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P07750 (IL4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interleukin-4

Short name=IL-4
Alternative name(s):
B-cell IgG differentiation factor
B-cell growth factor 1
B-cell stimulatory factor 1
Short name=BSF-1
IGG1 induction factor
Lymphocyte stimulatory factor 1
Gene names
Name:Il4
Synonyms:Il-4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length140 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

Subcellular location

Secreted.

Sequence similarities

Belongs to the IL-4/IL-13 family.

Ontologies

Keywords
   Biological processB-cell activation
   Cellular componentSecreted
   DomainSignal
   Molecular functionCytokine
Growth factor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processB cell activation

Inferred from direct assay PubMed 16129705PubMed 9798653. Source: MGI

B cell costimulation

Inferred from direct assay PubMed 18762567. Source: MGI

T-helper 1 cell lineage commitment

Inferred from genetic interaction PubMed 20706986. Source: MGI

T-helper 2 cell cytokine production

Inferred from electronic annotation. Source: Ensembl

T-helper 2 cell differentiation

Inferred from direct assay PubMed 20706986. Source: MGI

cellular response to mercury ion

Inferred from electronic annotation. Source: Ensembl

cholesterol metabolic process

Inferred from mutant phenotype PubMed 11971948. Source: UniProtKB

defense response to protozoan

Inferred from mutant phenotype PubMed 7927664. Source: MGI

dendritic cell differentiation

Inferred from electronic annotation. Source: Ensembl

extrinsic apoptotic signaling pathway in absence of ligand

Inferred from direct assay PubMed 12115603. Source: MGI

female pregnancy

Inferred from electronic annotation. Source: Ensembl

innate immune response in mucosa

Inferred from direct assay PubMed 9973465. Source: BHF-UCL

negative regulation of T cell activation

Inferred from direct assay PubMed 18997793. Source: MGI

negative regulation of T-helper 17 cell differentiation

Inferred from direct assay PubMed 18792410. Source: MGI

negative regulation of acute inflammatory response

Inferred from electronic annotation. Source: Ensembl

negative regulation of chronic inflammatory response

Inferred from electronic annotation. Source: Ensembl

negative regulation of extrinsic apoptotic signaling pathway

Inferred from direct assay PubMed 10429671. Source: MGI

negative regulation of macrophage activation

Inferred from electronic annotation. Source: Ensembl

negative regulation of nitric oxide biosynthetic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of osteoclast differentiation

Inferred from direct assay PubMed 12941478. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

positive regulation of B cell activation

Inferred from direct assay PubMed 8566034. Source: MGI

positive regulation of B cell proliferation

Inferred from direct assay PubMed 10979977PubMed 14607964. Source: MGI

positive regulation of MHC class II biosynthetic process

Inferred from direct assay PubMed 10549626PubMed 11027433. Source: MGI

positive regulation of T cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of T cell proliferation

Inferred from direct assay PubMed 1588041PubMed 18997793. Source: MGI

positive regulation of activated T cell proliferation

Inferred from direct assay PubMed 10485657PubMed 9841920. Source: MGI

positive regulation of chemokine biosynthetic process

Inferred from direct assay PubMed 9013979. Source: BHF-UCL

positive regulation of interleukin-10 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of interleukin-13 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of isotype switching to IgE isotypes

Inferred from direct assay PubMed 14988498. Source: MGI

positive regulation of isotype switching to IgG isotypes

Inferred from direct assay PubMed 12882831PubMed 14988498. Source: MGI

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 8022486. Source: MGI

positive regulation of protein phosphorylation

Inferred from direct assay PubMed 12115603. Source: MGI

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 7749983. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15187136. Source: BHF-UCL

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 20696860. Source: UniProtKB

positive regulation of tyrosine phosphorylation of Stat5 protein

Inferred from direct assay PubMed 10485657. Source: MGI

regulation of immune response

Inferred from direct assay PubMed 12882831. Source: MGI

regulation of proton transport

Inferred from electronic annotation. Source: Ensembl

response to cytokine

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to nutrient

Inferred from electronic annotation. Source: Ensembl

response to organic cyclic compound

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentexternal side of plasma membrane

Inferred from direct assay PubMed 19666510PubMed 20399120PubMed 20956546PubMed 21217760PubMed 21613615. Source: MGI

extracellular space

Inferred from direct assay PubMed 11057672PubMed 11894098PubMed 16275384PubMed 16769892PubMed 17082577PubMed 17277142PubMed 19139201PubMed 21460847. Source: MGI

   Molecular_functioncytokine activity

Inferred from direct assay PubMed 10485657PubMed 10549626PubMed 10979977PubMed 11027433PubMed 1588041PubMed 9841920. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020
Chain21 – 140120Interleukin-4
PRO_0000015538

Amino acid modifications

Glycosylation611N-linked (GlcNAc...) Potential
Glycosylation911N-linked (GlcNAc...) Potential
Glycosylation1171N-linked (GlcNAc...) Potential
Disulfide bond25 ↔ 107 Ref.6
Disulfide bond47 ↔ 87 Ref.6
Disulfide bond69 ↔ 114 Ref.6

Sequences

Sequence LengthMass (Da)Tools
P07750 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: E43CE16195DE051F

FASTA14015,834
        10         20         30         40         50         60 
MGLNPQLVVI LLFFLECTRS HIHGCDKNHL REIIGILNEV TGEGTPCTEM DVPNVLTATK 

        70         80         90        100        110        120 
NTTESELVCR ASKVLRIFYL KHGKTPCLKK NSSVLMELQR LFRAFRCLDS SISCTMNESK 

       130        140 
STSLKDFLES LKSIMQMDYS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter."
Noma Y., Sideras P., Naito T., Bergstedt-Lindqvist S., Azuma C., Severinson E., Tanabe T., Kinashi T., Matsuda F., Yaoita Y., Honjo T.
Nature 319:640-646(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structural analysis of the mouse chromosomal gene encoding interleukin 4 which expresses B cell, T cell and mast cell stimulating activities."
Otsuka T., Villaret D., Yokota T., Takebe Y., Lee F., Arai N., Arai K.
Nucleic Acids Res. 15:333-344(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
[3]"Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities."
Lee F., Yokota T., Otsuka T., Meyerson P., Villaret D., Coffman R., Mosmann T., Rennick D., Roehm N., Smith C., Zlotnik A., Arai K.
Proc. Natl. Acad. Sci. U.S.A. 83:2061-2065(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"IgG1 induction factor: a single molecular entity with multiple biological functions."
Sideras P., Bergstedt-Lindqvist S., Severinson E., Noma Y., Naito T., Azuma C., Tanabe T., Kinashi T., Matsude F., Yaoita Y., Honjo T.
Adv. Exp. Med. Biol. 213:227-236(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Thymus.
[6]"Disulfide assignments in recombinant mouse and human interleukin 4."
Carr C., Aykent S., Kimack N.M., Levine A.D.
Biochemistry 30:1515-1523(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03532 mRNA. Translation: CAA27233.1.
X05064 Genomic DNA. Translation: CAA28731.1.
X05252 Genomic DNA. Translation: CAA28873.1. Sequence problems.
X05253 Genomic DNA. Translation: CAA28874.1. Sequence problems.
M13238 mRNA. Translation: AAA39307.1.
M25892 mRNA. Translation: AAA39298.1.
BC027514 mRNA. Translation: AAH27514.1.
PIRIIMSG1. A25870.
RefSeqNP_067258.1. NM_021283.2.
UniGeneMm.276360.

3D structure databases

ProteinModelPortalP07750.
SMRP07750. Positions 23-140.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP07750. 1 interaction.

Proteomic databases

PRIDEP07750.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000889; ENSMUSP00000000889; ENSMUSG00000000869.
GeneID16189.
KEGGmmu:16189.
UCSCuc007iwq.2. mouse.

Organism-specific databases

CTD3565.
MGIMGI:96556. Il4.

Phylogenomic databases

eggNOGNOG266404.
GeneTreeENSGT00390000013108.
HOGENOMHOG000254781.
HOVERGENHBG000290.
InParanoidP07750.
KOK05430.
OrthoDBEOG7SJD75.
PhylomeDBP07750.
TreeFamTF336383.

Gene expression databases

BgeeP07750.
CleanExMM_IL4.
GenevestigatorP07750.

Family and domain databases

Gene3D1.20.1250.10. 1 hit.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR002354. IL-4.
IPR001325. IL-4/IL-13.
IPR018096. IL-4/IL-13_CS.
[Graphical view]
PANTHERPTHR11456. PTHR11456. 1 hit.
PfamPF00727. IL4. 1 hit.
[Graphical view]
PIRSFPIRSF001941. Interleukin_4. 1 hit.
PRINTSPR00431. INTERLEUKIN4.
SMARTSM00190. IL4_13. 1 hit.
[Graphical view]
SUPFAMSSF47266. SSF47266. 1 hit.
PROSITEPS00838. INTERLEUKIN_4_13. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio289093.
PROP07750.
SOURCESearch...

Entry information

Entry nameIL4_MOUSE
AccessionPrimary (citable) accession number: P07750
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: April 16, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot