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P07762

- GLGB_ECOLI

UniProt

P07762 - GLGB_ECOLI

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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene
glgB, b3432, JW3395
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei405 – 4051Nucleophile By similarity
Active sitei458 – 4581Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: EcoCyc
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: EcoliWiki
  2. glycogen biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciEcoCyc:GLYCOGEN-BRANCH-MONOMER.
ECOL316407:JW3395-MONOMER.
MetaCyc:GLYCOGEN-BRANCH-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:b3432, JW3395
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10378. glgB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7287281,4-alpha-glucan branching enzyme GlgBUniRule annotationPRO_0000188702Add
BLAST

Proteomic databases

PaxDbiP07762.
PRIDEiP07762.

Expressioni

Gene expression databases

GenevestigatoriP07762.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP07762. 13 interactions.
STRINGi511145.b3432.

Structurei

Secondary structure

1
728
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi121 – 1233
Beta strandi125 – 13410
Beta strandi136 – 1438
Beta strandi150 – 1545
Helixi155 – 1573
Turni161 – 1633
Turni170 – 1723
Beta strandi174 – 1807
Beta strandi187 – 1937
Beta strandi199 – 2024
Beta strandi208 – 2114
Turni213 – 2153
Beta strandi217 – 2193
Helixi231 – 2377
Beta strandi244 – 2485
Turni257 – 2593
Helixi265 – 27814
Beta strandi282 – 2876
Helixi295 – 2973
Beta strandi303 – 3086
Helixi310 – 3123
Helixi315 – 32713
Beta strandi331 – 3366
Helixi351 – 3533
Beta strandi356 – 3583
Helixi380 – 39617
Beta strandi401 – 4044
Helixi408 – 4114
Helixi433 – 44816
Beta strandi454 – 4574
Turni464 – 4674
Turni470 – 4734
Beta strandi478 – 4814
Helixi483 – 49412
Helixi497 – 5026
Helixi505 – 5084
Helixi509 – 5113
Turni512 – 5165
Beta strandi519 – 5235
Helixi525 – 5273
Helixi535 – 5384
Helixi543 – 55917
Beta strandi560 – 5678
Turni568 – 5736
Helixi586 – 5894
Helixi595 – 61016
Helixi614 – 6163
Helixi623 – 6253
Beta strandi626 – 6338
Turni634 – 6374
Beta strandi638 – 6447
Beta strandi650 – 6567
Beta strandi662 – 6654
Beta strandi673 – 6808
Helixi685 – 6873
Beta strandi713 – 7153
Beta strandi719 – 7268

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GTYmodel-A24-103[»]
1M7XX-ray2.30A/B/C/D113-728[»]
3O7YX-ray2.41A/B/C/D117-728[»]
3O7ZX-ray2.55A/B/C/D117-728[»]
ProteinModelPortaliP07762.

Miscellaneous databases

EvolutionaryTraceiP07762.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiAKLLFMG.
OrthoDBiEOG6JX7GT.
PhylomeDBiP07762.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

P07762-1 [UniParc]FASTAAdd to Basket

« Hide

MSDRIDRDVI NALIAGHFAD PFSVLGMHKT TAGLEVRALL PDATDVWVIE    50
PKTGRKLAKL ECLDSRGFFS GVIPRRKNFF RYQLAVVWHG QQNLIDDPYR 100
FGPLIQEMDA WLLSEGTHLR PYETLGAHAD TMDGVTGTRF SVWAPNARRV 150
SVVGQFNYWD GRRHPMRLRK ESGIWELFIP GAHNGQLYKY EMIDANGNLR 200
LKSDPYAFEA QMRPETASLI CGLPEKVVQT EERKKANQFD APISIYEVHL 250
GSWRRHTDNN FWLSYRELAD QLVPYAKWMG FTHLELLPIN EHPFDGSWGY 300
QPTGLYAPTR RFGTRDDFRY FIDAAHAAGL NVILDWVPGH FPTDDFALAE 350
FDGTNLYEHS DPREGYHQDW NTLIYNYGRR EVSNFLVGNA LYWIERFGID 400
ALRVDAVASM IYRDYSRKEG EWIPNEFGGR ENLEAIEFLR NTNRILGEQV 450
SGAVTMAEES TDFPGVSRPQ DMGGLGFWYK WNLGWMHDTL DYMKLDPVYR 500
QYHHDKLTFG ILYNYTENFV LPLSHDEVVH GKKSILDRMP GDAWQKFANL 550
RAYYGWMWAF PGKKLLFMGN EFAQGREWNH DASLDWHLLE GGDNWHHGVQ 600
RLVRDLNLTY RHHKAMHELD FDPYGFEWLV VDDKERSVLI FVRRDKEGNE 650
IIVASNFTPV PRHDYRFGIN QPGKWREILN TDSMHYHGSN AGNGGTVHSD 700
EIASHGRQHS LSLTLPPLAT IWLVREAE 728
Length:728
Mass (Da):84,337
Last modified:August 1, 1988 - v1
Checksum:i0F20AF3677BF2015
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13751 Genomic DNA. Translation: AAA23872.1.
U18997 Genomic DNA. Translation: AAA58230.1.
U00096 Genomic DNA. Translation: AAC76457.1.
AP009048 Genomic DNA. Translation: BAE77860.1.
PIRiA25498. NQECA.
RefSeqiNP_417890.1. NC_000913.3.
YP_492001.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76457; AAC76457; b3432.
BAE77860; BAE77860; BAE77860.
GeneIDi12933509.
947940.
KEGGiecj:Y75_p3745.
eco:b3432.
PATRICi32122304. VBIEscCol129921_3529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13751 Genomic DNA. Translation: AAA23872.1 .
U18997 Genomic DNA. Translation: AAA58230.1 .
U00096 Genomic DNA. Translation: AAC76457.1 .
AP009048 Genomic DNA. Translation: BAE77860.1 .
PIRi A25498. NQECA.
RefSeqi NP_417890.1. NC_000913.3.
YP_492001.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GTY model - A 24-103 [» ]
1M7X X-ray 2.30 A/B/C/D 113-728 [» ]
3O7Y X-ray 2.41 A/B/C/D 117-728 [» ]
3O7Z X-ray 2.55 A/B/C/D 117-728 [» ]
ProteinModelPortali P07762.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P07762. 13 interactions.
STRINGi 511145.b3432.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbi P07762.
PRIDEi P07762.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76457 ; AAC76457 ; b3432 .
BAE77860 ; BAE77860 ; BAE77860 .
GeneIDi 12933509.
947940.
KEGGi ecj:Y75_p3745.
eco:b3432.
PATRICi 32122304. VBIEscCol129921_3529.

Organism-specific databases

EchoBASEi EB0373.
EcoGenei EG10378. glgB.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000283037.
KOi K00700.
OMAi AKLLFMG.
OrthoDBi EOG6JX7GT.
PhylomeDBi P07762.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci EcoCyc:GLYCOGEN-BRANCH-MONOMER.
ECOL316407:JW3395-MONOMER.
MetaCyc:GLYCOGEN-BRANCH-MONOMER.

Miscellaneous databases

EvolutionaryTracei P07762.
PROi P07762.

Gene expression databases

Genevestigatori P07762.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-alpha-D-(1,4-alpha-D-glucano)-transferase as deduced from the nucleotide sequence of the glg B gene."
    Baecker P.A., Greenberg E., Preiss J.
    J. Biol. Chem. 261:8738-8743(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The X-ray crystallographic structure of Escherichia coli branching enzyme."
    Abad M.C., Binderup K., Rios-Steiner J., Arni R.K., Preiss J., Geiger J.H.
    J. Biol. Chem. 277:42164-42170(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 113-728.

Entry informationi

Entry nameiGLGB_ECOLI
AccessioniPrimary (citable) accession number: P07762
Secondary accession number(s): Q2M796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: June 11, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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