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Protein

Serine protease inhibitor A3K

Gene

Serpina3k

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contrapsin inhibits trypsin-like proteases.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei384 – 3852Reactive bond

GO - Molecular functioni

GO - Biological processi

  • response to cytokine Source: MGI
  • response to peptide hormone Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.029.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor A3K
Short name:
Serpin A3K
Alternative name(s):
Contrapsin
SPI-2
Gene namesi
Name:Serpina3k
Synonyms:Mcm2, Spi2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98377. Serpina3k.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 418397Serine protease inhibitor A3KPRO_0000032417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence analysis
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence analysis
Glycosylationi185 – 1851N-linked (GlcNAc...)2 Publications
Glycosylationi270 – 2701N-linked (GlcNAc...)2 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP07759.
PaxDbiP07759.
PeptideAtlasiP07759.
PRIDEiP07759.

PTM databases

iPTMnetiP07759.
PhosphoSiteiP07759.

Expressioni

Tissue specificityi

Expressed in liver and secreted in plasma.4 Publications

Gene expression databases

BgeeiENSMUSG00000058207.

Interactioni

Protein-protein interaction databases

BioGridi203440. 2 interactions.
IntActiP07759. 5 interactions.
MINTiMINT-4374070.
STRINGi10090.ENSMUSP00000042095.

Structurei

3D structure databases

ProteinModelPortaliP07759.
SMRiP07759. Positions 48-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni369 – 39426RCLAdd
BLAST

Domaini

The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the serpin reactive site and the protease. The resulting inactive serpin-protease complex is highly stable (By similarity). Variability within the reactive center loop (RCL) sequences of Serpina3 paralogs may determine target protease specificity.By similarity

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiP07759.
KOiK04525.
PhylomeDBiP07759.
TreeFamiTF343201.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07759-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFIVAMGMI LMAGICPAVL CFPDGTKEMD IVFHEHQDNG TQDDSLTLAS
60 70 80 90 100
VNTDFAFSLY KKLALKNPDT NIVFSPLSIS AALALVSLGA KGKTMEEILE
110 120 130 140 150
GLKFNLTETP EADIHQGFGN LLQSLSQPED QDQINIGNAM FIEKDLQILA
160 170 180 190 200
EFHEKTRALY QTEAFTADFQ QPTEAKNLIN DYVSNQTQGM IKELISELDE
210 220 230 240 250
RTLMVLVNYI YFKGKWKISF DPQDTFESEF YLDEKRSVKV PMMKMKLLTT
260 270 280 290 300
RHFRDEELSC SVLELKYTGN ASALLILPDQ GRMQQVEASL QPETLRKWRK
310 320 330 340 350
TLFPSQIEEL NLPKFSIASN YRLEEDVLPE MGIKEVFTEQ ADLSGITETK
360 370 380 390 400
KLSVSQVVHK AVLDVAETGT EAAAATGVIG GIRKAILPAV HFNRPFLFVI
410
YHTSAQSILF MAKVNNPK
Length:418
Mass (Da):46,880
Last modified:November 1, 1991 - v2
Checksum:i761A6F986C5B5CCD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151I → V in AAH19802 (PubMed:15489334).Curated
Sequence conflicti15 – 151I → V in AAH11217 (PubMed:15489334).Curated
Sequence conflicti15 – 151I → V in AAH16407 (PubMed:15489334).Curated
Sequence conflicti68 – 703PDT → QDK in CAA40106 (PubMed:11916263).Curated
Sequence conflicti68 – 703PDT → QDK in BAE28874 (PubMed:16141072).Curated
Sequence conflicti72 – 721I → F in AAH16407 (PubMed:15489334).Curated
Sequence conflicti84 – 841A → R in CAA38948 (PubMed:2049065).Curated
Sequence conflicti193 – 1931E → K in CAA40106 (PubMed:11916263).Curated
Sequence conflicti193 – 1931E → K in BAE28874 (PubMed:16141072).Curated
Sequence conflicti200 – 2012ER → DG in CAA40106 (PubMed:11916263).Curated
Sequence conflicti200 – 2012ER → DG in BAE28874 (PubMed:16141072).Curated
Sequence conflicti204 – 2041M → V in CAA25458 (PubMed:6547997).Curated
Sequence conflicti250 – 2501T → A in CAA40106 (PubMed:11916263).Curated
Sequence conflicti250 – 2501T → A in BAE28874 (PubMed:16141072).Curated
Sequence conflicti304 – 3041P → S in CAA40106 (PubMed:11916263).Curated
Sequence conflicti304 – 3041P → S in BAE28874 (PubMed:16141072).Curated
Sequence conflicti320 – 3201N → D in CAA40106 (PubMed:11916263).Curated
Sequence conflicti320 – 3201N → D in BAE28874 (PubMed:16141072).Curated
Sequence conflicti347 – 3471T → I in CAA25458 (PubMed:6547997).Curated
Sequence conflicti349 – 3491T → A in CAA40106 (PubMed:11916263).Curated
Sequence conflicti349 – 3491T → A in BAE28874 (PubMed:16141072).Curated
Sequence conflicti386 – 3861I → V in CAA40106 (PubMed:11916263).Curated
Sequence conflicti386 – 3861I → V in BAE28874 (PubMed:16141072).Curated
Sequence conflicti389 – 3891A → G in AAH19802 (PubMed:15489334).Curated
Sequence conflicti391 – 3911H → C in CAA40106 (PubMed:11916263).Curated
Sequence conflicti391 – 3911H → C in BAE28874 (PubMed:16141072).Curated
Sequence conflicti398 – 3981F → I in CAA40106 (PubMed:11916263).Curated
Sequence conflicti398 – 3981F → I in BAE28874 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00725 mRNA. Translation: BAA00627.1.
X55147 mRNA. Translation: CAA38948.1.
X56786 mRNA. Translation: CAA40106.1.
AK149436 mRNA. Translation: BAE28874.1.
BC011217 mRNA. Translation: AAH11217.1.
BC016407 mRNA. Translation: AAH16407.1.
BC019802 mRNA. Translation: AAH19802.1.
X00946 Genomic DNA. Translation: CAA25458.1.
CCDSiCCDS36537.1.
PIRiJX0129.
RefSeqiNP_035588.2. NM_011458.2.
UniGeneiMm.291569.

Genome annotation databases

GeneIDi20714.
KEGGimmu:20714.
UCSCiuc007oxd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00725 mRNA. Translation: BAA00627.1.
X55147 mRNA. Translation: CAA38948.1.
X56786 mRNA. Translation: CAA40106.1.
AK149436 mRNA. Translation: BAE28874.1.
BC011217 mRNA. Translation: AAH11217.1.
BC016407 mRNA. Translation: AAH16407.1.
BC019802 mRNA. Translation: AAH19802.1.
X00946 Genomic DNA. Translation: CAA25458.1.
CCDSiCCDS36537.1.
PIRiJX0129.
RefSeqiNP_035588.2. NM_011458.2.
UniGeneiMm.291569.

3D structure databases

ProteinModelPortaliP07759.
SMRiP07759. Positions 48-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203440. 2 interactions.
IntActiP07759. 5 interactions.
MINTiMINT-4374070.
STRINGi10090.ENSMUSP00000042095.

Protein family/group databases

MEROPSiI04.029.

PTM databases

iPTMnetiP07759.
PhosphoSiteiP07759.

Proteomic databases

MaxQBiP07759.
PaxDbiP07759.
PeptideAtlasiP07759.
PRIDEiP07759.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20714.
KEGGimmu:20714.
UCSCiuc007oxd.1. mouse.

Organism-specific databases

CTDi20714.
MGIiMGI:98377. Serpina3k.

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiP07759.
KOiK04525.
PhylomeDBiP07759.
TreeFamiTF343201.

Miscellaneous databases

ChiTaRSiMcm2. mouse.
PROiP07759.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000058207.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPA3K_MOUSE
AccessioniPrimary (citable) accession number: P07759
Secondary accession number(s): Q62257
, Q8VCH3, Q91W80, Q91X80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1991
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The single human alpha1-antichymotrypsin gene (SERPINA3) is represented by a cluster of 14 individual murine paralogs.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.