Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei405NucleophileBy similarity1
Active sitei458Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • glycogen biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciEcoCyc:GLYCOGEN-BRANCH-MONOMER.
ECOL316407:JW3395-MONOMER.
MetaCyc:GLYCOGEN-BRANCH-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:b3432, JW3395
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10378. glgB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001887021 – 7281,4-alpha-glucan branching enzyme GlgBAdd BLAST728

Proteomic databases

EPDiP07762.
PaxDbiP07762.
PRIDEiP07762.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262503. 311 interactors.
IntActiP07762. 13 interactors.
STRINGi511145.b3432.

Structurei

Secondary structure

1728
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi121 – 123Combined sources3
Beta strandi125 – 134Combined sources10
Beta strandi136 – 143Combined sources8
Beta strandi145 – 148Combined sources4
Beta strandi150 – 154Combined sources5
Helixi155 – 157Combined sources3
Turni161 – 163Combined sources3
Turni170 – 172Combined sources3
Beta strandi174 – 180Combined sources7
Beta strandi187 – 193Combined sources7
Beta strandi199 – 202Combined sources4
Beta strandi208 – 211Combined sources4
Turni213 – 215Combined sources3
Beta strandi217 – 219Combined sources3
Helixi231 – 237Combined sources7
Beta strandi244 – 248Combined sources5
Turni250 – 252Combined sources3
Turni257 – 259Combined sources3
Helixi265 – 278Combined sources14
Beta strandi282 – 287Combined sources6
Helixi295 – 297Combined sources3
Beta strandi303 – 308Combined sources6
Helixi310 – 312Combined sources3
Helixi315 – 327Combined sources13
Beta strandi331 – 336Combined sources6
Beta strandi338 – 340Combined sources3
Helixi351 – 353Combined sources3
Beta strandi356 – 358Combined sources3
Helixi380 – 396Combined sources17
Beta strandi401 – 404Combined sources4
Helixi408 – 411Combined sources4
Helixi433 – 448Combined sources16
Beta strandi454 – 457Combined sources4
Turni464 – 467Combined sources4
Turni470 – 473Combined sources4
Beta strandi478 – 481Combined sources4
Helixi483 – 494Combined sources12
Helixi497 – 502Combined sources6
Helixi505 – 508Combined sources4
Helixi509 – 511Combined sources3
Turni512 – 516Combined sources5
Beta strandi519 – 523Combined sources5
Helixi525 – 527Combined sources3
Helixi535 – 538Combined sources4
Helixi543 – 559Combined sources17
Beta strandi560 – 567Combined sources8
Turni568 – 573Combined sources6
Helixi586 – 589Combined sources4
Beta strandi591 – 593Combined sources3
Helixi595 – 610Combined sources16
Helixi614 – 616Combined sources3
Turni617 – 621Combined sources5
Helixi623 – 625Combined sources3
Beta strandi626 – 633Combined sources8
Turni634 – 637Combined sources4
Beta strandi638 – 644Combined sources7
Beta strandi650 – 656Combined sources7
Beta strandi658 – 660Combined sources3
Beta strandi662 – 665Combined sources4
Beta strandi673 – 680Combined sources8
Helixi685 – 687Combined sources3
Beta strandi713 – 715Combined sources3
Beta strandi719 – 726Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GTYmodel-A24-103[»]
1M7XX-ray2.30A/B/C/D113-728[»]
4LPCX-ray2.39A/B/C/D117-728[»]
4LQ1X-ray2.55A/B/C/D117-728[»]
5E6YX-ray2.60A/B/C/D117-728[»]
5E6ZX-ray1.88A/B/C/D117-728[»]
5E70X-ray2.33A/B/C/D117-728[»]
ProteinModelPortaliP07762.
SMRiP07762.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07762.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C9C. Bacteria.
COG0296. LUCA.
HOGENOMiHOG000283037.
InParanoidiP07762.
KOiK00700.
OMAiHFPSDDF.
PhylomeDBiP07762.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

P07762-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDRIDRDVI NALIAGHFAD PFSVLGMHKT TAGLEVRALL PDATDVWVIE
60 70 80 90 100
PKTGRKLAKL ECLDSRGFFS GVIPRRKNFF RYQLAVVWHG QQNLIDDPYR
110 120 130 140 150
FGPLIQEMDA WLLSEGTHLR PYETLGAHAD TMDGVTGTRF SVWAPNARRV
160 170 180 190 200
SVVGQFNYWD GRRHPMRLRK ESGIWELFIP GAHNGQLYKY EMIDANGNLR
210 220 230 240 250
LKSDPYAFEA QMRPETASLI CGLPEKVVQT EERKKANQFD APISIYEVHL
260 270 280 290 300
GSWRRHTDNN FWLSYRELAD QLVPYAKWMG FTHLELLPIN EHPFDGSWGY
310 320 330 340 350
QPTGLYAPTR RFGTRDDFRY FIDAAHAAGL NVILDWVPGH FPTDDFALAE
360 370 380 390 400
FDGTNLYEHS DPREGYHQDW NTLIYNYGRR EVSNFLVGNA LYWIERFGID
410 420 430 440 450
ALRVDAVASM IYRDYSRKEG EWIPNEFGGR ENLEAIEFLR NTNRILGEQV
460 470 480 490 500
SGAVTMAEES TDFPGVSRPQ DMGGLGFWYK WNLGWMHDTL DYMKLDPVYR
510 520 530 540 550
QYHHDKLTFG ILYNYTENFV LPLSHDEVVH GKKSILDRMP GDAWQKFANL
560 570 580 590 600
RAYYGWMWAF PGKKLLFMGN EFAQGREWNH DASLDWHLLE GGDNWHHGVQ
610 620 630 640 650
RLVRDLNLTY RHHKAMHELD FDPYGFEWLV VDDKERSVLI FVRRDKEGNE
660 670 680 690 700
IIVASNFTPV PRHDYRFGIN QPGKWREILN TDSMHYHGSN AGNGGTVHSD
710 720
EIASHGRQHS LSLTLPPLAT IWLVREAE
Length:728
Mass (Da):84,337
Last modified:August 1, 1988 - v1
Checksum:i0F20AF3677BF2015
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13751 Genomic DNA. Translation: AAA23872.1.
U18997 Genomic DNA. Translation: AAA58230.1.
U00096 Genomic DNA. Translation: AAC76457.1.
AP009048 Genomic DNA. Translation: BAE77860.1.
PIRiA25498. NQECA.
RefSeqiNP_417890.1. NC_000913.3.
WP_001283723.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76457; AAC76457; b3432.
BAE77860; BAE77860; BAE77860.
GeneIDi947940.
KEGGiecj:JW3395.
eco:b3432.
PATRICi32122304. VBIEscCol129921_3529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13751 Genomic DNA. Translation: AAA23872.1.
U18997 Genomic DNA. Translation: AAA58230.1.
U00096 Genomic DNA. Translation: AAC76457.1.
AP009048 Genomic DNA. Translation: BAE77860.1.
PIRiA25498. NQECA.
RefSeqiNP_417890.1. NC_000913.3.
WP_001283723.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GTYmodel-A24-103[»]
1M7XX-ray2.30A/B/C/D113-728[»]
4LPCX-ray2.39A/B/C/D117-728[»]
4LQ1X-ray2.55A/B/C/D117-728[»]
5E6YX-ray2.60A/B/C/D117-728[»]
5E6ZX-ray1.88A/B/C/D117-728[»]
5E70X-ray2.33A/B/C/D117-728[»]
ProteinModelPortaliP07762.
SMRiP07762.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262503. 311 interactors.
IntActiP07762. 13 interactors.
STRINGi511145.b3432.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

EPDiP07762.
PaxDbiP07762.
PRIDEiP07762.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76457; AAC76457; b3432.
BAE77860; BAE77860; BAE77860.
GeneIDi947940.
KEGGiecj:JW3395.
eco:b3432.
PATRICi32122304. VBIEscCol129921_3529.

Organism-specific databases

EchoBASEiEB0373.
EcoGeneiEG10378. glgB.

Phylogenomic databases

eggNOGiENOG4105C9C. Bacteria.
COG0296. LUCA.
HOGENOMiHOG000283037.
InParanoidiP07762.
KOiK00700.
OMAiHFPSDDF.
PhylomeDBiP07762.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciEcoCyc:GLYCOGEN-BRANCH-MONOMER.
ECOL316407:JW3395-MONOMER.
MetaCyc:GLYCOGEN-BRANCH-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP07762.
PROiP07762.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLGB_ECOLI
AccessioniPrimary (citable) accession number: P07762
Secondary accession number(s): Q2M796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 2, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.