P07750 (IL4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-4
Short name=IL-4

Alternative name(s):
B-cell IgG differentiation factor
B-cell growth factor 1
B-cell stimulatory factor 1
Short name=BSF-1
IGG1 induction factor
Lymphocyte stimulatory factor 1

Gene names

Name:	Il4
Synonyms:	Il-4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Subcellular location	Secreted.
Sequence similarities	Belongs to the IL-4/IL-13 family.

Ontologies

Keywords
Biological process	B-cell activation
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	B cell activation Inferred from direct assay PubMed 9798653. Source: MGI B cell costimulation Inferred from direct assay PubMed 18762567. Source: MGI T-helper 1 cell lineage commitment Inferred from genetic interaction PubMed 20706986. Source: MGI T-helper 2 cell cytokine production Inferred from electronic annotation. Source: Compara T-helper 2 cell differentiation Inferred from direct assay PubMed 20706986. Source: MGI cellular response to mercury ion Inferred from electronic annotation. Source: Compara cholesterol metabolic process Inferred from mutant phenotype PubMed 11971948. Source: UniProtKB defense response to protozoan Inferred from mutant phenotype PubMed 7927664. Source: MGI dendritic cell differentiation Inferred from electronic annotation. Source: Compara female pregnancy Inferred from electronic annotation. Source: Compara innate immune response in mucosa Inferred from direct assay PubMed 9973465. Source: BHF-UCL negative regulation of T-helper 17 cell differentiation Inferred from direct assay PubMed 18792410. Source: MGI negative regulation of acute inflammatory response Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from direct assay PubMed 10429671. Source: MGI negative regulation of chronic inflammatory response Inferred from electronic annotation. Source: Compara negative regulation of macrophage activation Inferred from electronic annotation. Source: Compara negative regulation of nitric oxide biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of osteoclast differentiation Inferred from direct assay PubMed 12941478. Source: MGI negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara positive regulation of B cell proliferation Inferred from direct assay PubMed 10979977PubMed 14607964. Source: MGI positive regulation of MHC class II biosynthetic process Inferred from direct assay PubMed 10549626PubMed 11027433. Source: MGI positive regulation of T cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of activated T cell proliferation Inferred from direct assay PubMed 10485657PubMed 9841920. Source: MGI positive regulation of chemokine biosynthetic process Inferred from direct assay PubMed 9013979. Source: BHF-UCL positive regulation of interleukin-10 production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-13 production Inferred from electronic annotation. Source: Compara positive regulation of isotype switching to IgE isotypes Inferred from direct assay PubMed 14988498. Source: MGI positive regulation of isotype switching to IgG isotypes Inferred from direct assay PubMed 12882831PubMed 14988498. Source: MGI positive regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 7749983. Source: BHF-UCL positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 15187136. Source: BHF-UCL positive regulation of tyrosine phosphorylation of Stat5 protein Inferred from direct assay PubMed 10485657. Source: MGI regulation of proton transport Inferred from electronic annotation. Source: Compara response to cytokine stimulus Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to nutrient Inferred from electronic annotation. Source: Compara response to organic cyclic compound Inferred from electronic annotation. Source: Compara
Cellular_component	external side of plasma membrane Inferred from direct assay PubMed 19666510PubMed 20399120PubMed 20956546PubMed 21217760PubMed 21613615. Source: MGI extracellular space Inferred from direct assay PubMed 11057672PubMed 11894098PubMed 16275384PubMed 16769892PubMed 17082577PubMed 17277142PubMed 19139201PubMed 21460847. Source: MGI
Molecular_function	cytokine activity Inferred from direct assay PubMed 10485657PubMed 10549626PubMed 10979977PubMed 11027433PubMed 1588041PubMed 9841920. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 140	120	Interleukin-4		PRO_0000015538
Amino acid modifications
	Glycosylation	61	1	N-linked (GlcNAc...) Potential
	Glycosylation	91	1	N-linked (GlcNAc...) Potential
	Glycosylation	117	1	N-linked (GlcNAc...) Potential
	Disulfide bond	25 ↔ 107		Ref.6
	Disulfide bond	47 ↔ 87		Ref.6
	Disulfide bond	69 ↔ 114		Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P07750 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: E43CE16195DE051F	FASTA	140	15,834
	10 20 30 40 50 60 MGLNPQLVVI LLFFLECTRS HIHGCDKNHL REIIGILNEV TGEGTPCTEM DVPNVLTATK 70 80 90 100 110 120 NTTESELVCR ASKVLRIFYL KHGKTPCLKK NSSVLMELQR LFRAFRCLDS SISCTMNESK 130 140 STSLKDFLES LKSIMQMDYS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter." Noma Y., Sideras P., Naito T., Bergstedt-Lindqvist S., Azuma C., Severinson E., Tanabe T., Kinashi T., Matsuda F., Yaoita Y., Honjo T. Nature 319:640-646(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of the mouse chromosomal gene encoding interleukin 4 which expresses B cell, T cell and mast cell stimulating activities." Otsuka T., Villaret D., Yokota T., Takebe Y., Lee F., Arai N., Arai K. Nucleic Acids Res. 15:333-344(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c.
[3]	"Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities." Lee F., Yokota T., Otsuka T., Meyerson P., Villaret D., Coffman R., Mosmann T., Rennick D., Roehm N., Smith C., Zlotnik A., Arai K. Proc. Natl. Acad. Sci. U.S.A. 83:2061-2065(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"IgG1 induction factor: a single molecular entity with multiple biological functions." Sideras P., Bergstedt-Lindqvist S., Severinson E., Noma Y., Naito T., Azuma C., Tanabe T., Kinashi T., Matsude F., Yaoita Y., Honjo T. Adv. Exp. Med. Biol. 213:227-236(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus.
[6]	"Disulfide assignments in recombinant mouse and human interleukin 4." Carr C., Aykent S., Kimack N.M., Levine A.D. Biochemistry 30:1515-1523(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03532 mRNA. Translation: CAA27233.1. X05064 Genomic DNA. Translation: CAA28731.1. X05252 Genomic DNA. Translation: CAA28873.1. Sequence problems. X05253 Genomic DNA. Translation: CAA28874.1. Sequence problems. M13238 mRNA. Translation: AAA39307.1. M25892 mRNA. Translation: AAA39298.1. BC027514 mRNA. Translation: AAH27514.1.
IPI	IPI00131815.
PIR	IIMSG1. A25870.
RefSeq	NP_067258.1. NM_021283.2.
UniGene	Mm.276360.
3D structure databases
ProteinModelPortal	P07750.
SMR	P07750. Positions 23-140.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07750. 1 interaction.
Proteomic databases
PRIDE	P07750.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000000889; ENSMUSP00000000889; ENSMUSG00000000869.
GeneID	16189.
KEGG	mmu:16189.
Organism-specific databases
CTD	3565.
MGI	MGI:96556. Il4.
Phylogenomic databases
eggNOG	NOG266404.
GeneTree	ENSGT00390000013108.
HOGENOM	HOG000254781.
HOVERGEN	HBG000290.
InParanoid	P07750.
KO	K05430.
OrthoDB	EOG469QWD.
Gene expression databases
ArrayExpress	P07750.
Bgee	P07750.
CleanEx	MM_IL4.
Genevestigator	P07750.
GermOnline	ENSMUSG00000000869. Mus musculus.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR002354. Interleukin-4. IPR018096. Interleukin-4/-13_CS. IPR001325. Interleukin-4/13. [Graphical view]
PANTHER	PTHR11456. PTHR11456. 1 hit.
Pfam	PF00727. IL4. 1 hit. [Graphical view]
PIRSF	PIRSF001941. Interleukin_4. 1 hit.
PRINTS	PR00431. INTERLEUKIN4.
SMART	SM00190. IL4_13. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00838. INTERLEUKIN_4_13. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	289093.
SOURCE	Search...

Entry information

Entry name

IL4_MOUSE

Accession

Primary (citable) accession number: P07750

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	May 1, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program