Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alkanal monooxygenase alpha chain

Gene

luxA

Organism
Vibrio harveyi (Beneckea harveyi)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Light-emitting reaction in luminous bacteria.By similarity

Catalytic activityi

A long-chain aldehyde + FMNH2 + O2 = a long-chain fatty acid + FMN + H2O + light.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase, Photoprotein

Keywords - Biological processi

Luminescence

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BRENDAi1.14.14.3. 6632.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkanal monooxygenase alpha chain (EC:1.14.14.3By similarity)
Alternative name(s):
Bacterial luciferase alpha chain
Gene namesi
Name:luxA
OrganismiVibrio harveyi (Beneckea harveyi)
Taxonomic identifieri669 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002201721 – 355Alkanal monooxygenase alpha chainAdd BLAST355

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.2 Publications

Protein-protein interaction databases

MINTiMINT-1518824.
STRINGi338187.VIBHAR_06242.

Chemistry databases

BindingDBiP07740.

Structurei

Secondary structure

1355
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi17 – 30Combined sources14
Helixi32 – 34Combined sources3
Beta strandi37 – 41Combined sources5
Helixi55 – 65Combined sources11
Beta strandi70 – 77Combined sources8
Helixi78 – 80Combined sources3
Helixi83 – 97Combined sources15
Beta strandi101 – 106Combined sources6
Helixi111 – 117Combined sources7
Helixi121 – 123Combined sources3
Helixi124 – 141Combined sources18
Beta strandi142 – 150Combined sources9
Beta strandi152 – 157Combined sources6
Beta strandi170 – 172Combined sources3
Helixi177 – 185Combined sources9
Beta strandi190 – 192Combined sources3
Beta strandi194 – 196Combined sources3
Helixi198 – 214Combined sources17
Helixi219 – 221Combined sources3
Beta strandi225 – 229Combined sources5
Helixi236 – 259Combined sources24
Turni260 – 263Combined sources4
Helixi274 – 276Combined sources3
Helixi294 – 298Combined sources5
Beta strandi299 – 303Combined sources5
Helixi304 – 318Combined sources15
Beta strandi322 – 326Combined sources5
Helixi328 – 330Combined sources3
Helixi333 – 346Combined sources14
Helixi348 – 350Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BRLX-ray2.40A/C1-355[»]
1LUCX-ray1.50A1-355[»]
3FGCX-ray2.30A/C1-355[»]
ProteinModelPortaliP07740.
SMRiP07740.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07740.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108M8W. Bacteria.
COG2141. LUCA.

Family and domain databases

CDDicd01096. Alkanal_monooxygenase. 1 hit.
Gene3Di3.20.20.30. 1 hit.
InterProiIPR033924. Alkanal_monooxygenase.
IPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like_dom.
IPR002103. Luciferase_bac/NFP.
[Graphical view]
PfamiPF00296. Bac_luciferase. 1 hit.
[Graphical view]
PRINTSiPR00089. LUCIFERASE.
SUPFAMiSSF51679. SSF51679. 1 hit.
PROSITEiPS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07740-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFGNFLLTY QPPELSQTEV MKRLVNLGKA SEGCGFDTVW LLEHHFTEFG
60 70 80 90 100
LLGNPYVAAA HLLGATETLN VGTAAIVLPT AHPVRQAEDV NLLDQMSKGR
110 120 130 140 150
FRFGICRGLY DKDFRVFGTD MDNSRALMDC WYDLMKEGFN EGYIAADNEH
160 170 180 190 200
IKFPKIQLNP SAYTQGGAPV YVVAESASTT EWAAERGLPM ILSWIINTHE
210 220 230 240 250
KKAQLDLYNE VATEHGYDVT KIDHCLSYIT SVDHDSNRAK DICRNFLGHW
260 270 280 290 300
YDSYVNATKI FDDSDQTKGY DFNKGQWRDF VLKGHKDTNR RIDYSYEINP
310 320 330 340 350
VGTPEECIAI IQQDIDATGI DNICCGFEAN GSEEEIIASM KLFQSDVMPY

LKEKQ
Length:355
Mass (Da):40,153
Last modified:August 1, 1988 - v1
Checksum:iF915E6BBE41ACEEE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17Q → E AA sequence (PubMed:3997817).Curated1
Sequence conflicti68T → K in CAA41597 (PubMed:1685011).Curated1
Sequence conflicti169P → A AA sequence (PubMed:3997817).Curated1
Sequence conflicti174A → P AA sequence (PubMed:3997817).Curated1
Sequence conflicti204 – 205QL → HV in CAA41597 (PubMed:1685011).Curated2
Sequence conflicti238R → K in CAA41597 (PubMed:1685011).Curated1
Sequence conflicti321D → N in CAA41597 (PubMed:1685011).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10961 Genomic DNA. Translation: AAA88685.1.
X58791 Genomic DNA. Translation: CAA41597.1.
V01422 Genomic DNA. Translation: CAA24692.1.
V01423 Genomic DNA. Translation: CAA24693.1.
PIRiA22613.
RefSeqiWP_010991819.1. NZ_LOSK01000002.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10961 Genomic DNA. Translation: AAA88685.1.
X58791 Genomic DNA. Translation: CAA41597.1.
V01422 Genomic DNA. Translation: CAA24692.1.
V01423 Genomic DNA. Translation: CAA24693.1.
PIRiA22613.
RefSeqiWP_010991819.1. NZ_LOSK01000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BRLX-ray2.40A/C1-355[»]
1LUCX-ray1.50A1-355[»]
3FGCX-ray2.30A/C1-355[»]
ProteinModelPortaliP07740.
SMRiP07740.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1518824.
STRINGi338187.VIBHAR_06242.

Chemistry databases

BindingDBiP07740.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108M8W. Bacteria.
COG2141. LUCA.

Enzyme and pathway databases

BRENDAi1.14.14.3. 6632.

Miscellaneous databases

EvolutionaryTraceiP07740.

Family and domain databases

CDDicd01096. Alkanal_monooxygenase. 1 hit.
Gene3Di3.20.20.30. 1 hit.
InterProiIPR033924. Alkanal_monooxygenase.
IPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like_dom.
IPR002103. Luciferase_bac/NFP.
[Graphical view]
PfamiPF00296. Bac_luciferase. 1 hit.
[Graphical view]
PRINTSiPR00089. LUCIFERASE.
SUPFAMiSSF51679. SSF51679. 1 hit.
PROSITEiPS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLUXA_VIBHA
AccessioniPrimary (citable) accession number: P07740
Secondary accession number(s): Q56698, Q6LBZ4, Q6LBZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The synthesis of this protein is regulated by a complex control mechanism that has been termed autoinduction. In fully induced cells luciferase comprises up to 5% of the soluble protein.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.