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P07762

- GLGB_ECOLI

UniProt

P07762 - GLGB_ECOLI

Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei405 – 4051NucleophileBy similarity
    Active sitei458 – 4581Proton donorBy similarity

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: EcoCyc
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: EcoliWiki
    2. glycogen biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    BioCyciEcoCyc:GLYCOGEN-BRANCH-MONOMER.
    ECOL316407:JW3395-MONOMER.
    MetaCyc:GLYCOGEN-BRANCH-MONOMER.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
    Alpha-(1->4)-glucan branching enzyme
    Glycogen branching enzyme
    Short name:
    BE
    Gene namesi
    Name:glgB
    Ordered Locus Names:b3432, JW3395
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10378. glgB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7287281,4-alpha-glucan branching enzyme GlgBPRO_0000188702Add
    BLAST

    Proteomic databases

    PaxDbiP07762.
    PRIDEiP07762.

    Expressioni

    Gene expression databases

    GenevestigatoriP07762.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    IntActiP07762. 13 interactions.
    STRINGi511145.b3432.

    Structurei

    Secondary structure

    1
    728
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi121 – 1233
    Beta strandi125 – 13410
    Beta strandi136 – 1438
    Beta strandi150 – 1545
    Helixi155 – 1573
    Turni161 – 1633
    Turni170 – 1723
    Beta strandi174 – 1807
    Beta strandi187 – 1937
    Beta strandi199 – 2024
    Beta strandi208 – 2114
    Turni213 – 2153
    Beta strandi217 – 2193
    Helixi231 – 2377
    Beta strandi244 – 2485
    Turni257 – 2593
    Helixi265 – 27814
    Beta strandi282 – 2876
    Helixi295 – 2973
    Beta strandi303 – 3086
    Helixi310 – 3123
    Helixi315 – 32713
    Beta strandi331 – 3366
    Helixi351 – 3533
    Beta strandi356 – 3583
    Helixi380 – 39617
    Beta strandi401 – 4044
    Helixi408 – 4114
    Helixi433 – 44816
    Beta strandi454 – 4574
    Turni464 – 4674
    Turni470 – 4734
    Beta strandi478 – 4814
    Helixi483 – 49412
    Helixi497 – 5026
    Helixi505 – 5084
    Helixi509 – 5113
    Turni512 – 5165
    Beta strandi519 – 5235
    Helixi525 – 5273
    Helixi535 – 5384
    Helixi543 – 55917
    Beta strandi560 – 5678
    Turni568 – 5736
    Helixi586 – 5894
    Helixi595 – 61016
    Helixi614 – 6163
    Helixi623 – 6253
    Beta strandi626 – 6338
    Turni634 – 6374
    Beta strandi638 – 6447
    Beta strandi650 – 6567
    Beta strandi662 – 6654
    Beta strandi673 – 6808
    Helixi685 – 6873
    Beta strandi713 – 7153
    Beta strandi719 – 7268

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GTYmodel-A24-103[»]
    1M7XX-ray2.30A/B/C/D113-728[»]
    3O7YX-ray2.41A/B/C/D117-728[»]
    3O7ZX-ray2.55A/B/C/D117-728[»]
    ProteinModelPortaliP07762.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07762.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0296.
    HOGENOMiHOG000283037.
    KOiK00700.
    OMAiAKLLFMG.
    OrthoDBiEOG6JX7GT.
    PhylomeDBiP07762.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P07762-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDRIDRDVI NALIAGHFAD PFSVLGMHKT TAGLEVRALL PDATDVWVIE    50
    PKTGRKLAKL ECLDSRGFFS GVIPRRKNFF RYQLAVVWHG QQNLIDDPYR 100
    FGPLIQEMDA WLLSEGTHLR PYETLGAHAD TMDGVTGTRF SVWAPNARRV 150
    SVVGQFNYWD GRRHPMRLRK ESGIWELFIP GAHNGQLYKY EMIDANGNLR 200
    LKSDPYAFEA QMRPETASLI CGLPEKVVQT EERKKANQFD APISIYEVHL 250
    GSWRRHTDNN FWLSYRELAD QLVPYAKWMG FTHLELLPIN EHPFDGSWGY 300
    QPTGLYAPTR RFGTRDDFRY FIDAAHAAGL NVILDWVPGH FPTDDFALAE 350
    FDGTNLYEHS DPREGYHQDW NTLIYNYGRR EVSNFLVGNA LYWIERFGID 400
    ALRVDAVASM IYRDYSRKEG EWIPNEFGGR ENLEAIEFLR NTNRILGEQV 450
    SGAVTMAEES TDFPGVSRPQ DMGGLGFWYK WNLGWMHDTL DYMKLDPVYR 500
    QYHHDKLTFG ILYNYTENFV LPLSHDEVVH GKKSILDRMP GDAWQKFANL 550
    RAYYGWMWAF PGKKLLFMGN EFAQGREWNH DASLDWHLLE GGDNWHHGVQ 600
    RLVRDLNLTY RHHKAMHELD FDPYGFEWLV VDDKERSVLI FVRRDKEGNE 650
    IIVASNFTPV PRHDYRFGIN QPGKWREILN TDSMHYHGSN AGNGGTVHSD 700
    EIASHGRQHS LSLTLPPLAT IWLVREAE 728
    Length:728
    Mass (Da):84,337
    Last modified:August 1, 1988 - v1
    Checksum:i0F20AF3677BF2015
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13751 Genomic DNA. Translation: AAA23872.1.
    U18997 Genomic DNA. Translation: AAA58230.1.
    U00096 Genomic DNA. Translation: AAC76457.1.
    AP009048 Genomic DNA. Translation: BAE77860.1.
    PIRiA25498. NQECA.
    RefSeqiNP_417890.1. NC_000913.3.
    YP_492001.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76457; AAC76457; b3432.
    BAE77860; BAE77860; BAE77860.
    GeneIDi12933509.
    947940.
    KEGGiecj:Y75_p3745.
    eco:b3432.
    PATRICi32122304. VBIEscCol129921_3529.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13751 Genomic DNA. Translation: AAA23872.1 .
    U18997 Genomic DNA. Translation: AAA58230.1 .
    U00096 Genomic DNA. Translation: AAC76457.1 .
    AP009048 Genomic DNA. Translation: BAE77860.1 .
    PIRi A25498. NQECA.
    RefSeqi NP_417890.1. NC_000913.3.
    YP_492001.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GTY model - A 24-103 [» ]
    1M7X X-ray 2.30 A/B/C/D 113-728 [» ]
    3O7Y X-ray 2.41 A/B/C/D 117-728 [» ]
    3O7Z X-ray 2.55 A/B/C/D 117-728 [» ]
    ProteinModelPortali P07762.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P07762. 13 interactions.
    STRINGi 511145.b3432.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PaxDbi P07762.
    PRIDEi P07762.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76457 ; AAC76457 ; b3432 .
    BAE77860 ; BAE77860 ; BAE77860 .
    GeneIDi 12933509.
    947940.
    KEGGi ecj:Y75_p3745.
    eco:b3432.
    PATRICi 32122304. VBIEscCol129921_3529.

    Organism-specific databases

    EchoBASEi EB0373.
    EcoGenei EG10378. glgB.

    Phylogenomic databases

    eggNOGi COG0296.
    HOGENOMi HOG000283037.
    KOi K00700.
    OMAi AKLLFMG.
    OrthoDBi EOG6JX7GT.
    PhylomeDBi P07762.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci EcoCyc:GLYCOGEN-BRANCH-MONOMER.
    ECOL316407:JW3395-MONOMER.
    MetaCyc:GLYCOGEN-BRANCH-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P07762.
    PROi P07762.

    Gene expression databases

    Genevestigatori P07762.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-alpha-D-(1,4-alpha-D-glucano)-transferase as deduced from the nucleotide sequence of the glg B gene."
      Baecker P.A., Greenberg E., Preiss J.
      J. Biol. Chem. 261:8738-8743(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "The X-ray crystallographic structure of Escherichia coli branching enzyme."
      Abad M.C., Binderup K., Rios-Steiner J., Arni R.K., Preiss J., Geiger J.H.
      J. Biol. Chem. 277:42164-42170(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 113-728.

    Entry informationi

    Entry nameiGLGB_ECOLI
    AccessioniPrimary (citable) accession number: P07762
    Secondary accession number(s): Q2M796
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3