HOGENOM; P07770; -.
DR   OMA; P07770; TLSFRYK.
DR   BioCyc; ASP62977:ACIAD1437-MON; -.
DR   GO; GO:0018623; F:benzoate 1,2-dioxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017641; Benzo_1-2-diOase_ssu.
DR   InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR   Pfam; PF00866; Ring_hydroxyl_B; 1.
DR   TIGRFAMs; TIGR03232; benzo_1_2_benB; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Complete proteome; Dioxygenase; NAD;
KW   Oxidoreductase.
FT   CHAIN         1    169       Benzoate 1,2-dioxygenase subunit beta.
FT                                /FTId=PRO_0000085065.
SQ   SEQUENCE   169 AA;  20073 MW;  4B0CD8F8AA001325 CRC64;
     MNATALLDTI SIEQISQFLY SEARFLDDEQ WDDWLECYAP QASFWMPAWD DNDQLTENPQ
     TEISLIYYPD RQGLEDRVFR IKTERSSATM PDTRTAHNIS NIEVESRDGL QITVRFNWNT
     LSFRYKNSYS YFGMSRYVID FSGEQPKILS KYVMLKNDYI NQVIDIYHI
//