Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine protease inhibitor A3K

Gene

Serpina3k

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contrapsin inhibits trypsin-like proteases.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei384 – 385Reactive bond2

GO - Molecular functioni

GO - Biological processi

  • response to cytokine Source: MGI
  • response to peptide hormone Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.029.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor A3K
Short name:
Serpin A3K
Alternative name(s):
Contrapsin
SPI-2
Gene namesi
Name:Serpina3k
Synonyms:Mcm2, Spi2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98377. Serpina3k.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000003241722 – 418Serine protease inhibitor A3KAdd BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi39N-linked (GlcNAc...)Sequence analysis1
Glycosylationi105N-linked (GlcNAc...)Sequence analysis1
Glycosylationi185N-linked (GlcNAc...)2 Publications1
Glycosylationi270N-linked (GlcNAc...)2 Publications1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP07759.
PaxDbiP07759.
PeptideAtlasiP07759.
PRIDEiP07759.

PTM databases

iPTMnetiP07759.
PhosphoSitePlusiP07759.

Expressioni

Tissue specificityi

Expressed in liver and secreted in plasma.4 Publications

Gene expression databases

BgeeiENSMUSG00000058207.

Interactioni

Protein-protein interaction databases

BioGridi203440. 2 interactors.
IntActiP07759. 5 interactors.
MINTiMINT-4374070.
STRINGi10090.ENSMUSP00000042095.

Structurei

3D structure databases

ProteinModelPortaliP07759.
SMRiP07759.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni369 – 394RCLAdd BLAST26

Domaini

The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the serpin reactive site and the protease. The resulting inactive serpin-protease complex is highly stable (By similarity). Variability within the reactive center loop (RCL) sequences of Serpina3 paralogs may determine target protease specificity.By similarity

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiP07759.
KOiK04525.
PhylomeDBiP07759.
TreeFamiTF343201.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07759-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFIVAMGMI LMAGICPAVL CFPDGTKEMD IVFHEHQDNG TQDDSLTLAS
60 70 80 90 100
VNTDFAFSLY KKLALKNPDT NIVFSPLSIS AALALVSLGA KGKTMEEILE
110 120 130 140 150
GLKFNLTETP EADIHQGFGN LLQSLSQPED QDQINIGNAM FIEKDLQILA
160 170 180 190 200
EFHEKTRALY QTEAFTADFQ QPTEAKNLIN DYVSNQTQGM IKELISELDE
210 220 230 240 250
RTLMVLVNYI YFKGKWKISF DPQDTFESEF YLDEKRSVKV PMMKMKLLTT
260 270 280 290 300
RHFRDEELSC SVLELKYTGN ASALLILPDQ GRMQQVEASL QPETLRKWRK
310 320 330 340 350
TLFPSQIEEL NLPKFSIASN YRLEEDVLPE MGIKEVFTEQ ADLSGITETK
360 370 380 390 400
KLSVSQVVHK AVLDVAETGT EAAAATGVIG GIRKAILPAV HFNRPFLFVI
410
YHTSAQSILF MAKVNNPK
Length:418
Mass (Da):46,880
Last modified:November 1, 1991 - v2
Checksum:i761A6F986C5B5CCD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15I → V in AAH19802 (PubMed:15489334).Curated1
Sequence conflicti15I → V in AAH11217 (PubMed:15489334).Curated1
Sequence conflicti15I → V in AAH16407 (PubMed:15489334).Curated1
Sequence conflicti68 – 70PDT → QDK in CAA40106 (PubMed:11916263).Curated3
Sequence conflicti68 – 70PDT → QDK in BAE28874 (PubMed:16141072).Curated3
Sequence conflicti72I → F in AAH16407 (PubMed:15489334).Curated1
Sequence conflicti84A → R in CAA38948 (PubMed:2049065).Curated1
Sequence conflicti193E → K in CAA40106 (PubMed:11916263).Curated1
Sequence conflicti193E → K in BAE28874 (PubMed:16141072).Curated1
Sequence conflicti200 – 201ER → DG in CAA40106 (PubMed:11916263).Curated2
Sequence conflicti200 – 201ER → DG in BAE28874 (PubMed:16141072).Curated2
Sequence conflicti204M → V in CAA25458 (PubMed:6547997).Curated1
Sequence conflicti250T → A in CAA40106 (PubMed:11916263).Curated1
Sequence conflicti250T → A in BAE28874 (PubMed:16141072).Curated1
Sequence conflicti304P → S in CAA40106 (PubMed:11916263).Curated1
Sequence conflicti304P → S in BAE28874 (PubMed:16141072).Curated1
Sequence conflicti320N → D in CAA40106 (PubMed:11916263).Curated1
Sequence conflicti320N → D in BAE28874 (PubMed:16141072).Curated1
Sequence conflicti347T → I in CAA25458 (PubMed:6547997).Curated1
Sequence conflicti349T → A in CAA40106 (PubMed:11916263).Curated1
Sequence conflicti349T → A in BAE28874 (PubMed:16141072).Curated1
Sequence conflicti386I → V in CAA40106 (PubMed:11916263).Curated1
Sequence conflicti386I → V in BAE28874 (PubMed:16141072).Curated1
Sequence conflicti389A → G in AAH19802 (PubMed:15489334).Curated1
Sequence conflicti391H → C in CAA40106 (PubMed:11916263).Curated1
Sequence conflicti391H → C in BAE28874 (PubMed:16141072).Curated1
Sequence conflicti398F → I in CAA40106 (PubMed:11916263).Curated1
Sequence conflicti398F → I in BAE28874 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00725 mRNA. Translation: BAA00627.1.
X55147 mRNA. Translation: CAA38948.1.
X56786 mRNA. Translation: CAA40106.1.
AK149436 mRNA. Translation: BAE28874.1.
BC011217 mRNA. Translation: AAH11217.1.
BC016407 mRNA. Translation: AAH16407.1.
BC019802 mRNA. Translation: AAH19802.1.
X00946 Genomic DNA. Translation: CAA25458.1.
CCDSiCCDS36537.1.
PIRiJX0129.
RefSeqiNP_035588.2. NM_011458.2.
UniGeneiMm.291569.

Genome annotation databases

GeneIDi20714.
KEGGimmu:20714.
UCSCiuc007oxd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00725 mRNA. Translation: BAA00627.1.
X55147 mRNA. Translation: CAA38948.1.
X56786 mRNA. Translation: CAA40106.1.
AK149436 mRNA. Translation: BAE28874.1.
BC011217 mRNA. Translation: AAH11217.1.
BC016407 mRNA. Translation: AAH16407.1.
BC019802 mRNA. Translation: AAH19802.1.
X00946 Genomic DNA. Translation: CAA25458.1.
CCDSiCCDS36537.1.
PIRiJX0129.
RefSeqiNP_035588.2. NM_011458.2.
UniGeneiMm.291569.

3D structure databases

ProteinModelPortaliP07759.
SMRiP07759.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203440. 2 interactors.
IntActiP07759. 5 interactors.
MINTiMINT-4374070.
STRINGi10090.ENSMUSP00000042095.

Protein family/group databases

MEROPSiI04.029.

PTM databases

iPTMnetiP07759.
PhosphoSitePlusiP07759.

Proteomic databases

MaxQBiP07759.
PaxDbiP07759.
PeptideAtlasiP07759.
PRIDEiP07759.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20714.
KEGGimmu:20714.
UCSCiuc007oxd.1. mouse.

Organism-specific databases

CTDi20714.
MGIiMGI:98377. Serpina3k.

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiP07759.
KOiK04525.
PhylomeDBiP07759.
TreeFamiTF343201.

Miscellaneous databases

ChiTaRSiMcm2. mouse.
PROiP07759.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000058207.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPA3K_MOUSE
AccessioniPrimary (citable) accession number: P07759
Secondary accession number(s): Q62257
, Q8VCH3, Q91W80, Q91X80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1991
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The single human alpha1-antichymotrypsin gene (SERPINA3) is represented by a cluster of 14 individual murine paralogs.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.