Reviewed, UniProtKB/Swiss-Prot P07770 (BENB_ACIAD)

Names and origin

Protein names

Recommended name:
    Benzoate 1,2-dioxygenase subunit beta
    EC=1.14.12.10

Gene names

Name:	benB
Ordered Locus Names:	ACIAD1437

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB). The beta subunit may be responsible for the substrate specificity of the enzyme.
Catalytic activity	Benzoate + NADH + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+.
Pathway	Aromatic compound metabolism; benzoic acid degradation via hydroxylation; catechol from benzoic acid: step 1/2.
Subunit structure	This dioxygenase system consists of three proteins: the two subunits of the hydroxylase (benA and benB), and an electron transfer component (benC).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	benzoate 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Benzoate 1,2-dioxygenase subunit beta		PRO_0000085065

Sequences

	Sequence		Length	Mass (Da)
	P07770-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 4B0CD8F8AA001325	FASTA	169	20,073
	10 20 30 40 50 60 MNATALLDTI SIEQISQFLY SEARFLDDEQ WDDWLECYAP QASFWMPAWD DNDQLTENPQ 70 80 90 100 110 120 TEISLIYYPD RQGLEDRVFR IKTERSSATM PDTRTAHNIS NIEVESRDGL QITVRFNWNT 130 140 150 160 LSFRYKNSYS YFGMSRYVID FSGEQPKILS KYVMLKNDYI NQVIDIYHI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequences of the Acinetobacter calcoaceticus benABC genes for benzoate 1,2-dioxygenase reveal evolutionary relationships among multicomponent oxygenases." Neidle E.L., Hartnett C., Ornston N.L., Bairoch A., Rekik M., Harayama S. J. Bacteriol. 173:5385-5395(1991) [PubMed: 1885518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF009224 Genomic DNA. Translation: AAC46437.1. CR543861 Genomic DNA. Translation: CAG68301.1.
PIR	S23478.
RefSeq	YP_046123.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2880891.
GenomeReviews	Gene locus ACIAD1437 in contig CR543861_GR.
KEGG	aci:ACIAD1437.
NMPDR	fig|62977.3.peg.795.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P07770.
OMA	P07770. TLSFRYK.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD1437-MON.
Family and domain databases
InterPro	IPR017641. Benzo_1-2-diOase_ssu. IPR000391. Rng_hydr_dOase-bsu. [Graphical view]
Pfam	PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03232. benzo_1_2_benB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BENB_ACIAD

Accession

Primary (citable) accession number: P07770

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)