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Protein

Keratin, type II cytoskeletal 4

Gene

Krt4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei397 – 3971Stutter

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • structural molecule activity Source: InterPro

GO - Biological processi

  • cytoskeleton organization Source: MGI
  • embryo development Source: UniProtKB
  • epithelial cell differentiation Source: UniProtKB
  • negative regulation of epithelial cell proliferation Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type II cytoskeletal 4
Alternative name(s):
Cytokeratin-4
Short name:
CK-4
Cytoskeletal 57 kDa keratin
Keratin-4
Short name:
K4
Type-II keratin Kb4
Gene namesi
Name:Krt4
Synonyms:Krt2-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:96701. Krt4.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: MGI
  • intermediate filament cytoskeleton Source: MGI
  • keratin filament Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament, Keratin

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Keratin, type II cytoskeletal 4PRO_0000063723Add
BLAST

Proteomic databases

MaxQBiP07744.
PaxDbiP07744.
PRIDEiP07744.

PTM databases

iPTMnetiP07744.
PhosphoSiteiP07744.

Expressioni

Tissue specificityi

Expressed in the dorsal and ventral epithelium of the tongue. Highest expression levels are detected in the suprabasal layer with low levels detected in the basal cell layer. Within the suprabasal layer expression is highest in the spinous cells, decreases in the granular cells and is not detected in the stratum corneum.1 Publication

Gene expression databases

BgeeiP07744.
CleanExiMM_KRT4.
GenevisibleiP07744. MM.

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. Keratin-4 is generally associated with keratin-13.

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi201034. 1 interaction.
IntActiP07744. 2 interactions.
STRINGi10090.ENSMUSP00000023797.

Structurei

3D structure databases

ProteinModelPortaliP07744.
SMRiP07744. Positions 143-285, 312-454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 145145HeadAdd
BLAST
Regioni146 – 455310RodAdd
BLAST
Regioni146 – 18136Coil 1AAdd
BLAST
Regioni182 – 20019Linker 1Add
BLAST
Regioni201 – 29292Coil 1BAdd
BLAST
Regioni293 – 31624Linker 12Add
BLAST
Regioni317 – 455139Coil 2Add
BLAST
Regioni456 – 52469TailAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi10 – 117108Gly-richAdd
BLAST
Compositional biasi473 – 51038Gly-richAdd
BLAST

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGG3. Eukaryota.
ENOG410ZE7B. LUCA.
GeneTreeiENSGT00760000118796.
HOGENOMiHOG000230976.
HOVERGENiHBG013015.
InParanoidiP07744.
KOiK07605.
OMAiQQCVRGG.
OrthoDBiEOG7FV3Q8.
PhylomeDBiP07744.
TreeFamiTF317854.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR032444. Keratin_2_head.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF16208. Keratin_2_head. 1 hit.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIARQSSVRG ASRGFSSGSA IAGGVKRVAF SSGSMSGGAG RCSSGGFGSR
60 70 80 90 100
SLYNLGGHKS ISMSVAGSCQ GGGYGGAGGF GVGGYGAGFG AGGFGGGFGG
110 120 130 140 150
SFNGRGGPGF PVCPAGGIQE VTINQSLLTP LQVEIDPEIQ KIRTAEREQI
160 170 180 190 200
KTLNNKFASF IDKVRFLEQQ NKVLETKWNL LQQQTTTTSP KSLDPFFETY
210 220 230 240 250
INALRKNLDT LSNDKGRLQS ELKMMQDSVE DFKTKYEEEI NKRTAAENDF
260 270 280 290 300
VVLKKDVDAA YMIKVELEAK MESLKDEINF TRVLYEAELA QMQTHVSDTS
310 320 330 340 350
VVLSMDNNRN LDLDGIIAEV RAQYEDIARK SKAEVESWYQ IKVQQLQMSA
360 370 380 390 400
DQHGDSLKTT KNEISELNRM IQRLRAEIEN IKKQSQTLQA SVADAEQRGE
410 420 430 440 450
LALKDAYSKR AELETALQKA KEDLARLLRD YQALMNVKLA LDVEIATYRK
460 470 480 490 500
LLEGEECRMS GECKSAVSIS VVGGSASIGG SGLGLGSGFC SGSGSGSGFG
510 520
FGGGIYGGSG SKITSSATIT KRSPR
Length:525
Mass (Da):56,283
Last modified:November 22, 2005 - v2
Checksum:i27A3A3DF7E87DF90
GO

Sequence cautioni

The sequence AAH64008.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131R → P in CAA27207 (PubMed:2418416).Curated
Sequence conflicti16 – 161S → T in CAA27207 (PubMed:2418416).Curated
Sequence conflicti79 – 791G → D in AAH64008 (PubMed:15489334).Curated
Sequence conflicti388 – 3881L → P in CAA27207 (PubMed:2418416).Curated
Sequence conflicti476 – 4805ASIGG → QHWR in CAA27207 (PubMed:2418416).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03491 mRNA. Translation: CAA27207.1.
BC064008 mRNA. Translation: AAH64008.1. Different initiation.
BC145839 mRNA. Translation: AAI45840.1.
BC145841 mRNA. Translation: AAI45842.1.
CCDSiCCDS37222.1.
PIRiA23518.
RefSeqiNP_032501.2. NM_008475.2.
UniGeneiMm.46425.

Genome annotation databases

EnsembliENSMUST00000023797; ENSMUSP00000023797; ENSMUSG00000059668.
GeneIDi16682.
KEGGimmu:16682.
UCSCiuc007xuf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03491 mRNA. Translation: CAA27207.1.
BC064008 mRNA. Translation: AAH64008.1. Different initiation.
BC145839 mRNA. Translation: AAI45840.1.
BC145841 mRNA. Translation: AAI45842.1.
CCDSiCCDS37222.1.
PIRiA23518.
RefSeqiNP_032501.2. NM_008475.2.
UniGeneiMm.46425.

3D structure databases

ProteinModelPortaliP07744.
SMRiP07744. Positions 143-285, 312-454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201034. 1 interaction.
IntActiP07744. 2 interactions.
STRINGi10090.ENSMUSP00000023797.

PTM databases

iPTMnetiP07744.
PhosphoSiteiP07744.

Proteomic databases

MaxQBiP07744.
PaxDbiP07744.
PRIDEiP07744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023797; ENSMUSP00000023797; ENSMUSG00000059668.
GeneIDi16682.
KEGGimmu:16682.
UCSCiuc007xuf.1. mouse.

Organism-specific databases

CTDi3851.
MGIiMGI:96701. Krt4.

Phylogenomic databases

eggNOGiENOG410IGG3. Eukaryota.
ENOG410ZE7B. LUCA.
GeneTreeiENSGT00760000118796.
HOGENOMiHOG000230976.
HOVERGENiHBG013015.
InParanoidiP07744.
KOiK07605.
OMAiQQCVRGG.
OrthoDBiEOG7FV3Q8.
PhylomeDBiP07744.
TreeFamiTF317854.

Miscellaneous databases

ChiTaRSiKrt4. mouse.
NextBioi290433.
PROiP07744.
SOURCEiSearch...

Gene expression databases

BgeeiP07744.
CleanExiMM_KRT4.
GenevisibleiP07744. MM.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR032444. Keratin_2_head.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF16208. Keratin_2_head. 1 hit.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nonepidermal members of the keratin multigene family: cDNA sequences and in situ localization of the mRNAs."
    Knapp B., Rentrop M., Schweizer J., Winter H.
    Nucleic Acids Res. 14:751-763(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  3. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 157-163; 410-421 AND 439-449, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiK2C4_MOUSE
AccessioniPrimary (citable) accession number: P07744
Secondary accession number(s): A6H6D6, Q6P3F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 22, 2005
Last modified: May 11, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.