Reviewed, UniProtKB/Swiss-Prot P07740 (LUXA_VIBHA)

Names and origin

Protein names

Recommended name:
    Alkanal monooxygenase alpha chain
    EC=1.14.14.3
Alternative name(s):
    Bacterial luciferase alpha chain

Gene names

Name:

luxA

Organism

Vibrio harveyi

Taxonomic identifier

669 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria.
Catalytic activity	RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.
Subunit structure	Heterodimer of an alpha and a beta chain.
Miscellaneous	The synthesis of this protein is regulated by a complex control mechanism that has been termed autoinduction. In fully induced cells luciferase comprises up to 5% of the soluble protein.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 355

355

Alkanal monooxygenase alpha chain

PRO_0000220172

Experimental info

Sequence conflict

17

1

Q → E AA sequence Ref.1

Sequence conflict

68

1

T → K in CAA41597. Ref.2

Sequence conflict

169

1

P → A AA sequence Ref.1

Sequence conflict

174

1

A → P AA sequence Ref.1

Sequence conflict

204 – 205

2

QL → HV in CAA41597. Ref.2

Sequence conflict

238

1

R → K in CAA41597. Ref.2

Sequence conflict

321

1

D → N in CAA41597. Ref.2

Secondary structure

1

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355

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Helix

17 – 33

17

Beta strand

39 – 42

4

Helix

55 – 64

10

Beta strand

71 – 78

8

Helix

83 – 97

15

Beta strand

101 – 106

6

Helix

111 – 117

7

Helix

124 – 141

18

Beta strand

142 – 144

3

Beta strand

148 – 150

3

Beta strand

170 – 173

4

Helix

177 – 185

9

Beta strand

190 – 192

3

Beta strand

194 – 196

3

Helix

198 – 214

17

Turn

219 – 221

3

Beta strand

225 – 229

5

Helix

236 – 256

21

Beta strand

262 – 265

4

Helix

295 – 298

4

Beta strand

299 – 303

5

Helix

304 – 318

15

Beta strand

322 – 326

5

Helix

328 – 330

3

Helix

333 – 346

14

Sequences

	Sequence		Length	Mass (Da)
	P07740-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: F915E6BBE41ACEEE	FASTA	355	40,153
	10 20 30 40 50 60 MKFGNFLLTY QPPELSQTEV MKRLVNLGKA SEGCGFDTVW LLEHHFTEFG LLGNPYVAAA 70 80 90 100 110 120 HLLGATETLN VGTAAIVLPT AHPVRQAEDV NLLDQMSKGR FRFGICRGLY DKDFRVFGTD 130 140 150 160 170 180 MDNSRALMDC WYDLMKEGFN EGYIAADNEH IKFPKIQLNP SAYTQGGAPV YVVAESASTT 190 200 210 220 230 240 EWAAERGLPM ILSWIINTHE KKAQLDLYNE VATEHGYDVT KIDHCLSYIT SVDHDSNRAK 250 260 270 280 290 300 DICRNFLGHW YDSYVNATKI FDDSDQTKGY DFNKGQWRDF VLKGHKDTNR RIDYSYEINP 310 320 330 340 350 VGTPEECIAI IQQDIDATGI DNICCGFEAN GSEEEIIASM KLFQSDVMPY LKEKQ

References

[1]	"Nucleotide sequence of the luxA gene of Vibrio harveyi and the complete amino acid sequence of the alpha subunit of bacterial luciferase." Cohn D.H., Mileham A.J., Simon M.I., Nealson K.H., Rausch S.K., Bonam D., Baldwin T.O. J. Biol. Chem. 260:6139-6146(1985) [PubMed: 3997817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The beta subunit polypeptide of Vibrio harveyi luciferase determines light emission at 42 degrees C." Escher A., O'Kane D.J., Szalay A.A. Mol. Gen. Genet. 230:385-393(1991) [PubMed: 1685011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CTP5.
[3]	"Cloning of the Vibrio harveyi luciferase genes: use of a synthetic oligonucleotide probe." Cohn D.H., Ogden R.C., Abelson J.N., Baldwin T.O., Nealson K.H., Simon M.I., Mileham A.J. Proc. Natl. Acad. Sci. U.S.A. 80:120-123(1983) [PubMed: 6571986] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28 AND 74-133. Strain: ATCC 33843 / 392 / MAV.
[4]	"Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4-A resolution." Fisher A.J., Raushel F.M., Baldwin T.O., Rayment I. Biochemistry 34:6581-6586(1995) [PubMed: 7756289] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
[5]	"The 1.5-A resolution crystal structure of bacterial luciferase in low salt conditions." Fisher A.J., Thompson T.B., Thoden J.B., Baldwin T.O., Rayment I. J. Biol. Chem. 271:21956-21968(1996) [PubMed: 8703001] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

M10961 Genomic DNA. Translation: AAA88685.1.
X58791 Genomic DNA. Translation: CAA41597.1.
V01422 Genomic DNA. Translation: CAA24692.1.
V01423 Genomic DNA. Translation: CAA24693.1.

PIR

A22613.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BRL	X-ray	2.40	A/C	1-355	[»]
1LUC	X-ray	1.50	A	1-355	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.14.14.3. 351.

Family and domain databases

InterPro

IPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like.
IPR016048. Luciferase-like_sub.
IPR002103. Luciferase_bac.
[Graphical view]

Gene3D

G3DSA:3.20.20.30. Luciferase_like. 1 hit.

Pfam

PF00296. Bac_luciferase. 1 hit.
[Graphical view]

PRINTS

PR00089. LUCIFERASE.

PROSITE

PS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LUXA_VIBHA

Accession

Primary (citable) accession number: P07740
Secondary accession number(s): Q56698, Q6LBZ4, Q6LBZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)