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Protein

Alkanal monooxygenase alpha chain

Gene

luxA

Organism
Vibrio harveyi (Beneckea harveyi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Light-emitting reaction in luminous bacteria.

Catalytic activityi

RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase, Photoprotein

Keywords - Biological processi

Luminescence

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BRENDAi1.14.14.3. 6632.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkanal monooxygenase alpha chain (EC:1.14.14.3)
Alternative name(s):
Bacterial luciferase alpha chain
Gene namesi
Name:luxA
OrganismiVibrio harveyi (Beneckea harveyi)
Taxonomic identifieri669 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Alkanal monooxygenase alpha chainPRO_0000220172Add
BLAST

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Protein-protein interaction databases

MINTiMINT-1518824.
STRINGi338187.VIBHAR_06242.

Chemistry

BindingDBiP07740.

Structurei

Secondary structure

1
355
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi17 – 3014Combined sources
Helixi32 – 343Combined sources
Beta strandi37 – 415Combined sources
Helixi55 – 6511Combined sources
Beta strandi70 – 778Combined sources
Helixi78 – 803Combined sources
Helixi83 – 9715Combined sources
Beta strandi101 – 1066Combined sources
Helixi111 – 1177Combined sources
Helixi121 – 1233Combined sources
Helixi124 – 14118Combined sources
Beta strandi142 – 1509Combined sources
Beta strandi152 – 1576Combined sources
Beta strandi170 – 1723Combined sources
Helixi177 – 1859Combined sources
Beta strandi190 – 1923Combined sources
Beta strandi194 – 1963Combined sources
Helixi198 – 21417Combined sources
Helixi219 – 2213Combined sources
Beta strandi225 – 2295Combined sources
Helixi236 – 25924Combined sources
Turni260 – 2634Combined sources
Helixi274 – 2763Combined sources
Helixi294 – 2985Combined sources
Beta strandi299 – 3035Combined sources
Helixi304 – 31815Combined sources
Beta strandi322 – 3265Combined sources
Helixi328 – 3303Combined sources
Helixi333 – 34614Combined sources
Helixi348 – 3503Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BRLX-ray2.40A/C1-355[»]
1LUCX-ray1.50A1-355[»]
3FGCX-ray2.30A/C1-355[»]
ProteinModelPortaliP07740.
SMRiP07740. Positions 1-355.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07740.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108M8W. Bacteria.
COG2141. LUCA.

Family and domain databases

Gene3Di3.20.20.30. 1 hit.
InterProiIPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like_dom.
IPR002103. Luciferase_bac.
[Graphical view]
PfamiPF00296. Bac_luciferase. 1 hit.
[Graphical view]
PRINTSiPR00089. LUCIFERASE.
SUPFAMiSSF51679. SSF51679. 1 hit.
PROSITEiPS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07740-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFGNFLLTY QPPELSQTEV MKRLVNLGKA SEGCGFDTVW LLEHHFTEFG
60 70 80 90 100
LLGNPYVAAA HLLGATETLN VGTAAIVLPT AHPVRQAEDV NLLDQMSKGR
110 120 130 140 150
FRFGICRGLY DKDFRVFGTD MDNSRALMDC WYDLMKEGFN EGYIAADNEH
160 170 180 190 200
IKFPKIQLNP SAYTQGGAPV YVVAESASTT EWAAERGLPM ILSWIINTHE
210 220 230 240 250
KKAQLDLYNE VATEHGYDVT KIDHCLSYIT SVDHDSNRAK DICRNFLGHW
260 270 280 290 300
YDSYVNATKI FDDSDQTKGY DFNKGQWRDF VLKGHKDTNR RIDYSYEINP
310 320 330 340 350
VGTPEECIAI IQQDIDATGI DNICCGFEAN GSEEEIIASM KLFQSDVMPY

LKEKQ
Length:355
Mass (Da):40,153
Last modified:August 1, 1988 - v1
Checksum:iF915E6BBE41ACEEE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171Q → E AA sequence (PubMed:3997817).Curated
Sequence conflicti68 – 681T → K in CAA41597 (PubMed:1685011).Curated
Sequence conflicti169 – 1691P → A AA sequence (PubMed:3997817).Curated
Sequence conflicti174 – 1741A → P AA sequence (PubMed:3997817).Curated
Sequence conflicti204 – 2052QL → HV in CAA41597 (PubMed:1685011).Curated
Sequence conflicti238 – 2381R → K in CAA41597 (PubMed:1685011).Curated
Sequence conflicti321 – 3211D → N in CAA41597 (PubMed:1685011).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10961 Genomic DNA. Translation: AAA88685.1.
X58791 Genomic DNA. Translation: CAA41597.1.
V01422 Genomic DNA. Translation: CAA24692.1.
V01423 Genomic DNA. Translation: CAA24693.1.
PIRiA22613.
RefSeqiWP_010991819.1. NZ_LOSK01000002.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10961 Genomic DNA. Translation: AAA88685.1.
X58791 Genomic DNA. Translation: CAA41597.1.
V01422 Genomic DNA. Translation: CAA24692.1.
V01423 Genomic DNA. Translation: CAA24693.1.
PIRiA22613.
RefSeqiWP_010991819.1. NZ_LOSK01000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BRLX-ray2.40A/C1-355[»]
1LUCX-ray1.50A1-355[»]
3FGCX-ray2.30A/C1-355[»]
ProteinModelPortaliP07740.
SMRiP07740. Positions 1-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1518824.
STRINGi338187.VIBHAR_06242.

Chemistry

BindingDBiP07740.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108M8W. Bacteria.
COG2141. LUCA.

Enzyme and pathway databases

BRENDAi1.14.14.3. 6632.

Miscellaneous databases

EvolutionaryTraceiP07740.

Family and domain databases

Gene3Di3.20.20.30. 1 hit.
InterProiIPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like_dom.
IPR002103. Luciferase_bac.
[Graphical view]
PfamiPF00296. Bac_luciferase. 1 hit.
[Graphical view]
PRINTSiPR00089. LUCIFERASE.
SUPFAMiSSF51679. SSF51679. 1 hit.
PROSITEiPS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLUXA_VIBHA
AccessioniPrimary (citable) accession number: P07740
Secondary accession number(s): Q56698, Q6LBZ4, Q6LBZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: May 11, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The synthesis of this protein is regulated by a complex control mechanism that has been termed autoinduction. In fully induced cells luciferase comprises up to 5% of the soluble protein.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.