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Protein

Fructose-bisphosphate aldolase, glycosomal

Gene

ALD

Organism
Trypanosoma brucei brucei
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Gpi), Glucose-6-phosphate isomerase, glycosomal (PGI)
  3. ATP-dependent 6-phosphofructokinase (pfk)
  4. Fructose-bisphosphate aldolase, glycosomal (ALD)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66Substrate1
Binding sitei157Substrate1
Active sitei198Proton acceptorBy similarity1
Active sitei240Schiff-base intermediate with dihydroxyacetone-P1
Sitei372Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate1

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: GeneDB

GO - Biological processi

  • glycolytic process Source: GeneDB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

SABIO-RKP07752.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase, glycosomal (EC:4.1.2.13)
Gene namesi
Name:ALD
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

GO - Cellular componenti

  • glycosome Source: GeneDB
Complete GO annotation...

Keywords - Cellular componenti

Glycosome, Peroxisome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4916.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002169352 – 372Fructose-bisphosphate aldolase, glycosomalAdd BLAST371

Interactioni

Chemistry databases

BindingDBiP07752.

Structurei

Secondary structure

1372
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Helixi13 – 15Combined sources3
Helixi23 – 33Combined sources11
Beta strandi39 – 43Combined sources5
Helixi47 – 55Combined sources9
Turni56 – 58Combined sources3
Helixi63 – 74Combined sources12
Helixi79 – 81Combined sources3
Beta strandi83 – 88Combined sources6
Helixi90 – 94Combined sources5
Beta strandi100 – 102Combined sources3
Helixi103 – 109Combined sources7
Beta strandi113 – 117Combined sources5
Beta strandi122 – 124Combined sources3
Beta strandi126 – 129Combined sources4
Beta strandi133 – 135Combined sources3
Helixi141 – 150Combined sources10
Beta strandi155 – 162Combined sources8
Helixi171 – 190Combined sources20
Beta strandi194 – 201Combined sources8
Helixi209 – 230Combined sources22
Helixi234 – 236Combined sources3
Helixi256 – 270Combined sources15
Beta strandi277 – 280Combined sources4
Helixi287 – 297Combined sources11
Beta strandi306 – 313Combined sources8
Helixi314 – 324Combined sources11
Helixi328 – 330Combined sources3
Helixi331 – 349Combined sources19
Helixi355 – 357Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F2JX-ray1.90A2-371[»]
ProteinModelPortaliP07752.
SMRiP07752.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07752.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRVEVLLT QLPAYNRLKT PYEAELIETA KKMTAPGKGL LAADESTGSC
60 70 80 90 100
SKRFAGIGLS NTAEHRRQYR ALMLECEGFE QYISGVILHD ETVYQKAKTG
110 120 130 140 150
ETFPQYLRRR GVVPGIKTDC GLEPLVEGAK GEQMTAGLDG YIKRAKKYYA
160 170 180 190 200
MGCRFCKWRN VYKIQNGTVS EAVVRFNAET LARYAILSQL CGLVPIVEPE
210 220 230 240 250
VMIDGTHDIE TCQRVSQHVW SEVVSALHRH GVVWEGCLLK PNMVVPGAES
260 270 280 290 300
GLKGHAEQVA EYTVKTLARV IPPALPGVTF LSGGLSEVMA SEYLNAMNNC
310 320 330 340 350
PLPRPWKLTF SYARALQSSA IKRWGGKESG VEAGRRAFMH RAKMNSLAQL
360 370
GKYNRADDDK DSQSLYVAGN TY
Length:372
Mass (Da):41,178
Last modified:January 23, 2007 - v2
Checksum:i30F1F26F0D54211D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti254 – 255GH → AT in AAA30153 (PubMed:3386689).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03061 mRNA. Translation: CAA26867.1.
X52586 Genomic DNA. Translation: CAA36819.1.
M19994 Genomic DNA. Translation: AAA30153.1.
PIRiA24678. ADUT.
A54500.

Genome annotation databases

GeneDBiTb927.10.5620:pep.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03061 mRNA. Translation: CAA26867.1.
X52586 Genomic DNA. Translation: CAA36819.1.
M19994 Genomic DNA. Translation: AAA30153.1.
PIRiA24678. ADUT.
A54500.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F2JX-ray1.90A2-371[»]
ProteinModelPortaliP07752.
SMRiP07752.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP07752.
ChEMBLiCHEMBL4916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneDBiTb927.10.5620:pep.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
SABIO-RKP07752.

Miscellaneous databases

EvolutionaryTraceiP07752.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALF_TRYBB
AccessioniPrimary (citable) accession number: P07752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.